中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (1): 187-190.doi: 10.3969/j.issn.1673-8225.2011.01.043

• 干细胞基础实验 basic experiments of stem cells • 上一篇    

人脐带间充质干细胞冻存复苏后细胞表面标志物分子学变化

钱燕翔1，舒  群2，蔡红霞2，郭  娟3，陈  亮1，高  峰1   

  1. 1上海市脐带血造血干细胞库，上海市  200051
    2上海市长宁区妇幼保健院，上海市  200051
    3上海市第六人民医院血液科，上海市  200233
  • 收稿日期:2010-07-06 修回日期:2010-11-01 出版日期:2011-01-01 发布日期:2011-01-01
  • 作者简介:钱燕翔★，男，1982年生，江苏省南通市人，汉族，2009年苏州大学毕业，硕士，主要从事干细胞方面的研究。

Surface marker changes in human umbilical cord-derived mesenchymal stem cells after cryopreservation and resuscitation

Qian Yan-xiang1, Shu Qun2, Cai Hong-xia2, Guo Juan3, Chen Liang1, Gao Feng1   

  1. 1Shanghai Cord Blood Bank, Shanghai  200051, China
    2Changning District Maternal and Child Health Hospital, Shanghai   200051, China
    3Department of Hematology, Shanghai Sixth People’s Hospital, Shanghai   200233, China
  • Received:2010-07-06 Revised:2010-11-01 Online:2011-01-01 Published:2011-01-01
  • About author:Qian Yan-xiang★, Master, Shanghai Cord Blood Bank, Shanghai 200051, China qianyx1982@gmail.com

PDF

473

被引次数

21

摘要：

背景：间充质干细胞是具有自我更新能力且能够多向分化的干细胞。脐带属于胚胎外组织，是胎儿分娩后的废弃物，来源广泛，无伦理学限制，因此有望作为间充质干细胞来源的第一选择。
目的：检测人脐带间充质干细胞冻存复苏前后细胞表面分子CD29、CD44、CD49e、CD73、CD90、CD34、CD45、CD271标志变化情况。
方法：通过从人的脐带中分离培养间充质干细胞，分别观察原代细胞、P4、P8代细胞和冻存复苏后P2、P4、P8代细胞形态；通过流式细胞仪检测原代细胞、P4、P8代细胞和冻存复苏后P2、P4、P8代细胞表面分子标志CD29、CD44、CD49e、CD73、CD90、CD34、CD45、CD271。
结果与结论：人脐带间充质干细胞冻存前与原代复苏后不同代数下细胞均表现出相同的表型，CD29、CD44、CD49e、CD73、CD90阳性，CD34、CD45、CD271阴性，提示人脐带间充质干细胞原代低温冻存复苏后细胞表面标志性分子无变化。

关键词: 脐带, 间充质干细胞, 冻存复苏, 表面标志, 流式细胞仪

Abstract:

BACKGROUND: Mesenchymal stem cells are the stem cells that possess the capability for self-renewal and multi-directional differentiation. Umbilical cord is the tissue outside the embryos and would be fallen off after parturition. In addition, it has wide source and no ethical restriction, so it is promising to be the first choice for mesenchymal stem cells. 
OBJECTIVE: To detect the surface markers CD29, CD44, CD49e, CD73, CD90, CD34, CD45, and CD271 of human umbilical cord-derived mesenchymal stem cells (hUCMSCs) prior to and after cryopreservation and resuscitation.
METHODS: After isolation and culture, morphology of the primary, P4 and P8 hUCMSCs was observed prior to cryopreservation and after resuscitation. Surface markers CD29, CD44, CD49e, CD73, CD90, CD34, CD45, and CD271 of primary, P4, and P8 hUCMSCs were detected through the use of flow cytometry prior to cryopreservation and after resuscitation
RESULTS AND CONCLUSION: hUCMSCs prior to cryopreservation and hUCMSCs of different passages after resuscitation present the same phenotype, i.e., positive for CD29, CD44, CD49e, CD73, and CD90, and negative for CD34, CD45, CD271. These findings suggest that primary hUCMSCs do not present changes in surface markers after cryopreservation and resuscitation.

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