中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (1): 113-115.doi: 10.3969/j.issn.1673-8225.2011. 01.025

• 干细胞学术探讨 stem cell academic discussion • 上一篇    下一篇

胚胎干细胞及在生殖医学中的应用

刘黎青1，李  娟2，周盛年3   

  1. 1山东中医药大学，山东省济南市   250355
    2山东中医药大学2008级硕士研究生，山东省济南市  250355
    3山东大学齐鲁医院，山东省济南市  250012
  • 收稿日期:2010-06-12 修回日期:2010-09-25 出版日期:2011-01-01 发布日期:2011-01-01
  • 通讯作者: 周盛年，博士生导师，教授，山东大学齐鲁医院，山东省济南市 250012 zhoushengnian126@126.com
  • 作者简介:刘黎青，女，1961年生，山东省济南市，汉族，1983年山东医学院毕业，教授，主要从事干细胞等方面的研究。 liuliqing123@tom.com
  • 基金资助:

    山东省科技攻关课题(2007GG10002017)；山东省自然基金(Y 2008C63)；山东省中医药科学技术研究项目(2009-002)。

Application of embryonic stem cells to reproductive medicine

Liu Li-qing1, Li Juan2, Zhou Sheng-nian3    

  1. 1Shandong University of Traditional Chinese Medicine, Jinan  250355, Shandong Province, China
    2Master postgraduate in 2008, Shandong University of Traditional Chinese Medicine, Jinan  250355, Shandong Province, China
    3Qilu Hospital, Shandong University, Jinan  250012, Shandong Province, China
  • Received:2010-06-12 Revised:2010-09-25 Online:2011-01-01 Published:2011-01-01
  • Contact: Zhou Sheng-nian, Doctoral supervisor, Professor, Qilu Hospital, Shandong University, Jinan 250012, Shandong Province, China zhoushengnian126@126.com
  • About author:Liu Li-qing, Professor, Shandong University of Traditional Chinese Medicine, Jinan 250355, Shandong Province, China liuliqing123@tom.com
  • Supported by:

    the Science and Technology Foundation of Shandong Province, No. 2007GG10002017; the Natural Foundation of Shandong Province, No. Y 2008C63; the Traditional Chinese Medicine Research Program of Shandong Province, No. 2009-002

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摘要：

背景：有报道胚胎干细胞体外培养可分化为原始生殖细胞并进一步生成配子，受精后发育为胚泡，并产生成活的子代。
目的：阐述近年胚胎干细胞在生殖医学中的研究进展。
方法：应用计算机检索清华同方系列数据库2000/2009有关生殖细胞、中药的相关研究文章，检索词：生殖细胞、中药，并限定文章语言种类为中文；同时应用计算机检索Pubmed数据库1998/2009相关文章，检索词：embryonic stem cells，stem cells，germ cells，oocyte，sperm，differentiation，限定文章语言种类为“English”。
结果与结论：胚胎干细胞在体外分化受到内、外源因素的影响，可以诱导分化为精子和卵母细胞，是研究哺乳动物早期胚胎发育的理想模型；目前研究体外培养原始生殖细胞和配子是可行的，但体外培养生殖细胞的特性和功能仍不确定，要应用于临床需进一步确定其安全性和有效性。

关键词: 胚胎干细胞, 生殖细胞, 中药, 模型, 不孕症

Abstract:

BACKGROUND: It has been reported that embryonic stem cells can differentiate into primordial germ cells in vitro culture and to further generate gametes then develop into blastocysts after fertilization, and produce live filial generation.
OBJECTIVE: To expound the research progress of embryonic stem cells in reproductive medicine in recent years.
METHODS: Tongfang database system was used to search for research articles published from 2000 to 2009 on germ cells and Chinese medicine related to the key words “germ cells, medicine”, and the language was limited to Chinese; the same searching PubMed database articles published from 1998 to 2009 with the key words of “embryonic stem cells, stem cells, germ cells, oocyte, sperm, differentiation”, and the language was limited to English.
RESULTS AND CONCLUSION: Embryonic stem cells in vitro differentiation affected by internal and external factors, can be induced to differentiate into sperm and oocytes, is an ideal model on the study of early embryonic development in mammals. At present, it is feasible to in vitro culture primordial germ cells and gamete, but the characteristics and function of in vitro cultured germ cells remain unclear and it is necessary to further identify its safety and efficacy in clinic.

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