中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (8): 1397-1401.doi: 10.3969/j.issn.1673-8225.2011.08.016

• 材料生物相容性 material biocompatibility • 上一篇    下一篇

二元镁合金在细胞培养基中的耐腐蚀能力及其生物相容性

杨春喜1,2，郑玉峰3，顾雪楠3，袁广银4，张  佳4，戴尅戎2   

  1. 1上海市第十人民医院(同济大学附属第十人民医院)骨科，上海市 200072
    2上海交通大学医学院附属第九人民医院骨科，上海市  200021
    3北京大学工学院先进材料与纳米技术系，北京市 100871
    4上海交通大学轻合金精密成型国家工程研究中心，上海市  200240
  • 收稿日期:2010-08-08 修回日期:2010-11-07 出版日期:2011-02-19 发布日期:2011-02-19
  • 通讯作者: 戴尅戎，工程院院士，主任医师，教授，上海交通大学医学院附属第九人民医院骨科，上海市 200021 krdai@163.com
  • 作者简介:杨春喜☆，男，1976年生，河北省秦皇岛市人，汉族，2010年上海交通大学医学院毕业，博士，主治医师，主要从事骨科，关节外科方面的研究。 chunxi_yang@163.com
  • 基金资助:

    上海市骨科内植物重点实验室(08DZ2230330)，上海市教委重点学科(J50206)，国家基础研究973计划(2007CB936101)，上海市启明星计划(08QA14045)，国家自然科学基金(30600628)。

Corrosion resistance and biocompatibility of binary magnesium alloys in cell culture medium

Yang Chun-xi1,2, Zheng Yu-feng3, Gu Xue-nan3, Yuan Guang-yin4, Zhang Jia4, Dai Ke-rong2   

  1. 1Department of Orthopedics, Shanghai Tenth People’s Hospital (Tenth People’s Hospital of Tongji University), Shanghai  200072, China
    2Department of Orthopedics, Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai  200011, China
    3Department of Advanced Materials and Nanotechnology, College of Engineering, Peking University, Beijing  100871, China
    4National Engineering Research Center of Light Alloys Net Forming, School of Materials Science and Engineering, Shanghai Jiao Tong University, Shanghai   200240, China
  • Received:2010-08-08 Revised:2010-11-07 Online:2011-02-19 Published:2011-02-19
  • Contact: Dai Ke-rong, Chief physician, Professor, Department of Orthopedics, Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China krdai@163.com
  • About author:Yang Chun-xi☆, Doctor, Attending physician, Department of Orthopedics, Shanghai Tenth People’s Hospital (Tenth People’s Hospital of Tongji University), Shanghai 200072, China; Department of Orthopedics, Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China chunxi_yang@163.com
  • Supported by:

    Shanghai Key Laboratory of Orthopedic Implants, No. 08DZ2230330*; Key Disciplines of Shanghai Municipal Education Commission, No. J50206*; the National Basic Research Program of China (973 Program), No. 2007CB936101*; Shanghai Rising-Star Program, No. 08QA14045*; the National Natural Science Foundation of China, No. 30600628*

PDF

406

被引次数

4

摘要：

背景：尽管已经有很多研究报道镁及其合金具有一定的骨传导性和可降解性，可以做为承重骨科内植物材料，但是还需要做更多的工作来正确评估它的潜力所在。
目的：检测镁和二元镁合金在细胞培养基中的降解特性和对成人骨髓间充质干细胞活性的影响。
方法：制备纯镁和8种二元镁合金(Mg-Al、Mg-Ca、Mg-Mn、Mg-Sn、Mg-Si、Mg-Y、Mg-Zn、Mg-Zr)样品；采用α-MEM细胞培养基(含体积分数为10%胎牛血清)模拟体内环境下制备标准浸提液，并检测pH值；电感耦合等离子体原子发射光谱仪检测浸提液中镁和添加元素含量；Alamar Blue法检测培养达1，3，5，7 d时100%、50%、25%浓度的纯镁和8种镁合金浸提液对成人骨髓间充质干细胞增殖的影响，判断８种二元镁合金的生物相容性。
结果与结论：Mg-Ca、Mg-Y合金耐腐蚀性较差，浸提液中Mg2+质量浓度分别达到(408.0±37.9) mg/L 和(351±15.3) mg/L，pH值分别为8.87±0.19和8.84±0.15；其次是Mg-Zr合金；其他二元合金耐腐蚀性与纯镁相当。结果表明纯镁和8种二元镁合金100%含量的浸提液均对成人骨髓间充质干细胞的增殖有显著抑制作用，当Mg2+质量浓度低于110 mg/L，pH值7.35~7.65时，纯镁和8种二元镁合金对成人骨髓间充质干细胞的增殖无显著影响。

关键词: 镁, 二元镁合金, 成人, 骨髓间充质干细胞, 生物相容性, 耐腐蚀性

Abstract:

BACKGROUND: Degrading magnesium and magnesium alloys are a new class of implant materials suitable for orthopedic surgery due to its bone conductibility and degradability, so further research on the evaluation of magnesium is very necessary.
OBJECTIVE: To determine the degradability of magnesium and binary magnesium alloys in cell culture medium and their influence on the viability of adult human bone marrow mesenchymal stem cells.
METHODS: Eight alloying elements Al, Ca, Mn, Si, Sn, Y, Zn and Zr were added into magnesium individually to fabricate binary Mg-1X (wt.%) alloys. Pure magnesium and eight binary magnesium alloys were immersed in α-MEM (containing 10% fetal bovine serum) to prepare extracts. The pH values of the extraction media were measured. Magnesium and alloy element ions in the extraction medium were analyzed using inductively coupled plasma-atomic emission spectrometry. Adult human bone marrow mesenchymal stem cells were cultured in extracts of 100%, 50%, 25% magnesium and eight binary magnesium alloys for 1, 3, 5, 7 days. The indirect effects of magnesium alloys on viability of adult human bone marrow mesenchymal stem cells were detected using Alamar Blue? cell viability reagent.
RESULTS AND CONCLUSION: Mg-Ca and Mg-Y alloys showed poor corrosion resistance. The Mg2+ concentration in liquid was (408.0±37.9) mg/L and (351±15.3) mg/L, respectively, while pH value was 8.87±0.19 and 8.84±0.15. Next is Mg-Zr alloy; other binary alloys were equal to pure Mg regarding corrosion resistance. The magnesium and eight binary magnesium alloys extracts at 100% concentration significantly inhibited the viability of adult human bone marrow mesenchymal stem cells. But the extracts did not inhibit the cell viability while Mg2+ concentration was ≤ 110 mg/L and pH value of 7.35-7.65.

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