中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (15): 2817-2820.doi: 10.3969/j.issn.1673-8225.2011.15.038

• 组织构建学术探讨 tissue construction academic discussion • 上一篇    下一篇

与应力调控相关的基因选择性剪接

傅  亚1, 2, 3，张徭尧1, 2，孙姣霞1, 2，向  燕1, 2，王远亮1, 2   

  1. 重庆大学，1生物流变科学与技术教育部重点实验室，2生物工程学院生物材料与仿生工程研究中心，重庆市 400044；3重庆科技学院生物系，重庆市  411331
  • 收稿日期:2010-12-11 修回日期:2011-01-18 出版日期:2011-04-09 发布日期:2013-11-06
  • 通讯作者: 王远亮，博士，教授，重庆大学，生物流变科学与技术教育部重点实验室，生物工程学院生物材料与仿生工程研究中心，重庆市 400044 bio730@cqu.edu.cn
  • 作者简介:傅亚☆，女，1970年生，四川省大邑县人，汉族，重庆大学在读博士，副教授，主要从事组织工程与生物材料方面的研究。 cqfuya@163. com
  • 基金资助:

    国家自然科学基金(30870609，51043004)；重庆市自然科学基金(CSTC2009BB4382)；重庆市教委科学技术研究项目(Kj091415)；九龙坡区科技计划项目(高090109)。

Gene alternative splicing related to stress regulation

Fu Ya1, 2, 3, Zhang Yao-yao1, 2, Sun Jiao-Xia1, 2, Xiang Yan1, 2, Wang Yuan-liang1, 2   

  1. 1Key Laboratory of Biorheological Science and Technology, Ministry of Education, 2Research Center of Bioinspired Materials Science and Engineering, Bioengineering College, Chongqing University, Chongqing  400044, China; 3Department of Biology, Chongqing University of Science and Technology, Chongqing  401331, China
  • Received:2010-12-11 Revised:2011-01-18 Online:2011-04-09 Published:2013-11-06
  • Contact: Wang Yuan-liang, Doctor, Professor, 1Key Laboratory of Biorheological Science and Technology, Ministry of Education, 2Research Center of Bioinspired Materials Science and Engineering, Bioengineering College, Chongqing University, Chongqing 400044, China bio730@cqu.edu.cn
  • About author:Fu Ya☆, Studying for doctorate, Associate professor, 1Key Laboratory of Biorheological Science and Technology, Ministry of Education, 2Research Center of Bioinspired Materials Science and Engineering, Bioengineering College, Chongqing University, Chongqing 400044, China; Department of Biology, Chongqing University of Science and Technology, Chongqing 401331, China cqfuya@163.com
  • Supported by:

    the National Natural Science Foundation of China, No. 30870609*, 51043004*; Natural Science Foundation of Chongqing, No. CSTC2009BB4382*; Foundation of Chongqing Municipal Education Commission, No. Kj091415*; the Science and Technology Plan of Jiulongpo Area, No. 090109*

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摘要：

背景：适当的力学环境是生物体正常生长发育、结构重建以及功能维持的重要因素，也是损伤组织功能性修复的关键因素之一。应力调控基因表达不仅体现在基因的开关或表达水平的调节，还可以体现在转录后的选择性剪接。
目的：结合力生长因子介绍选择性剪接这种新的应力调控方式，并推测可能的调控机制。
方法：检索PubMed 数据库(1964/2010)、CNKI数据库(2000/2009)有关力信号转导和基因选择性剪接方面的综述文章和研究报告，分析二者的联系和可能的调控机制。
结果与结论：应力刺激可以导致肌细胞、成骨细胞中的胰岛素样生长因子1基因发生选择性剪接，产生一种新的应力敏感的生长因子力生长因子，这种新型调控方式的机制还不明确，推测与应力导致的剪接小体的位置(位移运动)以及改变了剪接酶(如RNP酶)的位置和空间结构(变形)有关。

关键词: 选择性剪接, 生物力学, 力学生物学, 力生长因子, 组织修复

Abstract:

BACKGROUND: The proper mechanical environment is not only the important factors for organism normal growth and development, structure reestablishment and function maintenance, but also is one of the key factors for functional recovery of injured tissue. The regulation of gene expression by stress does not only reflect the switch or regulation of gene expression, but also is related to alternative splicing after transcription.
OBJECTIVE: The introduction of a new stress regulation by alternative splicing combined with growth factor binding, and to predict the presumable regulatory mechanism.
METHODS: PubMed database (1964 to 2010) and CNKI database (2000 to 2009) were searched for relative review articles and research reports for aspects of signal transduction and genetic alternative splicing, the linkages between the two and possible regulatory mechanisms were analyzed.
RESULTS AND CONCLUSION: Stress stimulation could lead to alternative splicing of insulin like growth factor-1 genes in muscle cells and bone cells, which would produce a new stress-sensitive growth factor mechano-growth factor and reveal a new regulation by stress. The mechanism of regulation is not clear, which may be related to the stress caused by the location of splicing bodies (the displacing movement) and the change of the location and spatial structure (deformation) of splicing enzymes (such as RNP enzyme).

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