中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (5): 863-866.doi: 10.3969/j.issn.1673-8225.2012.05.025

• 器官移植基础实验 basic experiments of organ transplantation • 上一篇    下一篇

甘油常温保存肌腱的两种方法比较*★

韩  冰，宋一平，王和洪，陈  烁，童  讯，赵日光，孙燚炎，冯  晖   

  1. 解放军第97医院南京军区创伤修复中心，江苏省徐州市  221004
  • 收稿日期:2011-07-30 修回日期:2011-10-20 出版日期:2012-01-29 发布日期:2012-01-29
  • 通讯作者: 宋一平，主任医师，解放军第97医院南京军区创伤修复中心，江苏省徐州市 221004 songyipingjsxz@163.com
  • 作者简介:韩冰★，男，1973年生，江苏省徐州市人，汉族，2006年徐州医学院毕业，硕士，医师，主要从事干细胞培养与移植、组织工程方面的研究。hanbingjsxz@163.com
  • 基金资助:

    南京军区医学科学技术研究“十一五”计划课题(06z17)，课题名称：同种异体肌腱、血管、神经的保存及保存液的研究及临床应用

Comparison of two methods of tendon preservation at normal temperature in glycerine

Han Bing, Song Yi-ping, Wang He-hong, Chen Shuo, Tong Xun, Zhao Ri-guang, Sun Yi-yan, Feng Hui   

  1. Wound Healing Centers of Nanjing Military Region, the 97th Hospital of Chinese PLA, Xuzhou  221004, Jiangsu Province, China
  • Received:2011-07-30 Revised:2011-10-20 Online:2012-01-29 Published:2012-01-29
  • Contact: Song Yi-ping, Chief physician, Wound Healing Centers of Nanjing Military Region, the 97th Hospital of Chinese PLA, Xuzhou 221004, Jiangsu Province, China songyipingjsxz@163.com
  • About author:Han Bing★, Master, Physician, Wound Healing Centers of Nanjing Military Region, the 97th Hospital of Chinese PLA, Xuzhou 221004, Jiangsu Province, China hanbingjsxz@163.com
  • Supported by:

    “Eleventh Five-Year” Plan Project of Medical Science and Technology Research of Nanjing Military Region, No.06z17*

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摘要：

背景：最大程度保留肌腱的生物活性是进行同种异体肌腱移植的条件。
目的：通过比较选择出最佳的肌腱常温保存方法。
方法：使用无菌操作的方法将兔肌腱随机分为4组：新鲜肌腱对照组、生理盐水组、无水甘油Ⅰ组(肌腱梯度脱水后在无水甘油中保存)、无水甘油Ⅱ组(肌腱直接在无水甘油中保存)，分别于保存的第2，4，7个月进行检测。
结果与结论：苏木精-伊红染色显示：无水甘油Ⅰ组肌腱细胞完整率明显高于无水甘油Ⅱ组。电镜观察发现，无水甘油Ⅰ组大部分肌腱细胞形态正常，肌腱组织结构完整；无水甘油Ⅱ组保存4，7个月后细胞核凝固、固缩、崩解状。无水甘油Ⅰ组的超氧化物歧化酶活性也明显高于无水甘油Ⅱ组。说明肌腱经梯度脱水后在无水甘油中常温保存效果更佳。

关键词: 肌腱, 活性, 甘油, 常温保存, 脱水, 比较

Abstract:

BACKGROUND: The greatest degree to obtain the biological activity of tendon is the conditions for allograft tendon transplantation.
OBJECTIVE: To select the best tendon preservation method at room temperature.
METHODS: The rabbit tendons were randomly divided into four groups using the aseptic technique: Control group of fresh tendon, normal saline group, anhydrous glycerol group Ⅰ(tendons were preserved in anhydrous glycerol after gradient dehydration), anhydrous glycerol group Ⅱ (tendons were preserved in anhydrous glycerol directly). Tendons in each group were preformed with examination at 2, 4 and 7 months.
RESULTS AND CONCLUSION: Hematoxylin-eosin staining showed that the cell integrity of anhydrous glycerol group Ⅰ was higher than that of anhydrous glycerol group Ⅱ. Electron microscope observation showed that the morphology of most tendon cells in anhydrous glycerol group Ⅰ was normal, the structure of tendon tissue was integrated; After the tendons were preserved in anhydrous glycerol group Ⅱ for 4 and 7 months, the nucleus were coagulation, condensation and collapse-like. The activity of superoxide dismutase in anhydrous glycerol group Ⅰ was obviously higher than that in the anhydrous glycerol group Ⅱ. It indicates that the tendon preformed with gradient dehydration can be preserved in anhydrous glycerol effectively.  

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