中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (40): 7470-7473.doi: 10.3969/j.issn.1673-8225.2010.40.013

• 干细胞移植 stem cell transplantation • 上一篇    下一篇

人脐带间充质干细胞不同输注途径对急性肾小管坏死的影响

李  芳1，胡  祥1，2，赵红梅1，贾丹兵1，党智杰1   

  1. 1解放军第211医院肾内科，黑龙江省哈尔滨市  150086；2深圳市北科细胞工程研究所，广东省深圳市  518000
  • 出版日期:2010-10-01 发布日期:2010-10-01
  • 作者简介:李芳☆，女， 1963年生，上海市人，回族，2000年哈尔滨医科大学毕业，博士，主任医师，主要从事肾脏病学研究。 weizhaohongmei@163.com

Influence of different infusion methods of human umbilical cord mesenchymal stem cells on acute tubular necrosis

Li Fang1, Hu Xiang 1, 2, Zhao Hong-mei1, Jia Dan-bing1, Dang Zhi-jie1   

  1. 1 Department of Nephrology, the 211 Hospital of Chinese PLA, Harbin  150086, Heilongjiang Province, China; 2 Shenzhen Beike Cells Engineering Research Institute, Shenzhen  518000, Guangdong Province, China
  • Online:2010-10-01 Published:2010-10-01
  • About author:Li Fang☆, Doctor, Chief physician, Department of Nephrology, the 211 Hospital of Chinese PLA, Harbin 150086, Heilongjiang Province, China weizhaohongmei@163.com

PDF

424

被引次数

17

摘要：

背景：研究已证实外源性间充质干细胞能够迁移、定居于缺血再灌注损伤后的肾组织中分化为肾小管上皮细胞，恢复肾脏组织结构和功能。目前对以动脉注射或静脉注射途径移植干细胞哪种更好仍存在争议。
目的：观察人脐带间充质干细胞经不同途径移植对家犬急性肾小管坏死的影响。
方法：建立健康家犬急性肾小管坏死模型，以随机编号法分为2组，分别以外周静脉注射及右股动脉介入经肾动脉直接注射DAPI标记的人脐带间充质干细胞。移植后24 h，光镜下观察肾组织形态改变，荧光显微镜下观察人脐带间充质干细胞植入实验动物体内后的存活情况。
结果与结论：普通光镜下观察可见两组动物肾小管上皮细胞均出现广泛肿胀、变性、坏死等病理变化。经外周静脉注射及经肾动脉直接注射的人脐带间充质干细胞均在病损肾脏中成活，且两组荧光强度差异无显著性意义(P > 0.05)。说明两种途径移植人脐带间充质干细胞均可在肾脏内定殖，修复肾脏组织结构的效果相当，相对于动脉注射途径，经静脉注射移植途径创伤较小。

关键词: 移植途径, 人脐带间充质干细胞, 急性肾小管坏死, 干细胞, 干细胞移植

Abstract:

BACKGROUND: Studies have confirmed that exogenous mesenchymal stem cells can migrate and establish in the nephridial tissue following ischemia/reperfusion injury, differentiate into renal tubular epithelial cells, and recover structure and function of the kidney. At present, it is controversial which is better, arterial injection or intravenous injection, for transplanting stem cells.
OBJECTIVE: To observe the effects of different transplantation methods of human umbilical cord mesenchymal stem cells on dogs with acute tubular necrosis (ATN). 
METHODS: Healthy dog models of ATN were established and randomly divided into two groups. Human umbilical cord derived mesenchymal stem cells were labeled with DAPI. Then the cells were injected through peripheral veins and right femoral artery into model dogs’ body. 24 hours later, histological changes were observed by light microscope, and the survival of human umbilical cord mesenchymal stem cells was evaluated by fluorescent microscope.
RESULTS AND CONCLUSION: Under common optical microscope, pathological changes such as swelling, degeneration and necrosis appeared in renal tubular epithelial cells in both groups. The injected human umbilical cord mesenchymal stem cells could survive in the affected kidney. There was no significant difference on fluorescence intensity between two groups (P > 0.05). These indicated that transplanted human umbilical cord mesenchymal stem cells by two pathways can survive in the kidneys. The outcomes were similar in repairing the kidney. Compared with arterial injection, the transplantation of intravenous injection obtained little trauma.

中图分类号: