中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (36): 6691-6695.doi: 10.3969/j.issn.1673-8225.2011.36.010

• 骨髓干细胞 bone marrow stem cells • 上一篇    下一篇

流式细胞仪观察脉冲电磁场干预骨髓间充质干细胞的生长

黄  钊，苏  伟，崔向荣，覃万安   

  1. 广西医科大学第一附属医院创伤骨科手外科，广西壮族自治区南宁市  530021
  • 收稿日期:2011-06-24 修回日期:2011-08-09 出版日期:2011-09-03 发布日期:2011-09-03
  • 通讯作者: 苏伟，教授，主任医师，广西医科大学第一附属医院创伤骨科手外科，广西壮族自治区南宁市 530021 gxsuwei@163.com
  • 作者简介:黄钊★，男，1983年生，广西壮族自治区南宁市人，壮族，广西医科大学在读硕士，主要从事电磁场干预骨髓间充质干细胞的基因和蛋白组学研究。 gxmu@stemcell8.com
  • 基金资助:

    国家自然科学基金项目(30860078)、广西壮族自治区教育厅科研项目(200710MS021)及广西医科大学第一附属医院科研基金项目。

Flow-cytometric method for observing the effects of pulsed electromagnetic fields on the growth of rat bone marrow mesenchymal stem cells

Huang Zhao, Su Wei, Cui Xiang-rong, Qin Wan-an   

  1. Department of Trauma Osteology and Hand Surgery, First Affiliated Hospital of Guangxi Medical University, Nanning  530021, Guangxi Zhuang Autonomous Region, China
  • Received:2011-06-24 Revised:2011-08-09 Online:2011-09-03 Published:2011-09-03
  • Contact: Su Wei, Professor, Chief physician, Department of Trauma Osteology and Hand Surgery, First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China gxsuwei@163.com
  • About author:Huang Zhao★, Studying for master’s degree, Department of Trauma Osteology and Hand Surgery, First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China gxmu@stemcell8.com
  • Supported by:

    the National Natural Science Foundation of China, No. 30860078*; Scientific Research Program of Education Department of Guangxi Zhuang Autonomous Region, No. 200710MS021*; Scientific Research Fund of the First Affiliated Hospital of Guangxi Medical University*

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被引次数

1

摘要：

背景：应用流式细胞仪检测细胞表型、存活或凋亡细胞计数及细胞周期，较形态学观察、DNA电泳等检测方法更具优势。
目的：采用流式细胞仪分选检测特定脉冲电磁场干预对大鼠骨髓间充质干细胞生长周期及其增殖效率的影响。
方法：使用振荡频率1 kHz，磁感应强度0.05 mT、功率密度5 mW/cm2的脉冲电磁场照射大鼠骨髓间充质干细胞，以未经磁场干预的细胞为对照。采用流式细胞仪检测未经磁场干预细胞表面抗原CD29、CD31、CD44、CD45和CD105表达率；于传第3代后第3，6，9，12天测定不同干预细胞增殖率及生长周期。
结果与结论：未经磁场干预的第3代大鼠骨髓间充质干细胞表面抗原CD29、CD44和CD45为阳性表达，CD31和CD105为阴性表达，较未经磁场干预的第2代细胞更纯化(P < 0.05)；经磁场干预后第3代细胞存活率及细胞周期S期百分比较未经磁场干预的第3代细胞高(P < 0.05)。流式细胞仪检测证实特定频率和场强的脉冲电磁场能在一定程度上促进骨髓间充质干细胞的生长与增殖。

关键词: 脉冲电磁场, 骨髓间充质干细胞, 流式细胞仪, 细胞周期, 存活率

Abstract:

BACKGROUND: Compared with the morphology, DNA electrophoresis and other methods, flow-cytometric method has more advantages on the detection of cell phenotype, proliferation rate and cell cycle of rat bone marrow mesenchymal stem cells (BMSCs).
OBJECTIVE: To observe and discuss the effect of specific pulsed electromagnetic fields stimulation on the proliferation and cell cycle of rat BMSCs with the flow-cytometric method (FCM).
METHODS: Rat BMSCs were exposed to pulsed electromagnetic fields (frequency for 1 kHz, magnetic flux density for 0.05 mT, power density for 5 mW/cm2). BMSCs without exposure to pulsed electromagnetic fields were used as controls. Expression of CD29, CD31, CD44 CD45, CD105 were detect in the control group. The proliferation rate and cell cycle of passage 3 cells were detected at 3, 6, 9, 12 days.
RESULTS AND CONCLUSION: CD29, CD44, CD105 in the 3rd passage non-stimulated BMSCs was positively expressed and the CD31, CD45 was negatively expressed (P < 0.05). The survival rate of passage 3 BMSCs and percentage of S phase following intervention of pulsed electromagnetic fields were greater than those in the control group (P < 0.05). These results indicated that the specific pulsed electromagnetic fields can promote the growth and proliferation of BMSCs to some extent.

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