中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (3): 463-499.doi: 10.3969/j.issn.1673-8225.2012.03.018

• 药物控释材料 drug delivery materials • 上一篇    下一篇

胶原润眼液能够加快眼角膜上皮损伤的修复*★

雷  静1，陈淡嫦1，黄冬晶1，宋  鄂2，杨  威2，孙雅彬2   

  1. 1广州创尔生物技术有限公司，广东省广州市 510663；2吉林大学，吉林省长春市 130012
  • 收稿日期:2011-05-13 修回日期:2011-06-16 出版日期:2012-01-15 发布日期:2012-01-15
  • 作者简介:雷静★，女，1980年生，湖北省襄樊市人，汉族，2005年华东师范大学毕业，硕士，助理研究员，主要从事生物医用材料、医疗器械及分子免疫学研究。jairsa@126.com jairsa@126.com
  • 基金资助:

    广东省省部产学研结合项目基金资助项目(2009B090300461)。

Collagen eye drop improves corneal wound healing of rabbits 

Lei Jing1, Chen Dan-chang1, Huang Dong-jing1, Song E2, Yang Wei2, Sun Ya-bin2     

  1. 1Guangzhou Trauer Biotechnology Co., Ltd., Guangzhou  510663, Guangdong Province, China; 2Jilin University, Changchun  130012, Jilin Province, China
  • Received:2011-05-13 Revised:2011-06-16 Online:2012-01-15 Published:2012-01-15
  • About author:Lei Jing★, Master, Assistant researcher, Guangzhou Trauer Biotechnology Co., Ltd., Guangzhou 510663, Guangdong Province, China jairsa@126.com
  • Supported by:

    the Combination Project Fund of Production, Learning and Research of Guangdong Province, No. 2009B090300461* 
       

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摘要：

背景：Ⅰ型胶原不仅是角膜的主要组成成分，还能对受伤角膜起到营养修复作用，具有很好的药物缓释功能。
目的：了解胶原润眼液对兔损伤角膜的修复作用，并评价其生物安全性。
方法：采用角膜环钻制备兔双眼角膜损伤模型，抗感染处理后，右眼滴胶原润眼液作为实验组，左眼滴生理盐水作为对照组，以结膜分泌物及水肿度、2%荧光素角膜染色评分、损伤愈合率等指标评价胶原润眼液对损伤角膜的修复作用。
结果与结论：对照组兔术后结膜分泌物增多，水肿严重；实验组动物术后结膜分泌物较少，无严重水肿和充血。实验组术后第11天，13天角膜损伤愈合率明显高于对照组(P < 0.05)。体外细胞毒性试验不大于2级反应，且无致敏和刺激反应，结果显示胶原润眼液无生物学危害，能够加快兔损伤角膜的愈合。
关键词：胶原润眼液；角膜；修复；安全性；组织工程
doi:10.3969/j.issn.1673-8225.2012.03.018
 

关键词: 胶原润眼液, 角膜, 修复, 安全性, 组织工程

Abstract:

BACKGROUND: Type I collagen is not only a major component of the cornea, but also can repair the injured cornea and play the role of nutrition, with a good drug delivery capability.
OBJECTIVE: To understand the effect of collagen eye drop on the repairing of injured rabbit’s corneal and to evaluate the biological safety.   
METHODS: Corneal Ring Drill was used for rabbit cornea injury model preparation. Anti-infection treatment was performed after surgery. Right eyes treated with collagen eye drop were used as experimental group, and left eyes treated with normal saline served as controls. Secretion and edema degrees, 2% fluorescent element corneal dyeing ratings, damage healing rates were used for evaluation of the effect of collagen eye drop on cornea injury.
RESULTS AND CONCLUSION: The conjunctival secretions in the experimental group were greatly lower than that in the control group. Compared with the control group, the edema and congestions of rabbits after surgery were lighter in the experimental group. The healing rates of the cornea lesion at 11 and 13 days after surgery in the experimental group were significantly higher than those in the control group (P < 0.05). In vitro cytotoxicity test showed not more than 2 levels of reaction. And there was no allergic reaction, and the results showed that the collagen eye drops had no biological hazards and could accelerate the wound healing of the cornea.

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