中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (44): 8179-8182.doi: 10.3969/j.issn.1673-8225.2010.44.003

• 心肺移植 heart-lung transplantation • 上一篇    下一篇

改良Heron法的同种异体心脏移植模型建立

蔡旭东，林善昌，刘与友，陈卢峰，刘玳瑄   

  1. 福建医科大学附属第二医院心胸外科，福建省泉州市  362000
  • 出版日期:2010-10-29 发布日期:2010-10-29
  • 通讯作者: 林善昌，博士，主治医师，福建医科大学附属第二医院心胸外科，福建省泉州市 362000 Lscaboy@126.com
  • 作者简介:蔡旭东，男，1966年生，福建省泉州石狮市人，汉族，1990年福建医科大学毕业，副主任医师，主要从事心胸外科基础与临床研究。 714889404@qq.com

Establishment of an allogenic heart transplant model using modified Heron’s technique

Cai Xu-dong, Lin Shan-chang, Liu Yu-you, Chen Lu-feng, Liu Dai-xuan   

  1. Department of Cardiothoracic Surgery, Second Affiliated Hospital, Fujian Medical University, Quanzhou  362000, Fujian Province, China
  • Online:2010-10-29 Published:2010-10-29
  • Contact: Lin Shan-chang, Doctor, Attending physician, Department of Cardiothoracic Surgery, Second Affiliated Hospital, Fujian Medical University, Quanzhou 362000, Fujian Province, China Lscaboy@126.com
  • About author:Cai Xu-dong, Associate chief physician, Department of Cardiothoracic Surgery, Second Affiliated Hospital, Fujian Medical University, Quanzhou 362000, Fujian Province, China

PDF

425

被引次数

5

摘要：

背景：啮齿类动物心脏移植模型分为工作模型和非工作模型，非工作模型相对于工作模型，手术时间短、成功率高，其中以Heron法最为显著。
目的：对Heron法建立大鼠心脏移植模型的手术方法进行改良，建立更合理、符合实验需要的心脏移植模型，以利于后续实验研究。
方法：对Heron法进行改良，将受体右颈外静脉套管插入供心右肺动脉，右颈总动脉套管插入供心主动脉，环扎固定，供心左肺动脉结扎，从而快速、高效地建立改良的心脏移植模型。观察大鼠移植成功率、并发症、总手术时间、受体血管套管准备时间，供心摘取时间，颈部移植时间及供心冷缺血时间。
结果与结论：单人操作连续进行正式实验25次，移植成功24次，成功率96%。全组无吻合口漏血、血液回流障碍，术后受体无死亡。每例手术总时间需50~60 min；受体血管套管准备时间为(18±3) min；供心摘取时间为(10±1) min；颈部移植时间为(6±1) min；冷缺血时间为(16±1) min。结果表明，改良Heron法建立心脏移植模型，无需显微外科操作，是一种冷缺血时间短，更合理、实用、稳定可靠和易于复制的器官移植研究动物模型。

关键词: 心脏移植, Heron法, 改良, 大鼠, 动物模型

Abstract:

BACKGROUND: Rodents animal heart transplantation model was divided into working cast and non-working casts. Compared with working cast, non-working cast is characterized by short surgical time and high success rate, especially Heron method.
OBJECTIVE: To modify Heron’s technique for the model of heart transplant in rats in order to establish a more reasonable and practicable model so that it fits for the requirement of following experiment. 
METHODS: Heron’s technique was improved. The recipient’s right external jugular vein cannula was inserted into the donor’s right pulmonary artery. Right common carotid artery cannula was inserted into the heart aorta. Cerclage was used. Donor’s heart left pulmonary artery was ligated, and then model of modified heart transplant was rapidly and effectively established. Success rate, complication, total surgical time, set up time of recipient’s vessel cannula, donor’s heart selection time, transplantation time of the neck part and cold ischemia time of donor’s heart were observed.
RESULTS AND CONCLUSION: Formal tests were consecutively performed 25 times by a single person. A total of 24 tests were successful, resulting in a success rate of 96%. There was no anastomosis leakage, recirculation disorder and recipient’s death after operation. The total time of surgical procedure was 50 to 60 minutes for each case. The time was respectively (18±3) minutes for preparing the recipient’s cervical vessel cannulas, (10±1) minutes for selecting the donor’s heart, (6±1) minutes for heart transplantation in cervical part and (16±1) minutes for cold ischemia of donor’s heart. Results suggest that the modified Heron’s technique was a more reasonable and practicable method to establish a reliable and high reproducible model of heart transplant that could be operated by surgeons without minimal microvascular technique and with short cold ischemia time.

中图分类号: