中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (23): 4231-4234.doi: 10.3969/j.issn.1673-8225.2010.23.012

• 干细胞移植 stem cell transplantation • 上一篇    下一篇

经静脉途径移植骨髓间充质干细胞在慢性心肌梗死大鼠体内的分布

杨志凯1，2，王 炜1，2，高秉仁1，金培峰2，胡盛寿2，张 浩2   

  1. 1兰州大学第二医院心胸外科，甘肃省兰州市  730030；
    2中国医学科学院阜外心血管病医院，卫生部心血管疾病再生医学重点实验室，北京市  100037
  • 出版日期:2010-06-04 发布日期:2010-06-04
  • 通讯作者: 高秉仁，教授，主任医师，博士生导师，兰州大学第二医院心胸外科，甘肃省兰州市 730030 gaobr1956@ 163.com
  • 作者简介:杨志凯，男，1984年生，河南省新乡市人，汉族，2010年兰州大学毕业，硕士，主要从事干细胞在心脏外科中的应用。 yangzhk07@ yahoo.cn

Distribution of bone marrow mesenchymal stem cells intravenously transplantated in chronic myocardial infarction rats

Yang Zhi-kai1,2, Wang Wei1,2, Gao Bing-ren1, Jin Pei-feng2, Hu Sheng-shou2, Zhang Hao2   

  1. 1Department of Cardiac and Thoracic Surgery, Second Hospital, Lanzhou University, Lanzhou  730030, Gansu Province, China;
    2Cardiovascular Institute and Fuwai Hospital, Chinese Academy of Medical Sciences, Key Laboratory of Cardiovascular Regenerative Medicine, Ministry of Health, Beijing  100037, China
  • Online:2010-06-04 Published:2010-06-04
  • Contact: Gao Bing-ren, Professor, Chief physician, Doctoral supervisor, Department of Cardiac and Thoracic Surgery, Second Hospital, Lanzhou University, Lanzhou 730030, Gansu Province, China gaobr1956@163.com
  • About author:Yang Zhi-kai, Master, Department of Cardiac and Thoracic Surgery, Second Hospital, Lanzhou University, Lanzhou 730030, Gansu Province, China; Cardiovascular Institute and Fuwai Hospital, Chinese Academy of Medical Sciences, Key Laboratory of Cardiovascular Regenerative Medicine, Ministry of Health, Beijing 100037, China yangzhk07@yahoo. cn

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摘要：

背景：目前的研究中，大多针对急性心肌梗死进行细胞移植研究，而对于慢性心肌梗死后经静脉移植干细胞的研究尚少见，尤其是在移植后多个时间点动态监测移植的干细胞在体内分布及存活情况尚无报道。

目的：观察经静脉途径移植骨髓间充质干细胞后在慢性心肌梗死模型大鼠体内的分布情况。

方法：从雄性SD大鼠获取培养骨髓间充质干细胞，将18只雌性SD大鼠制备成心肌梗死3周后随机均分为2组，实验组：用胰岛素针抽取加入PBS的含有5×106骨髓间充质干细胞的混悬液300 μL，并将其缓慢注入股静脉；对照组：注入同等体积的PBS。另选9只雌性SD大鼠仅开胸，不结扎冠状动脉，作为假手术组，并将含有5×106骨髓间充质干细胞的混悬液300 μL通过股静脉注入体内。骨髓间充质干细胞移植24 h，2周及1个月后，各组大鼠体内的细胞分布情况应用实时荧光定量PCR技术进行检测。

结果与结论：实验组与假手术组大鼠在细胞移植后1 d，肺组织、肝脏、脾脏中细胞分布差异无显著性意义(P > 0.05)，而实验组心脏中细胞分布明显多于假手术组(P < 0.01)；移植后2周，两组肺组织中细胞数目均急剧减少(P < 0.05)，肝脏、脾脏、心脏中细胞分布差异无显著性意义(P > 0.05)；移植4周后两组相比，肺组织、肝脏、脾脏中细胞分布差异亦无显著性意义(P > 0.05)，而假手术组心脏中未检测到细胞。移植4周后，各组织中细胞分布均明显减少。在对照组中，各时间点均未检测到SRY基因。提示慢性心肌梗死经静脉移植骨髓间充质干细胞后，早期大量细胞滞留于肺组织，且细胞数目随时间延长锐减。此外，心脏，脾脏，肝脏中仍可有少量细胞分布。

关键词: 骨髓间充质干细胞, 慢性心肌梗死, 细胞移植, 细胞分布, 大鼠

Abstract:

BACKGROUND: Present studies mainly focused on acute myocardial infarction in research of cell transplantation. There are a few studies addressing stem cells intravenously transplanted following chronic myocardial infarction. In particular, there are no reports concerning distribution and survival of transplanted stem cells at various time points following transplantation by dynamic monitoring.

OBJECTIVE: To observe distribution of bone marrow mesenchymal stem cells (BMSCs) intravenously transplanted in rats with chronic myocardial infarction.

METHODS: BMSCs from male Sprague Dawley rats were cultured for transplantation. Three weeks after induction of myocardial infarction, 18 female Sprague Dawley rats were randomized into 2 groups. Suspension of phosphate buffered saline (PBS) and BMSCs (5×106, 300 μL) were injected into the femoral vein of experimental group (n = 9). Meanwhile, an equal volume of PBS was injected into the femoral vein of control group (n = 9). Another 9 female Sprague Dawley rats that underwent thoracotomy were only involved as sham operation group, without ligation of coronary artery. Suspension containing BMSCs (5×106, 300 μL) was injected by the same way in the sham operation group. 300 μL suspension supplemented with 5×106 BMSCs was infused in the femoral vein. At 24 hours, 2 weeks and 1 month following BMSCs transplantation, BMSCs distribution was evaluated through real-time fluorescent quantitative polymerase chain reaction.

RESULTS AND CONCLUSION: At 1 day after BMSCs injection, no significant difference in cell distribution was detected in the lung, liver and spleen (P > 0.05). However, cell number in the heart was significantly greater in the experimental group than in the sham operation group (P < 0.01). At 2 weeks following transplantation, cell number was significantly decreased in the lung in both groups (P < 0.05), and there were no significant differences in cell distribution among liver, spleen and heart (P > 0.05). At 4 weeks following transplantation, no significant difference was detected in the lung, liver and spleen in both groups (P > 0.05). No cells were detected in the heart in the sham operation group. At 4 weeks following transplantation, cell distribution was significantly decreased in various tissues. SRY gene was not detected at various time points in the control group. Above-mentioned results have suggested that a large number of BMSCs intravenously transplanted were detected in the lung in rats with chronic myocardial infarction. Moreover, cell number was decreased over time. In addition, a few cells were detected in the heart, spleen and liver.

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