关键词: 胎盘源间充质干细胞, 骨髓源间充质干细胞, 成脂细胞, 诱导分化, 差异

Abstract:

BACKGROUND: Human bone marrow-derived mesenchymal stem cells (BMSCs) have a great clinical value, but limited by the number and source, in contrast, human placenta-derived mesenchymal stem cells (PMSCs) have become an important cellular source in regenerative medicine and the stem cells of the most promising clinical application.
OBJECTIVE: To compare the differences in isolation and culture in vitro, amplification and differences in biological characteristics between PMSCs and BMSCs.
METHODS: Human placentas were isolated by collagenase and bone marrows were isolated by density gradient centrifugation. 10% fetal calf serum was added to the LG-DMEM culture medium and cells were digested and passaged after converging to 90%. The third generation of PMSCs and BMSCs were vaccinated in the concentration of 1 × 106 and replaced by fat cells differentiated culture medium after converging to 70%-80% and induced for 16 days.
RESULTS AND CONCLUSION: PMSCs and BMSCs had the adherent growth ability, morphology of fibroblast-like cells and spindle-shaped appearance, but the latter had the slightly smaller size. Both BMSCs and PMSCs highly expressed CD29, CD44, did not express CD34, CD106. Both could be differentiated into adipose cells. Cell morphology and growth characteristics are basically the same between BMSCs and PMSCs, both can amplify in vitro, differentiate into adipose cells and can be used as another source of adult stem cells in tissue engineering, but PMSCs have the better application prospects.