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20 August 2014, Volume 18 Issue 34 Previous Issue    Next Issue

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Imaging evaluation of hydroxyapatite/gel nano-composite in rabbit skull defect repair Geng Hai-xia, Guo Xiu-juan, Qian Jun-rong, Feng Wei 2014, 18 (34):  5413-5417.  doi: 10.3969/j.issn.2095-4344.2014.34.001 Abstract ( 405 )   PDF (616KB) ( 423 )   Save  BACKGROUND: Hydroxyapatite/gel nano-composite has the same mechanical strength to the natural bone, but its ability to repair bone defects and osteogenic effect need to be confirmed by further studies. OBJECTIVE: To explore the repair effect of hydroxyapatite/gel bionic composite in skull defects of rabbits. METHODS: The hole-like calvarium defect models were established in rabbits, and treated with hydroxyapatite/ gel composites (hydroxyapatite/gel group), autologous skull as positive control (autologous bone group) and nothing as negative control (blank group). The repairing condition in the skull defect areas were observed and analyzed by X-ray and CT at 4, 8, 12 weeks after implantation.  RESULTS AND CONCLUSION: After 8 weeks, X-ray assessment showed that normal-like bone tissue appeared in the defect region of the autologous bone group; in the hydroxyapatite/gel group, dense bone with similar morphology to normal bone tissue was found in the central site of defect region, and the boundary was slightly blurred. After 12 weeks, the hydroxyapatite/gel showed blurred edge compared with autologous bone, and the center of the composite was disconnected; in the blank group, a clear and regular transmitted shadow was observed. After 12 weeks, CT examination showed that the hydroxyapatite/gel was connected tightly with the surrounding normal bone tissue. As a new bionic composite, the hydroxyapatite/gel can achieve good effect in repairing skull defects of rabbits. 中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程 全文链接： Figures and Tables | References | Related Articles | Metrics

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Shaping ability of ProTaper Next and ProTaper Universal Ni-Ti rotary endodontic instruments in simulated root canals Liu Wen-zhe, Chen Guang-sheng 2014, 18 (34):  5418-5422.  doi: 10.3969/j.issn.2095-4344.2014.34.002 Abstract ( 1526 )   PDF (552KB) ( 1171 )   Save BACKGROUND: Currently, the nickel-titanium file has no uniform international standards for clinical use, and several nickel-titanium systems have unique designs in terms of cross-sectional shape, taper change, composition number, central steel volume, and cutting edge angle, so there are some differences in cleanup capability, security, and cutting efficiency. ProTaper Next is developed based on the ProTaper Universal, and its composition number, blade cross-sectional shape, contact point with the root canal wall and the tip tapers are all improved. OBJECTIVE: To compare the shaping ability between ProTaper Next and ProTaper Universal Ni-Ti rotary endodontic instruments by preparing the simulated root canals. METHODS: Two groups of resin blocks were prepared by ProTaper Next and ProTaper Universal respectively. Preparation time and incidence of canal aberration and instruments failure were recorded. After preparation, the images taken before and after preparation were superimposed and analyzed by software Adobe Photoshop v7.0. We measured the amount of resin removed at the inner and outer canal walls. The centering ability was also assessed. RESULTS AND CONCLUSION: There was no difference in the incidence of canal aberration. ProTaper Next was faster than ProTaper Universal to prepare canals (P < 0.05). Both instruments caused apical transportation. ProTaper Universal F2 removed more materials than ProTaper Next X2 at the inner canal walls (P < 0.05). ProTaper Next showed better centering ability than ProTaper Universal in most levels (P < 0.05). Two instruments showed good performance in preparation but both created some apical transportation. However, the ProTaper Next was better in maintaining the original form of curved canal with safety and higher efficiency. 中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程 全文链接： Figures and Tables | References | Related Articles | Metrics

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Biocompatibility of Genipin cross-linked type I collagen with human adipose-derived stem cells in vitro Wang Gang, Kang Ting, Liu Yi, Liu Gang-qiang 2014, 18 (34):  5423-5428.  doi: 10.3969/j.issn.2095-4344.2014.34.003 Abstract ( 389 )   PDF (2636KB) ( 884 )   Save BACKGROUND: Low toxicity of Genipin has certain species and cell specificity. Biocompatibility of Genipin cross-linked type I collagen with human adipose-derived stem cells is essential for construction of tissue-engineered adipose. OBJECTIVE: To investigate the bbiocompatibility of Genipin cross-linked type I collagen with human adipose-derived stem cells. METHODS: Human adipose-derived stem cells were isolated and cultured to the third generation, and the cells were seeded on Genipin cross-linked type I collagen scaffold. MTT assay was used to evaluate the adhesion and proliferation of cells on the scaffold, and the toxic effects of Genipin cross-linked type I collagen on human adipose-derived stem cells. Optical microscopy and scanning electron microscopy were utilized to observe the adhesion and growth process of human adipose-derived stem cells on the scaffold as well as the morphological changes of cells. RESULTS AND CONCLUSION: Human adipose-derived stem cells could adhere to the scaffold immediately after seeded and increase gradually on the scaffold, with the average adhesion rate of 86.5%. Optical microscopy and scanning electron microscopy showed that human adipose-derived stem cells adhered well on the scaffold. The cells increased gradually over time, and could migrate into the scaffold, and distribute evenly with the passage of time when observed with optical microscopy. The result showed Genipin possesses very low cytotoxicity to the cells, and the outstanding biocompatibility is found between the cells and scaffold in vitro after cross-linked with Genipin. 中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程 全文链接： Figures and Tables | References | Related Articles | Metrics

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Safety and histocompatibility of a novel biogradable stent implanted into the coronary artery in a porcine model Lu Zhao, Jiang Xue-jun, Feng Gao-ke, Zheng Xiao-xin, Li Jun, Qin Chao-shi, Gu Wei-wang, Wang Qun, Xu Qing-ru, Huang Yi-mei, Chen Jiu-hao 2014, 18 (34):  5429-5433.  doi: 10.3969/j.issn.2095-4344.2014.34.004 Abstract ( 400 )   PDF (2231KB) ( 441 )   Save BACKGROUND: In order to solve the problems of poly-l-lactic acid (PLLA) stents, such as poor support, acidic metabolites, we researched a novel biogradable stent-PLLA/amorphous calcium plosphate (ACP). OBJECTIVE: To discuss the safety and histocompatibility of the novel biogradable stent-PLLA/ACP stent implanted in the coronary artery in a porcine model. METHODS: Sixteen novel biogradable stents were randomly implanted into the coronary arteries, left anterior descending branch, left circumflex artery or right coronary artery of sixteen healthy Tibet miniature pigs. The blood routine and blood biochemistry were measured pre-operation and at 1 month after operation. The coronary blood vessels where the stent was implanted were examined by hematoxylin-eosin staining at 1 and 6 months after operation. RESULTS AND CONCLUSION: Compared with pre-operation, the post-operation indicators of the blood routine and blood biochemistry were of no significant difference. Coronary angiography revealed coronary artery patency and no thrombosis, the vascular stent segments exhibited clear boundaries with the surrounding tissue, with no tissue adhesion, necrosis, and adherence abnormalities. The results of hematoxylin-eosin staining showed that there was no significant difference in vascular injury integral between 1 month after operation and 6 months after operation. However, 6 months after operation, the scores of the inflammation were lower (P < 0.05), and the scores of the endothelialization were increased (P < 0.05). There was no myocardial infarction and inflammatory cell infiltration around the stent. These results suggest that the novel biodegradable stent has good safety and histocompatibility. 中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程 全文链接： Figures and Tables | References | Related Articles | Metrics

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Chondrogenesis of synovial mesenchymal stem cells co-cultured with chondrocytes on the three-dimensional scaffold Ning Xiao-ting, Shao Bo, Gong Zhong-cheng, Liu Hui, Ling Bin, Keremu Abass, Lin Zhao-quan, 2014, 18 (34):  5434-5440.  doi: 10.3969/j.issn.2095-4344.2014.34.005 Abstract ( 278 )   PDF (3128KB) ( 346 )   Save BACKGROUND: Articular chondrocytes with the ability of autocrine and paracrine can provide the growth factors and microenvironment for synovial mesenchymal stem cells differentiating into the chondrocyte. The three-dimensional scaffold could provide space for cells adhesion, proliferation and differentiation. OBJECTIVE: To study the ability of chondrogenesis by co-culturing synovial mesenchymal stem cells and chondrocytes under the three-dimensional condition. METHODS: The synovial membrane and articular cartilage were harvested from rat knee joint. The synovial mesenchymal stem cells and chondrocytes were obtained through the method of enzyme digestion. The passage 3 synovial mesenchymal stem cells and passage 2 chondrocytes were co-cultured in the chitosan/I collagen composite scaffolds at the ratio of 1:2. Then, the cells/scaffold composite was harvested to be examined morphologically, histologically and immunohistochemically after being cultured 21 days. The confocal laser was also employed to detect the cells distribution in the scaffold.  RESULTS AND CONCLUSION: After being cultured 72 hours, it could be observed from the cells/scaffold composite examined through the scanning electron microscope that the cells adhered on the surface of the scaffold and extracellular matrix surrounding the cells was seen on the scaffold. After being cultured 21 days, it could be found through the confocal laser scanning that the cells were well-distributed on the scaffold, and cells decreased gradually. Type II collagen was positive in the extracellular matrix immunohistochamically. It suggested from this study that the synovial mesenchymal stem cells could be co-cultured with chondrocytes in the chitosan/I collagen composite scaffolds and have the ability of chondrogenesis differentiation. 中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程 全文链接： Figures and Tables | References | Related Articles | Metrics

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Acellular dermal matrix allograft combined with coralline hydroxyapatite repair periapical tissue defects Xu Jun, Wang Jin-tao, Li Gang, Shi Fang-chuan, Zhong Liang-jun 2014, 18 (34):  5441-5448.  doi: 10.3969/j.issn.2095-4344.2014.34.006 Abstract ( 316 )   PDF (407KB) ( 468 )   Save BACKGROUND: Chronic periapical periodontitis often causes periapical tissue defects and ultimately leads to the loss of teeth if the inflammation is not promptly cleared to terminate bone resorption and destruction of gingival tissue. Acellular dermal matrix allograft and coralline hydroxyapatite are the common materials to repair periodontal injury. OBJECTIVE: To evaluate clinical efficacy of acellular dermal matrix allograft combined with coralline hydroxyapatite in repairing periapical tissue defects.  METHODS: A total of 76 patients of chronic apical periodontitis were randomly divided into two groups, with 38 cases in each group. In the experimental group, periapical tissue defects were treated with acellular dermal matrix allograft and coralline hydroxyapatite. In the control group, tissue defects were not treated. All the involved patients underwent apicectomy and retrograde filling. Clinical parameters and radiographic film were recorded at 1 week, 6 months and 3 years follow-up visits to evaluate the repairing effects.  RESULTS AND CONCLUSION: After 1 month of treatment, all acellular dermal matrix allografts survived, and the defect of gingival tissues that caused by repairing fistula had been healed. After 3 years, the repairing efficiency in the experimental group was significantly higher than that in the control group (P < 0.05). The bone defect disappeared in the experimental group at 6 months, the transmission of coralline hydroxyapatite particles was decreased, and there were some fuzzy images of compact density. This suggested that new bone was growing. The density of coralline hydroxyapatite particle got closed to normal bone tissue after 3 years, and there were transitional changes of density between coralline hydroxyapatite with normal bone. Coralline hydroxyapatite particle gradually fused with alveolar bone. Acellular dermal matrix allograft and coralline hydroxyapatite have good biological compatibility. In repairing periapical tissue defects, the application of acellular dermal matrix allograft combined with coralline hydroxyapatite is effective in clinical practice. 中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程 全文链接： Figures and Tables | References | Related Articles | Metrics

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Biodegradable and non-biodegradable drug-eluting stents and bare metal stents in acute myocardial infarction Yang Zhen 2014, 18 (34):  5449-5454.  doi: 10.3969/j.issn.2095-4344.2014.34.007 Abstract ( 482 )   PDF (597KB) ( 500 )   Save BACKGROUND: Large randomized controlled clinical trials have shown that drug-eluting stents has a lower restenosis rate compared with bare-metal stents, but the drug-eluting stents cannot reduce major cardiovascular events and death rates, and long-term follow-up of clinical registries show the drug-eluting stents even may increase late stent thrombosis events. OBJECTIVE: To evaluate the incidence of stenosis and adverse reactions after treatment with biodegradable, non-biodegradable drug-eluting stents and bare-metal stents in patients after myocardial infarction. METHODS: We retrospectively analyzed the clinical data of 236 cases of acute myocardial infarction, in which 79 cases were treated with biodegradable sirolimus-eluting stents, 83 cases treated with non-biodegradable sirolimus-eluting stents, and 74 cases treated with bare metal stents. Then, we compared late lumen loss and restenosis within 12 months after stent implantation and major adverse cardiac reactions within 24 months after stent implantation. RESULTS AND CONCLUSION: After 12 months, the late lumen loss of the bare stent group was significantly higher than that of biodegradable and non-biodegradable sirolimus-eluting stent groups (P < 0.05), but the difference in stent restenosis rate (P > 0.05). After 24 months, there were no differences in death, cardiac death, recurrent myocardial infarction, target vessel revascularization, target lesion revascularization, major adverse cardiac events and stent thrombosis events among the three groups. These three kinds of stents need to be further studied in their long-term efficacy and safety as percutaneous coronary intervention for acute ST-segment elevation myocardial infarction. 中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程 全文链接： Figures and Tables | References | Related Articles | Metrics

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Preparation and characterization of ciliary neurotrophic factor sustained-release microcapsules Li Xiao-li, Wang Ming-bo, Chen Chang-sheng, She Zhen-ding 2014, 18 (34):  5455-5460.  doi: 10.3969/j.issn.2095-4344.2014.34.008 Abstract ( 369 )   PDF (2255KB) ( 394 )   Save BACKGROUND: Conventional ophthalmic delivery of ciliary neurotrophic factor (CNTF) is extremely difficult to pass the blood-retinal barrier, resulting in a very low bioavailability and the need of long-term drug delivery. To solve the problem, the CNTF can be encapsulated in a semi-permeable membrane to form a microcapsule, which may then achieve the release of bioactive substances encapsulated, or bioactive molecules secreted by living cells and small molecular metabolites through semi-permeability of the special membrane. OBJECTIVE: To prepare a special structural CNTF sustained-release microcapsule. METHODS: A selected poly(ether sulfone) hollow fiber was cut into 1 cm long with its two ends sealed by 1181-M medical adhesive using UV curing. To prepare CNTF encapsulated microcapsule, one end was first sealed, and then the CNTF was loaded to poly(ether sulfone) microcapsule from the other end which then was sealed. The leaching liquor of sustained-release microcapsule was co-cultured with mouse fibroblast L929, to observe the cytotoxicity of the microcapsule. The sustained-release microcapsule was co-cultured with mouse retinal pigment epithelial cells, to observe the cell adhesion ability of the microcapsule. The CNTF sustained-release microcapsule was immersed in physiological saline, to observe the degradability. Moreover, in vitro release behavior of immunoglobulin and CNTF were evaluated. RESULTS AND CONCLSION: The CNTF sustained-release microcapsule had an inner diameter of about 398 μm and a membrane thickness of about 145 μm. The microcapsule presents a lot of macropores in the outer wall and many 10 nanometers micropores in the inner wall. The sustained-release microcapsule was not degraded in saline within 4 months, indicating good cell compatibility. The microcapsule can selectively release CNTF while protecting against invading of antibodies (IgG), showing its good selective permeability. Meanwhile, the sustained-release microcapsule improved the initial burst release of traditional drug delivery vesicles. The microcapsule presents a mild sudden release in the middle stage, and then a sustained release. 中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程 全文链接： Figures and Tables | References | Related Articles | Metrics

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Hemostasis and in vivo degradation of thermosensitive chitosan hemostatic film Yin Gang, Chen Hui-hao, Guo Xing-feng, Wei Chang-zheng, Hou Chun-lin 2014, 18 (34):  5461-5465.  doi: 10.3969/j.issn.2095-4344.2014.34.009 Abstract ( 492 )   PDF (2805KB) ( 611 )   Save BACKGROUND: The thermosensitive chitosan is a kind of chitosan, its hemostatic effect, tissue compatibility and in vivo absorption need further investigations. OBJECTIVE: To investigate the hemostasis, in vivo degradation and tissue compatibility of thermosensitive chitosan hemostatic film. METHODS: A total of 48 Sprague-Dawley rats were randomly divided into four groups, and carried out two experiments at the same time. (1) The incisions of the liver in three groups were covered with the thermosensitive chitosan hemostatic film, cellulose hemostatic cotton and gelatin sponge, respectively. Blank control group received no treatment. The bleeding time and bleeding amount were recorded. (2) The incisions of the quadriceps femoris muscle of rats in the above three groups were embedded with the same hemostatic materials respectively. Blank control group was not embedded. At 1, 2, 3, 4, 6 weeks, the incision tissues of the liver and the quadriceps femoris muscle were harvested for observation. After 4 weeks, the incisions were observed with hematoxylin- eosin staining and transmission electron microscopy. RESULTS AND CONCLUSION: The bleeding time and bleeding amount of thermosensitive chitosan hemostatic film and cellulose hemostatic cotton groups were significantly lower than those of gelatin sponge and blank control groups (P < 0.05). After 6 weeks, the thermosensitive chitosan hemostatic film was absorbed completely. After 3 weeks, the cellulose hemostatic cotton was absorbed completely. After 2 weeks, the gelatin sponge was absorbed completely. The liver lobules of thermosensitive chitosan hemostatic film were complete, the liver cell were normal structure, showing light swelling and little inflammatory cell infiltration. Under transmission electron microscopy, the liver cells had integral structure, cell nucleus and organelles remained intact. The muscle fibers showed complete structure and little inflammatory cell infiltration. Under transmission electron microscopy, the muscle fibers ranked tidily, with integral cell nucleus and organelles. The thermosensitive chitosan hemostatic film has good hemostasis effect and tissue compatibility. 中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程 全文链接： Figures and Tables | References | Related Articles | Metrics

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In vitro cytotoxicity of the hydroxyapatite/barium titanate biological piezoelectric ceramic coating Kang Wen, Xu Guo-qiang, Diilnur Aji, Wei Qng, Ma Hui, Li Zhao-peng, Song Qian, Rufiya Zulati, Alina Abdujilil, Pahirdin Kaisar 2014, 18 (34):  5466-5472.  doi: 10.3969/j.issn.2095-4344.2014.34.010 Abstract ( 528 )   PDF (3012KB) ( 679 )   Save BACKGROUND: In order to optimize the biological activity of hydroxyapatite, previous experiments have used plasma spraying technique to prepare a piezoelectric ceramic coating on the surface of hydroxyapatite, but the cytotoxicity of this new material is not clear. OBJECTIVE: To evaluate the cytotoxicity of hydroxyapatite/barium titanate biological piezoelectric ceramic coating in vitro. METHODS: The 3rd generation beagle bone marrow mesenchymal stem cells were seeded on hydroxyapatite/barium titanate piezoelectric ceramic specimens and hydroxyapatite specimens, respectively. After 5 days, the cell adhesion was detected by scanning electron microscopy. The 3rd generation bone marrow mesenchymal stem cells were also co-cultured with hydroxyapatite/barium titanate piezoelectric ceramic specimen extract, hydroxyapatite specimen extract, low-glucose Dulbecco’s modified Eagle’s medium containing 5% dimethylsulfoxide and 15% fetal bovine serum, and low-glucose Dulbecco’s modified Eagle’s medium containing 15% fetal bovine serum, respectively. The cytotoxicity was tested by Cell Counting Kit-8 assay at days 1, 3, 5 after co-culture. RESULTS AND CONCLUSION: Bone marrow mesenchymal stem cells on the surface of hydroxyapatite/barium titanate piezoelectric ceramic specimens and hydroxyapatite specimens grew proliferatively and presented with multi-layer growth. The connection between cells and pseudopodia was very close, which indicates that the two kinds of materials both have good cytocompatibility. Cell Counting Kit-8 assay showed that the cells cultured in the extracts of hydroxyapatite/barium titanate biological piezoelectric ceramic and hydroxyapatite specimens proliferated more than 80%, and the toxicity was grade 1 that meant no cytotoxicity. 中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程 全文链接： Figures and Tables | References | Related Articles | Metrics

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Biomechanical evaluation and optimal design of two parameters of dental implant with arbitrarily adjusted angles    Cheng Si-yuan, Wen Hai-lin, Si Jing-qiu, Liang Rui, Nie Jing, Wang Hang, Long Jie, Tang Wei, Wei Yong-tao, Tian Wei-dong 2014, 18 (34):  5473-5479.  doi: 10.3969/j.issn.2095-4344.2014.34.011 Abstract ( 427 )   PDF (849KB) ( 710 )   Save BACKGROUND: Oversize stress of a dental implant and its surrounding tissue is the main factor to affect the long-term use of dental implants. So, the reasonable and precise design of implant shape is one of the important methods of prolonging the life span of dental implants. OBJECTIVE: To make the optimal analysis and design of the diameters of connector screw and central screw of the adjustable-angle dental implant invented in the earlier stage. METHODS: The finite element analysis model of the edentulous mandible with adjustable-angle dental implant was established by software Pro/E 5.0, Mimics 10.0 and ANSYS Workbench 14.5. The maximum equivalent stress of dental implant-edentulous mandibular model was analyzed. RESULTS AND CONCLUSION: The maximum equivalent stress of dental implant-edentulous mandibular model was much more sensitive to the diameter of connector screw than central screw. The maximum equivalent stress was the least when the diameters of connector screw and central screw were 1.29 and 0.95 mm, respectively, under vertical load as well as when the diameters of connector screw and central screw were 1.45 and 1.09 mm under lateral lingual load, respectively. The results of this research showed that the maximum equivalent stress in the madibular-dental implant-abutment model was more affected by the diameter of connector screw than that of central screw, indicating that more attention should be paid to the choice and design of connector screw. 中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程 全文链接： Figures and Tables | References | Related Articles | Metrics

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Development of a new open “C”-shaped molar band with nanomaterial Zhou Shan, Lu Xiao-li, Yang Li-jun, Li Yao, Zhang Yu, Zhang Xiao-peng 2014, 18 (34):  5480-5484.  doi: 10.3969/j.issn.2095-4344.2014.34.012 Abstract ( 507 )   PDF (575KB) ( 401 )   Save BACKGROUND: In the field of orthodontics, molar bands play an important role. But traditional bands have some weakness difficult to be solved in clinical use, for example, trying on cumbersomely, having to separate the adjacent teeth beforehand, occupying bigger space and tending to cause periodontitis of molars with bands. So some clinicians use a buccal tube instead of the molar band, but it has relatively high losing rate and cannot be fixed with Transpalatal Arch and Nance. OBJECTIVE: To invent a new open “C”-shaped molar band with nanomaterial (patent number: ZL201110057699.1) to solve the above problems as a common molar appliance.  METHODS: We made some stainless steel strips of 0.18-0.20 mm thickness and a certain length. The stainless steel strips were made to the new bands composed of buccal, lingal, distal surface and bilateral extention caused by removable parts extending to mesial surface. In order to improve retention force, its inner surface was coated by nano-hydroxyapatite and it bound to the molar crown by resin reinforced glass ionomer cements in clinical use. RESULTS AND CONCLUSION: The new open “C”-shaped molar band with nanomaterial overcomes shortcomings of conventional band and buccal tube. From the design, in contrast with traditional band, it not only has no need to separate teeth beforehand and to try on repeatedly, but also less stimulates periodontal tissues. So the new band can be more suitable for clinical use. 中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程 全文链接： Figures and Tables | References | Related Articles | Metrics

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Three kinds of periodontal stabilization splints in severe periodontitis of the mandibular anterior teeth: which one is preferred? Gao Xiao-wei, Chen Chong, Gulibaha Maimaitili 2014, 18 (34):  5485-5489.  doi: 10.3969/j.issn.2095-4344.2014.34.013 Abstract ( 381 )   PDF (667KB) ( 681 )   Save BACKGROUND: It is believed that the splintage can promote periodontal tissue repair and improve the prognosis of loose teeth. However, the force of the splint is associated with the rigidity of the splint and adaptation between the teeth and the splint. OBJECTIVE: To evaluate the therapeutic effects of the nylon composite resin periodontal splint, the fiber-reinforced composite periodontal splint and the bracket periodontal splint on the treatment of severe periodontitis of the mandibular anterior teeth. METHODS: Seventy-two patients with severe periodontitis of the mandibular anterior teeth were randomized into 3 groups, with 24 cases in each group: nylon composite resin periodontal splint, fiber-reinforced composite periodontal splint and bracket periodontal splint groups. The clinical effects of the three kinds of periodontal splints and periodontal indexes were compared at 1, 6 and 12 months after splinting. RESULTS AND CONCLUSION: The clinical effects in the group of the fiber-reinforced composite periodontal splint were significantly better than those in the other two groups at 1, 6, 12 months, which were 100%, 100% and 95.8%, respectively. The plaque index, bleeding index, and pocket depth were worse in the group of the nylon composite resin periodontal splint than the other two groups (P < 0.05). Results suggest that the fiber-reinforced composite periodontal splint is preferred to preserve the loosened teeth with severe periodontitis． 中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程 全文链接： Figures and Tables | References | Related Articles | Metrics

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Cloning and expression of Asperguillus endo-chitosanase gene in Escherichia coli Lu Hua-ding, Lian Li-yi, Chen Ming-wei, Dai Yu-hu 2014, 18 (34):  5490-5496.  doi: 10.3969/j.issn.2095-4344.2014.34.014 Abstract ( 412 )   PDF (877KB) ( 374 )   Save BACKGROUND: Chitosanase is an enzyme for efficient and special hydrolysis of chitoan, and hence its effective and stable expression with enzymatic activity will contribute to improving gene therapeutic effect. OBJECTIVE: To construct a chitosanase gene for the efficient and specifical hydrolysis of chitosan, and to investigate its expression in Escherichia coli and the main influencing factors of enzymatic activity. METHODS: According to the sequences of endo-chitosanase of Aspergillus sp. CJ22-326 provided in Genbank (EU302818), primers were designed and synthesized. The Asperguillus endo-chitosanase gene was amplified by successive extension PCR. And then the recombinant pET28a-His6-CSN was constructed and expressed in Escherichia coli BL21. Finally the recombinant His6-CSN fusion protein was analyzed by sodium dodecyl sulfate polyacrylamide gelelectrophoresis (SDS-PAGE), the western blot and dinitrosalicylic acid assay for detecting the enzyme activity of eluted His6-CSN fusion protein. The influence of different pH value and temperature on the enzyme activity of the recombinant chitosanase was investigated. RESULTS AND CONCLUSION: SDS-PAGE showed that 29 kDa proteins were expressed and the western blot assay showed that His6-CSN expressed successfully in the host. Dinitrosalicylic acid assay determined the enzymatic activity of His6-CSN was significantly higher than that of lysozyme, but lower than that of chitosanase from Streptomyces griseus (P < 0.05). The recombinant chitosanase displayed the maximal activity at temperature of 50 ℃ and pH value of 6.0. There were a higher enzymatic activity remaining at pH value of 4.0-7.0 and temperature of 30-50 ℃. 中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程 全文链接： Figures and Tables | References | Related Articles | Metrics

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Allogenic acellular extracellular matrix repairs high anal fistula   Wang Jian-cheng, Wang Wei, Zhang Ke, Zou Shi-zhen 2014, 18 (34):  5497-5502.  doi: 10.3969/j.issn.2095-4344.2014.34.015 Abstract ( 740 )   PDF (672KB) ( 2836 )   Save BACKGROUND: Treatment methods for high anal fistula include fistula removal, incision and thread drawing, selective mucosal flap displacement, fibrin glue closure, fistula liagtion between sphincter, most of which show many advantages, including long-term healing, a low success rate, high recurrence rate, and high postoperative complication rate. OBJECTIVE: To observe the clinical effect of acellular extracellular matrix in the treatment of high anal fistula, and to explore a minimally invasive treatment for high anal fistula. METHODS: Totally 100 cases of high anal fistula were randomly divided into treatment group and control group, 50 cases in each group. Treatment group were treated with allogenic acellular extracellular matrix, and control group were treated with traditional low incision with high thread-drawing. Then, we observed and compared the operation time, bleeding volume, postoperative pain score (visual analog scale score), postoperative pain duration, anal incontinence severity score (Wexner score), wound healing time, one-stage success rate, cure rate, recurrence rate. RESULTS AND CONCLUSION: Compared with the control group, the treatment group showed lower scores in the operation time, bleeding volume, wound healing time, visual analog scale score, postoperative pain duration, and anal incontinence severity score (P < 0.05), but higher scores in one-stage success rate and cure rate (P < 0.05). There was no difference in the recurrence rate between the two groups. These findings indicate that the allogenic acellular extracellular matrix for treatment of high anal fistula exhibits small trauma, quick recovery, short course of treatment, high cure rate and has no damage to the anal function and appearance. 中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程 全文链接： Figures and Tables | References | Related Articles | Metrics

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Performance evaluation of a cerebrovascular stent coated with bone marrow mesenchymal stem cells Tang Ying, Liu Li-na, Wang Qing-min, Liu Chang, Yang Yi-li 2014, 18 (34):  5503-5508.  doi: 10.3969/j.issn.2095-4344.2014.34.016 Abstract ( 296 )   PDF (2558KB) ( 370 )   Save BACKGROUND: Generally, the stent surface modification, especially seeding cells, may accelerate or cause stent endothelium, and cause restenosis for prevention of in-stent thrombosis. OBJECTIVE: To develop the optimal conditions for vascular stents coated with bone marrow mesenchymal stem cells. METHODS: Cerebrovascular stent was co-cultured with passage 3 bone marrow mesenchymal stem cells from rats at 1×106, 1×107, 1×108, and 1×109/L. Cells on the stents were examined with transmission electron microscopy after 48 hours. A total of 160 male Sprague-Dawley rats were enrolled, among which, 20 rats were as normal control group, and the remaining 140 were used for producing models of ischemic stroke that were randomly sub-divided into seven groups at 8 weeks after modeling: stainless steel stent implanted group, polymer stent group, and different concentrations of cell stent composite groups. After 8 weeks of implantation, the expression of vascular endothelial growth factor in these cells was examined by western blot assay. Rat platelet activation in different groups was determined by flow cytometry. RESULTS AND CONCLUSION: Implanted stem cells were able to grow adherently on the stainless steel stent wall. When the planting cell concentration was 1×107 cells/L, the cells and organelles were morphologically normal and covered the stent surface well. These coated cells also expressed vascular endothelial growth factor, suggesting that they functioned as endothelial cells, and they also significantly lowered platelet activation. When co-cultured with 1×107/L bone marrow mesenchymal stem cells, the stent was covered well with endothelial-like cells and had significant lower platelet activation in vivo. 中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程 全文链接： Figures and Tables | References | Related Articles | Metrics

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Phosphorylcholine coating enhances biocompatibility of expanded polytetrafluoroethylene used in polymeric prosthetic heart valves Zhang Ben, Gong De-jun, Zhang Xi-wu, Xu Tong-yi, Han Lin, Tang Hao, Lu Fang-lin, Xu Zhi-yun 2014, 18 (34):  5509-5514.  doi: 10.3969/j.issn.2095-4344.2014.34.017 Abstract ( 428 )   PDF (2130KB) ( 469 )   Save BACKGROUND: Our preliminary study found that the monocusp valves made of ultramicropore expanded polytetrafluoroethylene (ePTFE) revealed no significant thrombus, calcification, or degradation 20 weeks after implanted into the descending aorta and the left pulmonary artery in sheep, which verified the good property of ePTFE. However, the surface of ePTFE in the left pulmonary artery was covered with obvious neointima. OBJECTIVE: To assess the biocompatibility of phosphorylcholine-coated ePTFE. METHODS: ePTFE surface was modified by phosphorylcholine derivative. Then the changes of surface shape, tensile stress at yield and elasticity modulus, water contact angle, and protein absorption capacity of ePTFE after surface modification were observed. (1) Hemolytic test: the leaching solution of phosphorylcholine-coated ePTFE, leaching solution of uncoated ePTFE, normal saline, and distilled water were added to the diluted human blood, respectively. (2) Platelet count test: the phosphorylcholine-coated ePTFE, uncoated ePTFE, high density polyethylene, and Zymosan A were added to the whole blood samples from healthy volunteers, respectively. (3) Platelet activation test: the phosphorylcholine-coated ePTFE, uncoated ePTFE, γ-Globulins, and Zymosan A were added to the whole blood samples from healthy volunteers, respectively. RESULTS AND CONCLUSION: The mean micropore diameter of ePTFE was significantly decreased after phosphorylcholine coating (P < 0.001). The hydrophilicity and the ability of suppressing protein adsorption were significantly strengthened after phosphorylcholine coating (P < 0.001). Phosphorylcholine coating did not influence ePTFE in biomechanical properties and hemolytic test. The platelet count test and platelet activation test demonstrated that phosphorylcholine coating significantly improved anti-thrombus function of ePTFE. So, phosphorylcholine coating can enhance anti-thrombus function, suppress protein adsorption, and improve biocompatibility of ePTFE. 中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程 全文链接： Figures and Tables | References | Related Articles | Metrics

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Biological properties, degradation and absorption of collagen sponges in vivo Chi Yan-yan, Le Yao-jin, Liu Xu-zhao, Li Qi, Lei Jing, Tang Shun-qing 2014, 18 (34):  5515-5519.  doi: 10.3969/j.issn.2095-4344.2014.34.018 Abstract ( 1661 )   PDF (2108KB) ( 1404 )   Save BACKGROUND: Collagen sponges are applied for hemostatic use, wound healing, and residual cavity filling, which have great values in clinical application and scientific research. OBJECTIVE: To investigate the biological properties, biocompatibility and biodegradability of collagen sponges in vivo. METHODS: The spatial structure, pore diameter and porosity of collagen sponges were characterized by scanning electron microscopy. Transmission electron microscopy was used to observe the conformation of collagen sponges. The secondary structure and thermal denaturation temperature of collagen sponges were analyzed by circular dichroism spectrum. Collagen sponges were implanted intramuscularly into the spinal cord of New Zealand rabbits to observe the degradation and absorption and histological changes in vivo. RESULTS AND CONCLUSION: Collagen sponges had porous structure with varying pore sizes ranging  40-150 μm, the mean pore size of 100 μm, the thickness wall of 1 μm, and a porosity of approximately 95.8%. Collagen sponges had a typical porous structure and periodic light and dark zones. The solution of collagen sponges had a weak positive band near 220 nm and an intense negative band near 206 nm, which indicated a classic triple helix. And the secondary structure and thermal stability of collagen sponges were similar to that of liquid collagen. Collagen sponges began to degrade at 4 weeks, and remained 20% at 12 weeks. These sponges had been associated with foreign body response and inflammation within 2 weeks after implantation. With wound healing, inflammatory reactions gradually reduced and disappeared. During the implantation and degradation of sponges, no significant fibrous capsule formed and no tissue necrosis occurred at implantation site, indicating that collagen sponges have good performance in bioactivity, biocompatibility and degradation. 中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程 全文链接： Figures and Tables | References | Related Articles | Metrics

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The reproductive toxicity of rat models implanted with copper-bearing intrauterine device at teratogenic sensitive period by continuous extraction method Zhang Dan-dan, Wang Yi, Wang Chun-ren, Wang Zhao-xu 2014, 18 (34):  5520-5524.  doi: 10.3969/j.issn.2095-4344.2014.34.019 Abstract ( 376 )   PDF (701KB) ( 412 )   Save BACKGROUND: At present, the copper-bearing intrauterine device, a kind of class III medical devices, is commonly used in China. However, there is no clear conclusion about whether it has impact on the embryo or fetus in some cases, such as unexpected pregnancy during long-term implantation and pregnancy in a short time after removing it. OBJECTIVE: To evaluate the safety of copper-bearing intrauterine device by observing the influence of copper-bearing intrauterine device extracts on pregnant rats and rat fetuses by tail vein injection in the sensitive period of teratogenesis. METHODS: A total of 60 fertilized rats were divided into control group, high dosage group, middle dosage group, and low dosage group. The copper-bearing intrauterine device extracts were prepared by the continuous extraction method. Different concentrations (0.2, 0.1, 0.05 g/mL) of copper-bearing intrauterine device extracts were injected by the tail vein at the 1st day of pregnancy in the latter three groups at a dosage of 0.01 mL/g per day. The control group was given the same amount of normal saline. The injection lasted for 20 days. Then, the pregnant rats were sacrificed to measure body mass, check both sides of the uterus and internal organs, isolate fetal rats, as well as record the quality of uterus and fetal rats, corpus luteum, implantation numbers, the number of stillbirths, then number of live births and the number of fetal absorption. The fetal rats were determined in the following aspects: body mass, body height, tail length, the ossification degree and appearance of the occipital bone, bone and visceral anomalies. RESULTS AND CONCLUSION: The number of births, implantation numbers, the number of live births, the number of corpus luteum, the percentages of live births and stillbirths, the number of resorbed fetuses, and the weight of uterus and fetal rats in the control group showed no difference from those in the other three groups (P > 0.05). No malformation in the internal organs occurred. Compared with the control group, the high, middle and low dosage groups showed no difference in the height, tail length, body mass, and ossification degree of the occipital bone of fetal rats (P > 0.05). No malformation in the appearance, skeleton and internal organs occurred in the fetal rats. These findings indicate that there were no maternal toxicity, abnormal embryonic growth or rat fetus anomalies after injecting copper-bearing intrauterine device extracts into pregnant rats in sensitive period of teratogenesis. 中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程 全文链接： Figures and Tables | References | Related Articles | Metrics

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Minocycline hydrochloride slow-release antibacterial ointment in the treatment of combined periodontal-endodontic lesions Wang Miao-yan, Chen Guang-sheng, Zhuang Hai-yan 2014, 18 (34):  5525-5529.  doi: 10.3969/j.issn.2095-4344.2014.34.020 Abstract ( 371 )   PDF (514KB) ( 464 )   Save BACKGROUND: The role of minocycline hydrochloride in adjuvant therapy of chronic periodontitis and periodontal abscess has been widely reported, but detailed study of minocycline hydrochloride for combined periodontal-endodontic lesions is rare at present. OBJECTIVE: To investigate the effect of minocycline hydrochloride in the treatment of combined periodontal-endodontic lesions. METHODS: A total of 68 teeth with combined periodontal-endodontic lesions were treated with periodontal mechanical treatment and root canal therapy. According to the patient’s willing, 68 patients were divided into experimental group (n=24; local injection of minocycline hydrochloride after periodontal mechanical treatment), positive control group (n=24; local injection of iodine glycerol after periodontal mechanical treatment), and negative control group (n=20; no injection after periodontal mechanical treatment). These three groups were reviewed 1 month after periodontal mechanical treatment, and the differences before and after treatment were compared, including periodontal probing depth, attachment loss and gingival bleeding index. RESULTS AND CONCLUSION: Probing depth, attachment loss and gingival bleeding index in three groups were significantly improved after 1 month of periodontal mechanical treatment (P < 0.05); both probing depth and sulcus bleeding index in experimental group and positive control group were better than that of the negative control group (P < 0.05), and probing depth and sulcus bleeding index in experimental group were better than that of positive control group (P < 0.05). Experimental findings indicate that, in the clinical treatment of combined periodontal-endodontic lesions, in addition to periodontal mechanical treatment and root canal therapy, local application of minocycline hydrochloride can effectively reduce the depth of periodontal pocket, improve gingival inflammatory conditions and consolidate curative effect. 中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程 全文链接： Figures and Tables | References | Related Articles | Metrics

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Gelatin sponge impregnated with hemocoagulase reduces postoperative blood loss of patients with lumbar fractures undergoing posterior spinal decompression Du Jun-sheng, Wang Guang-yong, Zhong Bing 2014, 18 (34):  5530-5534.  doi: 10.3969/j.issn.2095-4344.2014.34.021 Abstract ( 366 )   PDF (661KB) ( 445 )   Save BACKGROUND: A single hemostatic material has been proved not to facilitate wound healing or to produce certain adverse reactions; while composites composed of two or three different materials can improve the advantage and histocompatibility of hemostatic materials. OBJECTIVE: To investigate the effect of gelatin sponge impregnated with hemocoagulase solution on amount of bleeding in patients with lumbar fractures undergoing posterior spinal decompression. METHODS: Fifty patients with lumbar fractures who were scheduled for open reduction, pedicle screw fixation and laminectomy were enrolled, including 25 cases treated with gelatin sponge impregnated with hemocoagulase before surgical incision closure as test group and 25 cases treated with single gelatin sponge before surgical incision closure as control group. Postoperative drainage volume, drainage time, length of stay, number of re-admissions and postoperative complications were compared between the two groups. RESULTS AND CONCLUSION: The postoperative drainage volume, drainage time and length of stay in the test group were significantly less than those in the control group (P < 0.000 1). No infection, epidural hematoma, or re-admission of patients was found, and there was no hemocoagulase-impregnated absorbable gelatin sponge- related adverse reaction. These findings indicate that posterior laminectomy with hemocoagulase-impregnated gelatin sponge can significantly reduce patients’ postoperative wound drainage and shorten the length of stay. 中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程 全文链接： Figures and Tables | References | Related Articles | Metrics

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Prolene hernia system versus polypropylene mesh plug in tension-free hernia repair Fan Zhong-bao, Rong Da-qing, Liu Qing-feng 2014, 18 (34):  5535-5539.  doi: 10.3969/j.issn.2095-4344.2014.34.022 Abstract ( 668 )   PDF (573KB) ( 557 )   Save BACKGROUND: Patch for tension-free inguinal hernia repair has been generally accepted by the majority of surgeons, but it is difficult for surgeons to select the proper patch type. OBJECTIVE: To compare the clinical efficacy of prolene hernia system versus polypropylene mesh plug in tension-free hernia repair. METHODS: A retrospective analysis was conducted in 150 patients with inguinal hernia, including 133 males and 17 females, aged 21-80 years old. Among them, 81 patients were operated with prolene hernia system, and 69 patients were subjected to polypropylene mesh plug. It was the first surgery for all patients. We concluded indications, and recorded operation time, out of bed time, the average hospitalization time, postoperative pain, foreign body sensation and discomfort situation, as well as relapse during the 2-year follow-up. RESULTS AND CONCLUSION: The prolene hernia system patch was suitable for large-bore hernial annulus, the weak transversalis fascia, inguinal canal with posterior wall defect. There were no significant differences between the two groups in operation time, out of bed time and the average hospitalization time, but the postoperative pain, foreign body sensation, discomfort situation and recurrence within 2-year follow-up were significantly lower in patients undergoing prolene hernia system than polypropylene mesh plug (P < 0.05). The results show that the prolene hernia system is a more ideal choice than the polypropylene mesh plug because of its wide indication, good tissue compatibility, slight local discomfort and foreign body sensation, low incidence of pain, and low relapse rate. 中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程 全文链接： Figures and Tables | References | Related Articles | Metrics

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Degradable natural polymer hydrogels in articular cartilage repair: safety and effectiveness assessment  Zhao Wen, Liu Yu-ying, Liu Zi-hao, Wang Mei 2014, 18 (34):  5540-5547.  doi: 10.3969/j.issn.2095-4344.2014.34.023 Abstract ( 442 )   PDF (632KB) ( 762 )   Save BACKGROUND: Polymer hydrogels composed of highly hydrated crosslinked network mimic the composites of the cartilage extracellular matrix and are embedded with articular chondrocytes not only to support chondrocytes growth but also to promote chondrocytes proliferation and division which will induce the cartilage regeneration and repair. OBJECTIVE: To review several kinds of natural polymer hydrogels and their newest progress and achievements. METHODS: Relative literatures from January 1994 to July 2013 were classified and analyzed by searching the databases through PubMed, Springer, Sciencedirect and Ovid databases using the keywords of “natural polymers, biodegradable polymers, hydrogel scaffold, articular cartilage, regeneration”. RESULTS AND CONCLUSION: Natural proteins (collagen, gelatin) and polysaccharides (chitosan, hyaluronic acid) are widely used as natural polymer hydrogels. The natural polymer hydrogels have not only the physical characters beneficial to articular cartilage regeneration, but also the important biological parameters, such as biocompatibility, low immunological response, low cytotoxicity and degradation. Meanwhile, the polymer hydrogels with natural origin can promote cell adhesion, proliferation and division, and enhance the regeneration of new tissue. Moreover, they also can serve as carriers to sustain the release of drugs and growth factors. All in all, natural polymer hydrogels have a potential application in articular cartilage regeneration and repair. 中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程 全文链接： Figures and Tables | References | Related Articles | Metrics

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Vacuum sealing drainage technique versus traditional repair in treatment of diabetic foot Zhu Xin-hua, Chai Yi-min, Ye Ji-zhong, Han Pei, Wen Gen, Chen Pei 2014, 18 (34):  5548-5554.  doi: 10.3969/j.issn.2095-4344.2014.34.024 Abstract ( 481 )   PDF (904KB) ( 623 )   Save BACKGROUND: Studies have shown that the vacuum sealing drainage technology can effectively promote the wound healing, and it has a wide prospect of clinical application, but there are few reports addressing the treatment of diabetic foot. OBJECTIVE: To discuss the clinical effect of vacuum sealing drainage technology in the treatment of diabetic foot wounds. METHODS: Sixty diabetic foot patients were randomly divided into two groups: traditional treatment group, regulating blood sugar level, dressing and traditional debridement; vacuum sealing drainage group, conventional treatment combined with the vacuum sealing drainage technology. The clinical efficacy of two treatments for diabetic foot was evaluated. RESULTS AND CONCLUSION: Compared with the traditional treatment group, the vacuum sealing drainage showed better outcomes in switching frequency, stable blood sugar control, preparation time, wound healing time and cure rate (P < 0.05). It indicates that the vacuum sealing drainage technology in the treatment of diabetic foot ulcers can resolute wound inflammation, stimulate the growth of granulation, create a favorable surgical condition for secondary skin grafting or flap coverage, significantly shorten the treatment time, and exhibit better curative effects than the traditional treatment. 中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程 全文链接： Figures and Tables | References | Related Articles | Metrics

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Clinical application of chitin medical wound dressing paste Zhang Jia, Hu Su-qin, Yan Lu, Wang Gui-ling 2014, 18 (34):  5555-5560.  doi: 10.3969/j.issn.2095-4344.2014.34.025 Abstract ( 1414 )   PDF (700KB) ( 599 )   Save BACKGROUND: The chitin medical wound dressing can relieve the wound pain, bleeding, and promote wound healing. It has good biocompatibility and antibacterial properties. With the good permeability and natural degradation in the body, it can be used clinically as a good biological wound dressing. OBJECTIVE: To evaluate the clinical outcome of the chitin medical wound dressing paste and routine dressings used in changing the dressing and medication. METHODS: We retrospectively studied 60 patients undergoing wound-treatment. They were divided into two groups: chitin medical wound dressing paste group and routine dressing group. Each group had 30 patients. We recorded wound healing rate, detection rate of bacteria, visual analog scale score, healing time and cost of treatment at 3, 7, 14 days after treatment. RESULTS AND CONCLUSION: The wound healing rate, detection rate of bacteria, visual analog scale score, healing time of the chitin medical wound dressing paste group were better than those of the routine dressing change medicine group (P < 0.05). But there was no difference in the cost of treatment between the two groups. Therefore, we can made the conclusion that the chitin medical wound dressing paste used in changing the dressing and medication can promote wound healing, reduce the antibacterial infection rate, and obtain better treatment satisfaction. 中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程 全文链接： Figures and Tables | References | Related Articles | Metrics

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Absorbable ligating clip in laparoscopic hysterectomy Li A-li 2014, 18 (34):  5561-5565.  doi: 10.3969/j.issn.2095-4344.2014.34.026 Abstract ( 341 )   PDF (603KB) ( 661 )   Save BACKGROUND: Titanium clip, commonly used in abdominal surgery and obstetrics and gynecology surgery, has a great damage to the vascular tissue, and its excessive clipping can cut off the vessel and produce artifacts and deflection MRI on MRI and CT scan. In addition, its biocompatibility is not well that increases the risks for local inflammatory response and scarring. OBJECTIVE: To observe the clinical efficacy of absorbable ligating clip in laparoscopic hysterectomy and to evaluate its clinical value. METHODS: Ninety-eight patients undergoing laparoscopic hysterectomy were randomly divided into two groups: 49 patients in the observation group were subjected to occlusion of the uterine artery by a Johnson absorbable ligating chip and then complete removal of the uterus; 49 patients in the control group were subjected to occlusion of the uterine artery with a titanium clip. Hospital stay and postoperative complications were recorded and analyzed between the two groups. RESULTS AND CONCLUSION: The use of absorbable ligating clip in laparoscopic hysterectomy significantly shortened the hospital stay, and postoperative pain, adhesions, pelvic fluid, fever and other complications were significantly reduced (P < 0.05). In addition, no postoperative infection occurred in the two groups and there was no significant difference in the class A healing rate between the two groups (P > 0.05). The results suggest that absorbable ligating clip with better biocompatibility can help to reduce tissue reactions and promote tissue repair, which is characterized as effective, safe and reliable. 中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程 全文链接： Figures and Tables | References | Related Articles | Metrics

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A meta-analysis of unilateral versus bilateral balloon kyphoplasty in the treatment of osteoporotic vertebral compression fractures Ma Gui-fu, Qian Yao-wen, Liu Lin, Luo Wen-yuan, Liu Shao-guang 2014, 18 (34):  5566-5571.  doi: 10.3969/j.issn.2095-4344.2014.34.027 Abstract ( 328 )   PDF (555KB) ( 368 )   Save BACKGROUND: Balloon kyphoplasty is effective in the treatment of osteoporotic vertebral compression fractures, but it is unclear that which one is proper, unilateral or bilateral approach, with better efficacy and fewer complications. OBJECTIVE: To assess the efficacy and safety of unilateral versus bilateral balloon kyphoplasty in the treatment of osteoporotic vertebral compression fractures. METHODS: We searched the electronic bibliographic databases including Cochrane Library, PubMed, EMBASE, ISI Web of Knowledge, CBMdisc and other databases to collect clinical trials concerning unilateral versus bilateral balloon kyphoplasty. Two estimators independently evaluated the quality of these included studies and analyzed data by Cochrane Collaboration’s RevMan 5.2 software. RESULTS AND CONCLUSION: Fourteen trials involving 876 patients were included. There were 442 cases of unilateral approach and 434 of bilateral approach. The meta-analysis showed that there were no significant differences in pain score by visual analog scale, vertebral height, and kyphotic angle; while the unilateral approach had less operating time, lower amount of cement injected and lower risk of cement leakage than the bilateral approach [mean difference (MD)=-19.33, 95% confidence interval (CI) (-24.42, -14.24); MD=-2.07, 95% CI (-2.42, -1.71); odds ratio=0.47, 95% CI (-24.42, -14.240)]. These findings indicate that the unilateral balloon kyphoplasty can reduce the leakage rate of bone cement. 中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程 全文链接： Figures and Tables | References | Related Articles | Metrics

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Tissue patch for prevention of dry socket after extraction of the mandibular molars: a sysrematic review Lu Qun, Huang Yi, Kong Ling-ying 2014, 18 (34):  5572-5576.  doi: 10.3969/j.issn.2095-4344.2014.34.028 Abstract ( 323 )   PDF (556KB) ( 345 )   Save BACKGROUND: Tissue patch is used to increase bone mass after mandibular molar extraction, which is conducive to late-stage repair, but it is unexpectedly found that after implantation of tissue patch, incidence of dry socket is significantly reduced. OBJECTIVE: To assess the efficacy of tissue patch for the control of dry socket caused by mandibular molar extraction via the method of systematic review. METHODS: MEDLINE (OVID), CENTRAL, EMBASE and CBM were searched for clinical randomized controlled trials and clinical controlled trials. The keywords were “dry socket, tissue patch, acellular dermis matrix, tooth extraction” in English and Chinese. The references of the included studies and 19 Chinese dental journals were hand-searched. Two reviewers independently assessed the risk of bias using Cochrane Collaboration’s tool, and extracted data. Meta-analysis was delivered with Revman 5.1. RESULTS AND CONCLUSION: Eight studies, including five randomized controlled trials and three clinical controlled trials, were included. Totally 2 052 participants were involved. Seven of the included studies had moderate risk of bias and one had high risk of bias. Meta analysis showed that implantation of the tissue patch into the extraction socket could reduce 86% of the risk of dry socket (relative risk=0.14, 95% confidence interval [0.08, 0.26], P < 0.000 01). Sensitivity analysis showed that this outcome was relatively stable. Implantation of tissue patch into extraction socket could significantly reduce the risk of dry socket, but more randomized controlled trials are needed. 中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程 全文链接： Figures and Tables | References | Related Articles | Metrics