中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (14): 2535-2539.doi: 10.3969/j.issn.1673-8225.2011.14.015

• 干细胞因子及调控因子 stem cell factors and regulatory factors • 上一篇    下一篇

植物源性活性因子促进骨髓间充质干细胞向心肌细胞的分化

彭  鹏1，莫烨华1，林晓莉2，李中玉2，程  蕾2，周  震2，李  明1，2   

  1. 1暨南大学再生医学教育部重点实验室，广东省广州市  510632
    2香港创新医学实验室，香港 
  • 收稿日期:2010-12-14 修回日期:2011-01-28 出版日期:2011-04-02 发布日期:2013-11-02
  • 通讯作者: 李明，博士，教授，博士生导师，暨南大学再生医学教育部重点实验室，香港创新医学实验室，香港 li.ming@labinnovativemedicine.com
  • 作者简介:彭鹏★，女，1986年生，湖南省双峰县人，汉族，暨南大学生命科学技术学院在读硕士，主要从事缺血性心肌再生方向的研究。 xuanxuan86120@163.com
  • 基金资助:

    RGC grant (2140569) from Hong Kong Government Research Committee (470707)。

A natural activity factor promotes cardiogenic differentiation of bone marrow derived mesenchymal stem cells

Peng Peng1, Mo Ye-hua1, Lin Xiao-li2, Li Zhong-yu2, Cheng Lei2, Zhou Zhen2, Li Ming1,2   

  1. 1Key Laboratory for Regenerative Medicine, Ministry of Education, Jinan University, Guangzhou  510632, Guangdong Province, China
    2Laboratory of Innovative Medicine, Hong Kong Special Administrative Region, China
  • Received:2010-12-14 Revised:2011-01-28 Online:2011-04-02 Published:2013-11-02
  • Contact: Li Ming, Doctor, Professor, Doctoral supervisor, Key Laboratory for Regenerative Medicine, Ministry of Education, Jinan University, Guangzhou 510632, Guangdong Province, China; Laboratory of Innovative Medicine, Hong Kong Special Administrative Region, China li.ming@labinnovativemedicine.com
  • About author:Peng Peng★, Studying for master’s degree, Key Laboratory for Regenerative Medicine, Ministry of Education, Jinan University, Guangzhou 510632, Guangdong Province, China xuanxuan86120@163.com
  • Supported by:

    the RGC grant (2140569) from Hong Kong Government Research Committee No. 470707*

PDF

548

被引次数

6

摘要：

背景：以往研究已证实，在心肌梗死的大鼠体内，柔毛水杨梅粗提物EGJ能有效促进外源性的骨髓间充质干细胞分化成心肌细胞。课题组进一步分离EGJ，最终得到植物源性的活性因子cardiogenin，其是否也能促进间充质干细胞向心肌细胞分化呢？
目的：分离柔毛水杨梅的活性因子cardiogenin，并观察其促进骨髓间充质干细胞分化成心肌细胞的作用。
方法：分离并纯化大鼠的骨髓间充质干细胞，分别用柔毛水杨梅醇的粗提物EGJ 100 mg/L和cardiogenin 10 mg/L来处理间充质干细胞。在处理间充质干细胞的第3天和第7天用免疫荧光的方法进行心肌分化特异性标记物免疫染色，检测被处理的间充质干细胞是否表达心肌特异性标记物。
结果与结论：处理3 d后，EGJ组和cardiogenin组有39%的细胞表达心肌分化早期的特异性标记物 Mef2。第7天，EGJ组和cardiogenin组有70%以上的细胞表达心肌细胞的肌球蛋白重链 MHC。结果显示EGJ和cardiogenin均能诱导骨髓间充质干细胞向心肌细胞分化，但cardiogenin的用量比EGJ少5~10倍，诱导分化的效率也略高。

关键词: 柔毛水杨梅粗提物, 缺血性心脏病, 骨髓间充质干细胞, 活性因子, 心肌细胞, cardiogenin

Abstract:

BACKGROUND: Previous studies have demonstrated that the methanol extract of Geum japonicum (EGJ) can effectively promote the cardiogenic differentiation of the exogenous mesenchymal stem cells (MSCs), leading to substantial repair of infarcted heart with cardiac function significantly improved. In this study, the isolation of the active compound, which can induce cardiogenic differentiation of MSCs in cell culture system, was described through a bioassay guided fractionation strategy.
OBJECTIVE: To isolate active compound (cardiogenin) from Geum japonicum and to observe its activity in promoting cardiogenic differentiation of MSCs.
METHODS: The MSCs were isolated from the bone marrow of SD rats. The isolated MSCs were cultured and treated with EGJ (100 mg/L) and cardiogenin (10 mg/L) respectively. Immunofluorescent staining with antibodies specific for early cardiac differentiation marker (MEF2) and myosin heavy chain (MHC) was performed at 3 and 7 days after treatment respectively to monitor the process of cardiogenic differentiation of MSCs.
RESULTS AND CONCLUSION: It was found that approximately 39% of both EGJ and cardiogenin treated MSCs were positively stained with MEF2 at 3 days after treatment. On day seven of the treatment, more than 70% of both EGJ and cardiogenin treated MSCs were positively stained with MHC specific antibodies. The cardiogenin can significantly induce cardiogenic differentiation of MSCs in vitro as does EGJ, but the dose of cardiogenin required for induction of cardiopgenic differentiation of MSCs is much less but the induction efficiency is little higher than that of EGJ. 

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