中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (45): 8507-8511.doi: 10.3969/j.issn.1673-8225.2010.45.035

• 干细胞学术探讨 stem cell academic discussion • 上一篇    下一篇

间充质干细胞向肝样细胞的诱导分化

陈  军1，刘玉侠1，姜文华2   

  1. 1吉林省肿瘤防治研究所，吉林省长春市  130012；2吉林大学白求恩医学院组织胚胎教研室，吉林省长春市130021
  • 出版日期:2010-11-05 发布日期:2010-11-05
  • 通讯作者: 姜文华，博士，副教授，吉林大学基础白求恩医学院组织胚胎教研室，吉林省长春市 130021 jiangwenhua468@163.com
  • 作者简介:陈军，男， 1956年生，吉林省长春市人，汉族，教授，博士生导师，主要从事肿瘤免疫的研究。 yuhongnew@yahoo.com.cn
  • 基金资助:

    吉林省科技发展计划项目(200905200)；吉林省自然科学基金(201015197)。　　

Induced differentiation of mesenchymal stem cells into hepatocyte-like cells

Chen Jun1, Liu Yu-xia1, Jiang Wen-hua2   

  1. 1 Jilin Province Institute of Cancer Research, Changchun  130012, Jilin Province, China; 2 Department of Histology and Embryology, Norman Bethune College of Medicine, Jilin University, Changchun  130021, Jilin Province, China
  • Online:2010-11-05 Published:2010-11-05
  • Contact: Jiang Wen-hua, Doctor, Associate professor, Department of Histology and Embryology, Norman Bethune College of Medicine, Jilin University, Changchun 130021, Jilin Province, China jiangwenhua468@163.com
  • About author:Chen Jun, Professor, Doctoral supervisor, Jilin Province Institute of Cancer Research, Changchun 130012, Jilin Province, China yuhongnew@yahoo.com.cn
  • Supported by:

    the Science and Technology Development Project of Jilin Province, No. 200905200*;The Natural Science Foundation of Jilin Province , No.201015197*

PDF

317

被引次数

5

摘要：

背景：目前很多体内外实验都已表明间充质干细胞具有向肝样细胞或肝细胞分化的能力，这使间充质干细胞治疗肝脏疾病成为可能。
目的：总结和分析间充质干细胞向肝样细胞诱导分化的方法、机制以及存在的问题。
方法：应用计算机检索PubMed数据库(2000-01/2009-12)，以“mesenchymal stem cells，hepatocyte, differentiation”为检索词；应用计算机检索维普数据库(2000-01/2009-12)，以“间充质干细胞，肝细胞，诱导分化”为检索词。选择文章内容与间充质干细胞诱导分化为肝细胞相关，同一领域文献则选择近期发表或发表在权威杂志文章。
结果与结论：共收集146篇关于间充质干细胞向肝样细胞诱导分化的文章，纳入 31篇符合标准的文献。间充质干细胞可在体外及肝脏病理条件下分化为肝细胞，其作用机制主要是间充质干细胞在合适的细胞因子组合诱导下，细胞因子通过特定的细胞信号传导途径，作用于细胞核，启动或关闭相应的基因，表达特异的蛋白质，使间充质干细胞向肝细胞分化。随着研究不断得深入，这使间充质干细胞治疗肝脏疾病成为可能，但其研究有待进一步完善。

关键词: 间充质干细胞, 肝样细胞, 诱导, 分化, 细胞因子

Abstract:

BACKGROUND: At present, many experiments have shown that mesenchymal stem cells (MSCs) can differentiate into hepatocyte-like cells or hepatocytes in vitro and in vivo. This makes it possible for MSCs in treatment of liver diseases. 
OBJECTIVE: To conclude and analyze the methods, mechanism and existing problems of MSCs differentiation into hepatocyte-like cells. 
METHODS: We searched PubMed Database using computer for articles published from January 2000 to December 2009. The key words were “mesenchymal stem cells, hepatocyte, differentiation”. Vip Database was retrieved for articles published from January 2000 to December 2009. The key words were “mesenchymal stem cells, hepatocyte, induced differentiation”. Articles concerning induced differentiation of MSCs into hepatocytes, and those published recently or in authorized journals were included.
RESULTS AND CONCLUSION: A total of 146 articles addressing induced differentiation of MSCs into hepatocytes were collected, and 31 articles were included according to inclusion criteria. MSCs can be induced to differentiate into hepatocytes in vitro and under hepatic pathological condition. Its action mechanism is that cytokines via specific cell signaling pathway affected cell nucleus, started or closed corresponding gene, expressed specific protein, and induced the differentiation of MSCs into hepatocytes under suitable cytokine combination. With the development of study, this makes it possible for MSCs in treatment of liver diseases, but still requires further improvement.

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