中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (5): 652-654.doi: 10.3969/j.issn.1673-8225.2012.05.022

• 器官移植基础实验 basic experiments of organ transplantation •    下一篇

鱼藤素对斑马鱼胚胎毒性的作用*★

李文浩1，吴新荣1，2   

  1. 1华南理工大学轻工与食品学院，广东省广州市  510641；2解放军广州军区广州总医院药剂科，广东省广州市 510010
  • 收稿日期:2011-09-30 修回日期:2011-12-16 出版日期:2012-01-29 发布日期:2012-01-29
  • 通讯作者: 吴新荣，博士，主任药师，博士生导师，华南理工大学轻工与食品学院，广东省广州市 510641 gzwxrong@yahoo.com.cn
  • 作者简介:2011-09-30李文浩★，男，1986年生，广东省五华市人，华南理工大学在读硕士，主要从事抗肿瘤药物方面的研究。 lwh288@qq.com
  • 基金资助:

    广东省自然科学基金(10251001002000000)，课题名称：基于flk-GFP斑马鱼模型的鱼藤素血管生成抑制作用机制研究。

Embryotoxic effect of deguelin in zebrafish

Li Wen-hao1, Wu Xin-rong1, 2   

  1. 1College of Light Industry and Food Sciences, South China University of Technology, Guangzhou  510641, Guangdong Province, China; 2Department of Pharmacy, Guangzhou General Hospital of Guangzhou Military Area Command of Chinese PLA, Guangzhou  510010, Guangdong Province, China
  • Received:2011-09-30 Revised:2011-12-16 Online:2012-01-29 Published:2012-01-29
  • Contact: Wu Xin-rong, Doctor, Chief pharmacist, Master’s supervisor, College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510641, Guangdong Province, China; Department of Pharmacy, Guangzhou General Hospital of Guangzhou Military Area Command of Chinese PLA, Guangzhou 510010, Guangdong Province, China gzwxrong@yahoo.com.cn
  • About author:Li Wen-hao★, Studying for master’s degree, College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510641, Guangdong Province, China lwh288@qq.com
  • Supported by:
    Natural Science Foundation of Guangdong, No.10251001002000000*
     

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摘要：

背景：以模式生物斑马鱼构建生物模型，易于开展活体实验。
目的：探讨了不同鱼藤素浓度对斑马鱼胚胎发育的影响。
方法：不同鱼藤素浓度作用于斑马鱼胚胎，分别在受精后48，72 h观测其表型变化，受精后72 h统计其胚胎孵化率。
结果与结论：鱼藤素在0.1 μmol/L浓度以下对斑马鱼胚胎发育无影响，0.2 μmol/L浓度延缓斑马鱼胚胎发育，0.3 μmol/L浓度以上抑制了斑马鱼胚胎发育，使胚胎致死。结果表明，鱼藤素高浓度使胚胎致死，低浓度延缓胚胎发育。
 

关键词: 鱼藤素, 斑马鱼, 胚胎发育, 帕金森病, 肿瘤细胞

Abstract:

Embryotoxic effect of deguelin in zebrafish BACKGROUND: The establishment of antitumor drug deguelin model provides basis for the in vivo experiment.
OBJECTIVE: To investigate the influence of different concentrations of deguelin on zebrafish embryonic development.
METHODS: The zebrafish embryos were treated with different concentrations of deguelin. The changes of the phenotype were observed at 48 and 72 hours after postfertilization, and the hatching rate was counted at 72 hours after postfertilization.
RESULTS AND CONCLUSION: The development of embryo was remained under 0.1 μmol/L concentration of deguelin. The development of embryo was delayed at 0.2 μmol/L concentration of deguelin. 0.3 μmol/L concentration of deguelin could inhibit the development of embryo and cause death of the embryo. These results show that high concentration of deguelin leads to embryonic lethality, while low concentration of deguelin delays the embryonic development.

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