Synergistic effect of high mobility group protein B1 on calcium phosphate-induced release of inflammatory cytokines from macrophages

doi:10.3969/j.issn.2095-4344.2014.33.012

Abstract

Abstract:

BACKGROUND: More and more evidence suggests that macrophages and inflammation reactions are involved in the formation and development of nephrolithiasis. Previous studies have found that calculi crystals can stimulate macrophages to release high mobility group protein B1.
OBJECTIVE: To investigate the synergistic effect of high mobility group protein B1 in calcium phosphate induced release of interleukin-1β, interleukin-6, tumor necrosis factor α and monocyte chemotactic factor 1 from human macrophages.
METHODS: (1) The induced U937 cells were respectively stimulated with RPMI (blank), 100 mg/L calcium phosphate, 100 μg/L high mobility group protein B1 and 100 mg/L calcium phosphate+100 μg/L high mobility group protein B1 for 1, 2 and 4 hours to collect cell supernatant. (2) The induced U937 cells were respectively stimulated with 100 mg/L calcium phosphate, 100 mg/L calcium phosphate+10 μg/L high mobility group protein
B1, 100 mg/L calcium phosphate+50 μg/L high mobility group protein B1, 100 mg/L calcium phosphate+100 μg/L high mobility group protein B1 for 4 hours to collect cell supernatant. Levels of interleukin-1β, interleukin-6, tumor necrosis factor α and monocyte chemotactic factor 1 were determined by ELISA.
RESULTS AND CONCLUSION: The levels of interleukin-1β, interleukin-6, tumor necrosis factor α and monocyte chemotactic factor 1 in the cell culture supernatant of 100 mg/L calcium phosphate group and 100 μg/L high mobility group protein B1 group were both higher than those in the blank group in a time-dependent manner (P < 0.05). The levels of interleukin-1β, interleukin-6, tumor necrosis factor α and monocyte chemotactic factor 1 in the cell culture supernatant of different concentrations of high mobility group protein B1 groups were all higher than those in the
100 mg/L calcium phosphate group in a concentration-dependent manner (P < 0.05). The results suggest that both calcium phosphate and high mobility group protein B1 can induce the release of interleukin-1β, interleukin-6, tumor necrosis factor α and monocyte chemotactic factor 1 from human macrophages and the high mobility group protein B1 has the synergistic effect with calcium phosphate to induce interleukin-1β, interleukin-6, tumor necrosis factor α and monocyte chemotactic factor 1 from human macrophages.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

全文链接：

Key words: kidney calculi, high mobility group proteins, interleukin-1beta, interleukin-6, tumor necrosis factor-alpha, macrophages

CLC Number:

R318

Feng You-cai, Deng Yao-liang, Tao Zhi-wei, Wang Xiang, Li Cheng-yang, Huang Peng, Wu Bo. Synergistic effect of high mobility group protein B1 on calcium phosphate-induced release of inflammatory cytokines from macrophages [J]. Chinese Journal of Tissue Engineering Research, 2014, 18(33): 5317-5322.

Figures/Tables 6

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