中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (49): 9141-9145.doi: 10.3969/j.issn.1673-8225.2011.49.005

• 骨髓干细胞 bone marrow stem cells • 上一篇    下一篇

胶质源性神经营养因子基因修饰骨髓间充质干细胞与缺氧复氧神经细胞的共培养

熊怀林1，邓  莉2，高小青2，王特为2，郭  侃2，杨朝鲜2   

  1. 泸州医学院，1解剖教研室；2神经生物学研究室，四川省泸州市 646000
  • 收稿日期:2011-06-16 修回日期:2011-08-10 出版日期:2011-12-03 发布日期:2011-12-03
  • 通讯作者: 杨朝鲜，教授，硕士生导师，泸州医学院神经生物学研究室，四川省泸州市 646000 Yangchaoxian2006@tom.com
  • 作者简介:熊怀林☆，女，1975年生，四川省泸县人，汉族，四川大学在读博士，讲师，主要从事脑损伤及其修复的研究。 497101029@qq.com
  • 基金资助:

    四川省科技厅资助项目(2006J13-005-1)，四川省教育厅资助项目(2006A155)。

Bone marrow mesenchymal stem cells modified by glial cell line-derived neural factor gene co-cultrued with neurons subjected to anoxia-reoxygenation

Xiong Huai-lin1, Deng Li 2, Gao Xiao-qing2, Wang Te-wei2, Guo Kan2, Yang Chao-xian 2   

  1. 1Department of Anatomy, 2Department of Neurobiology, Luzhou Medical College, Luzhou 646000, Sichuan Province, China
  • Received:2011-06-16 Revised:2011-08-10 Online:2011-12-03 Published:2011-12-03
  • Contact: Yang Chao-xian, Professor, Master’s supervisor, Department of Neurobiology, Luzhou Medical College, Luzhou 646000, Sichuan Province, China yangchaoxian2006@tom.com
  • About author:Xiong Huai-lin☆, Studying for doctorate, Lecturer, Department of Anatomy, Luzhou Medical College, Luzhou 646000, Sichuan Province, China 497101029@qq.com
  • Supported by:

     the Foundation of Sichuan Science and Technology Department, No.2006J13-005-1*;  the Foundation of Sichuan Educational Department, No. 2006A155*

PDF

388

被引次数

5

摘要：

背景：课题组前期研究表明，胶质源性神经营养因子基因修饰的骨髓间充质干细胞能稳定地表达胶质源性神经营养因子 mRNA，但骨髓间充质干细胞/胶质源性神经营养因子分泌的胶质源性神经营养因子蛋白对缺氧复氧损伤的神经细胞是否有积极影响，尚不清楚。
目的：观察胶质源性神经营养因子基因修饰的骨髓间充质干细胞对缺氧复氧神经细胞的保护作用。
方法：胶质源性神经营养因子基因修饰的骨髓间充质干细胞经诱导后与缺氧复氧神经细胞共培养，采用Hoechst33258和细胞免疫荧光染色分别检测神经细胞凋亡及生长相关蛋白43在共培养细胞中的表达。
结果与结论：骨髓间充质干细胞/胶质源性神经营养因子组的神经细胞凋亡率显著低于骨髓间充质干细胞组和缺氧组(P < 0.05)，骨髓间充质干细胞/胶质源性神经营养因子组表达生长相关蛋白43的吸光度值明显高于骨髓间充质干细胞组(P < 0.05)。提示骨髓间充质干细胞/胶质源性神经营养因子通过抑制缺氧复氧神经细胞凋亡和促进共培养细胞生长相关蛋白43表达发挥神经保护作用。

关键词: 胶质源性神经营养因子, 骨髓间充质干细胞, 缺氧复氧, 神经细胞, 共培养

Abstract:

BACKGROUND: Preliminary studies in workgroup indicated that glial-derived neurotrophic factor (GDNF) gene modified by bone marrow mesenchymal stem cells (BMSCs) (BMSCs/GDNF) can stably express the mRNA of GDNF, but whether GDNF protein in BMSCs/GDNF affects neurons subjected to anoxia-reoxygenation positively is unclear.
OBJECTIVE: To observe the protective effects of BMSCs/GDNF on neurons subjected to anoxia-reoxygenation.
METHODS: BMSCs/GDNF were co-cultured with anoxia-reoxygenation nerve cells. Hoechst33258 staining was used to detect apoptosis of neurons. Immunofluorescent-chemistry method was used to detect growth-associated protein-43 (GAP-43) expression in co-cultured cells.
RESULTS AND CONCLUSION: The rate of neurons apoptosis in the BMSCs/GDNF group was lower than that of the BMSCs group and anoxia group (P < 0.05). Absorbance of growth-associated protein-43 (GAP-43) expression in the BMSCs/GDNF group was higher than that in the BMSCs group (P < 0.05). It is indicated that BMSCs/GDNF have neuroprotective effects due to inhibiting apoptosis of neurons subjected to anoxia-reoxygenation and increasing GAP-43 expression in co-cultured cells.

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