中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (45): 8463-8468.doi: 10.3969/j.issn.1673-8225.2011.45.023

• 干细胞移植 stem cell transplantation • 上一篇    下一篇

骨髓间充质干细胞静脉移植治疗大鼠脊髓损伤

于长申1，管雅琳2，王世民2，徐小林2，王新平2，阎晓玲3，张雪青4   

  1. 1天津医科大学研究生院，天津市300070；天津市环湖医院，2神经内科，3病理科，4神经电生理科，天津市  300060
  • 收稿日期:2011-08-04 修回日期:2011-09-14 出版日期:2011-11-05 发布日期:2011-11-05
  • 通讯作者: 王世民，教授，硕士生导师，天津市环湖医院神经内科，天津市 300060 wangxinpingtj@sina.com
  • 作者简介:于长申★，男，1981年生，天津市宁河县人，汉族，天津医科大学在读硕士，主要从事神经危重症方面的研究。 Yalin82@qq.com
  • 基金资助:

    天津市卫生局科技基金支持项目(05KY17),课题名称：骨髓间充质干细胞移植治疗大鼠脊髓损伤的实验研究。

Intravenous transplantation of bone marrow mesenchymal stem cells in the treatment of spinal cord injury in rats

Yu Chang-shen1,Guan Ya-lin2, Wang Shi-min2, Xu Xiao-lin2, Wang Xin-ping2, Yan Xiao-ling3, Zhang Xue-qing4   

  1. 1Postgraduate College, Tianjin Medical University, Tianjin  300070, China; 2Department of Neurology, 3Department of Pathology, 4Department of Neurophysiology, Huanhu Hospital of Tianjin, Tianjin  300060, China
  • Received:2011-08-04 Revised:2011-09-14 Online:2011-11-05 Published:2011-11-05
  • Contact: Wang Shi-min, Professor, Master’s supervisor, Department of Neurology, Huanhu Hospital of Tianjin, Tianjin 300060, China wangxinpingtj@sina.com
  • About author:Yu Chang-shen★, Studying for master’s degree, Postgraduate College, Tianjin Medical University, Tianjin 300070, China Yalin82@qq.com
  • Supported by:

    the Science and Technology Foundation of Tianjin Health Bureau, No. 05KY17*

PDF

279

被引次数

3

摘要：

背景：干细胞移植可以重建中枢神经系统的结构和功能，近年来引起了广泛的关注。
目的：尾静脉移植骨髓间充质干细胞观察其对大鼠脊髓损伤修复的影响并探讨其机制。
方法：密度梯度离心法结合贴壁筛选法分离骨髓间充质干细胞。将成年雌性Wistar大鼠以动脉瘤夹钳夹法制备大鼠脊髓损伤模型后随机分为细胞移植组和对照组，分别干预。
结果与结论：细胞移植组大鼠BBB后肢功能评分恢复优于对照组(P < 0.05)。自损伤后第30天开始，细胞移植组大鼠运动诱发电位潜伏期及体感诱发电位P1波潜伏期的恢复均优于对照组(P < 0.05)，并持续至实验结束。细胞移植组大鼠脊髓内在损伤中心及头、尾端距离脊髓损伤中心1 cm处均可见阳性细胞。说明尾静脉注射移植骨髓间充质干细胞可以促进脊髓损伤大鼠神经功能恢复，其机制可能与移植细胞迁移到损伤部位，分化为神经元样和神经胶质细胞样细胞，并分泌或促进宿主分泌神经营养因子有关。

关键词: 脊髓损伤, 骨髓间充质干细胞, 静脉移植, 细胞移植, 神经功能

Abstract:

BACKGROUND: Transplantation of stem cells can rebuild the structure and function of injured central nervous system, and has attracted wide attention in recent years.
OBJECTIVE: To explore the effects and mechanisms of bone marrow mesenchymal stem cells (BMSCs) transplanted through the vena caudalis on neurological recovery of rats with spinal cord injury.
METHODS: BMSCs were separated with density gradient centrifugation and adherent screening methods. Forty adult female Wistar rats were used to establish the models of spinal cord injury using aneurysm clips, and then randomized into two groups: control group and BMSCs transplantation group.
RESULTS AND CONCLUSION: The BMSCs transplantation group showed higher BBB scores than the control group (P < 0.05). Since the 30th day after injury, the motor evoked potential latency and somatosensory evoked potential P1 wave latency of the BMSCs transplantation group were shorter than those of the control group (P < 0.05), and these phenomena continued until the end of experiment. Positive cells could be noted at the sites 1 cm distant from the injured centre of the spinal cord. BMSCs transplantation via the vena caudalis can remarkably promote the recovery of neural function in rats with spinal cord injury, and the mechanism may be related to that transplanted BMSCs can survive, migrate and differentiate into neuron like cells and neuroglia like cells, and can promote the expression of neurotrophic factors.

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