Effect of interleukin-10 gene on liver pancreatic and duodenal homeobox 1 expression in nonobese diabetic mice

doi:10.3969/j.issn.2095-4344.2015.27.020

Abstract

Abstract:

BACKGROUND: Studies have found that liver cells can synthesize insulin after giving pancreatic and duodenal homeobox 1 (PDX1) gene. Anti-CD20 monoclonal antibody can inhibit the immune reaction of insulin-producing liver cells, but the mechanism is unclear.
OBJECTIVE: To observe the influence of interleukin-10 gene on liver cells and liver PDX1 expression in nonobese diabetic mice after interfered by adenovirus vector-mediated murine interleukin-10 and anti-CD20 monoclonal antibody.
METHOD: Forty nonobese diabetic female mice aged 3-5 weeks were randomly divided into anti-CD20, anti-CD20 + interleukin-10, interleukin, and control groups. Mice in each group were respectively injected with anti-CD20 monoclonal antibody, anti-CD20 monoclonal antibody + adenovirus vector-mediated murine interleukin-10, adenovirus vector-mediated murine interleukin-10 and normal saline on days 1, 8, 15 and 21 via tail vein.
RESULTS AND CONCLUSION: At 12 weeks, the blood glucose level of mice treated with anti-CD20 monoclonal antibody and/or interleukin-10 was significantly reduced compared with the control group, while the insulin, interleukin-10 and CD20 expression levels in the serum and liver were significantly increased, the liver PDX1 expression was also upregulated. Anti-CD20 monoclonal antibody with interleukin-10 had more obvious effects than the single use. No matter the combined intervention or single use, anti-CD20 monoclonal antibody and interleukin-10 show no impact on the inflammation of liver cells. Anti-CD20 monoclonal antibody and/or interleukin-10 increases PDX1 expression in nonobese diabetic mice.

中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

Key words: Diabetes Mellitus, Type 1, Antigens, CD20, Interleukin-10, Liver

CLC Number:

R318

Yu Shu-feng, Ren An-xia, Zhang Li-juan, Li Tang. Effect of interleukin-10 gene on liver pancreatic and duodenal homeobox 1 expression in nonobese diabetic mice [J]. Chinese Journal of Tissue Engineering Research, 2015, 19(27): 4371-4378.

Figures/Tables 9

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