Influence of different wear particles on human peripheral blood mononuclear cells in vitro

doi:10.3969/j.issn.2095-4344.1572

Abstract

Abstract:

BACKGROUND: After artificial joint replacement, daily activities lead to the production of a large number of titanium 6 aluminium 4 vanadium (Ti6Al4V), polymethylmethacrylate (PMMA), ultra-high-molecular-weight-polyethylene (UHMWPE) and chromium-cobalt alloy (Co-Cr) particles, activating monocytes to release a large number of inflammatory cytokines and chemokines, including a series of biological cascade reactions, such as inducing bone dissolution and forming pseudo-granuloma, and eventually leading to aseptic loosening of the prosthesis.

OBJECTIVE: To investigate the effect of Ti6Al4V, PMMA, UHMWPE and Co-Cr particles on tumor necrosis factor-α, interleukin-1 and interleukin-6 in the human peripheral blood monocytes cultured in vitro and to explore the underlying mechanism of aseptic loosening of the prosthesis by inducing bone dissolution.

METHODS: Human peripheral blood mononuclear cells were cultured with Ti6Al4V, PMMA, UHMWPE and Co-Cr particles at different concentrations. After 72 hours of culture, ELISA technique was utilized to quantify the secretion of different cytokines includes tumor necrosis factor-α, interleukin-1 and interleukin-6. We observed the influence of different wear particle concentrations and particle properties on the viability of human peripheral blood mononuclear cells.

RESULTS AND CONCLUSION: Cell viability exceeded 95% for all groups, except for 87% in the Co-Cr group when the particle/cell ratio was

1 000. Compared with the naive mononuclear cells (particle/cell ratio of 0), the expression of tumor necrosis factor-α, interleukin-1 and interleukin-6 in the activated mononuclear cells increased significantly when the particle/cell ratio was 10, 100 and 1 000 (P < 0.05). Ti6Al4V, PMMA and UHMWPE particles stimulated human mononuclear cells to overexpress tumor necrosis factor-α, interleukin-1 and interleukin-6, which were positively correlated with the concentration of particles. However, when the Co-Cr particle/cells ratio was 1 000, the expression levels of tumor necrosis factor-α, interleukin-1 and interleukin-6 were decreased. During the osteolysis reaction, Ti6Al4V particles had strongest biological activity, followed by Co-Cr and PMMA particles, and the biological activity of UHMWPE particles was lowest. Ti6Al4V, PMMA, UHMWPE and Co-Cr particles showed dose-dependent effects on the human peripheral blood mononuclear factors during the osteolysis.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Arthroplasty, Replacement, Particulate Matter, Monocytes, Tumor Necrosis Factor-alpha, Interleukin-1, Interleukin-6, Tissue Engineering

CLC Number:

R459.9

Li Peng, Yang Shuye, Zhang Kai, Jia Long, Du Gangqiang, Liu Dong, Zhang Xinjun, Zhang Degang, Wang Zhigang. Influence of different wear particles on human peripheral blood mononuclear cells in vitro[J]. Chinese Journal of Tissue Engineering Research, 2019, 23(6): 894-900.

Figures/Tables 9

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