中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (16): 2915-2919.doi: 10.3969/j.issn.1673-8225.2011.16.015

• 纳米生物材料 nanobiomaterials • 上一篇    下一篇

纳米银抗菌医用敷料创面外用后纳米银在体内的分布

耿  健1，柳大烈1，张  阳2，奚廷斐3，张志雄3，蹇明丽4，黄建林4，朱小玲4，曾孔仙4   

  1. 1南方医科大学珠江医院整形外科，广东省广州市  510282
    2解放军四五八医院烧伤整形显微外科，广东省广州市  510602
    3深港产学研基地生物医学工程研究中心，广东省深圳市 518057
    4深圳市源兴纳米医药科技有限公司，广东省深圳市518057   
  • 收稿日期:2010-08-19 修回日期:2010-09-29 出版日期:2011-04-16 发布日期:2013-11-11
  • 作者简介:耿健★，男，1984年生，江苏省泰兴市人，汉族，南方医科大学整形外科在读硕士，医师，主要从事整形外科研究。 gengjian144@163.com

Nano sliver distribution in vivo after the application of silver nanoparticle dressings

Geng Jian1, Liu Da-lie1, Zhang Yang2, Xi Ting-fei3, Zhang Zhi-xiong3, Jian Ming-li4, Huang Jian-lin4, Zhu Xiao-ling4, Zeng Kong-xian4   

  1. 1Department of Plastic and Cosmetic Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou  510282, Guangdong Province, China
    2Department of Burns and Plastic Microsurgery, The 458 hospital of Chinese PLA, Guangzhou  510602, Guangdong Province, China
    3PKU-HKUST Shenzhen-Hongkong Institution, Shenzhen  518057, Guangdong Province, China
    4Shenzhen Tsinghua Yuanxing Nano- pharmaceutical Co., LTD., Shenzhen  518057, Guangdong Province, China
  • Received:2010-08-19 Revised:2010-09-29 Online:2011-04-16 Published:2013-11-11
  • About author:Geng Jian★, Studying for master’s degree, Physician, Department of Plastic and Cosmetic Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, Guangdong Province, China gengjian144@163.com

PDF

481

被引次数

50

摘要：

背景：纳米银以小尺寸效应、量子效应和极大的比表面积优势，较传统银制品抗菌效果更为突出，但有关其进入体内后产生的负面生物效应或不良反应尚不明确。
目的：观察创面外用纳米银敷料后纳米银在兔体内的分布变化。 
方法：将新西兰白兔以抽签法随机分为正常对照组(创面自然愈合)、单次敷料组(又分为6小组)、多次敷料组(分为6小组)。均于兔双耳腹侧制作创面，单次敷料组单次使用纳米银敷料外敷，不更换敷料；多次敷料组多次使用纳米银敷料外敷，其6小组分别于0 d，2 d，2 d和4 d，2 d和4 d，2 d和4 d，2 d和4 d更换敷料。于创面制作后2，4，7，14，30，60 d检测兔血、肝、肾、脾中银浓度。
结果与结论：应用纳米银敷料2d后，单次敷料组、多次敷料组血液、肝、肾、脾中银浓度均明显高于正常对照组(P < 0.01)。应用第4天，单次敷料组、多次敷料组各脏器银浓度仍明显高于正常对照组(P < 0.01)，但多次敷料组各脏器银浓度随时间呈进行性上升趋势，而单次敷料组各脏器银浓度呈进行性下降，这种变化在第7天时更为明显，多次敷料组各脏器银浓度显著高于单次敷料组(P < 0.01)。移除纳米银敷料后，单次敷料组、多次敷料组各脏器银含量均较前一时间点银浓度显著降低，尤以多次敷料组更为明显(P < 0.01)。制作创面后第30，60天，单次敷料组、多次敷料组各脏器银浓度与正常对照组已无差异(P > 0.05)。说明纳米银敷料用于创伤创面时能迅速入血，通过血液循环分布于肝、肾、脾等脏器；移除敷料后，血、肝、肾、脾中纳米银含量迅速下降至正常值，不会产生累积现象。

关键词: 银, 创伤, 纳米颗粒, 敷料, 分布, 生物材料与纳米技术

Abstract:

BACKGROUND: Silver nanoparticles are greatly superior to traditional sliver products in terms of antibacterial effect due to small size effect, quantum effect and large specific surface area. The negative biological effect or adverse reaction in vivo remains unclear.
OBJECTIVE: To investigate the nano-sliver distribution in rabbits after the application of silver nanoparticle dressings on trauma. 
METHODS: New Zealand white rabbits were randomly divided into three groups: normal control group (trauma wounds healed naturally), dressings for single time group and dressings for multiple times group. Both dressing groups were respectively assigned into six subgroups. Full-thickness excisional wounds were created over the ventral surface of rabbits’ ears. Rabbits in dressings for single time group were treated with silver nanoparticle dressings for single time and in dressings for multiple times group were treated with dressings for multiple times. Dressings in six subgroups of dressings for multiple times were changed respectively in 0, 2, 2 and 4, 2 and 4, 2 and 4, 2 and 4 post treatment days after injury. Blood, liver, kidney and spleen were respectively collected to determine the silver contents in 2, 4, 7, 14, 30, 60 days after injury.
RESULTS AND CONCLUSION: The sliver contents in blood, liver, kidney and spleen of dressing groups at 2 days were significantly higher than those of normal control group (P < 0.01). The sliver contents in blood, liver, kidney and spleen of dressing groups at 4 days and were significantly higher than those of normal control group (P < 0.01). The contents in multiple dressing group were progressively increasing with time went, while the contents in single dressing group was progressively decreasing. This alteration was more obvious at 7 days, with significant differences between two dressing groups (P < 0.01). After removing the dressings, the sliver contents of dressing groups significantly declined compared with previous time point, especially in multiple dressing group (P< 0.01). At 30 and 60 days post injury, there was no significant difference in the sliver contents in blood, liver, kidney and spleen between dressing groups and normal control group (P > 0.05). After the application of silver nanoparticle dressings on trauma, it is rapidly absorbed through skin wounds into the circulatory system and then deposited into the liver, kidney and spleen. While removing the dressings, the sliver contents in blood, liver, kidney and spleen declined rapidly to the normal value without any accumulation.

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