中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (38): 7065-7068.doi: 10.3969/j.issn.1673-8225.2010.38.009

• 膜生物材料 membrane biomaterials • 上一篇    下一篇

丝素蛋白作为真皮替代物移植后的组织学变化

李  丽1，张印峰1，李  克1，李永林2　   

  1. 1苏州大学附属第一医院烧伤整形科，江苏省苏州市   215006；2苏州美莱美容医院，江苏省苏州市   215006
  • 出版日期:2010-09-17 发布日期:2010-09-17
  • 通讯作者: 李永林，博士，副教授，硕士生导师，苏州美莱美容医院，江苏省苏州市 215006 yonglinl@hotmail.com
  • 作者简介:李丽★，女，1985年生，江苏省苏州市人，汉族，苏州大学在读硕士，主要从事创面损伤与修复研究。82185904@qq.com
  • 基金资助:

    国家自然科学基金(30970714)。

Histological changes of the grafted dermal substitutes made of silk fibroin

Li Li1, Zhang Yin-feng1, Li Ke1, Li Yong-lin2   

  1. 1 Department of Burns and Plastic Surgery, First Affiliated Hospital of Soochow University, Suzhou   215006, Jiangsu Province, China; 2 My Like Cosmetology Hospital of Suzhou, Suzhou   215006, Jiangsu Province, China　
  • Online:2010-09-17 Published:2010-09-17
  • Contact: Li Yong-lin, Doctor, Associate professor, Master’s supervisor, My Like Cosmetology Hospital of Suzhou, Suzhou 215006, Jiangsu Province, China yonglinl@163.com
  • About author:Li Li★,Studying for master’s degree, Department of Burns and Plastic Surgery, First Affiliated Hospital of Soochow University, Suzhou 215006, Jiangsu Province, China 82185904@qq.com
  • Supported by:

     the National Natural Science Foundation of China, No. 30970714*

PDF

503

被引次数

10

摘要：

背景：目前国外研究者已研发出多种真皮替代物，取得了一定临床效果，但价格非常昂贵，因此研制和开发经济实用的高质量真皮替代物具有重要意义。
目的：了解以柞蚕丝素蛋白材料为支架构建组织工程真皮替代物移植后的组织学变化，并与聚乙烯醇海绵相比较。
方法：取清洁级雄性SD大鼠，背部制作一大小为20 mm×20 mm 的全层皮肤缺损创面，切下皮肤反取成刃厚皮待用，然后随机分为2组，海绵组：创面埋植聚乙烯醇海绵并覆盖自体表皮。丝素组：创面埋植交联柞蚕丝素多孔材料并覆盖自体表皮。分别于术后5，10，15，25 d取材，观察移植物及其周围组织大体标本和组织形态学变化，免疫组织化学染色增殖细胞核抗原和转化生长因子β1的相对定量分析。
结果与结论：移植术后丝素组复合皮片生长良好、全部存活；海绵组移植皮片大部分变黑、结痂，并有脱落。免疫组织化学结果显示丝素组在术后5~25 d 转化生长因子β1和增殖细胞核抗原的表达均显著大于海绵组。结果提示，以丝素蛋白为支架构建的组织工程真皮替代物具有良好的生物相容性,血管化过程快，并能促进皮肤创面的愈合。

关键词: 丝素蛋白, 真皮替代物, 生物相容性, 增殖细胞核抗原, 转化生长因子&beta, 1

Abstract:

BACKGROUND: A variety of dermal substitutes with effective clinical results have now been developed by foreign researchers. But the price is very expensive, so it’s important to develop economical dermal substitutes with high quality.
OBJECTIVE: To observe the histological changes in the dermal substitutes made of China oak silk fibroin after grafting, and to compare with polyvinyl alcohol (PVA) sponge.
METHODS: A full-thickness skin defect at the size of 20 mm × 20 mm was made in the back of clean male SD rats, the skin was cur off into split-thick skin, which were randomized into two groups. Composite transplant of silk fibroin, as a new dermal surrogate, and autologous epidermis was grafted to repair wounds in the experimental group. Composite transplant of polyvinylalcohol sponge, as a new dermal surrogate, and autologous epidermis was grafted to repair wounds in the control group. The tissue biopsies were harvested at 5, 10, 15, 25 post grafting days. The gross appearance and histological changes of the grafts and surrounding tissue were observed. Relative quantitative analysis of transforming growth factor-β1 and proliferating cell nuclear antigen were detected by immunohistochemical method.
RESULTS AND CONCLUSION: The composite skin grafts grew and survived well in the silk fibroin group, while most of the transplants in the sponge group were necrotic and scabbed, even dropped. Immunohistochemical assay showed that the levels of transforming growth factor-β1 and proliferating cell nuclear antigen expression were dramatically higher in silk fibroin group than those in the sponge group during 5 to 25 post grafting days. The revascularization of tissue engineered dermal substitutes made of silk fibroin could begin shortly after grafting, the biocompatibility of silk fibroin is fantastic and it can facilitate the healing of skin wounds.

中图分类号: