中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (3): 434-437.doi: 10.3969/j.issn.1673-8225.2012.03.012

• 组织工程骨及软骨材料 tissue-engineered bone and cartilage materials • 上一篇    下一篇

异种脱钙骨基质联合骨髓间充质干细胞植入豚鼠鼓泡腔增强成骨作用*★

段礼府1，王海涛2，李化静1，马  艳3，刘立中1   

  1. 新疆医科大学第一附属医院，1耳鼻咽喉科，2影像中心，3医学研究中心，新疆维吾尔自治区乌鲁木齐市   830054
  • 收稿日期:2011-07-29 修回日期:2011-10-20 出版日期:2012-01-15 发布日期:2012-01-15
  • 通讯作者: 刘立中，博士，教授，新疆医科大学第一附属医院耳鼻咽喉科，新疆维吾尔自治区乌鲁木齐市 830054 lulizhongno. 1@163.com
  • 作者简介:段礼府★，男，1982年生，四川省南充市人，汉族，新疆医科大学在读硕士，主要从事耳科组织工程与干细胞治疗研究。 1458668171@ qq.com
  • 基金资助:

    新疆医科大学研究生创新基金(MCR010-7)。

Enhanced osteogenesis of implantation of heterogeneous demineralized bone matrix combined with bone marrow mesenchymal stem cells into the tympanic bullas of guinea pigs  

Duan Li-fu1, Wan Hai-tao2, Li Hua-jing1, Ma Yan3, Liu Li-zhong1   

  1. 1Department of Otorhinolaryngology, First Affiliated Hospital of Xinjiang Medical University, Urumqi  830054, Xinjiang Uygur Autonomous Region, China; 2Imaging Center, First Affiliated Hospital of Xinjiang Medical University, Urumqi  830054, Xinjiang Uygur Autonomous Region, China; 3Medical Research Center, First Affiliated Hospital of Xinjiang Medical University, Urumqi  830054, Xinjiang Uygur Autonomous Region, China
  • Received:2011-07-29 Revised:2011-10-20 Online:2012-01-15 Published:2012-01-15
  • Contact: Liu Li-zhong, Doctor, Professor, Department of Otorhinolaryngology, First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China lulizhongno.1@ 163.com
  • About author:Duan Li-fu★, Studying for master’s degree, Department of Otorhinolaryngology, First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China 1458668171@ qq.com
  • Supported by:

    Graduate Innovation Foundation of Xinjiang Medical University, No. MCR010-7*

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摘要：

背景：目前在矫形外科和颅面重建中已有脱钙骨基质和骨髓间充质干细胞的相关应用，但用于乳突腔填塞尚缺乏研究。
目的：探寻异种脱钙骨基质和骨髓间充质干细胞联合植入乳突腔的成骨效能。
方法：全骨髓贴壁培养法分离近交系雌性豚鼠骨髓间充质干细胞，牛股骨头松质骨制备牛髂骨脱钙骨基质。20只豚鼠随机数字表法分为脱钙骨基质与骨髓间充质干细胞联合植入鼓泡腔的实验组和取自体髂骨植入鼓泡腔的对照组。
结果与结论：植入1个月后两组的成骨量差异无显著性意义；植入后2个月实验组成骨量多于对照组，差异有显著性意义    (P < 0.05)。说明异种脱钙骨基质与骨髓间充质干细胞联合植入乳突腔能有效促进乳突填塞后骨的形成。

关键词: 异种脱钙骨基质, 骨髓间充质干细胞, 颅面重建, 乳突填塞, 自体骨移植

Abstract:

BACKGROUND: Currently, there are studies on the related applications of demineralized bone matrix and bone marrow mesenchymal stem cells (BMSCs) in orthopedic surgery and craniofacial reconstruction. However, the application in mastoid obliteration is still unclear.
OBJECTIVE: To explore the osteogenic effectiveness of heterogeneous demineralized bone matrix combined with BMSCs implanted into the mastoid cavity.
METHODS: Inbred female guinea pig BMSCs were isolated with the whole bone marrow adherent culture method, and the demineralized bone matrix was prepared with cancellous bone of bovine femoral head. Twenty guinea pigs were equally divided into two groups: the experimental group was implanted by demineralized bone matrix combined with BMSCs into the tympanic bulla, and the control group was treated with implantation of autologous lliac into the tympanic bulla.
RESULTS AND CONCLUSION: The new bone quantity in the two groups had no significant difference at 1 month after implantation; 2 months later, the new bone quantity in the experimental group was significantly higher than that of the control group (P < 0.05). It is indicated that the implantation of heterogeneous demineralized bone matrix combined with BMSCs into the mastoid cavity can effectively promote the osteogenesis after mastoid obliteration.

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