Bone marrow mesenchymal stem cell transplantation for treatment of diabetes: “transdifferantiation” appears?

doi:10.3969/j.issn.2095-4344.2014.32.013

Abstract

Abstract:

BACKGROUND: In recent years a large number of studies have suggested that bone marrow mesenchymal stem cells can ease hyperglycemia of diabetic rats, but the related mechanism is unclear and controversial.
OBJECTIVE: To investigate the relevant mechanism of bone marrow mesenchymal stem cells on pancreas microenvironment in vivo in diabetic rats.
METHODS: Bone marrow mesenchymal stem cells were transfected with enhanced green fluorescent protein (EGFP) and administered to diabetic rats via the subcapsular pancreas. Blood glucose levels were monitored. The expressions of the key genes in islet development in these EGFP positive pancreatic cells were analyzed by
Real-time quantitative PCR at different times. EGFP and insulin double-positive cells were detected by immunofluorescence. Flow cytometry was performed to analyze cell cycle and DNA ploidy.
RESULTS AND CONCLUSION: Blood glucose levels were effectively reduced after transplantation. The expressions of the key genes in islet development reached their own peak values at different times after transplantation: Nestin at week 1, Nkx 2.2 at week 3, Pax 4 and Ngn 3 at week 4, insulin and glucagon at week 12, PDX-1 at week 8 until week 12. The cells double-positive for EGFP and insulin cells were observed. In the pancreas, EGFP positive cells at S+G2/M phase were significantly increased, and there were no polyploid and aneuploid cells. In pancreas microenvironment, the bone marrow mesenchymal stem cells transplanted into the diabetic pancreas can differentiate into islet β-like cells under gene control, but not through the fusion with tissue cells.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: bone marrow, mesenchymal stem cell transplantation, diabetes mellitus, pancreas, cell transdifferentiation

CLC Number:

R394.2

Chen Chu-xian, Ren Li-li, Jiang Jin-xing, Zhang Jie-jie, Ye Li-zi, Li Fu-rong, Deng Chun-yan. Bone marrow mesenchymal stem cell transplantation for treatment of diabetes: “transdifferantiation” appears? [J]. Chinese Journal of Tissue Engineering Research, 2014, 18(32): 5158-5165.

Figures/Tables 4

References

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