Subcutaneous embedment of the acellular urethra in rats

doi:10.3969/j.issn.2095-4344.2014.33.014

Abstract

Abstract:

BACKGROUND: The nature of graft rejective reaction is the antigen immunological reaction of recipient’s immune system in response to donor’s graft.
OBJECTIVE: To observe the effect of chemical extraction on the immunogenicity of acellular peripheral urethral isografts.
METHODS: Twenty urethras of Sprague-Dawley rats, 1 cm long, were included in this study. Ten urethras were treated with chemical acellular method to remove immunogenic component, thus prepared into acellular urethras; another ten urethras were fresh, without any acellular treatment. Acellular urethras were implanted into the back of ten Wistar rats, while fresh urethras into another ten rats. 6 weeks after transplantation, the specimens were harvested for gross and histological observations.
RESULTS AND CONCLUSION: After acellular or fresh urethras were implanted into the rats, no autotomy was observed, the incision healed well, the diet and activities of the rat were normal, and no ulcer formation was found. At 6 weeks, tissue membrane formed around the acellular urethra, while no formation was visible around fresh urethra by gross observation. Hematoxylin-eosin staining showed that, acellular urethras were gradually absorbed and replaced by smooth muscular tissue. And unequal blood vessels grew in the acellular urethra, even formed blood sinusoid. In the control group, the urethras were not absorbed and no blood vessels were grown. The acellular urethras can gradually form normal urethra in rats, indicating that the acellular urethral autograft has a lower antigenicity and can replace the cavernous body of normal urethra.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

全文链接：

Key words: urethra, transplantation, homologous, immunity

CLC Number:

R318

Liu Xiao-yan, Wang Yuan-zheng, Li Ying, Li Lin, Mao Dan, Li Hang-xu. Subcutaneous embedment of the acellular urethra in rats[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(33): 5329-5333.

Figures/Tables 3

References

[1]De Filippo RE,Yoo JJ,Atala A.Urethral replacement using cell seeded tubularized collagen matrices.J Urol. 2002 ;168(4Pt2); 1644-1768.
[2]Figallo E,Flaibani M,Zavan B,et al. Micropatterned biopolymer 3D scaffold for static and dynamic culture of dynamic culture of human fibroblast .Biotechnol Prog. 2007; 23(1):209-212.
[3]Campodonico F,Benelli Michelazzi A,et al.Bladder cell culture on small intestinal submucosa as bioscaffod:experimental study on engineeredurothelial grafts.Eur Urol. 2004;46(4): 531-507.
[4]Wunsch L,Ehlers EM, Russlies M.Matrix testing for urothelial tissue engineering.Eur J Pediatr SurgJT. 2005; 15(3):164-169.
[5]Stack A,Hayward SW,Baskin LS,et al.Mqlecular, cellular and developmental biology of urothelium as a basis of bladder regeneration .Differentiation.2005;73(4):121-103.
[6]Niu YS,Zhang WX,Li HC,et al.Carbon dioxide/propylene oxide coupling reaction catalyzed by chromium salen complexes. Polymer.2009;50(2):441-446.
[7]Wang Jintao,Zhu Quan,Lu Xialian,et al.ZnGA–MMT catalyzed the copolymerization of carbon dioxide with propylene oxide. Eur Polym J.2005; 41(5):1108-1114.
[8]Zhou X, Yang XG, Chen T,et al.Synthesis of Propylene Carbonate from Carbon Dioxide and o-Chloropropanol. Chinese Journal of Catalysis.2009;30(1):7-8.
[9]Wang XL, Li RKY, Cao YX, et al. Essential work of fracture analysis for starch filled poly(propylene carbonate) composites.Materials & Design.2007;28(6):1934-1939.
[10]Bartsch GJ,Atala A.Tissue engineering in urology.Basic principles and application.UrologeA.2003;42(3);255-364.
[11]Lakshmanan Y,Frimberger D,Gearhart JD,et al.Human embryoid body-derived stem cells in co-culture with bladder smooth muscle and urothelium.Urology.2005;65(4):821-826.
[12]Liu CX,Lin YY,LiHL,et al .Application of silk fibroin flim for repairing rabbit urethral defect .Nan Fang Yi Ke Da Xue Xue Bao. 2007;27(2):182-185
[13]Moradzadeh A, The impact of motor and sensory nerve architecture on nerve regeneration. Exp Neuro.2008;212(2): 370-376.
[14]Atala A,Cima LG,KimWet,et al.Injectable alginate seeded with chondrocytes as apotential treatment for vesicouertal reflux. Urol.2009;150 (3):734-749.
[15]Moradzadeh AL. The impact of motor and sensory nerve architecture on nerve regeneration. Exp Neurol.2008;212(2): 332-334.
[16]Walsh PC, Retik AB ,Vaughan ED,et al.Camp bellps urology. USA:An Imprint of Elsevier science.2002;70(4):243-245.
[17]Henry GD,Wilson SK.UPdates in inflatable penile prostheses.Urol Clin North Am.2007;34(7):534-553.
[18]Simonsen U,Prieto,Hernandez M,et al. Adrenoceptor- mediated regulation of the contractility in horse penile resistance arteries.JVasc Res.2007;34(6):89-111.
[19]Eltahawy EA,VIrasoro P, Schlossber SM.Long2 tem follow up for excision and primary a nastomosis for nteriorurethral stricture.JUrol.2007;177(5):1703-1801.
[20]Bazo A, Rao K. Urethral strictures:once a stricture always a striure.trop Doct. 2003;33(4):68-70.
[21]Saraste A,PulkkiK,Kallajokin M,et al.Apoptosis in human acute myocardial infarction.Circulation. Circulation. 1997; 95(2):320-323.
[22]Yuan H, Pu C, Wei Q,et al.Research progress of extracellular matrix material for tissue engineering.Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi.2012;26(10):1251-1254.
[23]Jiang H, Ma LM, Zhou J,et al.Human foreskin acellular matrix graft: a good scaffold for urethral tissue engineering. Zhonghua Nan Ke Xue. 2009;15(5): 409-412.
[24]Han P, Song C, Yang YR,et al.Urethral acellular matrix graft for repairing urethral defect in rabbits.Nan Fang Yi Ke Da Xue Xue Bao.2009;29(1):124-127, 132.
[25]Li C, Xu YM, Song LJ,et al. Urethral reconstruction using oral keratinocyte seeded bladder acellular matrix grafts.J Urol. 2008; 180(4):1538-1542.
[26]Fu Q, Deng CL, Liu W, et al.Urethral replacement using epidermal cell-seeded tubular acellular bladder collagen matrix.BJU Int. 2007;99(5):1162-1165.
[27]Bernoulli J,Yatkin E,Talvitie EM,et al.Urodynamic changes in a noble rat model for nonbacterial prostatic inflammation. Prostate.2007;67(8):865-870.