中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (3): 467-470.doi: 10.3969/j.issn.1673-8225.2012.03.019

• 材料生物相容性 material biocompatibility • 上一篇    下一篇

新型纳米拓扑结构的生物相容性**★◆

刘继春1，许  鹏1，余将明1，浦东林2，叶晓健1   

  1. 1解放军第二军医大学长征医院，上海市  200043；  2苏州大学先进光学制造技术重点实验室，江苏省苏州市  215000
  • 收稿日期:2011-07-01 修回日期:2011-09-19 出版日期:2012-01-15 发布日期:2012-01-15
  • 通讯作者: 叶晓健，教授，博士生导师，副院长，解放军第二军医大学长征医院，上海市 200043 yexj2002@ 163.com
  • 作者简介:刘继春★，男，1983年生，安徽省淮北市人，汉族，硕士，现在解放军第184医院骨科工作，主要从事脊柱外科方面研究。 3-9-8-1@ 163.com
  • 基金资助:

    国家自然科学基金(81071477)，名称：wnt/β-catenin信号通路在纳米拓扑结构诱导骨髓间充质干细胞向成骨细胞分化中的作用和机理研究；973计划课题(2009CB930000)，名称：面向组织修复与替代的纳米生物材料的研究。

Biocompatibility of new nanotopograpgy 

Liu Ji-chun1, Xu Peng1, Yu Jiang-ming1, Pu Dong-lin2, Ye Xiao-jian1   

  1. 1Shanghai Changzheng Hospital, the Second Military Medical University, Shanghai  200043, China; 2Key Laboratory of Advanced Optical Manufacturing Technology, Soochow University, Suzhou  215000, Jiangsu Province, China
  • Received:2011-07-01 Revised:2011-09-19 Online:2012-01-15 Published:2012-01-15
  • Contact: Ye Xiao-jian, Professor, Doctoral supervisor, Shanghai Changzheng Hospital, the Second Military Medical University, Shanghai 200043, China yexj2002@163.com yexj2002@163.com
  • About author:Ye Xiao-jian, Professor, Doctoral supervisor, Shanghai Changzheng Hospital, the Second Military Medical University, Shanghai 200043, China yexj2002@163.com
  • Supported by:

    National Natural Science Foundation of China, No. 81071477*; Major State Basic Research Development Program, No. 2009CB930000*

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摘要：

背景：国内外对纳米拓扑结构生物效应的探讨比较多，而缺乏对纳米拓扑结构的安全性评估。
目的：评价新型纳米拓扑结构的生物相容性。
方法：依据GB/T_16886中相关规定，采用纳米拓扑结构材料对昆明小鼠、新西兰大白兔、人行全身毒性试验、刺激与致敏试验及急性溶血试验。分离培养兔骨髓间充质干细胞复合培养于纳米拓扑结构，观察细胞的形态及增殖情况。
结果与结论：全身毒性试验结果显示，所有试验动物均无死亡，试验前后体质量无明显差异；刺激与致敏试验中，所有试验动物局部皮肤均无红斑、溃烂等反应，阳性对照组与试验组之间差异有显著性意义；急性溶血试验结果显示，试验组无明显溶血反应，吸光光度计检测吸光度，溶血率符合小于5%的标准。体外细胞试验结果显示，骨髓间充质干细胞在此纳米坑上表现出良好的黏附、细胞形态，并且复合培养3 d较1 d细胞增殖明显。提示此纳米坑结构无生物学毒性，具有良好的生物相容性，可以作为骨科替代植入物试用于临床，但长期生物相容性仍需要全面的评测。
关键词：纳米拓扑结构；纳米生物材料；生物相容性；急性毒性试验；刺激与致敏试验；急性溶血试验；体外细胞试验
doi:10.3969/j.issn.1673-8225.2012.03.019

关键词: 纳米拓扑结构, 纳米生物材料, 生物相容性, 急性毒性试验, 刺激与致敏试验, 急性溶血试验, 体外细胞试验

Abstract:

BACKGROUND: There are many studies about biological effect of nanotopography, but the research of safety evaluation of nanotopography has not been reported.
OBJECTIVE: To evaluate the biocompatibility of new nanotopography.
METHODS: The experiments of systemic toxicity, irritation and sensitization, and acute hemolysis of nanotopography were performed in Kunming mice, New Zealand rabbits and human according to “GB/T_16886”. The morphology and proliferation of rabbit bone marrow mesenchymal stem cells cultured on nanotopography were observed.
RESULTS AND CONCLUSION: All test animals were alive. Acute toxicity: there was no significant difference in weight before and after experiment. Tests for irritation and sensitization: there were no erythema or fester on surfaces of test animals, and the difference between the experimental group and control group was significant. Acute hemolysis: hemolytic reaction could not be observed in the experimental group, and hemolytic rate was less than 5% according to absorbance. Well cell morphology and adhesion of bone marrow mesenchymal stem cells cultured on nanotopography were observed by experiments in vitro, and cell proliferation at 1 day was increased compared with that at 3 days. All the results suggested that nanotopography is no-biotoxicity and has a good biocompatibility. Therefore, it can be used as orthopedic substitute implant in clinic. However, its long-term effect of biotoxicity needs to be evaluated.

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