Mangiferin protects bone marrow mesenchymal stem cells against hypoxia

doi:10.3969/j.issn.2095-4344.2014.32.002

Abstract

Abstract:

BACKGROUND: Bone marrow mesenchymal stem cells have particularly applied prospects in tissue engineering. However, the death of transplanted cells limits the tissue regeneration. To search a new drug of anti-free radicals and protecting bone marrow mesenchymal stem cells is of great significance.
OBJECTIVE: To investigate the protective effects of mangiferin on bone marrow mesenchymal stem cells against hypoxia.
METHODS: Rat bone marrow mesenchymal stem cells were cultured in vitro and hypoxia cell model was established by cobalt chloride. Cells were divided into normal control group, hypoxia group (treated with cobalt chloride), and mangiferin groups (cobalt chloride+20, 40, 80, 160 µmol/L mangiferin). After 12 and 24 hours of hypoxia, superoxide dismutase, malondialdehyde, and catalase levels in the cell supernatant were determined. After 3, 6, 12, 24 hours of hypoxia, reactive oxygen species change was detected in each group.
RESULTS AND CONCLUSION: Mangiferin significantly improved the survival rate of bone marrow mesenchymal stem cells exposed to hypoxia, increased the intracellular superoxide dismutase and catalase activities, decreased intracellular malondialdehyde and reactive oxygen species levels, thereby effectively protecting bone marrow mesenchymal stem cells against hypoxia. These findings indicate that mangiferin has effective protection against hypoxia and strong antioxidant ability, and can significantly reduce oxidative damage.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: bone marrow, mesenchymal stem cells, anoxia, mangifera

CLC Number:

R394.2

Cheng Jian-wen, Zhao Jin-min, Li Xiao-feng, Tan Zhen. Mangiferin protects bone marrow mesenchymal stem cells against hypoxia[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(32): 5091-5096.

Figures/Tables 1

2.1 氯化钴对大鼠骨髓间充质干细胞缺氧损伤的细胞毒性实验具体结果参见作者前期研究[11]。 2.2　缺氧损伤12，24 h各组骨髓间充质干细胞培养上清液中超氧化物歧化酶活性　应用氯化钴建立细胞缺氧模型，以芒果苷进行预保护，测定大鼠骨髓间充质干细胞缺氧损伤12 h及24 h的总超氧化物歧化酶和CuZn-超氧化物歧化酶活性，观察芒果苷对大鼠骨髓间充质干细胞缺氧损伤的保护作用。结果可见，芒果苷对大鼠骨髓间充质干细胞缺氧损伤的超氧化物歧化酶作用呈时间依赖性及浓度依赖性。用200 μmol/L氯化钴处理细胞12，24 h，大鼠骨髓间充质干细胞总超氧化物歧化酶活性下降。20，40，80 μmol/L芒果苷组总超氧化物歧化酶活性明显高于200 µmol/L氯化钴组(P < 0.01)，随着芒果苷浓度的升高，芒果苷对大鼠骨髓间充质干细胞的保护作用逐渐增强。但是芒果苷浓度为160 μmol/L时总超氧化物歧化酶活性有所降低，但仍高于200 µmol/L氯化钴组(P < 0.05)，见表1。用200 μmol/L氯化钴处理细胞12，24 h，大鼠骨髓间充质干细胞CuZn-超氧化物歧化酶活性下降。 20 μmol/L芒果苷组CuZn-超氧化物歧化酶活性高于 200 µmol/L氯化钴组(P < 0.05)，随着芒果苷浓度的升高，芒果苷对大鼠骨髓间充质干细胞的保护作用逐渐增强。40，80，160 μmol/L芒果苷组CuZn-超氧化物歧化酶活性与200 µmol/L氯化钴组比较，差异有非常显著性意义(P < 0.01)，见表1。 2.3 各组骨髓间充质干细胞培养上清液中微量丙二醛水平应用氯化钴建立细胞缺氧模型，以芒果苷进行预保护，测定大鼠骨髓间充质干细胞缺氧损伤12 h及24 h的微量丙二醛水平，观察芒果苷对大鼠骨髓间充质干细胞缺氧损伤的保护作用。可见，芒果苷对大鼠骨髓间充质干细胞缺氧损伤的微量丙二醛作用呈时间依赖性及浓度依赖性。用200 μmol/L氯化钴处理细胞12，24 h，大鼠骨髓间充质干细胞微量丙二醛水平上升。20 μmol/L芒果苷组丙二醛水平低于200 µmol/L氯化钴组(P < 0.05)，随着芒果苷浓度的升高，芒果苷对大鼠骨髓间充质干细胞的保护作用逐渐增强。40，80，160 μmol/L芒果苷组丙二醛水平与 200 µmol/L氯化钴组比较，差异有非常显著性意义(P < 0.01)，见表2。 2.4 各组骨髓间充质干细胞培养上清液中过氧化氢酶活性应用氯化钴建立细胞缺氧模型，以芒果苷进行预保护，测定大鼠骨髓间充质干细胞缺氧损伤12 h及24 h的过氧化氢酶活性，观察芒果苷对大鼠骨髓间充质干细胞缺氧损伤的保护作用。可见，芒果苷对大鼠骨髓间充质干细胞缺氧损伤的过氧化氢酶作用呈时间依赖性及浓度依赖性。用200 μmol/L氯化钴处理细胞12 h及24 h，大鼠骨髓间充质干细胞过氧化氢酶活性下降。处理12 h时20 μmol/L芒果苷组过氧化氢酶活性与200 μmol/L CoCl2组比较未见明显变化(P > 0.05)，随着芒果苷浓度的升高，芒果苷对大鼠骨髓间充质干细胞的保护作用逐渐增强，40 μmol/L芒果苷组过氧化氢酶活性高于200 µmol/L氯化钴组(P < 0.05)，80，160 μmol/L芒果苷组过氧化氢酶活性明显高于 200 µmol/L氯化钴组(P < 0.01)。处理24 h时20 μmol/L芒果苷组过氧化氢酶活性高于200 μmol/L 氯化钴组(P < 0.05)；随着芒果苷浓度的升高，芒果苷对大鼠骨髓间充质干细胞的保护作用逐渐增强，40，80，160 μmol/L芒果苷组丙二醛水平与200 µmol/L氯化钴组比较，差异有非常显著性意义(P < 0.01)，见表3。 2.5 各组骨髓间充质干细胞内活性氧变化应用氯化钴建立细胞缺氧模型，以芒果苷进行预保护，测定大鼠骨髓间充质干细胞缺氧损伤3，6，12，24 h的细胞内活性氧变化，观察芒果苷对大鼠骨髓间充质干细胞缺氧损伤的保护作用。可见，芒果苷对大鼠骨髓间充质干细胞缺氧损伤的活性氧作用呈时间依赖性及浓度依赖性。用 200 μmol/L氯化钴处理细胞3，6，12，24 h，大鼠骨髓间充质干细胞活性氧上升，第3小时为最高值，随着时间的延长，活性氧水平逐渐下降。20 μmol/L芒果苷组活性氧水平低于200 µmol/L氯化钴组(P < 0.05)，随着芒果苷浓度的升高，芒果苷对大鼠骨髓间充质干细胞的保护作用逐渐增强。40，80，160 μmol/L芒果苷组活性氧水平与 200 µmol/L氯化钴组比较，差异有非常显著性意义(P < 0.01)，见表4。"

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