中国组织工程研究

中国组织工程研究

• 干细胞培养与分化 • 上一篇    下一篇

人乳腺病变组织乳腺癌耐药蛋白及细胞角蛋白8和嗜铬蛋白A的表达
可能与多向分化潜能干细胞相关？

陈颖欣，李连宏，孙 杰，王 波，王丽霞   

  1. 大连医科大学中日临床病理中心，辽宁省大连市 116027
  • 出版日期:2010-01-04 发布日期:2010-01-04
  • 通讯作者: 李连宏，博士，教授，大连医科大学中日临床病理中心，辽宁省大连市 116027 lilianhong@dlmedu.edu.cn
  • 作者简介:陈颖欣☆，女，1975年生，辽宁省大连市人，汉族，大连医科大学在读博士，主治医师，主要从事乳腺癌干细胞方面的研究。 cyx156@163.com

Expressions of breast cancer resistance protein, cytokeratin-8, and chromogranin-A in human breast carcinoma tissue: Is there a correlation with multi-lineage potential of bone marrow mesenchymal stem cells?

Chen Ying-xin, Li Lian-hong, Sun Jie, Wang Bo, Wang Li-xia   

  1. Center of China-Japan Clinical Pathology, Dalian Medical University, Dalian   116027, Liaoning Province, China
  • Online:2010-01-04 Published:2010-01-04
  • Contact: Li Lian-hong, Doctor, Professor, Center of China-Japan Clinical Pathology, Dalian Medical University, Dalian 116027, Liaoning Province, China lilianhong@dlmedu.edu.cn
  • About author:Chen Ying-xin☆, Studying for doctorate, Attending physician, Center of China-Japan Clinical Pathology, Dalian Medical University, Dalian 116027, Liaoning Province, China cyx156@163.com

PDF

463

摘要：

背景：多数学者认为正常乳腺组织中无神经内分泌细胞，乳腺病变后出现神经内分泌细胞是乳腺上皮干细胞分化过程中受局部微环境和激素水平影响所发生的突变、异常分化结果。

目的：通过检测人乳腺病变组织中乳腺癌耐药蛋白、细胞角蛋白8及嗜铬蛋白A的表达，从干细胞多向分化的角度探讨人乳腺病变中出现神经内分泌细胞的可能机制。

方法：用乳腺癌耐药蛋白作为SP干细胞标记物，用细胞角蛋白8作为腺上皮分化标记物，用嗜铬蛋白A作为神经内分泌分化指标标记物，采用免疫组化方法分别检测这3种蛋白在89例人乳腺组织中的表达情况，并分析3者间的相关性。

结果与结论：乳腺癌耐药蛋白与细胞角蛋白8在正常乳腺组织、乳腺增生组织、乳腺病变组织中均有表达，乳腺癌耐药蛋白在病变组织中的表达呈上升趋势，细胞角蛋白8的表达则随乳腺组织异常分化程度的降低呈逐渐减少，嗜铬蛋白A只在乳腺病变组织中有表达。在正常乳腺组织、乳腺增生组织和乳腺病变组织中，乳腺癌耐药蛋白与嗜铬蛋白A的阳性表达呈显著相关(P < 0.01)，与细胞角蛋白8的阳性表达无明显相关(P=0.069)。上述结果表明正常及增生乳腺组织中未发现神经内分泌细胞，乳腺病变组织中发现神经内分泌细胞，其机制可能与多向分化潜能干细胞的分化有关。

关键词: 乳腺, 乳腺癌耐药蛋白, 细胞角蛋白8, 嗜铬蛋白A, 干细胞

Abstract:

BACKGROUND: Generally speaking, neuroendocrine cells have been not observed in normal breast tissue but found in breast carcinoma tissue which was affected by local microenvironment and hormone level during differentiation of breast epithelial stem cells.

OBJECTIVE: By detecting expressions of breast cancer resistance protein (BCRP), cytokeratin-8 (CK8), and chromogranin-A (CgA) in breast carcinoma tissue, to explore the possible mechanism of neuroendocrine cells observed in breast carcinoma tissue during differentiation of multi-lineage potential of bone marrow mesenchymal stem cells.

METHODS: BCRP, CK8, and CgA were used as markers for SP stem cells, glandular epithelium differentiation, and neuroendocrine differentiation, respectively. Immunohistochemistry was used to detect the expressions of BCRP, CK8, and CgA in breast tissues of 89 subjects and analyze their correlation.

RESULTS AND CONCLUSION: Both BCRP and CK8 expressions were observed in normal breast tissues, hyperplastic tissues, and breast carcinoma tissue. BCRP expression was increased in the breast carcinoma tissue; CK8 expression was decreased with the abnormal differentiation of breast tissue; CgA expression was only detected in breast carcinoma tissue. BCRP expression was significantly correlated with positive CgA expression (P < 0.01), but it was no correlation with positive CK8 expression in normal breast tissues, hyperplastic tissues, and breast carcinoma tissue (P=0.069). The results suggested that neuroendocrine cells were not observed in both normal breast tissues and hyperplastic tissues but in breast carcinoma tissue, which possibly correlated to differentiation of multi-lineage potential of bone marrow mesenchymal stem cells.

中图分类号: