Chinese Journal of Tissue Engineering Research ›› 2019, Vol. 23 ›› Issue (6): 901-905.doi: 10.3969/j.issn.2095-4344.1579

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Construction and biocompatibility of blood biofilms based on electrospinning technology

Sun Yu1, 2, 3, Zou Qiang1, 2, 3, Li Xuanze1, 2, 3, Wu Zhanyu1, 2, Yang Long2, Wang Jianji2, Liu Qin4, Ma Minxian4, Ye Chuan1, 2, 3   

  1. 1Guizhou Medical University, Guiyang 550004, Guizhou Province, China; 2Department of Orthopedics, Affiliated Hospital of Guizhou Medical University, Guiyang 550004, Guizhou Province, China; 3National and Guizhou Joint Engineering Laboratory for Cell Engineering and Biomedicine Technique, Guiyang 550004, Guizhou Province, China; 4Department of Prosthodontics, Affiliated Stomatology Hospital of Guizhou Medical University, Guiyang 550004, Guizhou Province, China
  • Received:2018-09-13 Online:2019-02-28 Published:2019-02-28
  • Contact: Ye Chuan, Chief physician, Guizhou Medical University, Guiyang 550004, Guizhou Province, China; Department of Orthopedics, Affiliated Hospital of Guizhou Medical University, Guiyang 550004, Guizhou Province, China; National and Guizhou Joint Engineering Laboratory for Cell Engineering and Biomedicine Technique, Guiyang 550004, Guizhou Province, China
  • About author:Sun Yu, Master candidate, Physician, Guizhou Medical University, Guiyang 550004, Guizhou Province, China; Department of Orthopedics, Affiliated Hospital of Guizhou Medical University, Guiyang 550004, Guizhou Province, China; National and Guizhou Joint Engineering Laboratory for Cell Engineering and Biomedicine Technique, Guiyang 550004, Guizhou Province, China
  • Supported by:

    the National Natural Science Foundation of China, No. 81360232 (to YC); the Science and Technology Innovation Team Funded Project of Guiyang Municipal Science and Technology Department, No. 20175-17 (to YC)

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Abstract:

BACKGROUND: Previous studies have shown that fibrinogen can serve as tissue engineering scaffold material. Therefore, the reuse of fibrinogen in the autologous blood can make the scaffold material which has non-immunogenicity and good biocompatibility.

OBJECTIVE: To explore the method of preparing blood biofilm using the electro-spinning technology and to evaluate the biocompatibility of the fibrinogen scaffold.
METHODS: Fibrinogen was purified out of the fresh blood after the procedures of separation, centrifugation, and purification, and then subjected to vacuum freeze drying (group A), low temperature spray drying (group B), and oven drying at 56 oC (group C). It was finally made into fiber film using the electrospinning technology. Contact angle of the three sets of scaffold materials was detected, and the three-dimensional structure of the scaffolds was observed under scanning electron microscope. Bone marrow mesenchymal stem cells were co-cultured with the three sets of scaffolds in vitro. Cell growth was observed using scanning electron microscope at 7 days of culture, and cell proliferation in the group B was detected using Alamar Blue kit at 1-4 days of culture.

RESULTS AND CONCLUSION: (1) Under the scanning electron microscope, fiber structure with uneven fiber thickness was observed, and a lot of droplet-like structures were also visible in the group A; the ordered multi-layer fiber structure with similar fiber diameter and pore size was observed in the group B, and only a small amount of droplet-like structures were detected; in the group C, there was no fiber structure, but the droplet-like structures of different sizes. (2) The contact angle of the scaffold material was (82±3)o in the group A, (67±5)o in the group B, and (80±3)o in the group C. (3) After 7 days of co-culture, the bone marrow mesenchymal stem cells adhered to the scaffold materials in the three groups. The cells on the surface of group B were evenly distributed, which were adherent to the fiber surface and had relative regular nuclei. Only a small amount of cells grew onto the scaffolds in the groups A and C, especially in the group C, in which the cells had irregular nuclei and no fiber structure was observed. (4) The cells in the group B exhibited an increasing proliferation tendency as shown by the results of Alamar Blue experiment, and the cell growth was in good status. All these findings indicate that it is feasible to prepare the blood fibrinogen biofilm with good biocompatibility using the electrospinning technology. 

Key words: Fibrinogen, Biofilms, Blood;, Electrospinning, Tissue Engineering

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