Chinese Journal of Tissue Engineering Research ›› 2014, Vol. 18 ›› Issue (32): 5091-5096.doi: 10.3969/j.issn.2095-4344.2014.32.002
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Cheng Jian-wen, Zhao Jin-min, Li Xiao-feng, Tan Zhen
Received:
2014-07-08
Online:
2014-08-06
Published:
2014-09-18
Contact:
Zhao Jin-min, M.D., Professor, Department of Traumatic Orthopaedics and Hand Surgery, First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China
About author:
Cheng Jian-wen, Studying for doctorate, Attending physician, Department of Traumatic Orthopaedics and Hand Surgery, First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China
Supported by:
the Special Scientific Fund for Traditional Chinese Medicine in Guangxi Health Bureau, No. GZKZ 10-011, GZKZ-Z1104; the Research Fund for the Doctoral Program of Higher Education, No. 20104503110002; Key Technology Research and Development Projects of Population Health and Food Safety of Guangxi Science and Technology Bureau, No. guikegong 1140003A-31; Self-Funded Research Projects of Guangxi Health Bureau, No. guiweiZ2012058, guiweiZ2012061; Projects of Guangxi Education Bureau, No. guijiao201010LX050
CLC Number:
Cheng Jian-wen, Zhao Jin-min, Li Xiao-feng, Tan Zhen. Mangiferin protects bone marrow mesenchymal stem cells against hypoxia[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(32): 5091-5096.
2.1 氯化钴对大鼠骨髓间充质干细胞缺氧损伤的细胞毒性实验 具体结果参见作者前期研究[11]。 2.2 缺氧损伤12,24 h各组骨髓间充质干细胞培养上清液中超氧化物歧化酶活性 应用氯化钴建立细胞缺氧模型,以芒果苷进行预保护,测定大鼠骨髓间充质干细胞缺氧损伤12 h及24 h的总超氧化物歧化酶和CuZn-超氧化物歧化酶活性,观察芒果苷对大鼠骨髓间充质干细胞缺氧损伤的保护作用。结果可见,芒果苷对大鼠骨髓间充质干细胞缺氧损伤的超氧化物歧化酶作用呈时间依赖性及浓度依赖性。用200 μmol/L氯化钴处理细胞12,24 h,大鼠骨髓间充质干细胞总超氧化物歧化酶活性下降。20,40,80 μmol/L芒果苷组总超氧化物歧化酶活性明显高于200 µmol/L氯化钴组(P < 0.01),随着芒果苷浓度的升高,芒果苷对大鼠骨髓间充质干细胞的保护作用逐渐增强。但是芒果苷浓度为160 μmol/L时总超氧化物歧化酶活性有所降低,但仍高于200 µmol/L氯化钴组(P < 0.05),见表1。 用200 μmol/L氯化钴处理细胞12,24 h,大鼠骨髓间充质干细胞CuZn-超氧化物歧化酶活性下降。 20 μmol/L芒果苷组CuZn-超氧化物歧化酶活性高于 200 µmol/L氯化钴组(P < 0.05),随着芒果苷浓度的升高,芒果苷对大鼠骨髓间充质干细胞的保护作用逐渐增强。40,80,160 μmol/L芒果苷组CuZn-超氧化物歧化酶活性与200 µmol/L氯化钴组比较,差异有非常显著性意义(P < 0.01),见表1。 2.3 各组骨髓间充质干细胞培养上清液中微量丙二醛水平 应用氯化钴建立细胞缺氧模型,以芒果苷进行预保护,测定大鼠骨髓间充质干细胞缺氧损伤12 h及24 h的微量丙二醛水平,观察芒果苷对大鼠骨髓间充质干细胞缺氧损伤的保护作用。可见,芒果苷对大鼠骨髓间充质干细胞缺氧损伤的微量丙二醛作用呈时间依赖性及浓度依赖性。用200 μmol/L氯化钴处理细胞12,24 h,大鼠骨髓间充质干细胞微量丙二醛水平上升。20 μmol/L芒果苷组丙二醛水平低于200 µmol/L氯化钴组(P < 0.05),随着芒果苷浓度的升高,芒果苷对大鼠骨髓间充质干细胞的保护作用逐渐增强。40,80,160 μmol/L芒果苷组丙二醛水平与 200 µmol/L氯化钴组比较,差异有非常显著性意义(P < 0.01),见表2。 2.4 各组骨髓间充质干细胞培养上清液中过氧化氢酶活性 应用氯化钴建立细胞缺氧模型,以芒果苷进行预保护,测定大鼠骨髓间充质干细胞缺氧损伤12 h及24 h的过氧化氢酶活性,观察芒果苷对大鼠骨髓间充质干细胞缺氧损伤的保护作用。可见,芒果苷对大鼠骨髓间充质干细胞缺氧损伤的过氧化氢酶作用呈时间依赖性及浓度依赖性。用200 μmol/L氯化钴处理细胞12 h及24 h,大鼠骨髓间充质干细胞过氧化氢酶活性下降。处理12 h时20 μmol/L芒果苷组过氧化氢酶活性与200 μmol/L CoCl2组比较未见明显变化(P > 0.05),随着芒果苷浓度的升高,芒果苷对大鼠骨髓间充质干细胞的保护作用逐渐增强,40 μmol/L芒果苷组过氧化氢酶活性高于200 µmol/L氯化钴组(P < 0.05),80,160 μmol/L芒果苷组过氧化氢酶活性明显高于 200 µmol/L氯化钴组(P < 0.01)。处理24 h时20 μmol/L芒果苷组过氧化氢酶活性高于200 μmol/L 氯化钴组(P < 0.05);随着芒果苷浓度的升高,芒果苷对大鼠骨髓间充质干细胞的保护作用逐渐增强,40,80,160 μmol/L芒果苷组丙二醛水平与200 µmol/L氯化钴组比较,差异有非常显著性意义(P < 0.01),见表3。 2.5 各组骨髓间充质干细胞内活性氧变化 应用氯化钴建立细胞缺氧模型,以芒果苷进行预保护,测定大鼠骨髓间充质干细胞缺氧损伤3,6,12,24 h的细胞内活性氧变化,观察芒果苷对大鼠骨髓间充质干细胞缺氧损伤的保护作用。可见,芒果苷对大鼠骨髓间充质干细胞缺氧损伤的活性氧作用呈时间依赖性及浓度依赖性。用 200 μmol/L氯化钴处理细胞3,6,12,24 h,大鼠骨髓间充质干细胞活性氧上升,第3小时为最高值,随着时间的延长,活性氧水平逐渐下降。20 μmol/L芒果苷组活性氧水平低于200 µmol/L氯化钴组(P < 0.05),随着芒果苷浓度的升高,芒果苷对大鼠骨髓间充质干细胞的保护作用逐渐增强。40,80,160 μmol/L芒果苷组活性氧水平与 200 µmol/L氯化钴组比较,差异有非常显著性意义(P < 0.01),见表4。"
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