中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (2): 205-208.doi: 10.3969/j.issn.1673-8225.2010.02.004

• 骨组织构建 bone tissue construction • 上一篇    下一篇

硝酸镓对雌激素缺乏性骨质疏松大鼠胶原和骨钙蛋白的影响　 

郭玉霞1，杨大志2，王  多2，镇万新2，刘洪涛2，高国勇2   

  1. 暨南大学第二临床医学院(深圳市人民医院)，1妇科，2脊柱外科，广东省深圳市 518020
  • 出版日期:2010-01-08 发布日期:2010-01-08
  • 作者简介:郭玉霞，女，1962年生，山西省沁水县人，汉族，1983年长治医学院毕业，副主任医师，主要从事妇科内分泌疾病研究。 yangdazhi@medmail.com.cn

Effects of gallium nitrate on collagen and bone calcium protein in rat models of estrogen deficiency-induced osteoporosis

Guo Yu-xia1, Yang Da-zhi2, Wang Duo2, Zhen Wan-xin2, Liu Hong-tao2, Gao Guo-yong2   

  1. 1 Department of Gynecology, 2 Department of Spinal Surgery, Second Clinical Medical College of Jinan University (Shenzhen People’ Hospital), Shenzhen   518020, Guangdong Province, China
  • Online:2010-01-08 Published:2010-01-08
  • About author:Guo Yu-xia, Associate chief physician, Department of Gynecology, Second Clinical Medical College of Jinan University (Shenzhen People’ Hospital), Shenzhen 518020, Guangdong Province, China yangdazhi@medmail.com.cn
  • Supported by:

    Scientific Research Fund of Shenzhen City Science and Technology Bureau in 2004, No. 200405005*

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摘要：

背景：镓是一种人体内非必需微量元素。体内实验已证实，镓可直接抑制骨溶解, 阻止骨钙释放, 增加骨中钙含量，作为一种新的治疗代谢性骨病的药物，其抗骨转化机制尚不清楚。
目的：观察硝酸镓对骨质疏松大鼠胶原和骨钙蛋白的影响。
方法：雌性SD大鼠90只随机数字表法分为2组：对照组(n=20)，显露双侧卵巢后缝合关闭腹腔；骨质疏松组(n=70)，切除双侧卵巢建立骨质疏松大鼠模型。两组各随机取8只采集血、骨标本观察雌激素缺乏性骨质疏松模型复制情况。造模后骨质疏松组(n=62)随机数字表法分为4组：骨质疏松对照组(n=16)生理盐水腹腔注射，3次/周；低剂量镓盐组(n=16)腹腔注射硝酸镓，3次/周；高剂量镓盐组(n=15)按2 mg/kg腹腔注射硝酸镓，3次/周；雌激素组(n=15)腹腔注射雌二醇，3次/周。治疗12周后，检测骨标本中骨胶原、骨钙蛋白和羟脯氨酸水平。
结果与结论：与对照组比较，骨质疏松对照组胶原含量降低(P < 0.05)，氨基己糖和羟脯氨酸含量升高(P < 0.05)，硫酸基差异无显著性意义。经镓盐及雌激素治疗后，胶原含量升高(P < 0.05)，氨基己糖和羟脯氨酸含量降低(P < 0.05)。高剂量镓盐组较低剂量镓盐组效果明显(P < 0.05)，与雌激素组效果相当(P > 0.05)。证实硝酸镓通过提高胶原含量，降低羟脯氨酸含量改善骨质疏松症骨代谢状态，2 mg/kg硝酸镓可取得类似雌激素治疗效果。

关键词: 骨质疏松, 胶原, 骨钙蛋白, 硝酸镓, 骨组织工程

Abstract:

BACKGROUND: Gallium is a non-essential trace element in the human body. In vivo experiments have confirmed that gallium can directly inhibit bone osteolysis, prevent bone calcium release, increase bone calcium content, serves as a new drug treatment of metabolic bone disease, its anti-bone transformation mechanism remains unclear.
OBJECTIVE: To observe the effects of gallium nitrate on collagen and bone calcium protein in osteoporotic rat model.
METHODS: Ninety female SD rats were divided into control group (n = 20) and osteoporosis group (n = 70) at random. Control group rats were sutured to close abdominal cavity after bilateral ovarian was exposed. Osteoporosis group rats received the bilateral ovariectomy to produce osteoporotic rat models, which then were assigned into 4 groups by random digits table: osteoporotic control group (n = 16) by intraperitoneal injection of saline, 3 times per week; Low-dose gallium salt group (n = 16) by intraperitoneal injection of 1 mg/kg of gallium nitrate, 3 times per week; High-dose gallium salt group (n = 15) by intraperitoneal injection of 2 mg/kg of gallium nitrate, 3 times per week; Estrogen group (n = 15) by intraperitoneal injection of estradiol, 3 times per week. After 12 weeks of the treatment, the bone collagen, osteocalcin protein and hydroxyproline levels in bone specimens were detected.
RESULTS AND CONCLUSION: Compared with control group, the content of collagen in osteoporosis control group was reduced (P < 0.05), the contents of aminohexose and hydroxyproline increased (P < 0.05), no significant differences were observed in the content of sulfate-base for both groups. Following gallium and estradiol treatment, the collagen contents enhanced (P < 0.05), while the contents of aminohexose and hydroxyproline reduced (P < 0.05). High-dose gallium salt group had a remarkable curative effect compared with low-dose gallium salt group (P < 0.05), and was similar to estradiol group (P > 0.05). It is indicated that gallium nitrate can improve bone metabolism status with osteoporosis through increasing the content of collagen and decreasing the content of hydroxyproline, 2 mg/kg gallium nitrate are similar to estrogen treatment.

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