中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (6): 1134-1137.doi: 10.3969/j.issn.1673-8225.2010.06.039

• 干细胞学术探讨 • 上一篇    下一篇

急性白血病骨髓单个核细胞糖基化磷脂酰肌醇特异性磷脂酶D的表达

肖广芬1，陈方平2，王光平2，付 斌2，谢俊明2，程英妮2，李 群2，蹇在伏2   

  1. 1中南大学湘雅三医院血液科，湖南省长沙市  410013 
    2中南大学湘雅医院血液科，湖南省长沙市  410008
  • 出版日期:2010-02-05 发布日期:2010-02-05
  • 通讯作者: 陈方平，硕士，教授，博士生导师，中南大学湘雅医院血液科，湖南省长沙市 410008
  • 作者简介:肖广芬，女，1968年生，湖南省汉寿县人，汉族，2005年中南大学毕业，博士，硕士生导师，副教授，主要从事白血病方面的研究。

Glycosylphosphatidilinoditol-specific phospholipase    D expression in bone marrow mononuclear cells derived from acute leukemia patients

Xiao Guang-fen1, Chen Fang-ping2, Wang Guang-ping2, Fu Bin2, Xie Jun-ming2, Cheng Ying-ni2, Li Qun2, Jian Zai-fu2   

  1. 1Department of Hematology, Third Xiangya Hospital, Central South University, Changsha   410013, Hunan Province, China;
    2Department of Hematology, Xiangya Hospital, Central South University, Changsha   410008, Hunan Province, China
  • Online:2010-02-05 Published:2010-02-05
  • Contact: Chen Fang-ping, Master, Professor, Doctoral supervisor, Department of Hematology, Xiangya Hospital, Central South University, Changsha 410008, Hunan Province, China henfp@public.cs.hn.cn
  • About author:Xiao Guang-fen, Doctor, Associate professor, Master’s supervisor, Department of Hematology, Third Xiangya Hospital, Central South University, Changsha 410013, Hunan Province, China xgftjx2003@yahoo.com.cn

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摘要：

背景：据作者查新检索，国内外有关急性白血病骨髓单个核细胞糖基化磷脂酰肌醇特异性磷脂酶D活性及mRNA表达与白血病类型、患者肝脾淋巴结肿大关系的报道罕见。

目的：探讨糖基化磷脂酰肌醇特异性磷脂酶D的表达与急性白血病类型、急性髓系白血病患者肝脾淋巴结肿大的关系。

方法：急性髓系白血病组骨髓标本43例，急性淋巴细胞白血病组骨髓标本28例，以21例健康志愿者作为正常对照组。密度梯度离心法获得骨髓单个核细胞，用GPI锚定的胎盘碱性磷酸酶作为底物，Triton-X114分相法检测糖基化磷脂酰肌醇特异性磷脂酶D的活性，半定量RT-PCR法检测糖基化磷脂酰肌醇特异性磷脂酶D mRNA的表达，并分析糖基化磷脂酰肌醇特异性磷脂酶D活性和mRNA表达水平与急性白血病类型、急性髓系白血病患者肝脾淋巴结肿大的关系。

结果与结论：与正常对照组比较，急性髓系白血病组骨髓单个核细胞糖基化磷脂酰肌醇特异性磷脂酶D活性和mRNA表达均明显升高(P < 0.01)；急性淋巴细胞白血病组骨髓单个核细胞糖基化磷脂酰肌醇特异性磷脂酶D活性和mRNA表达均明显降低(P < 0.01)。在43例急性髓系白血病患者中，13例患者检查发现有肝脾淋巴结肿大，30例患者检查无肝脾淋巴结肿大，无肝脾淋巴结肿大标本骨髓单个核细胞糖基化磷脂酰肌醇特异性磷脂酶D活性和mRNA表达均明显高于有肝脾淋巴结肿大标本(P < 0.05)。结果证实急性白血病骨髓单个核细胞糖基化磷脂酰肌醇特异性磷脂酶D的活性与mRNA变化一致，其表达水平与急性白血病类型及急性髓系白血病患者有无肝脾和/或淋巴结肿大有关。

关键词: 白血病, 糖基化磷脂酰肌醇特异性磷脂酶D, 髓外浸润, 骨髓单个核细胞, 干细胞

Abstract:

BACKGROUND: The correlation of gycosylphosphatidilinoditol-specific phospholipase D (GPI-PLD) activity, mRNA expression to leukemia type, hepatosplenomegaly and/or lymphadenopathy has been rarely reported.
OBJECTIVE: To explore the correlation of GPI-PLD expression to leukemia type and hepatosplenomegaly and/or lymphadenopathy of acute myeloid leukemia (AML) patients. 
METHODS: Fresh bone marrow specimens were obtained from 43 newly diagnosed AML patients, 28 acute lymphocytic leukemia (ALL) patients, and 21 normal persons. Bone marrow mononuclear cells were harvested by density gradient centrifugation. GPI-anchored human placent alkaline phosphatase was used as substrate. GPI-PLD activity was determined bytriton-X114 phase partitioning procedure. GPI-PLD mRNA expression was detected by semi-quantitative RT-PCR. The relationship of GPI-PLD activity, mRNA expression and leukemia type, hepatosplenomegaly and/or lymphadenopathy was analyzed.
RESULTS AND CONCLUSION: Compared with control group, GPI-PLD activity and mRNA expression in bone marrow mononuclear cells were significantly higher in AML group (P < 0.01), while they were significantly lower in the ALL group (P < 0.01). Of 43 patients with AML patients, 13 patients had hepatosplenomegaly and/or lymphadenopathy. The GPI-PLD activity (%) and mRNA expression were significantly higher in AML patients without hepatosplenomegaly and lymphadenopathy than those patients with hepatosplenomegaly and/or lymphadenopathy (P < 0.05). These results demonstrated that GPI-PLD activity alteration is consistent with GPI-PLD mRNA expression in AML patients, and the expression levels correlate to leukemia type and hepatosplenomegaly and/or lymphadenopathy of AML patients.

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