中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (45): 8491-8494.doi: 10.3969/j.issn.1673-8225.2011.45.029

• 干细胞基础实验 basic experiments of stem cells • 上一篇    下一篇

急性髓系白血病患者外周血单个核细胞Toll样受体9以及血清肿瘤坏死因子和凋亡基因FAS的表达

邱元秀，莫东华，王晓桃   

  1. 桂林医学院附属医院血液内科，广西壮族自治区桂林市  541001
  • 收稿日期:2011-05-07 修回日期:2011-06-11 出版日期:2011-11-05 发布日期:2011-11-05
  • 通讯作者: 莫东华，硕士生导师，教授，桂林医学院附属医院血液内科，广西壮族自治区桂林市541001 tonyqyx@163.com
  • 作者简介:邱元秀★，女，1982年生，河南省郑州市人，桂林医学院在读硕士，主要从事血液系统恶性肿瘤方面的研究。 tonyqyx@163.com
  • 基金资助:

    广西卫生厅资助课题(Z2010274，Z2011200)。

Detection of Toll-like receptor 9 in peripheral blood mononuclear cells and serum tumor necrosis factor and Fas in patients with acute myeloid leukemia

Qiu Yuan-xiu, Mo Dong-hua, Wang Xiao-tao   

  1. Department of Hematology, Hospital Affiliated to Guilin Medical University, Guilin  541001, Guangxi Zhuang Autonomous Region, China
  • Received:2011-05-07 Revised:2011-06-11 Online:2011-11-05 Published:2011-11-05
  • Contact: Mo Dong-hua, Master’s supervisor, Associate professor, Department of Hematology, Hospital Affiliated to Guilin Medical University, Guilin 541001, Guangxi Zhuang Autonomous Region, China tonyqyx@163.com
  • About author:Qiu Yuan-xiu★, Studying for master’s degree, Department of Hematology, Hospital Affiliated to Guilin Medical University, Guilin 541001, Guangxi Zhuang Autonomous Region, China tonyqyx@163.com
  • Supported by:

    the Plan Program of Health Department of Guangxi Zhuang Autonomous Region, No.Z2010274, Z2011200**

PDF

279

被引次数

3

摘要：

背景：Toll样受体9(TLR9)、肿瘤坏死因子a、Fas可能共同参与白血病的发生发展过程。
目的：测定TLR9在急性髓系白血病患者外周血单个核细胞中的表达及血清肿瘤坏死因子α、Fas水平。
方法：从急性髓系白血病患者与正常对照组中分离出外周血单个核细胞，采用反转录-聚合酶链反应法检测外周血单个核细胞中TLR9 mRNA的表达水平，采用酶联免疫吸附试验法检测血清肿瘤坏死因子α、Fas水平。
结果与结论：在急性髓系白血病患者初治组和难治复发组中，外周血单个核细胞中TLR9 mRNA表达高于正常对照组(P < 0.01)，化疗后完全缓解组与正常对照组差异无显著性意义(P > 0.05)；各病例组血清肿瘤坏死因子α、Fas水平显著高于正常对照组(P < 0.01)。TLR9 mRNA的表达与血清肿瘤坏死因子α、Fas水平均呈正相关。

关键词: 急性髓系白血病, Toll样受体9, 外周血单个核细胞, 肿瘤坏死因子, 凋亡相关基因

Abstract:

BACKGROUND: Toll-like receptor 9 (TLR9), tumor necrosis factor alpha (TNF-α) and Fas may together participate in the occurrence and development of acute myeloid leukemia (AML).
OBJECTIVE: To study the expression of TLR9 in peripheral blood mononuclear cells (PBMNCs), levels of TNF-α and apoptosis-related genes in serum of patients with acute myeloid leukemia (AML).
METHODS: The expression of TLR9 mRNA in PBMNCs of AML patients and normal control group was detected by reverse transcription-polymerase chain reaction (RT-PCR), the serum levels of TNF-α and Fas were detected were by enzyme-linked immunosorbent assay in each group.
RESULTS AND CONCLUSION: Compared to normal control group, the expression of TLR9 mRNA in PBMNCs of in AML patients with untreated and refractory/relapsed groups was significantly increased (P < 0.01), and complete remission group was not statistically significant (P > 0.05). The serum levels of TNF-α and Fas in AML patients were significantly higher than those in the normal control group (P > 0.01). The expression of TLR9 was positively correlated with the serum levels of TNF- α and Fas.

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