Loading...

Table of Content

    06 August 2014, Volume 18 Issue 32 Previous Issue    Next Issue
    For Selected: Toggle Thumbnails
    Role of Spry1 in osteogenic differentiation of human bone marrow mesenchymal stem cells under miR-21 regulation
    Yang Nan, Zhou Wei, Wang Guang, Ding Yin, Jin Yan
    2014, 18 (32):  5085-5089.  doi: 10.3969/j.issn.2095-4344.2014.32.001
    Abstract ( 353 )   PDF (705KB) ( 506 )   Save

    BACKGROUND: Previous studies have found that miR-21 expression is increased during osteogenic differentiation of bone marrow mesenchymal stem cells, but the action and molecular mechanism of miR-21 are still unclear.
    OBJECTIVE: To verify the target gene of miR-21, Spry1, and to explore the role of Spry1 in osteogenic differentiation of human bone marrow mesenchymal stem cells.
    METHODS: Luciferase report was used to verify Spry1 gene targeted by miR-21, and western blot assay was used to detect the expression of Spry1 in the osteogenesis of human bone marrow mesenchymal stem cells. Spry1 expression vector was established and transfected into human bone marrow mesenchymal stem cells. Osteogenesis ability of human bone marrow mesenchymal stem cells was analyzed after Spry1 high expression by alkaline phosphatase, alizarin red staining, RT-PCR and western blot.
    RESULTS AND CONCLUSION: Luciferase report suggested that Spry1 was a target gene of miR-21. The expression level of Spry1 was decreased in the osteogenesis of human bone marrow mesenchymal stem cells. Increasing expression of Spry1 could inhibit osteogenic differentiation of human bone marrow mesenchymal stem cells. These results indicate that Spry1 as a target gene of miR-21 negatively regulates osteogenic differentiation 
    of human bone marrow mesenchymal stem cells, and plays an important role in bone formation process.


    中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程


    全文链接:

    Figures and Tables | References | Related Articles | Metrics
    Mangiferin protects bone marrow mesenchymal stem cells against hypoxia
    Cheng Jian-wen, Zhao Jin-min, Li Xiao-feng, Tan Zhen
    2014, 18 (32):  5091-5096.  doi: 10.3969/j.issn.2095-4344.2014.32.002
    Abstract ( 253 )   PDF (658KB) ( 687 )   Save

    BACKGROUND: Bone marrow mesenchymal stem cells have particularly applied prospects in tissue engineering. However, the death of transplanted cells limits the tissue regeneration. To search a new drug of anti-free radicals and protecting bone marrow mesenchymal stem cells is of great significance.
    OBJECTIVE: To investigate the protective effects of mangiferin on bone marrow mesenchymal stem cells against hypoxia.
    METHODS: Rat bone marrow mesenchymal stem cells were cultured in vitro and hypoxia cell model was established by cobalt chloride. Cells were divided into normal control group, hypoxia group (treated with cobalt chloride), and mangiferin groups (cobalt chloride+20, 40, 80, 160 µmol/L mangiferin). After 12 and 24 hours of hypoxia, superoxide dismutase, malondialdehyde, and catalase levels in the cell supernatant were determined. After 3, 6, 12, 24 hours of hypoxia, reactive oxygen species change was detected in each group.
    RESULTS AND CONCLUSION: Mangiferin significantly improved the survival rate of bone marrow mesenchymal stem cells exposed to hypoxia, increased the intracellular superoxide dismutase and catalase activities, decreased intracellular malondialdehyde and reactive oxygen species levels, thereby effectively protecting bone marrow mesenchymal stem cells against hypoxia. These findings indicate that mangiferin has effective protection against hypoxia and strong antioxidant ability, and can significantly reduce oxidative damage.


    中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程


    全文链接:

    Figures and Tables | References | Related Articles | Metrics
    Construction of bladder tissue-engineered grafts by urothelium-induced bone marrow mesenchymal stem cells and bladder acellular matrix
    Xiong Yun-he, Yang Si-xing, Meng Ling-chao, Liao Wen-biao, Song Chao
    2014, 18 (32):  5097-5012.  doi: 10.3969/j.issn.2095-4344.2014.32.003
    Abstract ( 228 )   PDF (2239KB) ( 446 )   Save

    BACKGROUND: Urothelial cells are important seeding cells for urinary tissue engineering, but they are difficult to proliferate in vitro. Several studies have shown that bone marrow mesenchymal stem cells can differentiate into urothelial cells, but how these cells functions in vivo in epithelium generation after implantation, and the application of these cells in tissue engineering, are rarely studied.
    OBJECTIVE: To explore the isolation and proliferation of rabbit bone marrow mesenchymal stem cells that are induced into urothelial cells in combination with rabbit bladder acellular matrix to construct tissue-engineered grafts, and to assess the effect of the induced cells as seeding cells.
    METHODS: Twelve 8-week-old male New Zealand white rabbits were chosen to obtain bone marrow samples through tibia puncture, and to isolate bone marrow mesenchymal stem cells by density gradient centrifugation. Then the fourth or fifth generation of bone marrow mesenchymal stem cells were cultured in conditioned medium for 2 weeks, and then identified by PCR and immunofluorescence. After that, the induced cells were seeded on rabbit bladder acellular matrix to construct tissue-engineered grafts for bladder repairing. Another 12 rabbits 
    served as control group, and urothelial cells combined with bladder acellular matrix was used for bladder repairing.
    RESULTS AND CONCLUSION: Bone marrow mesenchymal stem cells were successfully cultured and proliferated in vitro. After induction, PCR detection suggested that stem cell marker (CD44) expression decreased, and epithelial cell marker (UP1a) expression increased in the induced cells. Immunofluorescence staining demonstrated that the induced cells rather than bone marrow mesenchymal stem cells were positive for specific urothelial marker, UP1a. A stable continuous epithelial layer was observed on tissue-engineered grafts constructed by induced cells after 2 weeks, similar to the grafts built by urothelial cells. Induced bone marrow mesenchymal stem cells can differentiate into urothelial cells that can be used as seeding cells for urinary tissue engineering, which may be another choice out of urothelial cells.

    Figures and Tables | References | Related Articles | Metrics
    Isolation and culture methods of bone marrow stromal stem cells from the skull
    Ding Dong, Liang Jun, Zhang Ji-fei
    2014, 18 (32):  5103-5107.  doi: 10.3969/j.issn.2095-4344.2014.32.004
    Abstract ( 285 )   PDF (1640KB) ( 437 )   Save

    BACKGROUND: PA6 cells are bone marrow stromal stem cells from the mouse skull, and scientists have found that PA6 cells co-cultured with some kinds of stem cells have shown neural differentiation, based on which, PA6 cells can be used for repair of nerve injury. Therefore, researchers give more and more attentions to PA6, but few studies have addressed isolation and culture methods of bone marrow stromal stem cells from the skull.
    OBJECTIVE: To isolate and culture bone marrow stromal stem cells from the skull of Sprague-Dawley rats and to observe cellular morpholopy in vitro and perform immunofluorescence identification.
    METHODS: Under sterile conditions, newborn Sprague-Dawley rat’s skull was cut into pieces. Small skull pieces were washed using Dulbecco’s modified Eagle’s medium containing 10% fetal bovine serum. Single cell suspension was made, and placed in culture flask. The culture medium was changed many times for cell purification. The second passage of cells was obtained for morphology observation under an inverted microscope. Cell surface markers were detected by using immunofluorescence staining.
    RESULTS AND CONCLUSION: After the primary culture for 24 hours, cells exhibited adherent growth; after 3 days, cells were increased in number, and presented with irregular shapes, such as polygon, triangle, spindle and flat shape. After passage, cells were uniform in morphology, arranged in radial and bunch patterns, and exhibited strong adherent ability. Some cells grew in cluster, and proliferated faster than primary cells. Immunofluorescence 
    staining showed that cells were positive for CD105, CD73, CD44, CD90, but negative for CD45, D34,CD14, HLA-DR. Results indicate that this method can obtain bone marrow stromal stem cells.


    中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程


    全文链接:

    Figures and Tables | References | Related Articles | Metrics
    Bone marrow mesenchymal stem cells from Sprague-Dawley rats: aging inhibits cell proliferation and differentiation
    Cui Xin-tao, Tao Shu-qing
    2014, 18 (32):  5108-5113.  doi: 10.3969/j.issn.2095-4344.2014.32.005
    Abstract ( 316 )   PDF (2760KB) ( 382 )   Save

    BACKGROUND: Bone marrow mesenchymal stem cells are ideal as tissue engineering seed cells, but the proliferation and differentiation of mesenchymal stem cells in vitro is very different at different ages. Moreover, there are few reports on the association between age and the number of bone marrow mesenchymal stem cells.
    OBJECTIVE: To observe the difference in differentiation ability of bone marrow mesenchymal stem cells from rats at different ages.
    METHODS: We isolated, purified and amplified the mesenchymal stem cells from rat bone marrow in vitro by the whole bone marrow adherent culture; observed the morphological characteristics of mesenchymal stem cells under an inverted phase contrast microscope; detected cell surface markers by flow cytometer. Then, mesenchymal stem cells were induced in vitro into osteoblasts and chondroblasts and verified. Passage 3 cells from rats at ages of 2, 4, 6, 8, 12 weeks and 10, 12 months were subjected to osteogenic induction at weeks 1, 2, 3. ELISA was used to determine osteocalcin content.
    RESULTS AND CONCLUSION: Bone marrow mesenchymal stem cells cultured in vitro were adherent and exhibited a fibroblast-like spindle shape. In vitro, cells proliferated quickly to form colonies. Flow cytometry showed that the cells were positive for CD29, CD90, but negative for CD45, and partially expressed CD44. After osteogenic induction, cells were positive for alkaline phosphatase staining and alizarin red staining; after chondrogenic induction, cells were positive for alcian blue staining. Mesenchymal stem cells could be isolated and cultured by the method of bone marrow adherent culture in vitro. However, bone marrow mesenchymal stem cells from rats at different ages exhibit decreased proliferation and differentiation abilities with the increase of age through determination of osteocalcin content.


    中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程


    全文链接:

    Figures and Tables | References | Related Articles | Metrics
    Biological characteristics of umbilical cord mesenchymal stem cells in different conditioned media
    Zhou Jian-yu, Xu Yue, Huang Wen-jing, Liu Jun-jiang, Hong Jing-xin
    2014, 18 (32):  5114-5119.  doi: 10.3969/j.issn.2095-4344.2014.32.006
    Abstract ( 222 )   PDF (791KB) ( 375 )   Save

    BACKGROUND: The growth of mesenchymal stem cells in vitro in different conditioned media is different evidently. So it is necessary to choose a more suitable medium.
    OBJECTIVE: To contrast and observe the proliferation of human umbilical cord mesenchymal stem cells in three kinds of media and to check the immunophenotype and differentiation ability of mesenchymal stem cells.
    METHODS: Human umbilical cord mesenchymal stem cells were collected by explant method in sterile conditions. After subculturing by T75 incubation bottles, the third generation of mesenchymal stem cells were cultured in Dulbecco’s modified Eagle’s medium/Ham’s nutrient mixture F-12 medium containing 5% fetal bovine serum, Dulbecco’s modified Eagle’s medium/Ham’s nutrient mixture F-12 containing 10% fetal bovine serum and Mesen PRO RSTM medium. After 1, 3, 5, 7 days of culture, the cells were counted to draw a growth curve. Immunophenotype of the third generation of umbilical cord mesenchymal stem cells were determined by flow cytometry and the ability of osteogenic and adipogenic differentiation was also detected.
    RESULTS AND CONCLUSION: The third generation of cells cultured highly expressed CD44, CD73, CD90, CD105, but did not express CD29, CD31, CD34, HLA-DR. The oil red O staining showed a lot of little red lipid drops after adipogenic induction; alizarin red staining showed osteoblast-like cells after osteogenic induction, indicating umbilical cord mesenchymal stem cells in vitro have the potential of multi-directional differentiation. After observation and counting, the colony and shape of cells cultured in Dulbecco’s modified Eagle’s medium/Ham’s nutrient mixture F-12 containing 10% fetal bovine serum were superior to those cultured in the other two media. Therefore, it is concluded that Dulbecco’s modified Eagle’s medium/Ham’s nutrient mixture F-12 containing 10% fetal bovine serum is preferred for cell subculture.


    中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程


    全文链接:

    Figures and Tables | References | Related Articles | Metrics
    Interferon-gamma enhances immunosuppression mediated by adipose-derived stem cells
    Yiliyaer Yilihamu, Wang Yun-hai, Wang Li, Xu Xin-cai, Aimulaguli Abula, Zhang Wen-bin
    2014, 18 (32):  5120-5125.  doi: 10.3969/j.issn.2095-4344.2014.32.007
    Abstract ( 316 )   PDF (749KB) ( 499 )   Save

    BACKGROUND: Adipose stem cells can differentiate into bone cells, cartilage cells, smooth muscle cells, myocardial cells and nerve cells. Adipose-derived stem cells and mesenchymal stem cells have very similar biological properties, and they are also very close in terms of cell surface marker expression profile.
    OBJECTIVE: To investigate the expression of human leukocyte antigen II in adipose-derived stem cells and its immunogenicity and immunomodulatory effect, and to explore the specific mechanism.
    METHODS: After isolation and culture, the adipose-derived stem cells were stimulated with interferon-γ, and the expression of human leukocyte antigen II was detected by flow cytometry, the expression of indoleamine 2,3-dioxygenase was determined by real-time PCR and western blot. Adipose-derived stem cells were used to stimulate lymphocytes, and the cell proliferation was observed. The lymphocytes were stimulated with 
    phytohemagglutinin, and then 1×102-1×105 adipose-derived stem cells were added to inhibit the proliferation; meanwhile, adipose-derived stem cells, a third party, at a dose of 1×102-1×105 were added to the ongoing two-way mixed lymphocyte reaction, and the cell number was calculated as count per minute.
    RESULTS AND CONCLUSION: The expression of human leukocyte antigen II and indoleamine 2,3-dioxygenase was low in adipose-derived stem cells, but up-regulated with stimulation of interferon-γ. Adipose-derived stem cells could not stimulate lymphocyte proliferation, but could inhibit lymphocyte proliferation induced by phytohemagglutinin or allogeneic lymphocytes, and interferon-γ was highly expressed in the supernatant of co-culture of adipose-derived stem cells and mixed lymphocytes. These results indicate that the adipose-derived stem cells have low immunogenicity and can inhibit cell proliferation of lymphocytes in vitro, and the addition of exogenous interferon-γ can enhance the immunosuppressive effects of adipose-derived stem cells.


    中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程


    全文链接:

    Figures and Tables | References | Related Articles | Metrics
    Edaravone protects bone marrow stromal cells from oxidative injury
    Zhang Zhen-yu, Gao Ming, Feng Lei, Cao Yun-tao, Chen Lei, Li Ming-chao, Liu Chao
    2014, 18 (32):  5126-5131.  doi: 10.3969/j.issn.2095-4344.2014.32.008
    Abstract ( 226 )   PDF (2238KB) ( 396 )   Save

    BACKGROUND: Edaravone as an antioxidant protective effect on nerve cells injured by hydrogen peroxide has been confirmed, but its protective effect on oxidative damage to bone marrow stromal cells has not been reported in-depth.
    OBJECTIVE: To investigate the regulatory effects of edaravone on oxidative injury to bone marrow stromal cells.
    METHODS: Bone marrow samples were extracted from the long bone of New Zealand rabbits by the method of washing the pulp cavity, then subjected to the density gradient centrifugation and adherent screening to obtain bone marrow stromal stem cells in vitro. The bone marrow stromal cells at 3 passage were divided into five groups: blank group, treated with low-glucose Dulbecco’s modified Eagle’s medium containing 10% fetal bovine serum and 1% double antibody; dexamethasone group, treated with cell culture medium containing 1×10-7 mol/L dexamethasone; 50, 100, 300 mg/L edaravone groups, cultured in cell culture medium containing 1×10-7 mol/L dexamethasone and 50, 100, 300 mg/L edaravone, respectively. After culture, MTT method and flow cytometry were used to detect the proliferative level and cell cycle of cells.
    RESULTS AND CONCLUSION: Compared with the control and dexamethasone groups, edaravone significantly enhanced the cell proliferation. Edaravone played a protective role in bone marrow stromal cells. When the concentration was 50 mg/L, edaravone began to play a regulatory role (P < 0.05), and this effect was certainly associated with the concentration of edaravone. When the concentration was up to 100 mg/L, edaravone showed a better protective role
    (P < 0.01). However, with increasing concentration, this protective effect was not further increased, but decreased slightly. Results indicated that high-concentration dexamethasone can induce oxidative injury to bone marrow stromal cells, and edaravone can protect the cells against this oxidative damage by antioxidant role.


    中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程


    全文链接:

    Figures and Tables | References | Related Articles | Metrics
    Effects and mechanisms of human umbilical cord-derived mesenchymal stem cells on the thymus development in nude mice
    Wang Li-ming, Wang Li-hua, Li Ming, Wang Qian-yun, Yang Jie, Liu Guang-yang, Cong Xiu-li,Liu Yong-jun
    2014, 18 (32):  5132-5139.  doi: 10.3969/j.issn.2095-4344.2014.32.009
    Abstract ( 249 )   PDF (2898KB) ( 472 )   Save

    BACKGROUND: For autoimmune diseases, it is difficult to effectively solve the lack of immunological tolerance in patients by traditional treatments. Mesenchymal stem cells have the biological functions of tissue and organ regeneration and immune regulation.
    OBJECTIVE: To explore the effects and mechanisms of human umbilical cord-derived mesenchymal stem cells on the development of thymus in nude mice.
    METHODS: Human umbilical cord-derived mesenchymal stem cells were intraperitoneally injected into BABL/c nude mice at a dose of 2×106 per mouse. We analyzed the maturation and distribution of thymic epithelial cells in the thymus rudiment of nude mice and the thymopoiesis of this newly developed thymus rudiment; furthermore, we explored the therapeutic effects of human umbilical cord-derived mesenchymal stem cells.
    RESULTS AND CONCLUSION: Our data showed well-organized cortex-medulla structure and an obvious improvement in the maturation of thymic epithelial cells in the rudiment. We further demonstrated the improved thymopoiesis and the enhanced export of mature T cells with the T regulatory cells increase in peripheral blood. 
    Furthermore, we found that human umbilical cord-derived mesenchymal stem cells could be engrafted in the thymus and express many cytokines, especially keratinocyte growth factor which is essential for the thymus development. These findings indicate that a new mechanism of human umbilical cord-derived mesenchymal stem cells can provide a proper microenvironment for the reconstitution and functional maturation of thymus in nude mice, and elicit another insight in terms of therapeutic efficacy of human umbilical cord-derived mesenchymal stem cells in many autoimmune diseases.


    中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程


    全文链接:

    Figures and Tables | References | Related Articles | Metrics
    Safety and feasibility of autologous bone marrow mesenchymal stem cells in treating chronic allograft nephropathy
    Zhang Lei, Chen Zheng1, Xie Si-sheng, Ma Jun-jie, Fang Jia-li, Li Guang-hui, Xu Lu, Zhang Yi-rui, Guo Yu-he, Pan Guang-hui
    2014, 18 (32):  5140-5145.  doi: 10.3969/j.issn.2095-4344.2014.32.010
    Abstract ( 261 )   PDF (2761KB) ( 486 )   Save

    BACKGROUND: Chronic allograft nephropathy is a complication of kidney transplantation and most of patients will eventually develop transplant kidney dysfunction. Bone marrow mesenchymal stem cells as a low immunogenicity special cell population have been shown to have differentiation, transdifferentiation, paracrine and other basic functions, which have been successful used in other clinical areas. Based on this characteristic, bone marrow mesenchymal stem cells may play a therapeutic role in chronic allograft nephropathy.
    OBJECTIVE: To study the safety and feasibility of autologus bone marrow mesenchymal stem cells transplantation via renal artery infusion and subsequent intravenous infusion guided by the digital subtraction angiography in the treatment of chronic allograft nephropathy. 
    METHODS: Eleven patients with chronic allograft nephropathy who were confirmed from March 2011 to January 2013 were enrolled, and then received transplant renal artery infusion once guided by the digital subtraction angiography and subsequent intravenous infusion twice of bone marrow mesechymal stem cells. Changes in serum creatinine, blood urea nitrogen, creatinine clearance, cystatin C, 24-hour urine protein, and β2 microglobulin in the blood and urinary were monitored in patients up to 1 year after treatment.
    RESULTS AND CONCLUSION: Bleeding, transplant renal artery embolization, pseudoaneurysm and other related complications were not found in any of the 11 patients. The levels of serum creatinine, blood urea nitrogen and cystatin C were significantly decreased at 1 week and 1 month after cell therapy (P < 0.05), while after 3 months of treatment, there was no difference before and after treatment (P > 0.05). The creatinine clearance at 1 week and 1 month after treatment showed a remarkable increase, which were significantly different from that before treatment (P < 0.05), but after 3 months of treatment, the difference was not significant (P > 0.05). The level of 24-hour urine protein was significantly decreased after 7 days of treatment (P < 0.05), and no difference was found after 1 month (P > 0.05). The level of β2 microglobulin in the blood and urinary had no changes before and after treatment. These findings indicate that guided by the digital subtraction angiography, bone marrow mesenchymal stem cells via the renal artery infusion and subsequent intravenous infusion can improve kidney function of patients, but the cell dosage and infusion method remain to be solved.


    中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程


    全文链接:

    Figures and Tables | References | Related Articles | Metrics
    Endothelial progenitor cells repair ischemia-reperfusion injury in rats
    Zhou Li-na, Wang Yu-xin, Fang Lin, Wu Ting, Yu Yi
    2014, 18 (32):  5146-5151.  doi: 10.3969/j.issn.2095-4344.2014.32.011
    Abstract ( 355 )   PDF (902KB) ( 855 )   Save

    BACKGROUND: Endothelial progenitor cells are recruited into local vascular injury under the injury-induced stimulation, and then differentiate into mature endothelial cells that are thereby involved in angiogenesis and endothelial repair.
    OBJECTIVE: To investigate whether endothelial progenitor cells can alleviate renal injury and improve renal function of ischemia/reperfusion injury (I/R) rats.
    METHODS: Peripheral blood samples extracted from Sprague-Dawley rats were used to isolate and culture endothelial progenitor cells using density gradient centrifugation. Twenty-two male Sprague-Dawley rats were randomized to three groups: I/R group, normal control group, and endothelial progenitor cells group. In the I/R and endothelial progenitor cells groups, the right kidney was removed and the renal artery and vein of the left kidney were occluded for 40 minutes to establish I/R models in the rats, and then endothelial progenitor cells (5×109/L, totally 1 mL) or solvent was transplanted via the artery of the left kidney into the left kidney. In the normal control group, the experimental procedure was same as that in the I/R group except for occlusion of the  
    artery and vein of the left kidney. Renal and blood samples from three groups were collected at day 1 after operation. Peripheral blood CD34 and vascular endoethelial growth factor receptor 2 expressions were determined using flow cytometry and immunofluorescence methods, serum creatinine and urea nitrogen were tested, and immunohistochemistry observation was used for CD34 observation.
    RESULTS AND CONCLUSION: Compared with the normal control group, serum creatinine and urea nitrogen levels were significantly increased, and tubulointerstitial CD34 expression was decreased in the I/R group (P < 0.05). Endothelial progenitor cells treatment largely decreased the levels of serum creatinine and urea nitrogen, and increased CD34 expression (P < 0.05). These findings indicate that transplantation of endothelial progenitor cells contributes to renal protection in I/R rats.


    中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程


    全文链接:

    Figures and Tables | References | Related Articles | Metrics
    Changes in the activity of stem/progenitor cells in the rat submandibular gland following ligation of the main excretory duct
    Chen Wei, Huang Gui-Lin
    2014, 18 (32):  5152-5157.  doi: 10.3969/j.issn.2095-4344.2014.32.012
    Abstract ( 279 )   PDF (907KB) ( 450 )   Save

    BACKGROUND: Fewer ethical issues exist in adult stem cells, and some operating technologies are relatively mature. Therefore, to construct tissue-engineered salivary glands using adult stem cells is very attractive and seductive with an extremely important application prospect.
    OBJECTIVE: To establish a rat model of salivary gland injury by ligation of the main excretory duct of the submandibular gland and to explore the existing feasibility and location of adult stem cells in the injured models.
    METHODS: The main excretory duct of the right submandibular glands was ligated in Sprague-Dawley rats. After 1 week, rats were killed to remove the bilateral glands that were then subject to hematoxylin-eosin staining, PAS glycogen staining and immunohistochemical staining for determination of CK-19, Bcl-2, Ki-67. After that, we compared the normal submandibular gland with the damaged model after ligation of main excretory duct.
    RESULTS AND CONCLUSION: The rats showed differences in the volume and mass of the affected and normal submandibular glands. The normal submandibular gland was oval, ruddy, smooth, soft with an intact envelop. After ligation, the injured submandibular gland appeared to have atrophy with dark red in color, irregular morphology, envelop congestion, and rough texture; the surrounding vessels showed compensatory expansion. PAS-positive gland cells disappeared, CK-19-postive small duct epithelial cells proliferated, and laminin-positive cells that were rarely found in the normal gland existed around the duct. In addition, Bcl-2/Ki-67 positive cells 
    were both increased. These findings indicate that stem/progenitor cells may be located in the periductal area of the submandibular gland; and the model of submandibular gland injury established by ligation of the main excretory duct is effective to activate stem/progenitor cells in the submandibular gland.


    中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程


    全文链接:

    Figures and Tables | References | Related Articles | Metrics
    Bone marrow mesenchymal stem cell transplantation for treatment of diabetes: “transdifferantiation” appears? 
    Chen Chu-xian, Ren Li-li, Jiang Jin-xing, Zhang Jie-jie, Ye Li-zi, Li Fu-rong, Deng Chun-yan
    2014, 18 (32):  5158-5165.  doi: 10.3969/j.issn.2095-4344.2014.32.013
    Abstract ( 238 )   PDF (2679KB) ( 436 )   Save

    BACKGROUND: In recent years a large number of studies have suggested that bone marrow mesenchymal stem cells can ease hyperglycemia of diabetic rats, but the related mechanism is unclear and controversial.
    OBJECTIVE: To investigate the relevant mechanism of bone marrow mesenchymal stem cells on pancreas microenvironment in vivo in diabetic rats.
    METHODS: Bone marrow mesenchymal stem cells were transfected with enhanced green fluorescent protein (EGFP) and administered to diabetic rats via the subcapsular pancreas. Blood glucose levels were monitored. The expressions of the key genes in islet development in these EGFP positive pancreatic cells were analyzed by 
    Real-time quantitative PCR at different times. EGFP and insulin double-positive cells were detected by immunofluorescence. Flow cytometry was performed to analyze cell cycle and DNA ploidy.
    RESULTS AND CONCLUSION: Blood glucose levels were effectively reduced after transplantation. The expressions of the key genes in islet development reached their own peak values at different times after transplantation: Nestin at week 1, Nkx 2.2 at week 3, Pax 4 and Ngn 3 at week 4, insulin and glucagon at week 12, PDX-1 at week 8 until week 12. The cells double-positive for EGFP and insulin cells were observed. In the pancreas, EGFP positive cells at S+G2/M phase were significantly increased, and there were no polyploid and aneuploid cells. In pancreas microenvironment, the bone marrow mesenchymal stem cells transplanted into the diabetic pancreas can differentiate into islet β-like cells under gene control, but not through the fusion with tissue cells.


    中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程


    全文链接:

    Figures and Tables | References | Related Articles | Metrics
    Growth characteristics of umbilical cord-derived versus embryonic liver-derived mesenchymal stem cells
    Bi Wei-wei, He Li-rong, Nian Li-li
    2014, 18 (32):  5166-5172.  doi: 10.3969/j.issn.2095-4344.2014.32.014
    Abstract ( 346 )   PDF (1952KB) ( 732 )   Save

    BACKGROUND: Different sources of stem cells have different molecular characteristics and growth characteristics; therefore, there are some differences in therapeutic mechanisms and effects.
    OBJECTIVE: To compare mesenchymal stem cells growth characteristics form two sources.
    METHODS: Mesenchymal stem cells from the umbilical cord and the embryonic liver were isolated and cultured. Passage 5 cells were used to observe the cell morphology, calculate the doubling time of cell population-doubling time, identify surface markers and determine the differentiation capacity. Mesenchymal stem cells from the umbilical cord were subcultured to passages 10 and 15, and cell curves were drawn and population doubling time was calculated.
    RESULTS AND CONCLUSION: Mesenchymal stem cells from these two sources in logarithmic phase were fusiform and grew spirally with osteogenic, adipogenic, and chondrogenic capacities. The growth curves of cells were both S-shaped. At passage 5, the doubling time was (34.37±0.31) hours for embryonic liver-derived mesenchymal stem cells and (35.63±0.38) hours for umbilical cord-derived mesenchymal stem cells, and there was no significant difference (P > 0.05). However, the population doubling time of passages 10 and 15 umbilical cord-derived mesenchymal stem cells was (52.6±0.53) and (53.27±0.92) hours, respectively, which was significantly difference from that of passage 5 cells (P < 0.05). The cell morphology and growth curve from two sources are basically the same. Embryonic liver-derived stem cells are smaller and proliferate faster than umbilical cord-derived mesenchymal stem cells, but no statistical difference is found between the two types.


    中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程


    全文链接:

    Figures and Tables | Related Articles | Metrics
    Hearing restoration in a deaf animal model with transplantation of adipose-derived mesenchymal stem cells from guinea pigs
    Wang Xiao-yan, Ji Bin, Li Bing-bing, Bi Xiao-juan, Liu Li-zhong
    2014, 18 (32):  5173-5177.  doi: 10.3969/j.issn.2095-4344.2014.32.015
    Abstract ( 308 )   PDF (1783KB) ( 401 )   Save

    BACKGROUND: Can adipose-derived mesenchymal stem cells be used to treat sensorineural deafness caused by hair cell degeneration and loss?
    OBJECTIVE: To investigate the effect of adipose-derived mesenchymal stem cells from guinea pigs transplanted via the scala tympani on the recovery from sensorineural hearing in an animal model.
    METHODS: Intraperitoneal injection of gentamicin was performed to prepare sensorineural deafness models in guinea pigs. Then, adipose-derived mesenchymal stem cells from guinea pig were transplanted via the scala tympani. After 1 and 3 weeks, auditory brainstem responses were detected to observe the hearing variation after adipose-derived mesenchymal stem cell transplantation. Meanwhile, the migration and distribution of EDU-labeled adipose-derived mesenchymal stem cells in the cochlea were traced.
    RESULTS AND CONCLUSION: After 1 and 3 weeks of implantation, the results from auditory brainstem response showed that the hearing was gradually improved. At 1 week of implantation, most of the cells were distributed in the perilymph and some cells migrated to the Corti organ and attached to the basement membrane. At 3 weeks of implantation, cells migrated and attached to the basement membrane of Corti organ, furthermore, some cells migrated to the cochlear nerve. The longer the time of implantation, the less cell survived. The results indicate that adipose-derived mesenchymal stem cells from guinea pig that are transplanted via the scala tympani can directly migrate and survive ultimately to improve the hearing.


    中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程


    全文链接:

    Figures and Tables | References | Related Articles | Metrics
    Effect of Toll-like receptor 4 on the immunological properties of periodontal ligament stem cells
    Ding Gang, Wei Li-mei, Zhang Li, Tang Rui-ling
    2014, 18 (32):  5178-5183.  doi: 10.3969/j.issn.2095-4344.2014.32.016
    Abstract ( 378 )   PDF (728KB) ( 393 )   Save

    BACKGROUND: Toll-like receptor 4 (TLR4) and its ligand, lipopolysaccharid, are closely associated with the occurrence and development of periodontitis. Meanwhile, the immunological properties of periodontal ligament stem cells (PDLSCs) play an important role in the reconstruction of periodontal tissue and cell-based therapy of periodontitis. However, the effect of TLR4 and lipopolysaccharid on the immunological properties of PDLSCs remains unclear.
    OBJECTIVE: To investigate the effect of TLR4 on the immunological characteristics of human PDLSCs.
    METHODS: PDLSCs were isolated by enzyme digestion method as previously reported, and were cultured in the medium containing 10 mg/L lipopolysaccharid, the ligand of TLR4 for 3 days. Using un-treated PDLSCs as controls, we then investigated whether lipopolysaccharid-treated PDLSCs could cause the proliferation of 
    allogeneic T lymphocytes as well as the effect of lipopolysaccharid-treated PDLSCs on the mixed lymphocytes reaction and proliferation of lymphocytes induced by phytohemagglutinin. PDLSCs, peripheral blood mononuclear cells and phytohemagglutinin were co-cultured, and the concentration of prostaglandin E2 in the culture supernatant was examined. Then we added indomethacin, which is the inhibitor of prostaglandin E2, into the co-culture system of PDLSCs, peripheral blood mononuclear cells and phytohemagglutinin, and tested the proliferation of lymphocytes.
    RESULTS AND CONCLUSION: Lipopolysaccharid-treated PDLSCs did not lead to the proliferation of allogeneic T lymphocytes just as un-treated PDLSCs, and could suppress the mixed lymphocytes reaction and proliferation of phytohemagglutinin-induced lymphocytes. However, the inhibitory ability of lipopolysaccharid-treated PDLSCs was significantly lower than that of un-treated PDLSCs. The levels of prostaglandin E2 were significantly elevated in the co-culture of PDLSCs, peripheral blood mononuclear cells and phytohemagglutinin. After adding of indomethacin, the PDLSCs-suppressed proliferation of lymphocytes restored to normal levels. Lipopolysaccharid weakened the immunosuppressive capacity of PDLSCs, which may be due to the decreasing secretion of prostaglandin E2.


    中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程


    全文链接:

    Figures and Tables | References | Related Articles | Metrics
    Osthole promotes the proliferation of neural stem cells in vitro
    Yao Ying-jia, Hu Yu, Li Shao-heng, Jiao Ya-nan, Kong Liang, Tao Zhen-yu, Yang Jing-xian
    2014, 18 (32):  5184-5189.  doi: 10.3969/j.issn.2095-4344.2014.32.017
    Abstract ( 287 )   PDF (882KB) ( 534 )   Save

    BACKGROUND: Neural stem cells have self-renewal and multidirectional differentiation potential, but under normal circumstances, the number of neural stem cells is less, and most cells are in the resting state. Thus, to promote the proliferation of neural stem cells is the key to the treatment of neurodegenerative diseases.
    OBJECTIVE: To investigate the effects of osthole on the proliferation of neural stem cells cultured in vitro, and to analyze its mechanism underlying promoting the proliferation.
    METHODS: Neural stem cells were cultured in vitro, and passage 3 cells were cultured with different concentrations of osthole(10, 50 and 100 μmol/L). After 24 hours, cell vitality was determined by cell counting kit-8. After 3, 5, 7 days of further culture, the radius of neurospheres was measured, and Ki67-positive cells were counted by immunofluorescence staining. Meanwhile, after 3 days of further culture, the gene expression of Notch 1, Hes 1 and Mash 1 in neural stem cells was detected by RT-PCR.
    RESULTS AND CONCLUSION: Compared with the control group, 50, 100 μmol/L osthole could obviously promote the proliferation ability of neural stem cells. 100 μmol/L osthole had the most significant effect and increased the expression of Notch 1 gene, Hes 1 gene, but it had no effect on Mash 1 gene. These results suggest that osthole can promote proliferation of neural stem cells cultured in vitro and its mechanism may be associated with activation of Notch 1 gene and Hes 1 gene in Notch signaling pathway.


    中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程


    全文链接:

    Figures and Tables | References | Related Articles | Metrics
    Biological characteristics of endothelial progenitor cells derived from granulocyte colony-stimulating factor mobilized peripheral blood
    Bai Li-ping, Zhao Zhi-hong, Chen Chong, Wang Jin-huan, Wang Zhen-ling, Zhao Zhi-gang
    2014, 18 (32):  5190-5196.  doi: 10.3969/j.issn.2095-4344.2014.32.018
    Abstract ( 314 )   PDF (2027KB) ( 434 )   Save

    BACKGROUND: Endothelial progenitor cells have good prospects for clinical application; especially as seed cells, they are involved in construction of tissue engineered blood vessels and disease treatment.
    OBJECTIVE: To investigate the biological characteristics of endothelial progenitor cells derived from granulocyte colony-stimulating factor (G-CSF) mobilized peripheral blood.
    METHODS: Mononuclear cells from G-CSF mobilized peripheral blood (n=9) and normal adult peripheral blood (n=8) were obtained and cultured in expanded medium. The immunophenotype of endothelial progenitor cells was investigated by FACS and immunohistochemistry. Real-time PCR was used to detect the expression of different cytokines. Proliferation and adhesion ability of endothelial progenitor cells were detected by MTT assay and adhesion assay. Moreover, the abilities of vasculogenesis in vitro and Dil-labeled acetylated low density  lipoprotein uptake were detected. The acquired cells were seeded onto the basilar membrane gel containing vascular endothelial growth factor and basic fibroblast growth factor to induce angiogenesis.
    RESULTS AND CONCLUSION: Endothelial progenitor cells derived from G-CSF mobilized peripheral blood were similar to those from normal adult peripheral blood in phenotype and morphology. FACSs and immunohistochemistry showed that endothelial progenitor cells were positive for endothelial cell markers, such as CD31, vWF, CD34, FLK-1, VE-Cadherin and CD133. In addition, the expression of vascular endothelial growth factor and stromal cell-derived factor 1 were significantly increased in G-CSF mobilized endothelial progenitor cells. Moreover, endothelial progenitor cells derived from two sources possessed the abilities of angiogenesis in vitro and Dil-labeled acetylated low density lipoprotein uptake. But, the number and the proliferation ability of endothelial progenitor cells derived from G-CSF mobilized peripheral blood were better than that of endothelial progenitor cells derived from normal adult peripheral blood. These findings indicate that there is a population of cells with characteristics of endothelial progenitor cells in G-CSF mobilized peripheral blood. They possess the abilities of vasculogenesis in vitro. Moreover, compared to endothelial progenitor cells derived from normal adult peripheral blood, we could obtain more endothelial progenitor cells in G-CSF mobilized peripheral blood and those cells show better proliferation ability.


    中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程


    全文链接:

    Figures and Tables | References | Related Articles | Metrics
    Lentiviral vector-mediated transfection of bone morphogenetic protein 2 gene into endothelial progenitor cells from rat bone marrow
    Yin Xiu-ru, Pei Ling, Liang Zuo-di
    2014, 18 (32):  5197-5202.  doi: 10.3969/j.issn.2095-4344.2014.32.019
    Abstract ( 332 )   PDF (2132KB) ( 348 )   Save

    BACKGROUND: Gene therapy has become a new trend for disease therapy and brought promise for some refractory diseases. The key point is to choose the proper cell, gene and vector.
    OBJECTIVE: To investigate the effectiveness and feasibility of bone morphogenetic protein 2 (BMP2) gene transfected into endothelial progenitor cells (EPCs) from rat bone marrow for gene therapy.
    METHODS: The EPCs were isolated, cultured and identified from the bone marrow of Sprague-Dawley rats. Empty vector (LV-eGFP) or BMP2 gene (LV-eGFP-BMP2) was transferred into EPCs by the constructed lentiviral vector (LV). We examined the transfection efficiency by eGFP fluorescence, BMP2 secretion by ELISA, BMP2 expression by Western blot, and compared the capacities of migration, proliferation and anti-apoptosis after transfection in the three groups of normal EPCs, empty vector-EPCs, and BMP2-EPCs.
    RESULTS AND CONCLUSION: The transfection efficiency of lentiviral vector was 90%. BMP2 gene-transferred EPCs secreted and expressed more BMP2 proteins (P < 0.01), and showed enhanced anti-apoptotic ability (P < 0.05). The proliferation and migration capacity did not change obviously (P > 0.05). After successful transfection with lentivirus-BMP2 gene, EPCs can secrete and express more BMP2 protein and show enhanced anti-apoptotic ability without obvious influence on the proliferation and migration capacity.


    中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程


    全文链接:

    Figures and Tables | References | Related Articles | Metrics
    Chondrogenic differentiation of placenta-derived mesenchymal stem cells in vitro
    Li Zhi, Zhao Wei, Liu Wei, Zhou Ye, Jia Jing-qiao, Yang Li-feng
    2014, 18 (32):  5203-5208.  doi: 10.3969/j.issn.2095-4344.2014.32.020
    Abstract ( 324 )   PDF (1845KB) ( 485 )   Save

    BACKGROUND: Placenta-derived mesenchymal stem cells have been shown to have a strong proliferative capacity, and can differentiate into osteoblasts, neuroblasts, hepatocyte-like cells, but there are few studies about their chondrogenic differentiation.
    OBJECTIVE: To induce the differentiation of human placenta-derived mesenchymal stem cells into chondrocytes in vitro.
    METHODS: Human placenta-derived mesenchymal stem cells were culture in vitro and identified. Passage 3 cells were used to prepare cell suspensions at the concentration of 1.6×1010 cells/L, and then cultured in 24-well plates at a volume of 5, 10, 15 μL for 2 hours for the purpose of forming micelles. After that, the samples were cultured in mesenchymal stem cell medium or cartilage induction medium for 14 days. Alcian blue staining, general observation and inverted microscope observation were performed.
    RESULTS AND CONCLUSION: A great amount of adherent cells formed in the mesenchymal stem cell medium, which possessed typical morphology of mesenchymal stem cells. After cultured in cartilage induction medium, the cells only maintained micelles and did not proliferate continuously to form adherent cells. The base of these micelles had no adherent cells, and the diameter of micelles became increasing with the increasing number of inoculated cells. These findings suggest that human placenta-derived mesenchymal stem cells have the chondrogenic ability, and can be used as seed cells for tissue engineering and cell therapy.


    中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程


    全文链接:

    Figures and Tables | References | Related Articles | Metrics
    Construction and expression of NDRG2 retroviral vector
    Ou Jian-feng, Ren Hui, Wang Cun-bang, Bai Hai
    2014, 18 (32):  5209-5213.  doi: 10.3969/j.issn.2095-4344.2014.32.021
    Abstract ( 229 )   PDF (576KB) ( 637 )   Save

    BACKGROUND: N-Myc downstream regulated gene 2 (NDRG2) is a new tumor supressor gene, and it can either improve anti-tumor effect by classic pathway or monitor carcinogenesis. But there is yet no report addressing NDRG2 role in myeloma occurrence.
    OBJECTIVE: Using pBaba-puro retroviral vector to construct recombinant vector which carrying NDRG2 gene and pack virus, then to screen U266 cells stably expressing NDRG2.
    METHODS: Primers were designed and synthesized. RNA was extracted from U266 cells, and then applied to reverse transcription for the template and latter PCR amplification. After PCR amplification and digested by Taq I and BamHI, agarose gel electrophoresis was used to detect the correct fragments. We used the vectors to package the retrovirus and infected the U266 cells. The U266 cells stably expressing NDRG2 were screened and NDRG2 protein was detected by western blot assay.
    RESULTS AND CONCLUSION: The vector carrying NDRG2 was successfully constructed. The retrovirus was also packaged and infected U266 cells. The U266 cells stably expressing NDRG2 were screened. In these U266 cells, NDRG2 expression was increased significantly. The use of recombinant retrovirus vector technology can successfully construct a retrovirus carrying the corresponding gene which can be used for the following study on NDRG2 function in human myeloma.


    中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程


    全文链接:

    Figures and Tables | Related Articles | Metrics
    Endothelial progenitor cells for diabetic vascular complications: is it a new therapeutic strategy?
    Gao Cai-yan, Mai Chao-ping, Ha Xiao-qin
    2014, 18 (32):  5214-5219.  doi: 10.3969/j.issn.2095-4344.2014.32.022
    Abstract ( 293 )   PDF (630KB) ( 544 )   Save

    BACKGROUND: Because of the high potential of differentiation and involvement in endothelial repair and angiogenesis, endothelial progenitor cells have broad prospects in the treatment of diabetic vascular complications.
    OBJECTIVE: To review the changes of endothelial progenitor cells in patients with diabetic vascular complications and the therapeutic application of endothelial progenitor cells in diabetic vascular diseases.
    METHODS: We searched PubMed database and CNKI database with the key words of “endothelial progenitor cells, diabetes mellitus, diabetic vascular complications” in English and Chinese, respectively, for relevant articles published from January 1997 to May 2014. A total of 147 English literatures and 26 Chinese literatures were searched, and finally 44 papers were involved in the analysis.
    RESULTS AND CONCLUSION: Because of increased oxidative stress, impairment of the antioxidant system, reduction of nitric oxide synthesis, and the loss of response to hypoxia, the number and mobilization of endothelial progenitor cells in diabetic patients are reduced, and their capacity of adhesion, migration, and angiogenesis are impaired, which lead to diabetic vascular complications. Transplantation of healthy endothelial progenitor cells can improve the number and function of endothelial progenitor cells, and promote the mobilization and chemotaxis of endothelial progenitor cells to ischemic tissue in patients with diabetic vascular diseases, which has become the new therapeutic strategy for diabetic vascular complications.


    中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程


    全文链接:

    Figures and Tables | References | Related Articles | Metrics
    The preclinical and clinical application of genetically modified stem cells for the treatment of myocardial infarction
    Gao Qing, Li Shu-ren
    2014, 18 (32):  5220-5224.  doi: 10.3969/j.issn.2095-4344.2014.32.023
    Abstract ( 238 )   PDF (569KB) ( 439 )   Save

    BACKGROUND: Stem cells possess the potential of differentiation into myocardium and vessels, which can be transplanted into myocardial ischemia parts for tissue repair and revascularization. Therefore, stem cell transplantation has emerged as a promising alternative therapeutic approach for the treatment of ischemic heart disease. However, the long-term survival and curative efficacy issue of stem cells remains a problem. The emergence of genetic modification in combination with stem cell transplantation for stem cell research provides a new way of thinking.
    OBJECTIVE: To explore stem cell types for cardiac regeneration therapy, the selection of the therapeutic gene, the genes vectors and transplantation way and to overview the clinical application of gene-modified stem cells in the field of cardiovascular disease.
    METHODS: A computer-based search of PubMed (2006-01/2013-12) was performed to collect articles about stem cells. The key words included “stem cells; genetic therapy; myocardial infarction; regenerative medicine; tissue construction”. Articles related to stem cells published recently or in high-impact journals were initially surveyed and finally 40 articles were included and summarized.
    RESULTS AND CONCLUSION: The efficacy of stem cell transplantation may be significantly augmented by genetic modification. Bone marrow mesenchymal stem cells are one of the most widely used seed cells currently. The anti-apoptosis, pro-angiogenic, anti-inflammatory genes mediated by the adeno-associated virus and adenovirus were transfected into stem cells, so that the curative effect of stem cell transplantation will be significantly increased. Genetically modified stem cell transplantation has the potential application in a variety of clinical diseases, including myocardial infarction, but its long-term safety remains to be further studied.


    中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程


    全文链接:

    Figures and Tables | References | Related Articles | Metrics
    Stem cell transplantation in the treatment of diabetic lower extremity vascular disease: cytokines and angiogenesis
    Zhong Yong-hong
    2014, 18 (32):  5225-5229.  doi: 10.3969/j.issn.2095-4344.2014.32.024
    Abstract ( 310 )   PDF (682KB) ( 708 )   Save

    BACKGROUND: Stem cells under the certain condition can be induced to differentiate into vascular endothelial cells, to promote local angiogenesis and generate new capillary network and establish abundant collateral circulation, in order to achieve the purpose of improvement and treatment of lower limb ischemia.
    OBJECTIVE: To review the pathogenesis of diabetic lower extremity vascular lesions, and the basic and clinical development of autologous peripheral blood stem cell transplantation, umbilical cord blood stem cell transplantation, and stem cell transplantation combined with cytokines for the treatment of diabetic lower extremity vascular lesions.
    METHODS: A computer-based online search was performed in the PubMed database for relevant literatures published from 1983 to 2014. The keywords were “stem cells; stem cell transplantation; cord blood stem cell transplantation; diabetic angiopathies”.
    RESULTS AND CONCLUSION: The pathogenesis of diabetic lower extremity vascular lesions may be associated with dysimmunity-induced inflammation. It is lack of effective treatment methods. Stem cell transplantation in animal models and clinical treatment of diabetes complications has obtained certain achievements. Embryonic stem cells, mesenchymal stem cells and endothelial progenitor cells are widely used, and the their mechanism underlying treatment of diabetic lower extremity vascular lesions is mainly related to their participation in new vessel formation, starting and secreting factors, immune regulation, and stem cell proliferation and differentiation ability. Currently, the basic research is mainly limited to simple stem cell transplantation and stem cell transplantation combined with gene therapy. Compared with peripheral blood stem cell transplantation, autologous bone marrow stem cell transplantation is preferred because of more primitive cells, simpler operation, and lower cost. Studies have confirmed that cytokines secreted from stem cells are not enough to promote angiogenesis, but stimulate the skeletal muscle cells to secrete enough factors for promoting angiogenesis. The current clinical application of stem cells has a lot of problems to be solved.


    中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程


    全文链接:

    Figures and Tables | References | Related Articles | Metrics
    Mesenchymal stem cells therapy assists liver transplantation: aspects and prospects
    Xiang Jun-xi, Zheng Xing-long, Dong Ding-hui, Kang Xiao-hong, Li Jian-hui, Lv Yi
    2014, 18 (32):  5230-5236. 
    Abstract ( 278 )   PDF (691KB) ( 399 )   Save

    BACKGROUND: Marginal donor liver transplantation, auxiliary liver transplantation and partial liver transplantation that are developed due to the shortage of liver donors are still associated with an elevated risk of postoperative liver failure and increased mortality. In addition, postoperative immune related complications have become the bottleneck of restricting the development of liver transplantation. Mesenchymal stem cells based therapy is a potential innovation to solve these problems for its strong ability of regeneration, multi-lineage differentiation and immune regulation.
    OBJECTIVE: To summarize and discuss the aspects and prospects of mesenchymal stem cells therapy assisting liver transplantation.
    METHODS: Literature search was performed in PubMed, ScienceDirect, OvidSP, CNKI databases for relevant literatures published from 2000 to 2014. The key words are “mesenchymal stem cells, mesenchymal stromal cells, liver transplantation, immunosuppression, liver regeneration” in Chinese and English. Then, 74 papers were further analyzed and reviewed in line with the theme.
    RESULTS AND CONCLUSION: Mesenchymal stem cells have become a hotspot in cell therapy research due to their wide range of sources, strong regeneration and differentiation potential, and immunomodulatory effects. Mesenchymal stem cells have been tried to extend the lifespan of transplanted liver, to decrease the immunosuppressor use, and to improve the outcomes of recipients. Abundant experimental studies, preclinical researches and clinical trials have demonstrated the promising application of mesenchymal stem cells in liver transplantation. However, there are still many problems to be further studied in order to ensure the safety and effectiveness of mesenchymal stem cells before widely used in the patients.

    Figures and Tables | Related Articles | Metrics
    Parental peripheral blood haploidentical hematopoietic stem cell transplantation in treatment of children with relapsed and refractory acute leukemia
    Wan Ding-ming, He Hai-yan, Bian Zhi-lei, Xie Xin-sheng, Sun Ling, Sun Hui, Cao Wei-jie,Xuan Zhong-qian, Liu Fei
    2014, 18 (32):  5237-5243.  doi: 10.3969/j.issn.2095-4344.2014.32.026
    Abstract ( 343 )   PDF (865KB) ( 647 )   Save

    BACKGROUND: Children with relapsed and refractory acute leukemia have a very poor clinical effect by simple chemotherapy, and allogeneic hematopoietic stem cell transplantation is the only effective approach to cure the disease. Haploidentical hematopoietic stem cell transplantation is becoming gradually mature, and the data show it has gotten better clinical efficacy than identical sibling and unrelated matched hematopoietic stem cell transplantation. In addition, parents have high compliance in order to save the treatment interval, which ensures the stem cell number and prevents leukemia recurrence, significantly improving the transplantation success rate and long-term leukemia-free survival rate.
    OBJECTIVE: To retrospectively analyze the clinical efficacy of parental peripheral blood haploidentical stem cell transplantation in treating children with relapsed and refractory acute leukemia.
    METHODS: Thirty-five children with relapsed and refractory acute leukemia undergoing parental peripheral blood haploidentical stem cell transplantation were enrolled. “Modified 1,4-butanediol dimethanesulfonate/ cyclophosphamide+thymocyte immunoglobulin” conditioning regimen and triple therapy of methotrexate, cyclosporine A and mycophenolate mofetil were applied to prevent graft-versus-host disease.
    RESULTS AND CONCLUSION: All the 35 patients achieved full engraftment. (1) The median mononuclear cells was 5.82(3.23-8.45)×108/kg, the median CD34+ cells was 4.52 (2.37-11.51)×106/kg. (2) Within 100 days after stem cell infusion, the transplant related mortality was 14.3%. (3) Incidence of I-II degrees of acute graft-versus-host disease was 34.3%, III-IV incidence was 37.1%, and the total incidence of chronic graft-versus-host disease was 42.9%. (4) The 2-year leukemia-free survival rate was 42.9%; the 2-year overall survival rate was 51.4%; the leukemia relapse rate was 34.3%; median survival time was 24 months. Results show that if there are no human leukocyte antigen-identical sibling and unrelated human leukocyte antigen-matched donors, parental haploidentical peripheral blood stem cell transplantation is an effective and feasible treatment for children with relapsed and refractory acute leukemia.


    中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程


    全文链接:

    Figures and Tables | References | Related Articles | Metrics
    Second haploidentical hematopoietic stem cell transplantation for aplastic anemia complicated by lymphoma in one case undergoing a failed first transplantation
    Yan Hong-min, Wang Zhi-dong, Zheng Xiao-li, Zhu Ling, Han Dong-mei, Ding Li, Dong Lei, Liu Jing, Xue Mei, Wang Heng-xiang
    2014, 18 (32):  5244-5248.  doi: 10.3969/j.issn.2095-4344.2014.32.027
    Abstract ( 633 )   PDF (523KB) ( 594 )   Save

    BACKGROUND: It is fatal for children with acute severe aplastic anemia undergoing failed first allogeneic. If there are secondary lymphoma and other complications, treatment is more difficult. Up to now, there is yet no feasible treatment.
    OBJECTIVE: To investigate the possibility and safety of second haploidentical hematopoietic stem cell transplantation because of the failure in the first unrelated blood transplantation and secondary lymphoma.
    METHODS: A 3-year-old boy with severe aplastic anemia underwent HLA 8/10 matched unrelated peripheral blood stem cell transplantation on November 25, 2011. There was a major ABO mismatch. The time of neutrophil exceeding 0.5×109/L and platelets recovery exceeding 20×109/L were 11 and 14 days, respectively after transplantation. Bone marrow aspiration showed normal while full donor engraftment was found by chromosomal analyses on day 30. Grade I graft-versus-host disease occurred after 35 days. Autoimmune hemolytic anemia and pure red cell aplasia were also diagnosed on day +54. He was given high-dose gamma globulin, erythropoietin and glucocorticoids, and autoimmune hemolytic anemia and pure red cell aplasia were controlled. Unfortunately, on day +90, the boy suffered from fever and superficial lymph nodes of the bilateral neck. B ultrasound-guided needle biopsy was done on the right cervical lymph nodes, and post transplant lymphoproliferative disorder was considered. Pathological examination showed diffuse large B-cell lymphoma. Managements for patients consisted of withdrawal of immunosuppressive treatment and application of rituximab plus chemotherapy. After treatment, the lymph node shrank, Epstein-Barr virus copy number was decreased, and the body temperature recovered. However, on day +150, blood routine examination and bone marrow aspiration showed transplant failure. Then, the boy received the second haploidentical stem cell transplantation on May 15, 2012. The donor was his father. A myloablative condition regimen was selected: high-dose fludarabine+cyclosphosphamide+ busulfan+mitoxantrone+anti-CD52 monoclonal antibody. Hematopoietic stem cells and mesenchymal stem cells were re-infused simultaneously. Prophylaxis strategy for graft-versus-host disease consisted of cyclosporine A +short-term methotrexate+CD25 monoclonal antibody combined with mycophenolate mofetil. Nucleated cells and CD34+ cells were re-infused at a dose of 13.52×108/kg and 2.45×106/kg, respectively. The follow-up time was 24 months after transplantation.
    RESULTS AND CONCLUSION: After second implantation, the time of neutrophil exceeding 0.5×109/L and platelets recovery exceeding 20×109/L was 14 and 30 days, respectively. DNA test showed full donor engraftment was found. The swollen lymph nodes shrank after pretreatment, but tended to be enlarged at 2 months after surgery. After withdrawal of immunosuppressor and local radiotherapy, the lymph node shrank stably. PET showed no obvious area with presence of metabolic abnormalities. Regular follow-up every 6 months after transplantation has showed that the body has normal life and goes to school. Results suggest that the co-transplantation of haploidentical hematopoietic stem cells and mesenchymal stem cells is safe and effective for treatment of severe aplastic anemia following the failure of first transplantation. Pretreatments can faster hematopoietic recovery and control graft-versus-host disease, which is worth further clinical research.


    中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程


    全文链接:

    Figures and Tables | Related Articles | Metrics