Interleukin-10 and conjugative plasmid of Salmonella mediate bacterial biofilm formation

doi:10.3969/j.issn.2095-4344.2014.33.011

Abstract

Abstract:

BACKGROUND: Previous studies discovered that pRST98, originally isolated from Salmonella enterica serovar typhimurium (S.typhimurium) could promote bacterial biofilm formation. In addition, bacterial harboring pRST98 can promote the secretion and expression of interleukin-10 after infection in cells and animals.
OBJECTIVE: In vitro studies have discovered the effects of interleukin-10 at varying concentrations and conjugative plasmid pRST98 on the biofilm formation of S.typhimurium.
METHODS: S.typhimurium wild-type strain χ3306, virulence plasmid-deletion S.typhimurium strain χ3337 and pRST98-transconjugant S.typhimurium χ3337/pRST98 were established in vitro and cultured for biofilm formation. 1, 10, 100 µg/L interleukin-10 were added during the biofilm formation. 0 µg/L interleukin-10 was set as a control. Crystal violet staining method, semi-quantitative method, confocal laser scanning microscopy and scanning electron microscopy were used to determine the effects of interleukin-10 on the biofilm formation and compare the effects of S.typhimurium with or without pRST98.
RESULTS AND CONCLUSION: Intra-group comparison showed that, compared with the control group, S.typhimurium gathered together and formed thicker biofilm in concentration of 1 and 10 µg/L of interleukin-10. The promotion effects of S.typhimurium on biofilm formation were greatly improved in 10 µg/L. Interleukin-10 in 100 µg/L inhibited S.typhimurium biofilm formation. Inter-group comparison showed that, A570 in χ3337/pRST98 was greatly higher than that in χ3306 and χ3337 under the same concentration of interleukin-10. The results indicate that both 1 and 10 µg/L of interleukin-10 promote biofilm formation, especially bacteria harboring pRST98.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

全文链接：

Key words: interleukin-10, biofilms, Salmonella typhimurium

CLC Number:

R318

Que Feng-xia, Liu Zhen, Wang Ting, Yan Jing, Li Yuan-yuan, Wu Shu-yan, Huang Rui. Interleukin-10 and conjugative plasmid of Salmonella mediate bacterial biofilm formation[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(33): 5310-5316.

Figures/Tables 5

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