Effects of Wallerian degeneration on biological characteristics and secretory function of Schwann cells in rats with sciatic nerve injury

doi:10.3969/j.issn.2095-4344.2014.33.006

Abstract

Abstract:

BACKGROUND: Studies have shown that Schwann cells form a Bunger band in the basement tube and guide the extension of regenerating axons after peripheral nerve injury, but the exact mechanism remains to be explored.
OBJECTIVE: To explore the effect of Wallerian degeneration on biological characteristics and secretory function of Schwann cells in rats with sciatic nerve injury.
METHODS: A rat model of sciatic nerve injury was established and divided into two groups: sciatic nerve transection group and surgical control group. Schwann cells were isolated and cultured from sciatic nerve segments by one enzyme digestion. The cell morphology was observed under light microscope and S-100 protein expression was determined by immunofluorescence staining. After subculture, the first generation of Schwann cells were chosen to draw the growth curve by the counting method within 14 days. The cell activity was detected by MTT assay. The adhesion of Schwann cells was examined by acid phosphatase analysis and the concentration of nerve growth factor was detected by ELISA method.
RESULTS AND CONCLUSION: At 14 days after primary culture, a great number of Schwann cells were observed near the edges of nerve segments in the sciatic nerve transection group, but only small number of Schwann cells scattered around nerve segments in the control group. Schwann cells in both groups showed S-100 positive expression. At 3 days after subculture, Schwann cells reached the logarithm proliferative phase, the cell number and proliferation absorbance values in both groups were increased along with time extension. Furthermore, the number of Schwann cells and absorbance value in the sciatic nerve transection group were significantly higher than those of control group (P < 0.05). The adhesion ability in the sciatic nerve transection group was also significantly higher than those in the control group (P < 0.05). ELISA results showed that, the concentrations of nerve growth factor in the sciatic nerve transection group were significantly higher than those in the control group at 4, 6, 8, 10, 12 and 14 days (P < 0.05). After sciatic nerve injury, Wallerian degeneration can induce Schwann cells dedifferentiate into the precursors, significantly influence the biological function of Schwann cells, promote the proliferation of Schwann cells within the short term, secrete large amounts of neurotrophic factors, enhance cell adhesion, and provide a suitable microenvironment for regenerated axons. In addition, it creates the necessary microenvironment for peripheral nerve regeneration.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

全文链接：

Key words: sciatic nerve, nerve growth factor, Schwann cells

CLC Number:

R318

Li Yue-zhen, Wu Geng, Wu Yang, Jin Xiu-dong, Zhang Ji-fei, Zhao Fu-sheng. Effects of Wallerian degeneration on biological characteristics and secretory function of Schwann cells in rats with sciatic nerve injury[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(33): 5282-5287.

Figures/Tables 4

References

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