中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (41): 7683-7686.doi: 10.3969/j.issn.1673-8225.2010.41.020

• 组织构建实验造模 experimental modeling in tissue construction • 上一篇    下一篇

应用单宁酸-氯化铁法筛选长爪沙鼠脑底动脉Willis环变异

吴晓光，郭园园，颜  勇，赵淑敏，龚秀云   

  1. 承德医学院基础医学研究所，河北省承德市 067000
  • 出版日期:2010-10-08 发布日期:2010-10-08
  • 通讯作者: 赵淑敏，教授，硕士生导师，承德医学院电镜室，河北省承德市 067000 zhaoshumin-2008@163.com
  • 作者简介:吴晓光★，男，1975年生，河北省承德市人，满族，2008年华北煤炭医学院毕业，硕士，实验师，主要从事脑血管病相关研究。 ewxg@qq.com
  • 基金资助:

    承德市科技局(200621006，200721073)。课题名称：补阳还五汤对脑缺血再灌注损伤后微循环变化的影响；单宁酸-氯化铁法筛选脑底动脉Willis环结构变异沙土鼠的探索。

Mutation of cerebral artery Willis circle of Mongolian gerbils screened using tannic acid-ferric chloride staining

Wu Xiao-guang, Guo Yuan-yuan, Yan Yong, Zhao Shu-min, Gong Xiu-yun   

  1. Department of Preclinical Medicine, Chengde Medical College, Chengde  067000, Hebei Province, China
  • Online:2010-10-08 Published:2010-10-08
  • Contact: Zhao Shu-min, Professor, Master’s supervisor, Department of Preclinical Medicine, Chengde Medical College, Chengde 067000, Hebei Province, China zhaoshumin-2008@163.com
  • About author:Wu Xiao-guang★, Master, Experimentist, Department of Preclinical Medicine, Chengde Medical College, Chengde 067000, Hebei Province, China ewxg@qq.com
  • Supported by:

     the Foundation of Science and Technology Bureau, No. 200621006*，200721073*

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摘要：

背景：以往曾采用阳性体征来判断长爪沙鼠脑缺血的有无，但存在人为影响因素较大、指标不严谨等缺点。单宁酸-氯化铁法媒染微血管效果可靠，方法简捷易行，重复性好，有可能从血管角度筛选以克服上述缺点。
目的：探索一种简便、可靠的筛选长爪沙鼠脑底Willis环变异的方法。
方法：根据Kirino法制作长爪沙鼠前脑缺血模型，激光多普勒血流仪监测脑组织血流量，单宁酸-氯化铁法显示脑组织微血管。
结果与结论：夹闭双侧颈总动脉后，多普勒监测到前脑仍存在血流量的长爪沙鼠，经单宁酸-氯化铁染色证实均存在Willis环变异。研究表明应用单宁酸-氯化铁法显示微血管，可简便、可靠地筛选出脑底Willis环变异的长爪沙鼠。

关键词: 单宁酸-氯化铁法, 长爪沙鼠, Willis环, 变异, 脑缺血模型

Abstract:

BACKGROUND: Previously, whether the brain ischemia is exist or not judged by positive syndrome, which would be influenced artificially, and the target is also not strictly. The tannic acid-ferric chloride method is characterized by reliable, simple and reproducible. Accordingly, this method can make it possible for the scientific researcher to overcome the above deficiencies by screening cerebral artery circle of Willis of Mongolian gerbils.
OBJECTIVE: To explore a simple and reliable method for screening the Willis circle variation of arteries in Mongolian gerbils.
METHODS: The forebrain ischemia models of Mongolian gerbils were established by using Kirino’s method. Laser spectroscopy was used to observe the blood flow in the cerebral artery, and tannic acid-ferric chloride method was used to display cerebral microvasculars.
RESULTS AND CONCLUSION: After common carotid arteries were legend, those Mongolian gerbils, which laser spectroscopy was still able to detect the blood flow in forebrain, have circle of Willis arteries, as confirmed by tannic acid-ferric chloride method. The results demonstrated that it is quickly and reliably to screen Willis circle variation in Mongolian gerbils using tannic acid-ferric chloride method to display brain microvasculars.

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