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    13 August 2014, Volume 18 Issue 33 Previous Issue    Next Issue
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    Alteration of biological and immunological properties of aortic homografts after de-endothelialization and decellularization
    Jing Hui, Chang Qing
    2014, 18 (33):  5249-5256.  doi: 10.3969/j.issn.2095-4344.2014.33.001
    Abstract ( 276 )   PDF (1007KB) ( 501 )   Save

    BACKGROUND: Aortic root homograft has been widely used, but it still has a high incidence of calcification and degeneration after operation.
    OBJECTIVE: To investigate the alteration of biological and immunological properties of rabbit aortic root homograft treated with de-endothelialization and decellularization methods, and to find a better decellularization method.
    METHODS: Aortic root homografts obtained from rabbits were randomly divided into fresh group, de-endothelialization group, and decellularization group. The morphology of homografts was evaluated by hematoxylin-eosin, Masson and VG stainings. The biological properties such as thickness, tensile strength, broken extension rate, and heat shrinkage temperature were detected. The immunological features (inflammatory cell infiltration, calcium content and CD152 expression) were determined with immunohistochemistry and flame atomic absorption spectrometry.
    RESULTS AND CONCLUSION: As demonstrated in morphological observation, protocols of de-endothelialization and decellularization methods we used attained our desired aim of decellularization perfectly. Tensile strength and broken extension rate of decellularization group were decreased significantly compared with fresh group (P < 0.01), while the difference between de-endothelialization group and fresh group was not significant (P > 0.05). Hematoxylin-eosin staining showed that, inflammatory reaction was the most apparent in fresh group, showing a large amount of inflammatory cells infiltration; compared with fresh group, inflammatory reaction was significantly attenuated in de-endothelialization group, the number of inflammatory cells was significantly decreased. The inflammatory reaction was the lowest in the decellularization group, only inflammatory cells infiltrated the tissue edge. Immunohistochemistry showed that, CD152 expression was significantly increased in de-endothelialization and decellularization groups compared with fresh group (P < 0.05). Calcium content in de-endothelialization and decellularization groups was significantly decreased compared with fresh group (P < 0.05). It could be concluded that the immunogenicity of de-endothelialized and decellularized aortic homografts is decreased significantly, and the de-endothelialization method could better maintain the tensile property.



    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Endoplasmic reticulum stress-induced apoptosis of osteoblasts within the osteolytic craniums
    Liu Guo-yin, Wang Rui, Dong Lei, Zhang Jun-feng, Zhao Jian-ning
    2014, 18 (33):  5257-5265.  doi: 10.3969/j.issn.2095-4344.2014.33.002
    Abstract ( 396 )   PDF (3599KB) ( 591 )   Save

    BACKGROUND: Wear particles-induced osteoblasts apoptosis in vitro has been documented in many studies. However, the apoptosis of osteoblasts in osteolytic bone tissue and the selective mechanisms involved in the pathogenesis of osteolysis have been studied rarely.
    OBJECTIVE: To investigate the influence of endoplasmic reticulum (ER) stress on the apoptosis of osteoblasts in osteolytic bone tissue and osteolysis progression.
    METHODS: The mouse model of osteolysis was induced with wear particles placed onto the calvaria. The experiment was divided into four groups: blank control group (PBS stimulation); wear particle group (nano-alloy powder suspension stimulation); ER stress positive control group (nano-alloy powder + thapsin stimulation); and ER stress inhibitor group (nano-alloy powder + sodium 4-phenylbutyrate stimulation). The histopathologic change
    of osteolysis was assessed by hematoxylin-eosin, toluidine blue and alkaline phosphatase staining. Osteoblast proliferation and differentiation in osteolytic craniums were measured. The expression of ER stress markers in osteolytic craniums was examined by western blot analysis. Osteoblast apoptosis was analyzed by TUNEL staining and immunohistochemistry of Caspase-3 in osteolytic craniums.
    RESULTS AND CONCLUSION: Wear particles were capable of inducing osteolysis, aggravating the infiltration of inflammatory cells, and inhibiting the differentiation of osteoblasts in osteolytic craniums. Meanwhile wear particles upregulated the ER stress markers and promote the apoptosis in osteolytic craniums. Blocking ER stress with sodium 4-phenylbutyrate dramatically reduced the severity of osteolysis, significantly reduced bone invasion and inflammatory infiltration, promoted the differentiation of osteoblasts, and dramatically reduced the apoptosis. Along with apoptosis, the expression of ER stress marker was decreased. The present study suggests that the ER stress may be crucial for osteolysis and represent a potential therapeutic target in the prevention and treatment of patients with total joint replacement who are at high risk of early aseptic loosening development.



    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Comparison of three morphology methods for observing mineralization nodules of osteoblasts
    Liao Nai-shun, Li Zuan-fang, Lin Ru-hui, Chen Wen-lie, Huang Yun-mei
    2014, 18 (33):  5266-5270.  doi: 10.3969/j.issn.2095-4344.2014.33.003
    Abstract ( 670 )   PDF (1999KB) ( 1537 )   Save

    BACKGROUND: Mineralized nodules are the mature marker of osteoblast differentiation, and the observation methods mainly use alizarin red staining.
    OBJECTIVE: To compare the observation results of mineralized nodules by three methods, and to explore their characteristics and advantages, as well as further application in the research of bone disease.
    METHODS: The rat osteoblast-like cell line UMR-106 were cultured in the fresh medium that was changed every day, for 14 days. Alizarin red staining-light microscope, tetracycline fluorescence labeling-laser confocal scanning microscopy, and scanning electron microscopy were used to observe mineralized nodules. The calcium content of mineralized nodules was quantified using scanning electron microscopy and energy dispersive spectroscopy. In addition, tumor necrosis factor alpha that could inhibit the proliferation and differentiation of osteoblasts was used as the control.
     RESULTS AND CONCLUSION: The three morphology methods could be used to observe the mineralized nodules of normal osteoblasts. As for tumor necrosis factor alpha, no mineralized nodules of osteoblasts were observed by alizarin red staining-light microscopy; small mineralized nodules were observed by tetracycline staining-laser scanning confocal microscopy and scanning electron microscopy, suggesting tetracycline staining and scanning electron microscopy were more sensitive in the observation. Scanning electron microscopy could be used to observe the submicroscopic structures of mineralized nodules in the osteoblasts, and the formation of mineralized nodules, including the calcium secretion. Additionally, scanning electron microscopy combined with energy dispersive spectroscopy analysis can successfully quantify and position the mineralized nodules, indicating a potential application in the research of bone diseases.



    中国组织工程研究
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    Construction of the central vascularized bone
    Wang Si-ming, Kan Na, Zhang Lei
    2014, 18 (33):  5271-5275.  doi: 10.3969/j.issn.2095-4344.2014.33.004
    Abstract ( 369 )   PDF (1909KB) ( 477 )   Save

    BACKGROUND: The vascularization plays a key role in bone formation and reconstruction. Bioactive β-tricalcium phosphate has a porosity and absorbability that allow a possibility of constructing vascularized bone.
    OBJECTIVE: To explore the blood supply and construction of the central vascularized β-tricalcium phosphate, as well as the mechanism of vascularization in artificial bone for its further clinical application.
    METHODS: The lumbar dorsal artery of New Zealand rabbits was isolated and prepared into vascular bundle. At the experimental side, the vascular bundle was implanted into channel of the β-tricalcium phosphate, then filled with the autologous tiny bone particles, and embedded into the latissimus dorsi muscle. Animals without the vascular bundle implantation were taken as the control group. The samples were determined for the morphology and histology at 4, 8, and 12 weeks post-surgery.
    RESULTS AND CONCLUSION: The site where the vascular bundle was implanted appeared foramen nutriens-like structure, the blood vessels were abundant and smooth. The central vascularized tricalcium phosphate were filled with new-born vessels at 4-8 weeks. The newly formed bone and bone metabolism appeared at 4 weeks and newly formed bones were more mature at 12 weeks. In the control group, there were only a few new-born vessels, the newly formed bone was immature. The central vascularized β-tricalcium phosphate can significantly improve the vascularization of artificial bone. This model can probably serve as a new method for tricalcium phosphate in clinical use.



    中国组织工程研究
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    Granulocyte colony-stimulating factor induces osteogenesis
    Zhang Bin, Lv Song-cen, Tao Shu-qing, Rong Jie-sheng
    2014, 18 (33):  5276-5281.  doi: 10.3969/j.issn.2095-4344.2014.33.005
    Abstract ( 279 )   PDF (2256KB) ( 357 )   Save

    BACKGROUND: Mesenchymal stem cells are a kind of adult stem cells with the potentials of self-replicating and multi-directional differentiation. Under certain conditions, the cells can develop into bone, cartilage and other types of cells. Granulocyte colony-stimulating factors can promote regeneration of mesenchymal stem cells.
    OBJECTIVE: To observe the osteogenic induction effect of granulocyte colony-stimulating factors by animal experiment, and provide a better treatment method for clinical treatment of bone defect.
    METHODS: Forty New Zealand rabbits were randomly divided into treatment group and model group. All animals were removed 2 cm length of the right radial bone and the surrounding periosteum, and then implanted with demineralized bone matrix into the defect. The treatment group was subcutaneously injected with granulocyte colony-stimulating factor 30 μg/kg per day, the injection was started at preoperative 3 days and given for 7 continuous days. In control group, the same dose of physiological saline was injected.
    RESULTS AND CONCLUSION: In the treatment group, the number of mesenchymal stem cells, the quantity and quality of bone formation at bone defects were better than those in the model group. The experimental findings indicate that granulocyte colony-stimulating factor can obviously increase the number of mesenchymal stem cells and promote bone formation at the area of bone defect, which can contribute to early repair of bone defects.



    中国组织工程研究
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    Effects of Wallerian degeneration on biological characteristics and secretory function of Schwann cells in rats with sciatic nerve injury
    Li Yue-zhen, Wu Geng, Wu Yang, Jin Xiu-dong, Zhang Ji-fei, Zhao Fu-sheng
    2014, 18 (33):  5282-5287.  doi: 10.3969/j.issn.2095-4344.2014.33.006
    Abstract ( 331 )   PDF (1986KB) ( 454 )   Save

    BACKGROUND: Studies have shown that Schwann cells form a Bunger band in the basement tube and guide the extension of regenerating axons after peripheral nerve injury, but the exact mechanism remains to be explored.
    OBJECTIVE: To explore the effect of Wallerian degeneration on biological characteristics and secretory function of Schwann cells in rats with sciatic nerve injury.
    METHODS: A rat model of sciatic nerve injury was established and divided into two groups: sciatic nerve transection group and surgical control group. Schwann cells were isolated and cultured from sciatic nerve segments by one enzyme digestion. The cell morphology was observed under light microscope and S-100 protein expression was determined by immunofluorescence staining. After subculture, the first generation of Schwann cells were chosen to draw the growth curve by the counting method within 14 days. The cell activity was detected by MTT assay. The adhesion of Schwann cells was examined by acid phosphatase analysis and the concentration of nerve growth factor was detected by ELISA method.
    RESULTS AND CONCLUSION: At 14 days after primary culture, a great number of Schwann cells were observed near the edges of nerve segments in the sciatic nerve transection group, but only small number of Schwann cells scattered around nerve segments in the control group. Schwann cells in both groups showed S-100 positive expression. At 3 days after subculture, Schwann cells reached the logarithm proliferative phase, the cell number and proliferation absorbance values in both groups were increased along with time extension. Furthermore, the number of Schwann cells and absorbance value in the sciatic nerve transection group were significantly higher than those of control group (P < 0.05). The adhesion ability in the sciatic nerve transection group was also significantly higher than those in the control group (P < 0.05). ELISA results showed that, the concentrations of nerve growth factor in the sciatic nerve transection group were significantly higher than those in the control group at 4, 6, 8, 10, 12 and 14 days (P < 0.05). After sciatic nerve injury, Wallerian degeneration can induce Schwann cells dedifferentiate into the precursors, significantly influence the biological function of Schwann cells, promote the proliferation of Schwann cells within the short term, secrete large amounts of neurotrophic factors, enhance cell adhesion, and provide a suitable microenvironment for regenerated axons. In addition, it creates the necessary microenvironment for peripheral nerve regeneration.



    中国组织工程研究
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    Ultrastructure of periosteal cells induced by bone morphogenetic protein 7 in vitro
    Liao Jia-cheng, Bei Kang-sheng, Lian Yin-chuan, Xu Jin-wen, Liu Yan-xiao, Huang Xiao-yu
    2014, 18 (33):  5288-5292.  doi: 10.3969/j.issn.2095-4344.2014.33.007
    Abstract ( 419 )   PDF (2063KB) ( 372 )   Save

    BACKGROUND: Study confirms that bone morphogenetic protein can induce osteogenesis; however the ultrastructure of periosteal cells induced by bone morphogenetic protein-7 remains poorly reported.
    OBJECTIVE: To study the bioactivity and ultrastructure of periosteal cells induced by bone morphogenetic protein-7 in vitro.
    METHODS: The primary periosteal cells isolated from adult tibial bone were in vitro cultured, and then divided into experimental group and control group. In the experimental group, cells were cultured with bone morphogenetic protein-7 and culture adjuvant; while cells in the control group were only cultured with the adjuvant. Three samples in each group were tested at 5, 10, 15 days, respectively. The general structure of cultured cells was observed using von Kossa staining, and the ultrastructure was observed under transmission electron microscopy.
    RESULTS AND CONCLUSION: The periosteal cells in the two groups grew well in vitro, showing uniform morphology. Early cells were spindle-shaped, with strong three-dimensional sense and full transparency; mitotic cells were short columnar or cubic shaped, there were a lot of rough endoplasmic reticulum and Golgi complex in osteoblasts under electron microscope. Later stage of cells developed from long fusiform into wide shuttle and irregular shape, there were a large number of matrix vesicles within the cells under the electron microscope. The membrane coating, alkaline phosphatase and calcium-binding protein in the cytoplasm, as well as calcium crystals were found. The osteogenesis basement and lateral sides appeared projections, which were connected with adjacent bone cells. Induction of bone morphogenetic protein-7 in vitro promotes the osteoblasts proliferation, division and bone formation speed. The results suggest that bone morphogenetic protein-7 can significantly enhance the proliferation ability of osteoblasts in vitro.



    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Bisphosphonate effects on capthesin K and bone resorption function during osteoclast differentiation  
    Dong Wei, Feng Xiao-jie, Liang Yong-qiang, Peng Hong-feng, Deng Jiu-peng, Wen Li-ming, Qi Meng-chun
    2014, 18 (33):  5293-5298.  doi: 10.3969/j.issn.2095-4344.2014.33.008
    Abstract ( 337 )   PDF (895KB) ( 459 )   Save

    BACKGROUND: Studies have shown that bisphosphonates inhibit osteoclast resorption, but whether cathepsin K, a key cytokine of bone resorption, plays an effect has rarely been reported.
    OBJECTIVE: To study the effect of bisphosphonate on capthesin K and bone resorption function during osteoclast differentiation.
    METHODS: Osteoclasts were cultured by mouse monocyte-macrophage cell line-RAW264.7. The cells were divided into two groups: control group, treated with 100 μg/L receptor activator of nuclear factor κB ligand factor; alendronate group, treated with 100 μg/L receptor activator of nuclear factor κB ligand factor+10-7 mol/L alendronate. Osteoclastogenesis and resorption function of osteoclasts were examined at 7 days of culture and gene expression of capthesin K was detected by immunofluorescence method at 72 hours of culture. Western blot assay was used to detect capthesin K protein expression at 72 hours of culture.
    RESULTS AND CONCLUSION: Tartrate-resistant acid phosphatase positive multinuclear cells were observed and resorption lacunae formed in two groups. Control group showed the higher number of tartrate-resistant acid phosphatase positive multinuclear cells and larger size of resorption lacunae than the alendronate group (P < 0.01). Immunofluorescence showed expression of capthesin K was higher in the control group than the 
    alendronate group (P < 0.01); furthermore, the protein expression of capthesin K was also lower in the alendronate group than the control group (P < 0.01). These findings indicate that bisphosphonates could strongly inhibit osteoclastogenesis and its resorption function by inhibiting gene expression of capthesin K.



    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Development of acetabulum and the content of bone morphogenetic protein 7 after local lesion of cartilago acetabularis in a rabbit model
    Gu Yang-lin, Zhu Guo-xing
    2014, 18 (33):  5299-5304.  doi: 10.3969/j.issn.2095-4344.2014.33.009
    Abstract ( 337 )   PDF (726KB) ( 450 )   Save

    BACKGROUND: In a primate model of ulna defect, exogenous recombinant human bone morphogenetic protein-7 can promote the healing of autologous bone defect.
    OBJECTIVE: To probe into the variation of the development of acetabulum and the content of bone morphogenetic protein 7 after local lesion of cartilage acetabularis in rabbits.
    METHODS: Thirty-six rabbits (4-week-old) were anesthetized to expose the left hip joint by the anterolateral approach. A 1.5 mm×2.0 mm×0.5 mm sample of the cartilago acetabularis was scraped from each rabbit as experimental group; and the right hip joint was also exposed as control group. To evaluate the hip development, X-rays were used at 4, 6, 8 weeks after the operation, 12 rabbits were killed randomly at 4, 6, 8 weeks after operation for general observation, histological examination and immunohistochemical assay.
    RESULTS AND CONCLUSION: By the radiographic examination, the acetabulums became shallow and irregular in morphology. The shape of these femoral heads was applanate. Semiluxation and dislocation could be observed in some cases. Hematoxylin-eosin staining showed the regenerated fibrous tissue in the lesion region gradually migrated from the meager layer to the deep layer to form fibrous cartilage, cartilage cells from  proliferative and hypertrophic layers lost their normal columnar structure and arranged disorderly. Immunohistochemistry detection showed that over time, the dyeing of bone morphogenetic protein 7 became light in color. In the control group, a typical structure of articular hyaline cartilage was observed. The results suggest that damaging the cartilago acetabularis led to developmental hip dysplasia, and the content of bone morphogenetic protein 7 from all regions decreased by the extension of time.



    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Effect of mechanical centrifugal force on Runx-2 mRNA in osteoblasts’ bone morphogenetic protein signal pathway
    Duan Feng, Guan Jian, Yang Hong-yan, Wang Xin-yu, Zhang Guo-liang, Zhu Yang
    2014, 18 (33):  5305-5309.  doi: 10.3969/j.issn.2095-4344.2014.33.010
    Abstract ( 317 )   PDF (605KB) ( 839 )   Save

    BACKGROUND: Mechanical strain certainly has an effect on physiological activities of osteoblasts. Runx-2 is a target of bone morphogenic protein signal and is an important factor for regulation of osteoblastic differentiation. Bone morphogenic protein signal transduction pathway is involved in physiological response of osteoblast to stimulation of mechanical centrifugal force.
    OBJECTIVE: To observe the effect of mechanical centrifugal force on bone morphogenetic protein signal pathway under different time period and speed.
    METHODS: MC3T1-E1 cells were pre-treated in DMEM medium containing 10% fetal bovine serum for 24 hours, and then divided into control group, 90 r/min group, 180 r/min group and 250 r/min group. Each group was then subdivided into 6 hours, 12 hours and 24 hours centrifugation subgroups. Experiments were repeated for three times for different centrifugal speed and different time period. Except centrifugation, the control group was   under the same environment. Total RNA was extracted and reversely transcribed into cDNA. Runx-2 gene expression was determined by real time fluorescent quantitative PCR.
    RESULTS AND CONCLUSION: The expression of Runx-2 mRNA was increased with extension of time, showing a positive correlation between the two. The mRNA expression at 180 r/min was significantly higher than that at 90 r/min and 250 r/min (P < 0.01); at 90 r/min and 180 r/min, the Runx-2 mRNA expression was higher than that in the control group (P=0.039), both of them showed significant difference along with the time. The difference of centrifugal force speed and duration is associated with different physiological response of osteoblasts in bone morphogenic protein signal pathway, which plays an important role in mechanical signal transmission and cascade reaction.



    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Interleukin-10 and conjugative plasmid of Salmonella mediate bacterial biofilm formation
    Que Feng-xia, Liu Zhen, Wang Ting, Yan Jing, Li Yuan-yuan, Wu Shu-yan, Huang Rui
    2014, 18 (33):  5310-5316.  doi: 10.3969/j.issn.2095-4344.2014.33.011
    Abstract ( 349 )   PDF (952KB) ( 336 )   Save

    BACKGROUND: Previous studies discovered that pRST98, originally isolated from Salmonella enterica serovar typhimurium (S.typhimurium) could promote bacterial biofilm formation. In addition, bacterial harboring pRST98 can promote the secretion and expression of interleukin-10 after infection in cells and animals.
    OBJECTIVE: In vitro studies have discovered the effects of interleukin-10 at varying concentrations and conjugative plasmid pRST98 on the biofilm formation of S.typhimurium.
    METHODS: S.typhimurium wild-type strain χ3306, virulence plasmid-deletion S.typhimurium strain χ3337 and pRST98-transconjugant S.typhimurium χ3337/pRST98 were established in vitro and cultured for biofilm formation. 1, 10, 100 µg/L interleukin-10 were added during the biofilm formation. 0 µg/L interleukin-10 was set as a control. Crystal violet staining method, semi-quantitative method, confocal laser scanning microscopy and scanning electron microscopy were used to determine the effects of interleukin-10 on the biofilm formation and compare the effects of S.typhimurium with or without pRST98.
    RESULTS AND CONCLUSION: Intra-group comparison showed that, compared with the control group, S.typhimurium gathered together and formed thicker biofilm in concentration of 1 and 10 µg/L of interleukin-10. The promotion effects of S.typhimurium on biofilm formation were greatly improved in 10 µg/L. Interleukin-10 in 100 µg/L inhibited S.typhimurium biofilm formation. Inter-group comparison showed that, A570 in χ3337/pRST98 was greatly higher than that in χ3306 and χ3337 under the same concentration of interleukin-10. The results indicate that both 1 and 10 µg/L of interleukin-10 promote biofilm formation, especially bacteria harboring pRST98.



    中国组织工程研究
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    Synergistic effect of high mobility group protein B1 on calcium phosphate-induced release of inflammatory cytokines from macrophages 
    Feng You-cai, Deng Yao-liang, Tao Zhi-wei, Wang Xiang, Li Cheng-yang, Huang Peng, Wu Bo
    2014, 18 (33):  5317-5322.  doi: 10.3969/j.issn.2095-4344.2014.33.012
    Abstract ( 347 )   PDF (708KB) ( 331 )   Save

    BACKGROUND: More and more evidence suggests that macrophages and inflammation reactions are involved in the formation and development of nephrolithiasis. Previous studies have found that calculi crystals can stimulate macrophages to release high mobility group protein B1.
    OBJECTIVE: To investigate the synergistic effect of high mobility group protein B1 in calcium phosphate induced release of interleukin-1β, interleukin-6, tumor necrosis factor α and monocyte chemotactic factor 1 from human macrophages.        
    METHODS: (1) The induced U937 cells were respectively stimulated with RPMI (blank), 100 mg/L calcium phosphate, 100 μg/L high mobility group protein B1 and 100 mg/L calcium phosphate+100 μg/L high mobility group protein B1 for 1, 2 and 4 hours to collect cell supernatant. (2) The induced U937 cells were respectively stimulated with 100 mg/L calcium phosphate, 100 mg/L calcium phosphate+10 μg/L high mobility group protein 
    B1, 100 mg/L calcium phosphate+50 μg/L high mobility group protein B1, 100 mg/L calcium phosphate+100 μg/L high mobility group protein B1 for 4 hours to collect cell supernatant. Levels of interleukin-1β, interleukin-6, tumor necrosis factor α and monocyte chemotactic factor 1 were determined by ELISA.
    RESULTS AND CONCLUSION: The levels of interleukin-1β, interleukin-6, tumor necrosis factor α and monocyte chemotactic factor 1 in the cell culture supernatant of 100 mg/L calcium phosphate group and 100 μg/L high mobility group protein B1 group were both higher than those in the blank group in a time-dependent manner (P < 0.05). The levels of interleukin-1β, interleukin-6, tumor necrosis factor α and monocyte chemotactic factor 1 in the cell culture supernatant of different concentrations of high mobility group protein B1 groups were all higher than those in the
    100 mg/L calcium phosphate group in a concentration-dependent manner (P < 0.05). The results suggest that both calcium phosphate and high mobility group protein B1 can induce the release of interleukin-1β, interleukin-6, tumor necrosis factor α and monocyte chemotactic factor 1 from human macrophages and the high mobility group protein B1 has the synergistic effect with calcium phosphate to induce interleukin-1β, interleukin-6, tumor necrosis factor α and monocyte chemotactic factor 1 from human macrophages.



    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Change of motor neurons and skeletal muscles distal to the lesion after spinal cord injury in rats
    Wang Yuan-yuan, Hong Yi, Wang Xue-fei, Cai Qing, Tang He-hu, Li Xiang, Liang Guang-xu, Zhang Jun-wei
    2014, 18 (33):  5323-5328.  doi: 10.3969/j.issn.2095-4344.2014.33.013
    Abstract ( 275 )   PDF (2997KB) ( 451 )   Save

    BACKGROUND: The majority of studies focus on the lesions of spinal cord injury, while little evidence is available on the change of morphology and structure of distal nerve, muscle and motor endplates following spinal cord injury.
    OBJECTIVE: To investigate the time window change of the morphology of motor neurons and skeletal muscles caudal to the lesion after spinal cord injury in rats.
    METHODS: Fifty healthy adult Sprague-Dawley rats were randomly divided into three groups: control group (n=5; without treatment), sham operation group (n=10), and spinal cord injury group (n=35). The sham operated rats only received laminectomy. In the spinal cord injury group, rats were subject to complete T10 spinal cord injury by total laminectomy and cord transverse resection. Then the morphological change including sciatic nerve, motor endplate and median gastrocnemius was observed for each group at 1, 2, 4, 12, 24 weeks after injury.
    RESULTS AND CONCLUSION: (1) The myelin sheath layers of sciatic nerve were separated partially at 4 weeks in rats with spinal cord injury, the myelin sheaths were fragmented with the regeneration of thin-myelinated and unmyelinated axons at 12 weeks. There was a decrease in myelinated axons and an increase in thin-myelinated 
    and unmyelinated axons at 24 weeks. (2) The synaptic gutters of motor endplate, the presynaptic and postsynaptic membrane and synaptic space were distinct at 4 weeks in rats with spinal cord injury, the degenerated motor endplates coexsisted with the intact ones at 12 weeks. The motor endplate disappeared at 24 weeks. (3) There was a slight decrease in muscle cross-sectional area at 2 weeks in rats with spinal cord injury, but no structural change was found, the membrane of myocytes was partially weakened at 4 weeks, the border of myocytes was obscure with hyperplasia of connective tissue at 12 weeks, and myocytes gathered and in fusion at 24 weeks. As natural history of completely transected spinal cord injury in rats, there were significant changes in morphology of peripheral nerve, motor endplate and skeletal muscles caudal to the lesion at 12 weeks, and the changes were destructive at 24 weeks.



    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Subcutaneous embedment of the acellular urethra in rats
    Liu Xiao-yan, Wang Yuan-zheng, Li Ying, Li Lin, Mao Dan, Li Hang-xu
    2014, 18 (33):  5329-5333.  doi: 10.3969/j.issn.2095-4344.2014.33.014
    Abstract ( 329 )   PDF (1795KB) ( 342 )   Save

    BACKGROUND: The nature of graft rejective reaction is the antigen immunological reaction of recipient’s immune system in response to donor’s graft.
    OBJECTIVE: To observe the effect of chemical extraction on the immunogenicity of acellular peripheral urethral isografts.
    METHODS: Twenty urethras of Sprague-Dawley rats, 1 cm long, were included in this study. Ten urethras were treated with chemical acellular method to remove immunogenic component, thus prepared into acellular urethras; another ten urethras were fresh, without any acellular treatment. Acellular urethras were implanted into the back of ten Wistar rats, while fresh urethras into another ten rats. 6 weeks after transplantation, the specimens were harvested for gross and histological observations.
    RESULTS AND CONCLUSION: After acellular or fresh urethras were implanted into the rats, no autotomy was observed, the incision healed well, the diet and activities of the rat were normal, and no ulcer formation was found. At 6 weeks, tissue membrane formed around the acellular urethra, while no formation was visible around fresh urethra by gross observation. Hematoxylin-eosin staining showed that, acellular urethras were gradually absorbed and replaced by smooth muscular tissue. And unequal blood vessels grew in the acellular urethra, even formed blood sinusoid. In the control group, the urethras were not absorbed and no blood vessels were grown. The acellular urethras can gradually form normal urethra in rats, indicating that the acellular urethral autograft has a lower antigenicity and can replace the cavernous body of normal urethra.



    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Effects of proton pump inhibitor FR167356 on osseointegration of dental implant in osteoporosis rabbits
    Zhang Peng, Shi Wen-yi, Guo Da-wei, Yu Jiang-bo, Song Ling, Zhang Chun-yan, Cao Yang
    2014, 18 (33):  5334-5340.  doi: 10.3969/j.issn.2095-4344.2014.33.015
    Abstract ( 333 )   PDF (2779KB) ( 383 )   Save

    BACKGROUND: Vacuolar proton pump on the cytoplasmic membrane of osteoclasts is an essential enzyme for bone histolysis. Vacuolar proton pump inhibitor can significantly inhibit the in vitro cultured osteoclasts.
    OBJECTIVE: To observe the effect of proton pump inhibitor FR167356 on the osseointegration of dental implant of osteoporosis rabbits.
    METHODS: A total of 24 female Japanese white rabbits aged 5 months were randomly divided into three groups: ovariectomy (OVX) group, FR167356 group and shamed operation group. Each group contained eight rabbits. Rabbits of OVX group and FR167356 group received a surgical removal of bilateral ovaries, while rabbits of sham operation group had a surgical removal of equivalent adipose tissue beside the ovaries. Two titatium implants (8 mm long, 3.3 mm diameter) were installed into bilateral proximal tibias respectively 12 weeks after OVX  operation. FR167356 was administrated by muscle injection in FR167356 group; meanwhile equivalent normal saline was administrated in the OVX group and sham operation group. At 4 and 8 weeks after implantation, animals in each group were sacrificed respectively for X-ray imaging, histomorphology, and mechanical test.
    RESULTS AND CONCLUSION: X-ray examination showed that at 4 weeks, the implants in the OVX group exhibited a high resistance projective image, low density image of clearance screw thread, and clear boundaries between bone tissue than the other two group. At 12 weeks, the density of the clearance screw thread in sham operation group and FR167356 group was more closer to the surrounding bone tissue when compared with the OVX group, the boundaries between the implant and the surrounding bone tissue disappeared more apparently, and no significant differences were found between the two groups. Histomorphology observation revealed that, at 4 weeks after implantation, new bone with porous and trabecular extended along the implant surface to the root direction in FR167356 and sham operation groups. There were no significant differences in the two groups. At 12 weeks after implantation, the bone mass around implant was increased greatly and the trabecular grew thicker. This phenomenon was not observed in the OVX group. Mechanic test showed that at 4 and 12 weeks after implantation, the maximum pullout force in the OVX group was significantly lower than that in the other two groups. Local application of FR167356 can significantly improve the osseointegration of the implant in osteoporosis rabbits.



    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Radiographic measurement of bone mineral density combined with vertebral fracture assessment for the improvement of osteoporosis diagnosis
    Cai Si-qing, Yan Li-sheng, Li Yi-zhong, Zhuang Hua-feng, Cai Dong-lu
    2014, 18 (33):  5341-5345.  doi: 10.3969/j.issn.2095-4344.2014.33.016
    Abstract ( 338 )   PDF (645KB) ( 469 )   Save

    BACKGROUND: The diagnosis of osteoporosis depends upon the bone mineral density T-score of ≤ -2.5 standard deviation or brittle fractures. Bone mineral density measurement combined with vertebral fracture assessment might prevent the missed diagnosis of osteoporosis due to bone mineral density evaluation alone, and improve the diagnosis rate of osteoporosis.
    OBJECTIVE: To evaluate the effect of bone mineral density measurements combined with vertebral fracture assessment for the diagnosis of osteoporosis.
    METHODS: Bone mineral density measurements of proximal femur and vertebral fracture assessment for lateral thoraco-lumbar images were consecutively done for 217 postmenopausal women who aged ≥ 50 years. The rate of osteoporosis diagnosed with bone mineral density T score was compared with that diagnosed with bone mineral density combined with vertebral fracture assessment. The effects of bone mineral density on the vertebral fracture were analyzed.
    RESULTS AND CONCLUSION: 92 (42.4%) patients had bone mineral density T score ≤ -2.5, which met the threshold for diagnosis of osteoporosis. 102 (47.0%) patients had osteopanic (-1 > T > -2.5) and 23 (10.6%) had normal range of bone mineral density. 158 patients had no vertebral fractures and 59 (27.2%) patients had 101  vertebral fractures. The vertebral fracture rate was 21.6% in the patients with bone mineral density T > -2.5 and 34.8% in the patients with bone mineral density T ≤ -2.5, with significant differences (P < 0.05). Bone mineral density in combination with vertebral fracture assessment for the diagnosis rate of osteoporosis was up to 54.8%, which was significantly higher than the rate diagnosed with only bone mineral density (12.4%; P=0.01). Bone mineral density measurement combined with vertebral fracture assessment improves the diagnosis of osteoporosis for postmenopausal women.



    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Biostimulation feedback treatment improves surface electromyography value of pelvic floor muscle during contraction in women with mild and moderate stress urinary incontinence
    Feng Ling
    2014, 18 (33):  5346-5350.  doi: 10.3969/j.issn.2095-4344.2014.33.017
    Abstract ( 490 )   PDF (740KB) ( 488 )   Save

    BACKGROUND: Biostimulation feedback treatment has been widely used for female stress urinary incontinence. The treatment principle is based on the electromyographic signal measured and the combination of biofeedback technique and electrotherapy technique.
    OBJECTIVE: To evaluate the effect of biofeedback electrical stimulation in treatment of female stress urinary incontinence.
    METHODS: Thirty female patients suffering from light or moderate stress urinary incontinence received biofeedback electrical stimulation for 4-8 weeks. The stimulation parameters were 60 Hz, 20-60 mA, 12-second feedback. The patients were instructed to contract pelvic floor muscle. The peak value of surface electromyography in pelvic floor muscular 2-second-rapid-contraction, mean value of pelvic floor muscular 10-second-contraction, mean value of the pelvic floor muscular 60-second-tolerance-contraction, and the rate of urine leakage were observed before and after treatment.
    RESULTS AND CONCLUSION: The peak value of surface electromyography in pelvic floor muscular 2-second-rapid-contraction, mean value of pelvic floor muscular 10-second-contraction, and mean value of the pelvic floor muscular 60-second-tolerance-contraction were significantly increased after treatment, compared with before treatment (P < 0.05). The rate of urine leakage was decreased obviously after treatment (P < 0.05). Biofeedback electrical stimulation can significantly increase surface electromyography value of pelvic floor muscles during contraction, and is an effective treatment for female stress urinary incontinence.



    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Limited incision plus autologous tendon graft improve knee joint functions in the treatment of knee ligament injuries
    Cui Zhou-kai, Shen Hai-sheng, Zhao Ya-li
    2014, 18 (33):  5351-5355.  doi: 10.3969/j.issn.2095-4344.2014.33.018
    Abstract ( 337 )   PDF (638KB) ( 537 )   Save

    BACKGROUND: Early diagnosis and accurate treatment of ligament injuries are the premise of avoiding chronic instability of knee joint and promoting functional recovery of knee joint.
    OBJECTIVE: To investigate the clinical efficacy of limited incision with autologous tendon graft for knee ligament injury.
    METHODS: Eighty patients with knee ligament damage were randomly divided into two groups. The cases of control group were given orthosis treatment, and the cases of observation group were treated with the limited incision plus autologous tendon graft.
    RESULTS AND CONCLUSION: At 6 months post-treatment, the excellent efficacy rate and postoperative quality of life score were increased in the observation group, compared with the control group (P < 0.05). The active and passive ranges of motion of knee joint in the observation group were significantly lower than that in the control group (P < 0.05). At 3 months post-treatment, Lysholmp scores of the observation group was significantly lower than that in the control group (P < 0.05). Limited incision plus autologous tendon graft treatment can significantly improve knee function after knee ligament injury.



    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Mouse nerve growth factor injection via different ways for treatment of peripheral nerve injury
    Chen Qing-zhen, Shi Ming-xiang, Liu Sheng-fei, Du Lan-xiang, Li Jiang-qun
    2014, 18 (33):  5356-5360.  doi: 10.3969/j.issn.2095-4344.2014.33.019
    Abstract ( 356 )   PDF (577KB) ( 478 )   Save

    BACKGROUND: Mouse nerve growth factor can promote the repair and regeneration of injured nerves, but current experimental research shows that the effects of different treatment methods are still controversial.
    OBJECTIVE: To evaluate the effect of mouse nerve growth factor injection via different ways on the treatment of peripheral nerve injury.
    METHODS: Totally 52 patients with peripheral nerve injury were randomly assigned into two groups: experimental group (local injection of mouse nerve growth factor, n=27) and control group (systemic administration of mouse nerve growth factor, n=25). The treatment was performed once a day, and lasted for 4 weeks. Then, the clinical efficacy and recovery of neurological function were compared.
    RESULTS AND CONCLUSION: The good and effective rates were 85% (n=23) and 93% (n=25) in the experimental group, while 72% (n=18) and 84% (n=21) in the control group, respectively, which were significantly better in the experimental group than the control group (P < 0.05). In the experimental group, 13 cases developed transient pain at injection site, including one case of remission undergoing oral analgesics; in the control group, 12 cases had transient pain at injection site, without any treatment. The results suggest that both local and total body injection of mouse nerve growth factor are safe and effective for treatment of peripheral nerve injury, but local injection is superior to systemic administration.



    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Tissue-engineered skin constructed by adipose-derived stem cell sheet
    Xiao Bin, Liu Yi
    2014, 18 (33):  5361-5365.  doi: 10.3969/j.issn.2095-4344.2014.33.020
    Abstract ( 289 )   PDF (580KB) ( 565 )   Save

    BACKGROUND: Wtih hyaluronic acid and collagen by cell autocrine as endogenous scaffolds, cell sheet technology can eliminate the disadvantages of exogenous scaffolds. Human adipose-derived stem cells combined with cell sheet technology used in the study of full-thickness skin defects have become the current hot spot.
    OBJECTIVE: To summarize the research progress in adipose-derived stem cell sheet.
    METHODS: Articles related to adipose-derived stem cells, cell sheet technology and tissue-engineered skin were retrieved in VIP information and PubMed database from January 1993 to April 2014. The key words were “adipose-derived stem cell, wound healing, tissue-engineered skin, cell sheet” in English and Chinese, respectively. Totally 27 relevant articles were analyzed.
    RESULTS AND CONCLUSION: Adipose-derived stem cells derived from discarded human adipose tissue are immmunocompatible and multi-potent, rendering them ideal for regenerative medicine applications. Cell sheet technology seemingly provides several advantages over traditional tissue reconstruction with biodegradeable scaffolds. Adipose-derived stem cells combined with cell sheet technology can enhance tissue vascularization, promote wound healing, the secreted extracellular matrix provides the microenvironment for the cell differentiation and growth, so it is expected to build a real three-dimensional tissue-engineered skin.



    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Role of Wnt signal pathway in osteoblasts: bone-forming or bone-resorbing? 
    Liu Yan-ling, Li Fang-bing, Zhao Xi
    2014, 18 (33):  5366-5371.  doi: 10.3969/j.issn.2095-4344.2014.33.021
    Abstract ( 1478 )   PDF (560KB) ( 1046 )   Save

    BACKGROUND: Wnt signal pathway is involved in the regulation of bone marrow mesenchymal stem cells differentiating into osteoblasts, promotes osteoblasts proliferation and differentiation, inhibits programmed death of osteoblasts, and indirectly affects the function of osteoclasts.
    OBJECTIVE: To summarize the relationship between Wnt/β-catenin signal pathway and bone disease.
    METHODS: A computer-based online search was performed to find papers published between January 2000 and January 2014 in CNKI database and Elsevier database. The key words were “Wnt/β-catenin, osteoblasts, bone marrow mesenchymal stem cells, osteoarthritis, chondrocytes” in Chinese. Documents concerning the effects of Wnt/β-catenin signal pathway on osteoblasts and bone disease were included.
    RESULTS AND CONCLUSION: Wnt signaling pathway is consisted of Wnt/β-catenin signal pathway (Wnt typical signal pathway), Wnt/Ca2+ signal pathway and Wnt/planar cell polarity signal pathway. Wnt signaling pathway is one of the most important regulatory systems that plays a key role in modulating the differentiation, proliferation and programmed cell death of osteoblasts, osteoclasts and chondrocytes. The role of Wnt signal pathway in patients with rheumatoid arthritis is mediated by osteoblasts. The inhibitory factor of Wnt signal pathway in osteoblasts is upregulated, which reduces the ratio of osteoprotegerin/receptor activating factor ligand, promotes the osteoclasts differentiation and immaturation. The researches addressing the components and effect of Wnt signal pathway are important for the special treatment of bone diseases and the prevention of osteoporosis or other bone diseases.



    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Mechanism of estrogen and estrogen-related compounds in osteoarthritis: joint protection and repair of bone cartilage cells
    Wang Jian, Tao Hai-rong
    2014, 18 (33):  5372-5376.  doi: 10.3969/j.issn.2095-4344.2014.33.022
    Abstract ( 385 )   PDF (570KB) ( 349 )   Save

    BACKGROUND: The bone metabolism of osteoarthritis is regulated by estrogen with osteoblasts, osteoclasts and cytokines, as well as a number of regulatory pathways.
    OBJECTIVE: To describe the role of estrogen and estrogen-related compounds for joint protection, repair of bone and cartilage cells, and inhibition of synovitis in osteoarthritis.
    METHODS: Author researched PubMed, Embase, Elseveir database from 1992 to 2014, with the key words of “osteoarthritis, estrogens, matrix metalloproteinases, interleukins, tumor necrosis factor-alpha”. After the quality of the included studies was evaluation, valid data were extracted and analyzed.
    RESULTS AND CONCLUSION: Estrogen can increase the expression of osteoprotegerin and nuclear factor-κB factor ligands in osteoblasts, inhibit bone resorption, prevent the onset and progression of osteoarthritis. Estrogen upregulates anti-osteoclast cytokines, downregulates pro-osteoclast factors, and contribute to regulate bone metabolism of osteoarthritis patients through bone morphogenetic protein and Wnt signaling. Estrogen promotes the adrenal cortex secretion of glucocorticoids and indirectly inhibits the production of matrix metalloproteinases by the hypothalamus-hypophysis-adrenal gland axis. Exogenous estrogen inhibits bone resorption, which may help to delay the development of osteoarthritis. Estrogen and estrogen-related compounds may inhibit the cartilage loss caused by synovitis and inflammatory factors in the late stage of osteoarthritis.



    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Acromioclavicular joint dislocation and reconstruction
    Wang Zhi-zhou, Yilihamu•Tuoheti
    2014, 18 (33):  5377-5383.  doi: 10.3969/j.issn.2095-4344.2014.33.023
    Abstract ( 474 )   PDF (685KB) ( 587 )   Save

    BACKGROUND: At present, Rockwood type I, II acute dislocation of acromioclavicular joint is recommended to be treated non-operatively, while acute dislocation of type IV, V and VI should be treated operatively. The treatment of type III acute dislocation of acromioclavicular joint remains controversial. Furthermore, there are a variety of methods to repair the dislocation of acromioclavicular joint. Among them, the anatomical reconstruction of coracoclavicular ligament and acromioclavicular ligament to restore the biomechanics of acromioclavicular joint are the developing trend. Therefore, more and more scholars pay attention to the anatomical structure of acromioclavicular joint.
    OBJECTIVE: To summarize the research status of the repair and reconstruction of acromioclavicular joint dislocation.
    METHODS: A computer-based retrieval was performed among Wanfang database (http://g.wanfangdata.com.cn/) and PubMed database (http://www.ncbi.nlm.nih.gov/PubMed) by the first author until May 2014, for articles about acromioclavicular joint structure and acromioclavicular joint dislocation. The key words were “acromioclavicular, acromioclavicular joint dislocation, endobutton” in English, and “acromioclavicular joint dislocation, anatomical reconstruction” in Chinese. After repeated studies were excluded, 1 632 articles were screened out, and 43 of them were involved in the analysis.
    RESULTS AND CONCLUSION: Surgical methods of acromioclavicular joint dislocation are various. Now the treatment trends to anatomical reconstruction, including tendon transplantion and endobutton technique. Based on it, more and more basic researches of the acromioclavicular joint emerge. But due to the lack of large sample, multi-center clinical control study, the optimal treatment of the acromioclavicular joint dislocation is at the exploration stage.



    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Reconstruction of anterior cruciate ligament repairs knee sports injury: clinical state and future 
    Zhang Hai-ling
    2014, 18 (33):  5384-5388.  doi: 10.3969/j.issn.2095-4344.2014.33.024
    Abstract ( 442 )   PDF (625KB) ( 603 )   Save

    BACKGROUND: The cruciate ligament of the knee joint has a poor capacity of self-healing after injury, and the main treatment is grafting to reconstruct cruciate ligament.
    OBJECTIVE: To summarize the structure, function, and mechanical characteristics of anterior cruciate ligament of the knee joint, the present research of artificial ligament reconstruction following damage, and to provide evidences for clinical application of artificial ligament.
    METHODS: An online computer-based retrieval was performed with the key words of “artificially synthetic materials, knee joint cruciate ligament, exercise, ligament repair, ligament reconstruction”. Articles related to knee ligament treatment approach, materials science characteristics, biocompatibility and application were included. A total of 25 articles addressing the properties of biological materials applied in knee cruciate ligament reconstruction were screened.
    RESULTS AND CONCLUSION: As an effective treatment for anterior cruciate ligament injuries, technique of anterior cruciate ligament reconstruction has been developed in recent years. Implants for repairing anterior cruciate ligament injuries include autologous ligaments, ligament allograft, artificial ligaments, and tissue engineered ligament. Artificial materials and tissue engineered ligaments are the hotspots in recent years. Biological artificial ligament is effective to treat the knee ligament injury. Cell and molecular biology techniques as well as the use of gene therapy will become a new direction in the treatment of knee ligament injury and rehabilitation.



    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Androgen responses to resistance exercise
    Wen An
    2014, 18 (33):  5389-5395.  doi: 10.3969/j.issn.2095-4344.2014.33.025
    Abstract ( 886 )   PDF (596KB) ( 586 )   Save

    BACKGROUND: The androgen response to resistance exercise is one of hot topics in sports science research. At present, the androgen responses to resistance exercise show different results. There are different androgen changing trends under different resistance exercise interventions.
    OBJECTIVE: To have a deep insight into the influence of resistance exercise on the response of human body androgen, clarify research progress in androgen response to resistance exercise, and provide the basic theory for monitoring resistance training.
    METHODS: Literatures and paper reports about androgen response to resistance exercise are electronically retrieved from the American Biological Medical Literature Database PubMed from 1986 and 2013. Finally 46 literatures addressing the research progress is analyzed about androgen responses to resistance exercise were included.
    RESULTS AND CONCLUSION: Studies have shown that, weight lifting (power snatch and power clean), squating, and deadlifting produced higher testosterone levels than the small muscle groups. Under the same load, eccentric and concentric contractions produced the same hormone response. Adequate training amount and intensity may change the circulating testosterone levels. Short sports intermittent time led to a higher testosterone response than the long intermittent time. The free testosterone levels in youth group were significantly increased compared with the older group before and after exercise. Nutrition intervention affected acute testosterone responses to resistance exercise. Changes in exercise intensity and amount will alter quiet testosterone levels, once returned to normal training, testosterone levels will return to baseline values. Resistance exercise can increase androgen receptor content in human skeletal muscles.



    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    A meta-analysis of ultrasonic therapy on relieving pain of knee osteoarthritis patients
    Yang Ye, Zeng Chao, Deng Zhen-han, Zhang Yi, Li Yu-sheng, Li Hui, Yang Tuo, Lei Guang-hua
    2014, 18 (33):  5396-5401.  doi: 10.3969/j.issn.2095-4344.2014.33.026
    Abstract ( 455 )   PDF (709KB) ( 931 )   Save

    BACKGROUND: Ultrasonic therapy is one of several physical therapy modalities suggested for the management of pain and loss of function due to osteoarthritis. However, its effectiveness still remains controversial in the previous studies.
    OBJECTIVE: To analyze the effect of ultrasonic therapy for the treatment of relieving knee osteoarthritis pain.
    METHODS: A retrieval of Pubmed, Ovid/Medline, Ovid/EMBASE, and Cochranee database was performed. The relevant literatures were manually retrieved. The retrieval deadline was set on March 31, 2014. Randomized controlled trials on ultrasonic therapy of knee osteoarthritis were collected.
    RESULTS AND CONCLUSION: A total of eight studies of meta-analysis were accumulated. Among them, six studies adopted visual analog scale and Western Ontario and McMaster Universities Arthritis Index, one study adopted visual analog scale only, and one study adopted Western Ontario and McMaster Universities Arthritis Index. Then the Western Ontario and McMaster Universities Arthritis Index scores were transformed into visual analog scale scores for data analysis. There was a statistical difference between the groups in the visual analog scale pain score (standardized standard deviation: -0.51; 95% confidence interval: -0.68, -0.33; P=0.05). Ultrasonic therapy is an effective method for knee osteoarthritis pain.



    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Correlation between acid-suppressive medications and risk of osteoporosis-related fractures: a meta-analysis
    Dong Rui, Xiang Zheng, Lin Han-sheng
    2014, 18 (33):  5402-5406.  doi: 10.3969/j.issn.2095-4344.2014.33.027
    Abstract ( 361 )   PDF (590KB) ( 369 )   Save

    BACKGROUND: Whether a long-term use of acid-suppressive medication for over 3 years can increase the risk of fractures, remains controversial.
    OBJECTIVE: To systematically review the effect of acid-suppressive medication on fracture risk.
    METHODS: Clinical trials about the relationship of acid-suppressive medication use and risk of osteoporosis-related fractures were searched in MEDLINE, Cochrane Library, EMbase, and CNKI database. We also manually searched some published and unpublished references. Study selection and assessment, data collection and analyses were undertaken by two reviewers independently. The Cochrane Collaboration’s RevMan 5.0 software was used for data analyses.
    RESULTS AND CONCLUSION: After a comprehensive search, 11 original clinical trials were included in this meta-analysis, including 3 nested case-control studies, 5 case-control studies, and 3 cohort studies. Patients with fractures were more likely than controls to have previously received supply of proton pump inhibitors [odds ratio (OR)=1.29, 95% confidence interval (CI) (1.18-1.41), P < 0.001]. The meta-analysis did not find a significant difference in the patients who received H2 receptor antagonists [OR=1.10, 95%CI (0.99-1.23), P=0.34]. A long-term use of proton pump inhibitors is associated with an increased risk of fracture in elderly persons already at a risk for osteoporosis.



    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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    Endoscopic radiofrequency ablation versus traditional open surgeries for treatment of gluteal muscle contracture: a meta analysis
    Ren Shi-you, Jiang Chang-qing, Li Wei, Zhang Wen-tao
    2014, 18 (33):  5407-5412.  doi: 10.3969/j.issn.2095-4344.2014.33.028
    Abstract ( 327 )   PDF (711KB) ( 705 )   Save

    BACKGROUND: A large number of studies have reported that endoscopic radiofrequency ablation surgeries were better than traditional open surgeries for gluteal muscle contracture, but there is no meta-analysis on the clinical outcomes of endoscopic surgeries versus traditional open surgeries.
    OBJECTIVE: To evaluate the effects of endoscopic radiofrequency ablation surgeries versus traditional open surgeries in the treatment of gluteal muscle contracture.
    METHODS: Eligible studies were identified from electronic databases including EMbase, Medline, PubMed, OVID, Cochrane Library, Springerlink, CNKI, WanFang, and VIP between January 1970 and May 2014. The literatures about the clinical efficacy of endoscopic radiofrequency ablation surgeries versus traditional open surgeries in the treatment of gluteal muscle contracture were retrieved. We screened the retrieved literature according to the inclusion and exclusion criteria and performed a Meta analysis with the software RevMan 5.2 after identification of the relevant data.
    RESULTS AND CONCLUSION: A total of 830 patients from 14 studies were included for the analysis, including 394 patients who underwent endoscopic surgeries and 436 patients who underwent traditional open surgeries. Among the main outcomes measured, the incidence of postoperative pain (relative risk=0.33, 95% confidence interval (CI): 0.27-0.42, P < 0.001) and postoperative complications (odds ratio=0.40, 95%CI: 0.23-0.70, P=0.001)  in the endoscopic surgery group were significantly lower than that in the traditional open surgery group. The curative effects showed no significant difference between the two groups (odds ratio=1.09, 95%CI: 0.52-2.26, P=0.82). Among the secondary outcomes measured, the incision length, postoperative off-bed activity time and postoperative hospitalization in the endoscopic surgery group were significantly better than that in the traditional open surgery group (P < 0.05). There was no significant difference in the surgery duration and recurrence rate (P > 0.05) between the two groups. Endoscopic radiofrequency ablation surgeries are similar to traditional open surgeries in the curative effects, recurrence rate and surgery duration, and are better than traditional open surgeries in the incisional length, postoperative pain, postoperative off-bed activity time, postoperative hospitalization and postoperative complication.



    中国组织工程研究
    杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


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