中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (1): 61-64.doi: 10.3969/j.issn.1673-8225.2012.01.013

• 干细胞因子及调控因子 stem cell factors and regulatory factors • 上一篇    下一篇

长期培养对基质细胞衍生因子1/CXCR4介导间充质干细胞迁移的影响**★◆

王  瑾，黄梁浒，王庆华，施小华，董会月，谭建明   

  1. 解放军南京军区福州总医院福建省移植生物学重点实验室，福建省福州市  350025
  • 收稿日期:2011-05-01 修回日期:2011-07-27 出版日期:2012-01-01 发布日期:2012-01-01
  • 通讯作者: 谭建明，博士生导师，解放军南京军区福州总医院福建省移植生物学重点实验室，福建省福州市350025 tanjianming59@yahoo.com.cn
  • 作者简介:王瑾★，女，1982年生，福建省福州市人，汉族，2008年中山大学毕业，硕士，主要从事干细胞生物学研究。jeanwang0626@yahoo.com.cn
  • 基金资助:

    福建省科技计划重大项目(2009Y4001)和福建省科技创新平台建设计划(2010Y2006)。

Stromal cell-derived factor-1/CXCR4 effects on ex vivo expanded bone marrow mesenchymal stem cells migration

Wang Jin, Huang Liang-hu, Wang Qing-hua, Shi Xiao-hua, Dong Hui-yue, Tan Jian-ming   

  1. Fujian Provincial Key Laboratory of Transplant Biology, Fuzhou General Hospital of Nanjing Military Command, Fuzhou  350025, Fujian Province, China
  • Received:2011-05-01 Revised:2011-07-27 Online:2012-01-01 Published:2012-01-01
  • Contact: Tan Jian-ming, Doctoral supervisor, Professor, Fujian Provincial Key Laboratory of Transplant Biology, Fuzhou General Hospital of Nanjing Military Command, Fuzhou 350025, Fujian Province, China tanjianming59@yahoo.com.cn
  • About author:Wang Jin★, Master, Fujian Provincial Key Laboratory of Transplant Biology, Fuzhou General Hospital of Nanjing Military Command, Fuzhou 350025, Fujian Province, China jeanwang0626@yahoo.com.cn
  • Supported by:

    the Major Science and Technology Program of Fujian Province, No. 2009Y4001*; the Technological Innovation Platform Plan of Fujian Province, No. 2010Y2006*
       

PDF

285

被引次数

6

摘要：

背景：人外源性的间充质干细胞能特异性地向损伤部位迁移，参与多种组织的损伤修复，然而其定向迁移的机制尚不清楚。
目的：观察基质细胞衍生因子1对骨髓间充质干细胞定向迁移的影响。
方法：体外培养不同个体的骨髓间充质干细胞，培养至第10代，检测细胞衰老状态。RT-PCR和细胞免疫荧光检测骨髓间充质干细胞CXCR4受体表达情况；体外迁移体系(Transwell)检测基质细胞衍生因子1对骨髓间充质干细胞迁移的影响。
结果与结论：骨髓间充质干细胞在体外长期增殖后生长逐渐缓慢，在第6，7代衰老细胞增多，其CXCR4受体表达减少。基质细胞衍生因子1对骨髓间充质干细胞的趋化作用呈剂量依赖性。
关键词：趋化作用；骨髓间充质干细胞；细胞衰老；基质细胞衍生因子1；CXCR4受体
doi:10.3969/j.issn.1673-8225.2012.01.013
 

关键词: 趋化作用, 骨髓间充质干细胞, 细胞衰老, 基质细胞衍生因子1, CXCR4受体

Abstract:

BACKGROUND: Exogenous human mesenchymal stem cells (MSCs) possess an ability to selectively migrate to the site of injury, and may further repair various damaged tissue. The mechanisms of directional migration remain unclear.
OBJECTIVE: To examine the expression of the CXCR4 receptor in MSCs after long-term culture ex vivo, and to investigate the effects of stromal cell derived factor-1 (SDF-1) at various concentrations on MSCs migration.
METHODS: MSCs were cultured ex vivo to passage 10, and then cell senescence was determined by β-galactosidase staining. The expression of CXCR4 receptor in MSCs was examined by RT-PCR and immunofluorescence staining. Transwell system was used to detect in vitro migration capacity of MSCs towards SDF-1.
RESULTS AND CONCLUSION: Proliferation gradually decreased in all samples in the course of long-term culture ex vivo, and became restricted to senescent stages at passage 6 or 7. The expression of CXCR4 receptor was gradually lost with culture expansion. SDF-1 induced the migration of MSCs in a dose-dependent manner.
Wang J, Huang LH, Wang QH, Shi XH, Dong HY, Tan JM. Stromal cell-derived factor-1/CXCR4 effects on ex vivo expanded bone marrow mesenchymal stem cells migration.Zhongguo Zuzhi Gongcheng Yanjiu. 2012;16(1): 61-64.     [http://www.crter.cn  http://en.zglckf.com]

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