中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (23): 4262-4266.doi: 10.3969/j.issn.1673-8225.2010.23.019

• 干细胞移植 stem cell transplantation • 上一篇    下一篇

同种异体大鼠骨髓间充质干细胞移植在肠缺血-再灌注损伤肠道中的定植及其分化效应

高广周1，李大伟2，李 欣3，孙 涛3   

  1. 1解放军第二军医大学海军临床医学院，北京市 100037；解放军第二军医大学海军临床医学院，2ICU，3消化内科，北京市 100037
  • 出版日期:2010-06-04 发布日期:2010-06-04
  • 通讯作者: 孙 涛，教授，主任医师，硕士生导师，解放军第二军医大学海军临床医学院消化内科，北京市 100037
  • 作者简介:高广周，男，1983 年生，河北省保定市人，汉族，解放军第二军医大学在读硕士，医师，主要从事胃肠疾病干细胞治疗的研究。 gaoguangzhou@yahoo.com.cn
  • 基金资助:

    全军“十一五”面上项目(06MA017)

Colonization and differentiation of allogeneic rat bone marrow mesenchymal stem cells transplanted into the intestine with ischemia/reperfusion injury

Gao Guang-zhou1, Li Da-wei2, Li Xin3, Sun Tao3   

  1. 1Navy Clinical Medical College, Second Military Medical University of Chinese PLA, Beijing  100037, China; 2ICU, 3Department of Gastroenterology, Second Military Medical University of Chinese PLA, Beijing  100037, China
  • Online:2010-06-04 Published:2010-06-04
  • Contact: Sun Tao, Professor, Chief physician, Master’s supervisor, Department of Gastroenterology, Second Military Medical University of Chinese PLA, Beijing 100037, China
  • About author:Gao Guang-zhou, Studying for master’s degree, Physician, Navy Clinical Medical College, Second Military Medical University of Chinese PLA, Beijing 100037, China gaoguangzhou@ yahoo.com.cn
  • Supported by:

    the Military General Program during the 11th Five-Year Plan Period, No. 06MA017*

PDF

421

被引次数

11

摘要：

背景：小肠对缺血缺氧极为敏感，其发生缺血-再灌注时可出现严重的组织损伤,骨髓间充质干细胞具有多向分化潜能，可通过多种途径参与受损组织的修复。

目的：观察同种异体大鼠骨髓间充质干细胞移植后在肠缺血-再灌注损伤中肠道的定植情况和治疗效果。

方法：Wistar雌性大鼠分为3组，假手术组剖开腹腔后即予以缝合；其余大鼠建立肠缺血-再灌注模型，对照组肠道缺血-再灌注后仅输入生理盐水；治疗组鼠尾静脉注射Wistar雄性大鼠骨髓间充质干细胞。治疗后分别于12，24 h，3，7，14，28 d分批取空肠组织，制作冰冻切片在荧光显微镜观察供体细胞在受体肠道的分布，空肠组织匀浆后行RT-PCR检测雄性大鼠的性别决定基因，并检测肠组中丙二醛和超氧化物歧化酶含量。

结果与结论：冰冻切片在荧光显微镜下观察，未见供体细胞在受体肠绒毛表面定植。雌性大鼠肠组织中性别决定基因的RT-PCR检测结果为：3 d阳性率为50%，7 d阳性率66.6%，14 d阳性率33.3%，28 d阳性率为16.6%。骨髓间充质干细胞治疗组第12，24小时，3，7天的空肠组织中的丙二醛水平低于对照组、超氧化物歧化酶含量高于对照组。结果提示，同种异体大鼠骨髓间充质干细胞可在受体肠缺血-再灌注损伤的肠道内定植，并可加速肠道损伤的恢复，其发挥疗效并非直接分化，而是主要是通旁分泌的形式促进机体内源性修复。

关键词: 肠缺血-再灌注, 骨髓间充质干细胞, 大鼠, 疗效, 肠道

Abstract:

BACKGROUND: The small intestine is extremely sensitive to the hypoxia and ischemia, and it would be got severe damage after ischemia/reperfusion injury. Bone marrow mesenchymal stem cells (BMSCs) are multipotent cells which might participate in the repair of damaged tissue through a variety of ways.

OBJECTIVE: To investigate colonization and therapeutic effects of allogeneic rat BMSCs transplantation in the intestine of ischemia/reperfusion injury model.

METHODS: Wistar female rats were assigned to three groups. The abdominal cavity was opened and then sutured in the sham operation group. Remaining rats were used to establish ischemia/reperfusion models. Rats in the control group were only infused with saline following intestine with ischemia/reperfusion injury. Rats in the treatment group were treated with BMSCs from Wistar male rats via caudal vein. Following treatment, jejunal tissue was harvested at 12, 24 hours, 3, 7, 14 and 28 days separately to make frozen sections. The distribution of donor cells in the receptor intestine was observed under a fluorescence microscope. Reverse transcription-polymerase chain reaction was employed to determine the sex-determining gene (SRY) in male rats. The levels of malondialdehyde (MDA) and superoxide dismutase (SOD) in the jejunal tissues were detected.

RESULTS AND CONCLUSION: No donor cell transfused in intestinal villi was detected under the fluorescence microscope. The expression ratio of the SRY gene was 50%, 66.6%, 33.3%, 16.6% at 3, 7, 14, and 28 days respectively. The levels of MDA were lower, but SOD levels were higher in jejunum tissues in the treatment group than the control group on 12, 24 hours, 3 and 7 days. Results have found that BMSCs from allogeneic rats could colonize in the injured intestine of the receptor rat with ischemia/reperfusion injury. The BMSCs transplantation can promote the recovery of intestinal injury. The beneficial effects of BMSCs were primarily mediated via paracrine actions but not by their direct differentiation into target cells.

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