中国组织工程研究

中国组织工程研究 ›› 2022, Vol. 26 ›› Issue (17): 2667-2672.doi: 10.12307/2022.532

• 口腔组织构建 oral tissue construction • 上一篇    下一篇

构建牙周炎伴咬合创伤小鼠模型

杨  沁，王  敏   

  1. 口腔疾病研究国家重点实验室，国家口腔疾病临床研究中心，四川大学华西口腔医院修复科，四川省成都市   610041
  • 收稿日期:2020-11-07 修回日期:2020-12-14 接受日期:2021-09-30 出版日期:2022-06-18 发布日期:2021-12-24
  • 通讯作者: 王敏，博士，教授，口腔疾病研究国家重点实验室，国家口腔疾病临床研究中心，四川大学华西口腔医院修复科，四川省成都市 610041
  • 作者简介:杨沁，女，1995年生，四川省乐山市人，汉族，2018年西南医科大学毕业，医师，主要从事牙周炎、自噬相关的研究。
  • 基金资助:
    四川省区域创新合作项目(2020YFQ0008)，项目参与人：王敏；四川省科技计划项目(2020YFS0174，2019YFS0359)，项目参与人：王敏

Constructing a mouse model of periodontitis with occlusal trauma

Yang Qin, Wang Min   

  1. State Key Laboratory of Oral Diseases, National Oral Disease Clinical Research Center, Department of Prosthodontics, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, Sichuan Province, China
  • Received:2020-11-07 Revised:2020-12-14 Accepted:2021-09-30 Online:2022-06-18 Published:2021-12-24
  • Contact: Wang Min, MD, Professor, State Key Laboratory of Oral Diseases, National Oral Disease Clinical Research Center, Department of Prosthodontics, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, Sichuan Province, China
  • About author:Yang Qin, Physician, State Key Laboratory of Oral Diseases, National Oral Disease Clinical Research Center, Department of Prosthodontics, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, Sichuan Province, China
  • Supported by:
    Regional Innovation Cooperation Project of Sichuan Province, No. 2020YFQ0008 (to WM [project participant]); Sichuan Provincial Science and Technology Programs, Nos. 2020YFS0174 and 2019YFS0359 (both to WM [project participant])

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摘要：

文题释义：
咬合创伤：主要指过大咬合力引起的牙周组织(包括牙周膜、牙槽骨和牙骨质)损伤。
牙周炎：是指一种细菌性感染性疾病，发生在牙周支持组织，其始动因子是菌斑生物膜，菌斑生物膜与宿主的免疫炎症反应均在牙周炎的发生发展中发挥着至关重要的作用。

背景：建立牙周炎伴咬合创伤模型是探究咬合创伤在牙周炎症中所起作用的必要条件，但目前仍没有一种建立牙周炎伴咬合创伤动物模型的稳定可靠的方法。
目的：构建一种高效简便稳定可靠的牙周炎伴咬合创伤动物模型。
方法：对BALB/c小鼠通过复合树脂粘接、口腔植入牙龈卟啉单胞菌、复合树脂粘接+口腔植入牙龈卟啉单胞菌构建单纯咬合创伤、单纯牙周炎、牙周炎伴咬合创伤的动物模型。以健康小鼠为对照组，通过Micro-CT扫描观察小鼠牙槽骨吸收情况，实时荧光定量PCR检测牙槽骨中肿瘤坏死因子α、白细胞介素1β和白细胞介素6的表达，判断是否成功建立牙周炎伴咬合创伤实验动物模型。
结果与结论：①与对照组相比，单纯咬合创伤组小鼠牙槽骨吸收程度较轻，而单纯牙周炎组和牙周炎伴咬合创伤组小鼠牙槽骨吸收加重，且后者大于前者；②与对照组相比，单纯咬合创伤组肿瘤坏死因子α和白细胞介素1β的mRNA相对表达量均明显增高(P < 0.01)，单纯牙周炎组和牙周炎伴咬合创伤组肿瘤坏死因子α、白细胞介素1β、白细胞介素6的mRNA相对表达量均上调(P < 0.01)，且后者上调程度大于前者(P < 0.01)；③结果表明，实验成功建立了牙周炎伴咬合创伤动物模型。

https://orcid.org/0000-0002-0858-750X (杨沁) 

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

关键词: 咬合创伤, 牙周炎, 动物模型, 骨吸收, 炎症反应, 牙槽骨, 小鼠

Abstract: BACKGROUND: Although it is essential to establish a model of periodontitis with occlusal trauma for exploring the role of occlusal trauma in periodontitis, there is yet no stable and reliable method to establish an animal model of periodontitis with occlusal trauma.
OBJECTIVE: To establish a stable, reliable and efficient animal model of periodontitis with occlusal trauma.
METHODS: Animal models of occlusal trauma, periodontitis, and periodontitis with occlusal trauma were made in BALB/c mice by composite resin bonding, oral implantation of Porphyromonas gingivalis, and their combination. Healthy mice acted as control group. Micro-CT scan was used to detect alveolar bone resorption and fluorescent quantitative PCR was used to detect the expression of tumor necrosis factor α, interleukin 1β, and interleukin 6 in the alveolar bone, in order to determine whether the animal model of periodontitis with occlusal trauma was successfully established.  
RESULTS AND CONCLUSION: Compared with the control group, there was no obvious alveolar bone resorption in the simple occlusal trauma group, while the alveolar bone resorption was aggravated in the simple periodontitis group and the periodontitis with occlusal trauma group, especially in the latter group. Compared with the control group, the relative mRNA expression of tumor necrosis factor α and interleukin 1β was significantly increased in the simple occlusal trauma group (P < 0.01). Compared with control group, the expression levels of tumor necrosis factor α, interleukin 1β, and interleukin 6 mRNA were significantly up-regulated in the simple periodontitis group and the periodontitis with occlusal trauma group, and moreover, these expression levels were higher in the periodontitis with occlusal trauma group than the simple periodontitis group (P < 0.01). All these suggest that the animal model of periodontitis with occlusal trauma is successfully established.

Key words: occlusal trauma, periodontitis, animal model, bone resorption, inflammation, alveolar bone, mouse

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