中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (47): 8741-8744.doi: 10.3969/j.issn.1673-8225.2011.47.001

• 组织工程骨及软骨材料 tissue-engineered bone and cartilage materials •    下一篇

Choukroun富血小板纤维蛋白复合自体微小颗粒骨修复兔颅骨缺损

王得利1，孔祥盼2，栾海蓉1，芦  山1，商维荣3   

  1. 1牡丹江医学院，黑龙江省牡丹江市  157011
    2黑龙江省牡丹江红旗医院口腔科，黑龙江省牡丹江市157011
    3吉林省长春高新技术开发区社会事业发展局社会卫生服务中心，吉林省长春市   130021
  • 收稿日期:2011-04-02 修回日期:2011-05-09 出版日期:2011-11-19 发布日期:2011-11-19
  • 通讯作者: 商维荣，教授，长春高新技术开发区社会事业发展局社会卫生服务中心，吉林省长春市 130021 koujieswr@ 163.com
  • 作者简介:王得利★，男，1979年生，黑龙江省牡丹江市人，汉族，佳木斯大学在读硕士，讲师，主要从事口腔基础医学研究工作。
  • 基金资助:

    黑龙江省卫生厅科研课题(2010-255)，课题名称：Choukroun富血小板纤维蛋白联合自固化型磷酸钙人工骨(CPC)修复兔颅骨骨缺损的实验研究；牡丹江医学院科学技术研究项目(2010-29)，课题名称：Choukroun富血小板纤维蛋白用于牙周组织再生的研究。

Choukroun’s platelet-rich fibrin combined with autogenic morselized bone promotes rabbit skull regeneration

Wang De-li1, Kong Xiang-pan2, Luan Hai-rong1, Lu Shan1, Shang Wei-rong3   

  1. 1Mudanjiang Medical College, Mudanjiang  157011, Heilongjiang Province, China
    2Department of Stomatology, Hongqi Hospital, Mudanjiang  157011, Heilongjiang Province, China
    3Social Medical Care Center, Office of Social Enterprise Development, Development Area of Changchun, Changchun  130021, Jilin Province, China
  • Received:2011-04-02 Revised:2011-05-09 Online:2011-11-19 Published:2011-11-19
  • Contact: Shang Wei-rong, Professor, Social Medical Care Center, Office of Social Enterprise Development, Development Area of Changchun, Changchun 130021, Jilin Province, China koujieswr@163.com
  • About author:Wang De-li★, Studying for master’s degree, Lecturer, Mudanjiang Medical College, Mudanjiang 157011, Heilongjiang Province, China
  • Supported by:

    the Scientific Research Project of Heilongjiang Health Bureau, No. 2010-255*; Scientific Research Project of Mudanjiang Medical College, No. 2010-29*

PDF

516

被引次数

9

摘要：

背景：Choukroun富血小板纤维蛋白与自体微小颗粒骨具有促进骨缺损修复的能力，但单独应用均有其不足，拟将两种材料结合使用以期达到理想效果。
目的：观察Choukroun富血小板纤维蛋白复合自体微小颗粒骨修复颅骨缺损的效果。
方法：采用自体对照方法在大耳白兔颅骨中缝两侧利用磨骨术各制备标准一致的骨缺损，即刻于一侧骨缺损处回填Choukroun富血小板纤维蛋白膜与自体微小颗粒骨混合物作为实验组，另一侧只填充Choukroun富血小板纤维蛋白膜作为对照组，术后4，6，8周行软X射线摄片与组织病理切片观察。
结果与结论：实验组骨小梁面积显著大于对照组，骨愈合程度均优于对照组，骨小梁密度、形态、新生骨质的量及结构、成骨细胞数量均优于对照组(P < 0.05或P < 0.01)，说明Choukroun富血小板纤维蛋白与自体微小颗粒骨复合材料较Choukroun富血小板纤维蛋白具有更明显促进骨缺损修复的作用。

关键词: 自体微小颗粒骨, Choukroun富血小板纤维蛋白, 骨再生, 骨缺损, 修复

Abstract:

BACKGROUND: Autogenic morselized bone is an ideal substitute for tissue engineered stent. Choukroun’s platelet-rich fibrin (PRF) can enhance the bone regeneration. Whether the combination of the two materials can achieve a better effect need to be explored.
OBJECTIVE: To investigate the effect of Choukroun’s PRF combined with autogenic morselized bone in repairing skull bone defects.
METHODS: Two standard defects were made by grinded osteotomy on each animal’s skull and then Choukroun’s PRF combined with autogenic morselized bone were used to filled the defect sites on one side as the experimental group and Choukroun’s PRF alone was used on the another side as control group. At postoperatively 4, 6, and 8 weeks, soft X-ray and histological staining were applied.
RESULTS AND CONCLUSION: The new bone area was significantly larger in the experimental group than in the control group as well as bone healing, bone trabacula density and shape, volume and structure of new bone and number of osteoblasts (P < 0.05 or P < 0.01). It is indicated that Choukroun’s PRF combined with autogenic morselized bone can significantly improve the repair of bone defects superior to Choukroun’s PRF alone.

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