中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (41): 7601-7604.doi: 10.3969/j.issn.1673-8225.2010.41.001

• 软骨组织构建 cartilage tissue construction •    下一篇

降钙素对关节软骨细胞中β连环蛋白和分泌型frizzled相关蛋白1的影响

张尹娜1，王文雅1，张  柳2，胡宏宇2，李  彬2，王哲彦1   

  1. 1华北煤炭医学院病理教研室，河北省唐山市 063000；2华北煤炭医学院附属医院骨外科，河北省唐山市  063000
  • 出版日期:2010-10-08 发布日期:2010-10-08
  • 通讯作者: 王文雅，教授，华北煤炭医学院，河北省唐山市 063000 wangwenya-3@126.com
  • 作者简介:张尹娜★，女，1981年生，河北省唐山市人，汉族，2006年华北煤炭医学院毕业，硕士，主要从事骨性关节炎研究。 chengt2080@yahoo.com.cn
  • 基金资助:

    河北省科技领军人才创新基金项目(06547008D-8)。

Effect of calcitonin on beta-catenin and secreted frizzled-related protein 1 in articular cartilages

Zhang Yin-na1, Wang Wen-ya1, Zhang Liu2, Hu Hong-yu2, Li Bin2, Wang Zhe-yan1   

  1. 1 Department of Pathology, North China Coal Medical College, Tangshan  063000, Hebei Province, China; 2 Department of Orthopedic Surgery, Affiliated Hospital of North China Coal Medical College, Tangshan  063000, Hebei Province, China
  • Online:2010-10-08 Published:2010-10-08
  • Contact: Wang Wen-ya, Professor, Department of Pathology, North China Coal Medical College, Tangshan 063000, Hebei Province, China Wangwenya-3@126.com
  • About author:Zhang Yin-na★, Master, Department of Pathology, North China Coal Medical College, Tangshan 063000, Hebei Province, China chengt2080@yahoo.com.cn
  • Supported by:

    the Science and Technology Innovation Leading Talent Fund Project of Hebei Province, No. 06547008D-8*

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摘要：

背景：研究表明降钙素具有抗骨吸收作用，对软骨有保护作用，但其具体机制尚不明确。
目的：探讨降钙素是否通过Wnt/β信号通路对关节软骨细胞起保护作用。
方法：分离培养雄性日本大耳白兔关节软骨细胞，随机分为对照组，白细胞介素1β组和降钙素组，后两组细胞于传代后的第2天加入白细胞介素1β (1×10-5 g/L)模拟骨关节炎发生，降钙素组第4天换成含有降钙素的完全培养基。第6天采用免疫细胞化学法检测β连环蛋白的表达，以及实时荧光定量PCR法检测β连环蛋白与分泌型frizzled相关蛋白1 mRNA的表达。
结果与结论：降钙素组β连环蛋白和mRNA的表达显著低于白细胞介素1β组 (P < 0.05)；白细胞介素1β组β连环蛋白和mRNA的表达显著高于对照组 (P < 0.05)。降钙素组分泌型frizzled相关蛋白1的mRNA表达显著高于白细胞介素1β组(P < 0.05)，白细胞介素1β组分泌型frizzled相关蛋白1 mRNA表达显著低于对照组(P < 0.05)。这些结果说明降钙素能抑制关节软骨细胞的进一步损伤，可能是通过Wnt/β连环蛋白信号通路对关节软骨细胞起保护作用。

关键词: 降钙素, 骨性关节炎, 白细胞介素1&beta, &beta, 连环蛋白, 分泌型frizzled相关蛋白1

Abstract:

BACKGROUND: Researches have reported the protection function of calcitonin for chondrocytes via preventing bone resorption, but the mechanism remains unclear.
OBJECTIVE: To assess whether calcitonin exerts a protective action through the Wnt signal pathway in chondrocytes.
METHODS: The chondrocytes were separated from Japanese male rabbits and randomly divided into the control, interleukin 1 β and calcitonin groups. In the later two groups, interleukin 1 β (1×10-5 g/L) was added at 2 days after passage to simulate osteoarthritis, and then DMEM containing calcitonin was used to culture the cells in the calcitonin group at 4 days after passage. The expression of β-catenin protein was detected by immunocytochemistry, and the mRNA of β-catenin and secreted frizzled-related protein 1 (sFRP-1) were determined by real-time PCR. 
RESULTS AND CONCLUSION: The expression of β-catenin and its mRNA in the calcitonin group was obviously lower than that of the interleukin 1 β group (P < 0.05). Compared with the control group, the expression of β-catenin and its mRNA in the interleukin 1 β group appeared to be greater (P < 0.05). The expression of sFRP-1 mRNA was notably higher in the calcitonin group than that of the interleukin 1 β group (P < 0.05), which was lower than the control group (P < 0.05). The results demonstrated that calcitonin can inhibit the further damage of articular cartilage, which may play its protective role via Wnt/β-catenin signal pathway.

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