Chinese Journal of Tissue Engineering Research ›› 2015, Vol. 19 ›› Issue (43): 6993-6997.doi: 10.3969/j.issn.2095-4344.2015.43.020

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The cleaning of plaque biofilm on the surface of macromolecule ocular prosthesis material

Guo Su-ping1, Song Yan2, Wang Fan-tao2, Xu Xin2, Jia Wei1   

  1. 1Weifang Medical University, Weifang 261053, Shandong Province, China; 2Department of Stomatology, Affiliated Hospital of Weifang Medical University, Weifang 261031, Shandong Province, China
  • Received:2015-07-20 Online:2015-10-15 Published:2015-10-15
  • Contact: Song Yan, Chief physician, Professor, Department of Stomatology, Affiliated Hospital of Weifang Medical University, Weifang 261031, Shandong Province, China
  • About author:Guo Su-ping, Studying for master’s degree, Weifang Medical University, Weifang 261053, Shandong Province, China

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Abstract:

BACKGROUND: The micro-ecological environment has been broken when the ocular prosthesis was inset into the conjunctival sac. The recede of self cleaning function is more conducive to the microbial growth and colonization. The cleaning of plaque biofilm on ocular prosthesis surface affects the patient's wearing comfort and quality of life. It is necessary to seek an effective cleaning method.

OBJECTIVE: To compare the clearance effect of five cleaning methods on the palque biofilm on ocular prosthesis surface.
METHODS: The conjunctival secretions from 84 patients who were subjected to ocular prosthesis repair were taken for bacterial culture and identification. Fifty pieces of self-curing resin and thermosetting resin artificial eyes were produced. The artificial eyes in each group were randomly divided into five groups, and were cleaned respectively with clear water, volume fraction of 75% ethanol, Boston SIMPLUS, polident and toothpaste. After the completion of the cleaning, the test piece was conducted residual biofilm culture. The clearance effects of different processing modes were evaluated using colony counting method.

RESULTS AND CONCLUSION: Eighty-four specimens were submitted for inspection, of which 49 were positive. The Staphylococcus aureus separation rate was 14.29%. Staphylococcus epidermidis separation rate accounted for 13.10%. Maxwell Corynebacterium separation rate accounted for 7.14%. When water, Boston SIMPLUS and toothpaste were used for cleaning, the Staphylococcus aureus colony number in the self-curing resin group was higher than that in the thermosetting resin group (P < 0.05); when ethanol and polident were used for cleaning, there was no difference in the Staphylococcus aureus colony number between these two groups. In self-curing resin, the colony count in the clear water treatment group was higher than that in the other treatment groups (P < 0.05). The colony count in the ethanol treatment group was lower than that in the Boston SIMPLUS group (P < 0.05). There was no significant difference in the colony count between other groups. In thermosetting resin, the colony count in the clear water treatment group was higher than that in the other treatment groups (P < 0.05). There was no significant difference in the colony count between other groups. These results demonstrate that ethanol, Boston SIMPLUS, polident and toothpaste have better cleaning effects on Staphylococcus aureus biofilms on the surface of two kinds of ocular prostheses than the clear water rinse. Overall, it is encouraged to clean the artificial eyes using polident and Boston SIMPLUS, in order to avoid the occurrence of microbial infection in the conjunctival sac after wearing ocular prosthesis.
中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Eye, Artificial, Biofilms, Methylmethacrylates, Tissue Engineering