中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (2): 209-213.doi: 10.3969/j.issn.1673-8225.2010.02.005

• 骨组织构建 bone tissue construction • 上一篇    下一篇

不同浓度凝血酶对富血小板血浆修复颅骨缺损的影响　

林敏魁1，2 ，陈小玲1，赵  欣1，2，闫福华1，2   

  1. 1福建医科大学附属口腔医院牙周科，福建省福州市350002；2福建医科大学口腔组织工程研究室，福建省福州市 350002
  • 出版日期:2010-01-08 发布日期:2010-01-08
  • 通讯作者: 闫福华，教授，博士生导师，福建医科大学附属口腔医院，福建医科大学口腔组织工程研究室，福建省福州市 350002 fhyan2005@126.com
  • 作者简介:林敏魁★，男，1973年生，福建省长乐市人，汉族，2004年福建医科大学口腔医学院毕业，硕士，副主任医师，主要从事牙周再生的基础和临床研究。 linmk105@yahoo.com.cn
  • 基金资助:

    课题受福建省教育厅(资助省属高校)项目(2008F5023)、福建省教育厅项目(JA04207)和福建省卫生厅青年基金项目(2008-1-40)等不同程度的资助。

Effect of platelet-rich plasma, activated by different concentrations of thrombin, on the repair of cranial defects

Lin Min-kui 1,2 , Chen Xiao-ling1, Zhao Xin 1,2 ,  Yan Fu-hua 1,2   

  1. 1 Department of Periodontology, Affiliated Stomatological Hospital, Fujian Medical University, Fuzhou   350002, Fujian Province, China; 2 Laboratory of Oral Tissue Engineering, Fujian Medical University, Fuzhou   350002, Fujian Province, China
  • Online:2010-01-08 Published:2010-01-08
  • Contact: Yan Fu-hua, Professor, Doctoral supervisor, Department of Periodontology, Affiliated Stomatological Hospital, Fujian Medical University, Fuzhou 350002, Fujian Province, China; Laboratory of Oral Tissue Engineering, Fujian Medical University, Fuzhou 350002, Fujian Province, China fhyan2005@126.com
  • About author: Lin Min-kui★, Master, Associate chief physician, Department of Periodontology, Affiliated Stomatological Hospital, Fujian Medical University, Fuzhou 350002, Fujian Province, China; Laboratory of Oral Tissue Engineering, Fujian Medical University, Fuzhou 350002, Fujian Province, China linmk105@yahoo.com.cn
  • Supported by:

    Fujian Provincial Education Bureau (Funding Provincial University) Project, No.2008F5023*; Fujian Provincial Education Bureau Project, No.JA04207*; Fujian Provincial Health Bureau Youth Fund Project, No.2008-1-40*

PDF

414

摘要：

背景：富血小板血浆生物功能的发挥受多种因素的控制，如个体差异、富血小板血浆的使用浓度、富血小板血浆载体、富血小板血浆激活方式等均可影响富血小板血浆的作用。
目的：观察不同浓度凝血酶对富血小板血浆修复颅骨缺损的影响。
方法：抽取兔耳中央动脉全血制备富血小板血浆，稀释富血小板血浆，使其中血小板最终计凝数约为全血的5倍。在16只新西兰兔颅骨顶部分别制备4个直径为8 mm的全厚层骨缺损，按随机数字表法将60 U/mL凝血酶+富血小板血浆+β-磷酸三钙、1 000 U/mL凝血酶+富血小板血浆+β-磷酸三钙、富血小板血浆+β-磷酸三钙与单纯β-磷酸三钙植入4个缺损区域内。术后1，3个月X射线、光镜观察颅骨修复情况，计算各组新骨生成面积百分数。
结果与结论：术后1个月，各组缺损边缘较清晰，缺损周边有不同程度新骨生成，β-磷酸三钙颗粒部分降解，降解处由新骨代替，仅见少量骨陷窝，缺损中心β-磷酸三钙周围可见纤维包绕，仅少数标本可见新骨生成；X射线显示边界清晰，缺损区密度较为均一；各富血小板血浆组新骨生成百分数均高于β-磷酸三钙组(P < 0.05)，但富血小板血浆各组间差异无显著性意义(P > 0.05)。术后3个月，各组缺损边界不清，新骨生成量增加，缺损周边β-磷酸三钙颗粒部分或全部降解并由新生骨所代替，部分区域出现骨小梁，新生骨中骨陷窝增多，多数标本缺损中央有新骨生成；X射线显示各组缺损边缘模糊不清，缺损周边部分密度高于缺损中心部位，与其他正常部位骨密度相当；各富血小板血浆组新骨生成百分数亦明显高于β-磷酸三钙组(P < 0.05)，富血小板血浆+β-磷酸三钙组高于其他3组(P < 0.05)，两凝血酶组差异无显著性意义(P > 0.05)。提示富血小板血浆可促进新西兰兔颅骨缺损修复，60，1 000 U/mL凝血酶对富血小板血浆修复新西兰兔颅骨缺损的作用无影响，可能与并未找到凝血酶的最佳浓度有关。

关键词: 富血小板血浆, 凝血酶, 颅骨缺损, 骨修复, 骨组织工程

Abstract:

BACKGROUND: The biological functions of platelet-rich plasma (PRP) are affected by multiple factors, such as individual difference, PRP concentration, PRP carrier, PRP-activated methods and so on.
OBJECTIVE: To evaluate the effect of PRP, activated by different concentrations of thrombin, on the repair of cranial defects.
METHODS: Whole blood of the central artery of rabbit ears was extracted to prepare PRP, which was then diluted so that the final platelet count was about 5 times of the whole blood. Four whole-thickness layer of cranial defects at an 8-mm diameter were created in 16 New Zealand rabbits and randomly grafted with β-tricalcium phosphate (β-TCP) and PRP, activated by 60 U/mL thrombin; β-TCP and PRP, activated by 1 000 U/mL thrombin; β-TCP and PRP; β-TCP alone. At 1 and 3 months following implantation, X-ray analysis and microscopic observation were performed to onserve cranial repair, the area percent of new bone formation was calculated.
RESULTS AND CONCLUSION: At one month post-surgery, the edge of defects was clear in each group, with varying degrees of new bone formation surrounding the defects, β-TCP particles partially degraded and the degradation lesion was replaced by new bone, only a small amount of bone lacunae was seen, fiber wrapped around the defect center β-TCP, only a small number of specimens showed new bone formation; X-ray showed a clear boundary and uniform defect density; the percentage of new bone formation in the PRP groups were higher than β-TCP groups (P < 0.05). However, there was no significant difference between PRP group groups (P > 0.05). At 3 months post-surgery, the defect boundary was unclear in each group, the new bone formation increased, the β-TCP particles surrounding defects partially or all degraded and were replaced by new bones, some regions appeared trabecular bone, bone lacuna in new bone was increased, the central defect of the majority of specimens exhibited new bone formation; X-ray showed defect boundary was unclear in each group, defect surrounding density was higher than the center defect, and bone mineral density was equivalent to other normal parts; the percentage of new bone formation in the PRP groups was significantly higher than that in the β-TCP groups (P < 0.05), PRP + β-TCP group was higher than the other 3 groups (P < 0.05), there was no significant difference between two thrombin groups (P > 0.05) . It is indicated that although PRP improves the repair of cranial defects, 60 and 1 000 U/mL of thrombin has no effects on PRP repairing cranial defects in New Zealand white rabbits, compared with PRP+β-TCP group, possible the absence of the optimal concentration of thrombin.

中图分类号: