中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (47): 8782-8786.doi: 10.3969/j.issn.1673-8225.2011.47.011

• 复合支架材料 composite scaffold materials • 上一篇    下一篇

聚己内酯/丝胶蛋白静电混纺膜炎症响应的体内评价

吕永钢1,2，林崇文1,2，李林昊1,2，钱宇娜1,2，张小梅1,2，杨  力1,2，何建华1,2   

  1. 1“生物流变科学与技术”教育部重点实验室(重庆大学)，重庆大学生物工程学院，重庆市  400044   
    2重庆大学，生物工程学院国家“111计划”基地，重庆市  400044
  • 收稿日期:2011-08-03 修回日期:2011-10-20 出版日期:2011-11-19 发布日期:2011-11-19
  • 作者简介:吕永钢☆，男，1977年生，湖北省黄梅县人，汉族，2005年中国科学院理化技术研究所毕业，博士，教授，主要从事生物力学与组织修复研究。 yglv@cqu.edu.cn
  • 基金资助:

    新世纪优秀人才支持计划资助项目(NCET-10-0879)；重庆市科委科技攻关重大项目资助项目(2009AA5045)；国家自然科学基金资助项目(11172338)；教育部留学回国人员科研启动基金资助项目；重庆大学大型仪器设备开放基金资助项目。

Inflammatory response evaluation for electrospun polycaprolactone/silk sericin membranes in vivo

Lü Yong-gang1,2, Lin Chong-wen1,2, Li Lin-hao1,2, Qian Yu-na1,2, Zhang Xiao-mei1,2, Yang Li1,2, He Jian-hua1,2   

  1. 1Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, Bioengineering College, Chongqing University, Chongqing  400044, China
    2“111” Project Laboratory of Biomechanics and Tissue Repair, Bioengineering College, Chongqing University, Chongqing  400044, China
  • Received:2011-08-03 Revised:2011-10-20 Online:2011-11-19 Published:2011-11-19
  • About author:Lü Yong-gang☆, Doctor, Professor, Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, Bioengineering College, Chongqing University, Chongqing 400044, China; “111” Project Laboratory of Biomechanics and Tissue Repair, Bioengineering College, Chongqing University, Chongqing 400044, China yglv@cqu.edu.cn
  • Supported by:

    the Program for New Century Excellent Talents in University, No. NCET-10-0879*; the Key Science and Technology Program of Chongqing Science and Technology Commission, No. 2009AA5045*; the National Natural Science Foundation of China, No. 11172338*; the Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry*; the Sharing Fund of Chongqing University's Large-scale Equipment* 

PDF

381

摘要：

背景：研究表明，丝胶蛋白可以增加哺乳动物细胞的增殖，促进伤口愈合，具有良好的抗菌、抗氧化、抗癌的活性，同时具有良好的生物相容性和可降解性，是一种理想的组织工程材料。然而目前对丝胶蛋白能否引起炎症响应仍存在争论。
目的：评价聚己内酯/丝胶蛋白静电混纺膜的炎症响应。
方法：将不同混合比例的聚己内酯/丝胶蛋白纳米纤维薄膜体内埋植入大鼠背部肌肉，4周后取出进行组织学观察，通过苏木精-伊红染色和巨噬细胞抗体CD68免疫组织化学染色评价其炎症响应。
结果与结论：聚己内酯/丝胶蛋白7∶3组混纺膜炎症反应与丝素蛋白膜类似，小于聚己内酯/丝胶蛋白6∶4组、聚己内酯/丝胶蛋白5∶5组和聚己内酯组；聚己内酯/丝胶蛋白6∶4组与聚己内酯/丝胶蛋白5∶5组与聚己内酯组类似。提示聚己内酯/丝胶蛋白静电混纺膜不会引起显著的炎症响应，少量添加丝胶蛋白可以降低聚己内酯的炎症反应，随着丝胶蛋白含量的增加，炎症反应呈上升趋势。

关键词: 丝胶蛋白, 炎症响应, 组织工程, 体内, 免疫组织化学

Abstract:

BACKGROUND: Studies have shown that silk sericin (SS) can accelerate the proliferation of mammalian cells and promote wound healing. SS has good activities for antibacteria, antioxidation and anticancer. SS also has a good biocompatibility and biodegradability. It is an ideal material for tissue engineering, however, there is still a controversy of whether SS causes inflammatory response so far.
OBJECTIVE: To evaluate the inflammatory response of electrospun polycaprolactone (PCL)/SS membranes.
METODS: Materials with different PCL/SS ratios were implanted into the back muscles of rats and were taken out after 4 weeks. The inflammatory response was evaluated by hematoxylin eosin staining and macrophage antibody CD68 immunohistochemistry.
RESULTS AND CONCLUSION: The inflammatory response of the 7:3 group of PCL/SS membranes is similar to that of silk fibroin membrane and was less than those of the 6:4, 5:5 and PCL groups. The 6:4 and 5:5 groups have similar inflammatory response with PCL group. Electrospun PCL/SS membranes can not cause significant inflammatory response. A little amount of SS can decrease the inflammatory response of PCL membrane. However, the inflammatory response of PCL/SS membrane can increase as the ratio of SS to PCL increased.

中图分类号: