中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (49): 9223-9226.doi: 10.3969/j.issn.1673-8225.2011.49.024

• 干细胞基础实验 basic experiments of stem cells • 上一篇    下一篇

体外诱导胎盘间充质干细胞向表皮细胞的分化

万振洲1，冯亚松1，彭海林2，张  玮1，苗宗宁3，陈亚宝2   

  1. 1泰州市第四人民医院检验科，江苏省泰州市225300
    2泰州市人民医院检验科，江苏省泰州市  225300
    3无锡市第三人民医院干细胞实验室，江苏省无锡市 214000
  • 收稿日期:2011-06-26 修回日期:2011-11-10 出版日期:2011-12-03 发布日期:2011-12-03
  • 作者简介:万振洲，男，1975年生，江苏省泰州市人，汉族，1998年镇江医学院毕业，主管检验师，主要从事分子生物学的研究。 13901433616@139.com

Differentiation of placenta mesenchymal stem cells to epidermal cells in vitro

Wan Zhen-zhou1, Feng Ya-song1, Peng Hai-lin2, Zhang Wei1, Miao Zong-ning3, Chen Ya-bao2   

  1. 1Medical Laboratory of Taizhou Fourth People's Hospital, Taizhou  225300, Jiangsu Province, China
    2Medical Laboratory of Taizhou People's Hospital, Taizhou  225300, Jiangsu Province, China
    3Stem Cell Laboratory of Wuxi Third People's Hospital, Wuxi  214000, Jiangsu Province, China
  • Received:2011-06-26 Revised:2011-11-10 Online:2011-12-03 Published:2011-12-03
  • About author:Wan Zhen-zhou, Docimaster in charge, Medical Laboratory of Taizhou Fourth People's Hospital, Taizhou 225300, Jiangsu Province, China 13901433616@139.com

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452

被引次数

11

摘要：

背景：以往研究证明胎盘间充质干细胞经诱导可以向骨、软骨、肌肉、脂肪等间充质细胞分化。
目的：探讨体外诱导胎盘间充质干细胞分化为表皮细胞的可行性。
方法：分离培养胎盘间充质干细胞，流式细胞仪鉴定其表面抗原，用含体积分数为2%胎牛血清以及20 μg/L表皮生长因子条件培养基诱导，诱导后细胞通过表皮细胞标志物CK19以及CK10染色鉴定。
结果与结论：胎盘间充质干细胞表面抗原CD29、CD44、CD105阳性，CD34、CD45、CD106以及HLA-DR阴性。诱导后细胞形态明显改变，表皮细胞标志物CK19以及CK10免疫荧光染色阳性，提示胎盘间充质干细胞在体外具有分化为表皮细胞的能力，可以作为皮肤组织工程和干细胞研究的一种有效来源。

关键词: 胎盘, 表皮细胞, 间充质干细胞, 诱导, 分化

Abstract:

BACKGROUND: The pervious study has showed that placenta mesenchymal stem cells (PMSCs) can differentiate into bone, cartilage, and muscle and adipose mesenchymal stem cells.
OBJECTIVE: To investigate the differentiation of PMSCs into epidermal cells in vitro.
METHODS: PMSCs were isolated and cultured and the surface antigen was detected by flow cytometry.
RESULTS AND CONCLUSION: Isolated PMSCs were positive for the markers CD105, CD29 and CD44 and negative for typical hematopoietic and endothelial markers CD34, CD45, CD106 and HLA-DR. The morphology of induced cells was obviously changed. Immunohistochemistry stain analysis showed positive for epidermal-specific markers like CK19 and CK10. The PMSCs have the capability to differentiate into epidermal cells in vitro. The placenta may prove to be a useful source of mesenchymal stem cells in skin tissue engineering and stem cells study.

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