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    19 February 2014, Volume 18 Issue 8 Previous Issue    Next Issue
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    Nicotine effects on the osseointegration of implants with different treatments
    Sun Xin, Hou Yu-dong, Teng Teng, Xue Peng-fei, Liu Shun-zhen
    2014, 18 (8):  1149-1154.  doi: 10.3969/j.issn.2095-4344.2014.08.001
    Abstract ( 488 )   PDF (2121KB) ( 484 )   Save

    BACKGROUND: Studies have confirmed that nicotine affects the activity of osteoblasts, osteoclasts, fibroblasts and erythrocytes.
    OBJECTIVE: To study the nicotine effects on osseointegration and the expression of osteoprotegerin and bone morphogenetic protein 2 after implantation of dental implants with surface treatment by sandblasting or acid etching.
    METHODS: Twenty-four Sprague-Dawley rats were randomly assigned to two groups and received daily injections for 2 weeks as follows: Nicotine 2 mg/kg twice for experimental group, saline solution for control group. Then the titanium implants with surface sandblasting or acid etching were implanted into the tibiae followed by continuous nicotine or normal saline injection. At weeks 2 and 4 after implantation, the implants and surrounding bone tissue were prepared for CT, X-ray and hematoxylin-eosin staining examinations to evaluate bone healing and expression levels of bone-related genes were measured by quantitative RT-PCR.
    RESULTS AND CONCLUSION: Compared with the control groups, the degree of osseointegration and the expression of osteoprotegerin and bone morphogenetic protein 2 in the experimental groups were decreased significantly (P < 0.05), except that the expression of bone morphogenetic protein 2 in the experimental group with acid etching was not significantly reduced. In addition, the expression of bone morphogenetic protein 2 in the experimental group with acid etching was higher than that in the experimental group with sandblasting at 2 weeks after implantation (P < 0.05). The X-ray and CT show that the quantities of new generation bone and the degree of bone mineralization of the sandblasting group were significant lower than those of the acid etching group under the intervention of nicotine. Hematoxylin-eosin staining showed that the activity and quantity of osteoblasts around the implants down-regulated significantly, but acid etching-treated implants showed better outcomes than sandblasting-treated implants.


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    Effect of calcium phosphate cement/bone morphogenetic protein 6/vascular endothelial growth factor in bone defect repair
    Liao Hong-xing, Liu Zhan-liang, Zou Xue-nong
    2014, 18 (8):  1155-1160.  doi: 10.3969/j.issn.2095-4344.2014.08.002
    Abstract ( 282 )   PDF (2637KB) ( 674 )   Save

    BACKGROUND: Implantation of bone morphogenetic protein (BMP) or vascular endothelial growth factor (VEGF) alone, without support vectors, is easy to be flushed away by the blood flow, and thus limits the osteogenesis and angiogenesis.
    OBJECTIVE: To observe the effects of combination of calcium phosphate cement (CPC), BMP-6/VEGF in bone defect repair.
    METHODS: Defect models of the bilateral medial femoral condyle were prepared in New Zealand white rabbits. Then, the medial femoral condyle was filled with CPC/BMP-6/VEGF, CPC/BMP-6, and CPC, respectively, in the left side, but nothing in the right side as control. After 8 and 16 weeks of implantation, the hard tissue slices were prepared for histological observation and scanning electron microscope observation.
    RESULTS AND CONCLUSION: All three kinds of materials showed good biocompatibility, and no obvious inflammation was found. After 8 weeks of implantation, the junction of the CPC/BMP-6/VEGF and bone tissue was almost completely covered by newly formed trabecular bone. With the development of cement degradation, abundant osteoblasts could be found in the surface of newborn trabecular bone. After 16 weeks of implantation, an ongoing cement degradation and bone formation was seen. Moreover, newly formed bone tissue increased and became thicker. The cement in the interface was separated into small pieces and closely interconnected with the surrounding tissues, and newly formed bone showed a mesh-like ingrowth into the cement. This newly formed bone was mature and could not be distinguished from the original trabecular bone. Both the degradation and osteogenesis of CPC and CPC/BMP-6 were much slower than that of CPC/BMP-6/VEGF (P < 0.05). This study demonstrates angiogenesis and osteogenesis in vivo through the additive effects of VEGF and BMP-6. CPC/BMP-6/VEGF can be an ideal bone substitute in bone repair.


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    Biological properties of biphasic tricalcium phosphate bioceramics/calcium sulfate bone cement porous three-dimensional scaffolds  
    Tan Ying-yun, Bai Shi, Liao Yun-mao
    2014, 18 (8):  1161-1164.  doi: 10.3969/j.issn.2095-4344.2014.08.003
    Abstract ( 309 )   PDF (1618KB) ( 521 )   Save

    BACKGROUND: With the development of tissue engineering, porous bioceramics are more and more used to repair bone defects. Current research focuses on the biological synthesis of this bioceramics and its performance evaluation.
    OBJECTIVE: To discuss the preparation of a new kind of bone cement and to determine its physicochemical properties and biocompatibility with osteoblasts.
    METHODS: Biphasic tricalcium phosphate powders were prepared using co-precipitation method. The powder was turned into granular stuff by arabic gum. After sintering, porous hydroxyapatite/tricalcium phosphate bioceramics were harvested, and then mixed with alpha-hemihydrate to prepare the bone  
    cement.
    RESULTS AND CONCLUSION: X-ray diffraction confirmed that the synthetic substance was a kind of biphasic calcium phosphate ceramic having a porous structure. The bone cement could be in the plastic state within      3 minutes. The curing time was 15 minutes, and the curing temperature was 36.5 ℃. The maximum compression strength was 5.82 MPa, and the MTT toxicity was level 0. Osteoblasts could grew well on the material surface.


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    Chondrogenic co-culture of allogenic decalcified bone matrix and bone marrow mesenchymal stem cells in the joint cavity: comparison of cartilage traits in the same joint cavity
    Xu Bin, Zhou Liang, Wang Ying-ming, Qian San-xiang
    2014, 18 (8):  1165-1171.  doi: 10.3969/j.issn.2095-4344.2014.08.004
    Abstract ( 295 )   PDF (3181KB) ( 465 )   Save

    BACKGROUND: Loose bodies in the knee are found to survive for a long term and maintain certain histophysiological properties of cartilage tissue. Therefore, a bold hypothesis is proposed that the joint cavity may be a preferred environment for chondrocyte growth and development, supporting the concept of “intracavitary culture and intracavitary transplantation”.
    OBJECTIVE: To observe the trait difference of chondrogenic culture with allogenic decalcified bone matrix and bone marrow mesenchymal stem cells in the joint cavity or in vitro versus cartilage in the same cavity.
    METHODS: There were three groups in this experiment: in in vitro culture group, bone marrow mesenchymal stem cells from newborn rabbits undergoing chondrogenic culture were co-cultured with decalcified bone matrix from adult rabbits in vitro; in intracavitary culture group, bone marrow mesenchymal stem cells from newborn rabbits undergoing chondrogenic culture were co-cultured with decalcified bone matrix from adult rabbits in the joint cavity; normal cartilage in the same cavity served as control group.
    RESULTS AND CONCLUSION: (1) After 12 weeks of culture, in the in vitro culture group, hematoxylin-eosin staining showed a small amount of chondrocytes proliferated, with blue-stained nuclei; toluidine blue staining showed chondrocytes arranged disorderly, surrounded by a small amount of matrix; Masson staining showed a small positive area and irregular cell arrangement; type II collagen immunohistochemistry staining showed a few of yellow particles in the cytoplasm and extracellular matrix. (2) After 12 weeks of culture, in the intracavitary culture group, hematoxylin-eosin staining showed proliferation of chondrocytes with blue-stained nuclei; toluidine blue staining showed cluster-shaped arrangement of chondrocytes surrounded by the matrix with lacuna formation; Masson staining showed there were many positive cells with blue-stained matrix that arranged in a certain stress direction; immunohistochemical identification of type II collagen was positive, and brown-yellow stained particles could be discerned in the extracellular matrix. These findings indicate that tissue-engineered cartilage can be generated by co-culture of allogenic decalcified bone matrix and bone marrow mesenchymal stem cells in the joint cavity or in vitro, and the cartilage cultured in the joint cavity is more close to normal cartilage than that cultured in vitro.


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    Applicability and biodegradability in vivo upon polyglycolic acid nonwoven mesh and poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) porous sponge as scaffolds for tissue engineered cartilage
    Sun An-ke, Sun Jing, Sun Wei, Zhang He, Chen Wei, Hua Ze-quan
    2014, 18 (8):  1172-1178.  doi: 10.3969/j.issn.2095-4344.2014.08.005
    Abstract ( 318 )   PDF (2654KB) ( 637 )   Save

    BACKGROUND: Both polyglycolic acid (PGA) nonwoven mesh and poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHH) show good applicability, biodegradability and biocompatibility with porous sponges.
    OBJECTIVE: To explore the applicability and biodegradability in vivo of PGA nonwoven mesh and PHBHH porous sponge as scaffolds for tissue engineered cartilage.
    METHODS: Rabbit chondrocytes were seeded onto PGA nonwoven mesh and PHBHH porous sponge respectively as scaffolds to form chondrocyte-PGA or chondrocyte-PHBHH composites by tissue engineering technique. The composites were implanted subcutaneously into the dorsum of the adult New Zealand white rabbits as experimental group. In addition, PGA nonwoven mesh and PHBHH porous sponge respectively were implanted subcutaneously into the dorsum of another rabbits as control group.
    RESULTS AND CONCLUSION: Histological examination showed that for the tissue engineered cartilage using PGA nonwoven mesh as scaffolds a few smaller chondrocytes and some remainder of PGA fibers existed among the matrix of neocartilage after 4 weeks; near mature cartilage appeared without PGA fiber remainder after 8 weeks; then mature cartilage formed with a plenty of cartilage matrix and without PGA fiber remainder after 12 weeks. For the tissue engineered cartilage using PHBHH porous sponge as scaffolds, immature chondrocytes presented with more remainder of PHBHH similar to “foreign substance” in neocartilage after 4 weeks; and some remainder of PHBHH still remained after 8 weeks; up until 12 weeks, the remainder of PHBHH was invisible within the new cartilage. It was demonstrated to form rich cartilaginous matrix and type II collage through special staining and immunohistochemical test at 12 weeks after implantation. Both PGA nonwoven mesh and PHBHH porous sponge have good applicability and biodegradability as scaffolds for tissue engineered cartilage in vivo, and their biodegradability meets the requirement of constructing tissue engineered cartilage.


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    Cartilage construction in nude mice with microencapsulated stem cells derived from human umbilical cord Wharton’s jelly
    Yang Jian-hua, Liu Shu-yun, Zhao Peng, Lu Shi-bi, Zhang Li, Huang Jing-xiang, Zhao Bin,Xu Wen-jing, Guo Quan-yi
    2014, 18 (8):  1179-1184.  doi: 10.3969/j.issn.2095-4344.2014.08.006
    Abstract ( 338 )   PDF (2400KB) ( 510 )   Save

    BACKGROUND: Cartilage extracellular matrix with a large number of signaling molecule proteins and factors is likely to be an ideal material for tissue engineering cartilage.
    OBJECTIVE: To investigate the possibility of calcium alginate and cartilage extracellular matrix combined with microencapsulated stem cells derived from human umbilical cord Wharton’s jelly to construct ectopic tissue-engineered cartilage in nude mice.
    METHODS: Microfilament suspension of the cartilage extracellular matrix was prepared. Human stem cells derived from Wharton’s jelly of the umbilical cord were inoculated in to calcium alginate and cartilage extracellular matrix gel microspheres as experimental group. Stem cells derived from human umbilical cord Wharton’s jelly were incubated in simple alginate gel microspheres as control group. After in vitro culture, the microspheres were implanted into  the dorsal subcutaneous tissue of nude mice. Samples were taken after 4 weeks, respectively, for gross and histological observation.
    RESULTS AND CONCLUSION: The stem cells exhibited parallel-chondrocyte morphology in microspheres, which grew and proliferated quite well during in vitro culture. A new parallel-cartilaginous tissue was found in the subcutaneous tissue 4 weeks after surgery in the experimental group, and the tissue was positive for hematoxylin-eosin, safranine O, toluidine blue and collagen II. A large number of parallel-chondrocytes and cartilage lacuna-like structures were observed under a microscope with no obvious inflammatory reaction around the microspheres. The control group showed the partial degradation of microspheres, surrounded by only a small number of inflammatory cells and lymphocytes. Calcium alginate and cartilage extracellular matrix microspheres have a rather good histocompatibility which can be used to construct parallel-cartilaginous tissues by implanting stem cell-microspheric compound into the subcutaneous tissue of nude mice.


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    Vascularization of poly(hydroxybutyrate-co-hydroxyoctanoate) osteochondral scaffold in vitro
    Xiao Wei, Ren Wei, Zhang Yong-hong, Zhao Liang-qi
    2014, 18 (8):  1185-1190.  doi: 10.3969/j.issn.2095-4344.2014.08.007
    Abstract ( 309 )   PDF (2214KB) ( 453 )   Save

    BACKGROUND: The poly(hydroxybutyrate-co-hydroxyoctanoate) osteochondral scaffold which has been constructed in previous experiments has good biocompatibility and biodegradability and generates non-toxic degradation products.
    OBJECTIVE: To observe the vascularization of rabbit renal microvascular endothelial cells co-cultured with poly(hydroxybutyrate-co-hydroxyoctanoate) osteochondral scaffold.
    METHODS: The poly(hydroxybutyrate-co-hydroxyoctanoate) osteochondral scaffold having a three-layer structure (layer of bone/bone and cartilage interface layer/layer of cartilage) was prepared by solvent casting/particle leaching method. The renal microvascular endothelial cells at passage 3 were seeded onto the scaffold of bone layer. The proliferation of the renal microvascular endothelial cells growing on the scaffolds was examined using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide method, the growth of cells in the scaffold was observed by hematoxylin-eosin staining under electron microscope after 10 days.
    RESULTS AND CONCLUSION: The integrated osteochondral scaffold had a clear appearance of three-layer structure, which had closed connections between the three layers. Porous bone layer was visible as well as uniform and interlinked pores, and the porosity was 78%. The renal microvascular endothelial cells seeded onto the scaffold proliferated well and presented a three-dimensional growth after 10 days of co-culture, but there were no cells on the interface layer. Cells which adhered and grew between the pores of the bone layer were observed through hematoxylin-eosin staining. Cells showed a luminal-like structure growing on the scaffold with the porous structure, but they did not grow into the interface layer of bone and cartilage.


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    Construction of tissue-engineered cartilage with cross-linked sodium hyaluronate as scaffold materials in vitro
    Liu Shao-ying, Chen Jian-ying, Chen Qian-qian, Liu Fei, Liu Xia, Zhu Xi-qiang, Ling Pei-xue
    2014, 18 (8):  1191-1197.  doi: 10.3969/j.issn.2095-4344.2014.08.008
    Abstract ( 302 )   PDF (1143KB) ( 749 )   Save

    BACKGROUND: Tissue engineering provides new ideas and approaches for repair of cartilage defects.
    OBJECTIVE: To develop a complete set of solutions for construction of tissue engineered cartilage in vitro, with chondrocytes as seed cells and cross-linked sodium hyaluronate as scaffold materials.
    METHODS: New Zealand rabbit articular chondrocytes were isolated, counted, and then cultured and passaged to prepare cell suspension. Toluidine blue staining, RT-PCR and immunocytochemistry were exerted to evaluate the cultured cells. Chondrocytes were seeded and co-cultured with cross-linked sodium hyaluronate scaffold for 21 days. Then, RNA was isolated for RT-PCR, and frozen sections were prepared for morphological observation and immunohistochemistry study.
    RESULTS AND CONCLUSION: The chondrocytes could adhere to the cross-linked sodium hyaluronate scaffold and aggregate, growing between fibers or adhering to the scaffold in a monolayer manner. The transcripts of cartilage specific aggrecan gene and collagen type II alpha 1 gene and cartilage specific protein collagen type II were expressed in cell-scaffold complexes to maintain the phenotype of chondrocytes. Cell-scaffold complexes co-cultured in vitro can form cartilage extracellular matrix, by which tissue engineered cartilage is expected to be obtained.


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    Biocompatibility and osteoinductive activity of nano-hydroxyapatite/chitosan/ poly(lactide-co-glycolide) scaffolds in vitro
    Wang Fei, Zhou Hong, Guo Yu-cheng, Su Xiao-xia, Rao Guo-zhou, Zhao Xiao-peng
    2014, 18 (8):  1198-1204.  doi: 10.3969/j.issn.2095-4344.2014.08.009
    Abstract ( 370 )   PDF (1016KB) ( 750 )   Save

    BACKGROUND: Studies have found that combination of two of chitosan (CS), nano-hydroxyapatite (nHA) and poly(lactide-co-glycolide) (PLGA) can improve the mechanical properties and biocompatibility of the composite stent in certain extent as well as improve osteogenic differentiation of the cells, but there is a certain distance from the ideal bone tissue engineering scaffolds.
    OBJECTIVE: To study biocompatibility and osteoinductive activity of nHA/CS/PLGA scaffolds with different proportions in vitro.
    METHODS: nHA/CS/PLGA scaffolds were prepared at mass ratio of 10:10:80, 10:20:70, 20:10:70 respectively by particle leaching method. And human bone marrow stem cells (hBMSCs) were co-cultured with these scaffolds in vitro. Adhesion, proliferation, and osteoinductive activity of these scaffolds were examined qualitatively and quantitatively by growth curve of hBMSCs on scaffolds. Gene expression of alkaline phosphatase activity and osteocalcin was detected by RT-PCR.
    RESULTS AND CONCLUSION: hBMSCs could be attached, proliferated, and osteoinduced better on the nHA/CS/PLGA scaffold with the mass ratio of 20:10:70, compared to the other two groups of scaffolds. The differences were significant statistically (P < 0.05). Alkaline phosphatase and osteocalcin expressions were respectively higher in the scaffold with the mass ratio of 20:10:70 after 9-27 days of co-culture and 15-27 days of co-culture, in comparison with the other two groups of scaffolds. These findings indicate that the nHA/CS/PLGA scaffolds with the mass ratio of 20:10:70 demonstrated preferable biocompatibility and osteogenic inductivity, which is expected to be a promising scaffold material for bone tissue engineering.


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    Determination of ethyl pyruvate in chitosan nanoparticles by reversed-phase high-performance liquid chromatography
    Xu Feng-e, Zhao Bing-xin, Sun Shu-mao, Meng Yong-chun, Nan Kai-hui, Yu A-yong, Qu Jia
    2014, 18 (8):  1205-1210.  doi: 10.3969/j.issn.2095-4344.2014.08.010
    Abstract ( 725 )   PDF (763KB) ( 868 )   Save

    BACKGROUND: Research on ethyl pyruvate detection methods is reported rarely, and moreover, literature about reversed-phase high-performance liquid chromatography (RP-HPLC) for detection of ethyl pyruvate is less.
    OBJECTIVE: To establish an RP-HPLC method for determination of ethyl pyruvate in ethyl pyruvate-chitosan nanoparticles.
    METHODS: The chromatographic analysis was performed on a ZORBAX Eclipse XDB-C18 column (4.6 mm×
    150 mm, 5 μm) at 25 ℃, with the mixture of acetonitrile and water (40:60, V/V) as the mobile phase at the flow rate of 1 mL/min. The determination wavelength was set at 210 nm and the injection volume was 20 μL.
    RESULTS AND CONCLUSION: The peak of ethyl pyruvate and the peaks of auxiliary materials and solvent were separated well. The linear rang of ethyl pyruvate was 1-100 mg/L (r=0.999 6). The relative standard deviation of both the intra-and inter-day precision was less than 3% for low-, moderate-, and high-concentration ethyl pyruvate. The relative standard deviation of reproducibility test and stability test was 1.25% and 1.3%, respectively. Sample average recovery rates were (91.5±1.0)%, (3.5±0.2)%, (94.4±0.4)%, respectively. Encapsulation efficiency of samples were (87.2±0.22)%, (90.5±0.15)%, (91.1±0.17)%, respectively. The relative standard deviation of different sample content were 0.9%, 0.5%, 0.3%, respectively. The RP-HPLC method for determination of ethyl pyruvate is sensitive, accurate and highly specific with wide linear range and high sample average recovery.


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    Effect of water storage on the flexural properties of three dual-cure core buildup composite resins
    Li Ya-nan, Cai Qing, Jia Xiao-long, Deng Xu-liang, Yang Xiao-ping
    2014, 18 (8):  1211-1217.  doi: 10.3969/j.issn.2095-4344.2014.08.011
    Abstract ( 362 )   PDF (1573KB) ( 740 )   Save

    BACKGROUND: Hydrothermal condition of oral environment has an effect on the mechanical properties of dental core materials.
    OBJECTIVE: To evaluate effect of water storage on the weight change rates and flexural properties of three dual-cure core buildup composite resins.
    METHODS: Rectangular specimens (25 mm×2 mm×2 mm) were prepared from three dual-cure core buildup composite resins (LuxaCore Smartmix Dual, Para core and Clearfil DC Core). Specimens were stored in distilled water at 37 ℃ for 0, 1 day, 1, 2, 3 weeks and 1 month, respectively, weighted and then subjected to three-point bending test in a universal testing machine.
    RESULTS AND CONCLUSION: Weight change rates of the three composite resins increased with immersion time; at the same immersion time, weight change rates of three composite resins ranked as the following order: LuxaCore Smartmix Dual > ParaCore > Clearfil DC Core. The flexural strength of LuxaCore Smartmix Dual decreased with immersion time. The flexural strength of ParaCore showed no significant change during 3 weeks immersion (P > 0.05), while it showed a significant decreasing after 1 month immersion (P < 0.05). There was no significant change on the flexural strength of Clearfil DC Core in the whole immersion process(P > 0.05). The flexural modulus of LuxaCore Smartmix Dual showed a decreased trend in the whole immersion process, but it showed that there was no significant change during the first 2 weeks(P > 0.05), while a significant decrease occurred after 3 weeks and 1 month (P < 0.05). Compared with original specimens, the flexural modulus of ParaCore and Clearfil DC Core showed a decreased trend, but this change was not significant (P > 0.05); the flexural properties of three composite resins showed a fluctuant decreased trend along with weight changes increased. To sum up, Clearfil DC Core showed the best water resistance among three composite resins.


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    Feasibility of repairing collapsed crown and fixed bridge by porcelain jacket crown
    Gu Zhen-yu, He Hui-yu, Cai Tian-heng, Tian Yuan
    2014, 18 (8):  1218-1223.  doi: 10.3969/j.issn.2095-4344.2014.08.012
    Abstract ( 329 )   PDF (2133KB) ( 469 )   Save

    BACKGROUND: Porcelain jacket crowns have achieved good results in the repair of porcelain ceramic crown and bridge collapse.
    OBJECTIVE: To test the bond strength of single porcelain jacket crown, thus determining the feasibility of clinical application of repairing collapsed crown and fixed bridge by porcelain jacket crown, and meanwhile to discuss the influence between blastsanding and silane coupling agent treatment on the metal bonding strength in the repair of porcelain fracture.
    METHODS: The porcelain jacket crown was cast in the same shape from disk-shaped waxpatterns. Twenty specimens were chosen without blowholes and casting defects and they were all routinely burnished and cleaned in an ultrasonic bath and dried using an oil-free air stream stand-by. Then we randomly divided the specimens into four groups: specimens were treated with nothing (control), sandblast, silane coupling agent and sandblast+silane coupling agent, respectively. Each of the specimen was bonded with Poly-F Plus. AG-10TA universal testing machine was used to test the tensile strength.
    RESULTS AND CONCLUSION: The tensile bond strength of control, sandblast, silane coupling agent and sandblast+silane coupling agent groups respectively were (0.68 0.04), (1.00 0.02), (1.31 0.08), and (1.09 0.04) kN. There were significant differences between groups (P < 0.01). In the control group, the metal surface was smooth, showing some irregular small superficial veins. In the sandblast group, the metal surface showed irregular scaly protrusions conducive penetrate adhesives. In the silane coupling group, the metal surface manifested a honeycomb structure in favor of chimeric adhesives. The sandblast+silane coupling agent group showed some metal 
    projections ranging in size, on the rough metal surface, which was conducive to bond. Porcelain jacket crown applied to repair collapsed crown and fixed bridge is feasible. Sandblasting and silane coupling agent can improve bond strength of porcelain jacket crown. The bond strength of metal specimens treated with silane coupling agent is the best followed by the sandblasting+silane coupling agent.


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    Synthesis and characterization of lanthanum-incorporated hydroxyapatite coatings
    Lou Wei-wei, Dong Yi-wen, Jin Yi-fan, Liu Jin-song
    2014, 18 (8):  1224-1230.  doi: 10.3969/j.issn.2095-4344.2014.08.013
    Abstract ( 430 )   PDF (972KB) ( 584 )   Save

    BACKGROUND: Hydroxyapatite-coated titanium substrates improve the biocompatibility and have the property of intimating ossteointegration with host bonebed. However, hydroxyapatite lacks the necessary mechanical strength and degrades easily in the extracellular fluids, which may affect the stability of the titanium implant.
    OBJECTIVE: To study the synthesis and characterization of lanthanum-incorporated hydroxyapatite coatings.
    METHODS: Lanthanum-incorporated hydroxyapatite coatings were prepared by hydroxyapatite and 10%, 20% and 30% lanthanum, respectively, by means of sol-gel, which were then deposited on titanium substrates with dip-withdrawal technique. Surface morphology and crystalline microstructure of the coatings were observed by scanning electron microscope. The presence of functional groups for the obtained samples was performed by Fourier transform infrared absorption spectroscopy and X-ray diffraction. The Ca2+ concentration released from the coatings was measured by atomic absorption spectrometry for analysis of degradation property.
    RESULTS AND CONCLUSION: With the increase of lanthanum content, the diffraction peak and crystallinity of lanthanum-incorporated hydroxyapatite coatings were increased, but the whole structure of lanthanum-incorporated hydroxyapatites had little changes. The crystal structure maintained stable with charge balance. The lanthanum-incorporated hydroxyapatite coatings showed uniform and high-dense structure and were free of cracks, indicating the coatings had good bonding strength. Under the simulated biological environment, based on the determination of Ca2+ release from the coatings, we can conclude the lanthanum-incorporated hydroxyapatite coatings have a stronger acidoresistance.


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    Insulin-like growth factor-1/chitosan/collagen composite scaffold and the proliferation of human periodontal ligament cells
    Zhao Bo, Wang Yong-lan
    2014, 18 (8):  1231-1236.  doi: 10.3969/j.issn.2095-4344.2014.08.014
    Abstract ( 233 )   PDF (799KB) ( 604 )   Save

    BACKGROUND: Insulin-like growth factor-1 can promote mitosis of fibroblasts as well as periodontal cell growth, differentiation and synthesis of extracellular matrix.
    OBJECTIVE: To observe the effects of chitosan/collagen composite scaffold carrying insulin-like growth factor-1 on proliferation of human periodontal ligament cells.
    METHODS: The human periodontal ligament cells were seeded on chitosan/collagen composite scaffold carrying insulin-like growth factor-1 and ordinary collagen scaffold. The release of recombinant human transforming growth factor-β1 was detected at 1, 24 hours and 1 week after culture; cell adhesion and proliferation were detected at days 1, 7 and 28.
    RESULTS AND CONCLUSION: The release rate of recombinant human transforming growth factor-β1 in the composite scaffold was significantly lower than that in the ordinary collagen scaffold at 1, 24 hours and 1 week after cell seeding (P < 0.01). The cell adhesion and proliferation showed no difference between two groups at day 1 after cell seeding, but became significantly higher in the composite scaffold than that in the ordinary collagen scaffold at days 7 and 28 (P < 0.01). These findings indicate that chitosan/collagen composite scaffold carrying insulin-like growth factor-1 can significantly promote the proliferation of the human periodontal ligament cells.


    中国组织工程研究杂志出版内容重点:生物材料;骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性;组织工程


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    Stability-related factors and individualized weight-bearing scheme for Bego implants
    Chen Hui, Zhou Feng-mei, Gu Xiao-xia, Wu Ping-qun
    2014, 18 (8):  1237-1243.  doi: 10.3969/j.issn.2095-4344.2014.08.015
    Abstract ( 562 )   PDF (659KB) ( 458 )   Save

    BACKGROUND: There are some controversies on the choice of immediate or delayed weight-bearing schemes of implants.
    OBJECTIVE: To explore the weight bearing scheme for Bego implants based on implant stability quotient (ISQ) measured by Osstell, and to analyze the factors which influence implants’ stability.
    METHODS: Seventy-four single Bego implants with ISQ ≥ 50 in 62 patients were selected and randomly divided into test group (36 single Bego implants in 31 patients) and control group (38 single Bego implants in 31 patients). Patients in the test group were given immediate loading, and patients in control group were given delayed loading. Two groups’ ISQ of Bego implants after loading (1, 2, 3, 4, 6, 8 and 12 weeks) were compared, and factors which influence implants’ stability were analyzed.
    RESULTS AND CONCLUSION: The lowest ISQ for immediate loading of the test group appeared in 2-3 weeks after loading, and that of the control group appeared in 3-4 weeks. There was no statistical difference between two groups on ISQ of Bego implants after bearing (1, 2, 3, 4, 6, 8 and 12 weeks) (P > 0.05). Multiple regression analysis showed that the factors including age, types of osseous substance and implant length were positively related with stability of Bego implants, yet oral hygiene was negatively related with stability of Bego implants. Immediate loading and delayed loading have similar stability to single Bego implants with ISQ ≥ 50 measured by Osstell, so individualized weight bearing scheme may be selected according to patient’s own condition under the guidance of Osstell, further to improve implants’ success rate after loading.


    中国组织工程研究杂志出版内容重点:生物材料;骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性;组织工程


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    Preparation and biocompatibility of gamma-aminopropyl triethoxysilane/polypyrrole/ polyester monofilament
    Wang Ying, Chen Ying, Yue Bing-fei, Tang Jin-tian, Li Ying, Zhang Xiao-na
    2014, 18 (8):  1244-1249.  doi: 10.3969/j.issn.2095-4344.2014.08.016
    Abstract ( 480 )   PDF (2525KB) ( 497 )   Save

    BACKGROUND: Now most of the researches on transplant hair are based on the patients with enough hair, but there is no apparent corrective effect for large area of alopecia. Implanted artificial hairs can solve this problem.
    OBJECTIVE: To make and evaluate the γ-aminopropyl triethoxysilane/polypyrrole/polyester (KH-550/PPy/PET) composite monofilament as implanted artificial hairs, and to carry out cytotoxicity tests using NCTC clone 929 cells with composite monofilament.
    METHODS: KH-550/PPy/PET composite monofilament was prepared in a series of steps, including pre-spotting, alkali treatment, silane coupling agent treatment and polypyrrole coating. The viability of NCTC clone 929 cells were detected after 1, 2, 3, 5, 7 days of co-culture with composite monofilament by using cell counting kit-8.
    RESULTS AND CONCLUSION: KH-550/PPy/PET composite monofilament had a smooth surface without crack. The PPy films did not come off accidentally and had good wearability. After alkali treatment, PPy quality on the surface of monofilament was significantly heavier than before. Using silane coupling agent (KH-550) could effectively enhance biocompatibility and binding force of polyester monofilaments. After co-cultured with composite monofilaments, the viability of NCTC clone 929 cells was 100%, 80.37%, 73.26%, 81.96%, 77.50% at days 1, 2, 3, 5, 7 respectively. The level of cytotoxicity was grade 1. The results show that KH-550 can effectively enhance the binding force between PPy and PET monofilament, and the prepared KH-550/PPy/PET composite monofilament has good biocompatibility and no acute toxicity.


    中国组织工程研究杂志出版内容重点:生物材料;骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性;组织工程


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    Preventive effect of carboxymethyl chitosan solution on postoperative peritoneal adhesions in a rat model
    Cui Juan-juan, Feng Zhan-qin, Zhang Shou-qiang, Zheng Zeng-juan, Zhang Wei-fen
    2014, 18 (8):  1250-1256.  doi: 10.3969/j.issn.2095-4344.2014.08.017
    Abstract ( 635 )   PDF (3267KB) ( 594 )   Save

    BACKGROUND: The patients are suffering from peritoneal adhesions that are caused after abdominal operation. As so far, there is still no effective drug or method that can completely prevent peritoneal adhesions. Carboxymethyl chitosan is a biocompatible and biodegradable biomedical material with anti-adhesion effects, which is an ideal biomaterial for prevention of peritoneal adhesion theoretically.
    OBJECTIVE: To investigate the novel anti-adhesion properties of carboxymethyl chitosan anti-adhesion solution on the prevention of postsurgical adhesion in vivo in a rat model.
    METHODS: Fifty-six adult male Wistar rats were randomly divided into four groups: 0.9% normal saline solution (group A), hyaluronic acid gels (group B), medical chitosan gels (group C) and carboxymethyl chitosan anti-adhesion solution (group D). The model of postoperative intestinal adhesion was established by making cecal scratches/abdominal wall defects. All the rats were scarified after 2 or 3 weeks. Whole blood was collected by cardio-puncture, lung tissue and tissue adhesion were stripped. The incidence and degree of adhesions, histological effects, expression of transforming growth factor-β1 (TGF-β1), the amounts of hydroxyproline and white blood cells were observed.
    RESULTS AND CONCLUSION: The formation of postsurgical adhesions in groups B, C and D was significantly decreased, which was lighter than that of group A (P < 0.05). Furthermore, the adhesion formation in group D was significantly decreased in comparison with group A (P < 0.01). At the same time, the levels of transforming growth factor-β1, hydroxyproline and white blood cells in group D were lighter than those of group A (P < 0.05), and the histopathological results indicated that a marked reduction in inflammatory cells and fibroblasts. Carboxymethyl 
    chitosan anti-adhesion solution can effectively reduce the degree and incidence of postoperative adhesion, and it is becoming a promising drug delivery system in the context of postsurgical anti-adhesion.


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    Surface roughness and hardness of macromolecule ocular prosthesis materials
    Yang Xin, Song Yan, Wang Fan-tao, Wang Peng, Wang Chang-lei
    2014, 18 (8):  1257-1262.  doi: 10.3969/j.issn.2095-4344.2014.08.018
    Abstract ( 738 )   PDF (786KB) ( 661 )   Save

    BACKGROUND: The physical properties of macromolecule ocular prosthesis materials, such as density, hardness, mechanical strength, can affect the working accuracy of ocular prosthesis and can also affect the surface roughness of ocular prosthesis, thus affecting the comfort when the patients wear.
    OBJECTIVE: To study the differences of different macromolecule ocular prosthesis materials in bubble generation rate, hardness and surface roughness.
    METHODS: Thirty-seven test pieces, 30 mm×30 mm×2 mm, were made and then divided to seven groups according to their brands, called groups A, B, C, D, E, F, G, each group of five pieces. One surface of each test piece was polished in accordance with the principle of coarse to fine, the surface roughness of the two surfaces and hardness on the polished surface were measured, and then the bubble formation rate of the material was calculated.
    RESULTS AND CONCLUSION: The surface roughness values of groups A-G were (0.078±0.016), (0.074±0.019), (0.075±0.022), (0.066±0.020), (0.075±0.017), (0.068±0.015), and (0.067±0.017) μm, respectively. There was no significant difference in the surface roughness between the groups (P > 0.05). The hardness values of groups A-G were 766.92±3.71, 771.84±14.51, 791.20±9.64, 804.50±4.49, 779.00±17.92, 772.20±19.18, 704.00±7.23, respectively. There was a significant difference in the hardness between groups D, G and the other groups (P < 0.05) as well as between group D and group G (P < 0.05), but there was no significant difference 
    between the other groups (P > 0.05). The bubble generation rates of groups A-G were (8.87±0.29)%, (8.29±1.02)%, (6.94±0.43)%, (4.83±0.20)%, (7.59±0.19)%, (8.61±0.25)%, (4.89±0.17)%, respectively. There was a significant difference in the bubble generation rate between groups D, G and the other groups (P < 0.05), but there was no significant difference between group D and group G (P > 0.05), as well as between the other groups (P > 0.05). To a certain extent, the smaller the bubble formation rate is, the greater the hardness and the smaller the surface roughness are.


    中国组织工程研究杂志出版内容重点:生物材料;骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性;组织工程


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    Tissue-engineered soft tissue oriented manufacturing technologies and additive manufacturing
    Lian Qin, Li Di-chen, Chen Cheng, Zhang Wei-jie, Liu Ya-xiong, He Jian-kang, Jin Zhong-min
    2014, 18 (8):  1263-1269.  doi: 10.3969/j.issn.2095-4344.2014.08.019
    Abstract ( 515 )   PDF (696KB) ( 1371 )   Save

    BACKGROUND: In recent years, many manufacturing techniques have been recently developed for soft tissue engineering scaffolds. Especially additive manufacturing with a unique material accumulated forming principle can be feasible and reliable to manufacture the highly precise scaffolds with gradient structures and multi-materials for large soft tissue defect repairing.  
    OBJECTIVE: To summarize scaffolds manufacturing technologies in the soft tissue engineering applications developed in recent years and to predict the direction of development.
    METHODS: A retrieval was performed for the literature about the manufacturing methods of soft tissue scaffolds using key words of “additive manufacturing, microfabrication, vascular tissue engineering, muscle tissue engineering, cartilage tissue engineering, stereolithography, 3D printing, biodegradable hydrogel” in English and Chinese, which were published between January 2010 and September 2013 in PubMed Database and China National Knowledge Infrastructure (CNKI) Database.
    RESULTS AND CONCLUSION: For large soft tissue defects repairing, structure design of the scaffolds has been shifted from a simple planar structure to a more complex three-dimensional structure, and integration of scaffold structure, materials and cells, and growth factors during the manufacturing procedure can be used to obtain the resolution of vascularization. Additive manufacturings become one of the most promising approaches for the ideal soft tissue scaffolds with gradient and complex structure and multi-materials. In particular, the hydrogel/cell composite scaffolds fabrication, a hot but promising approach to develop the soft tissue engineering will be made progress by the accurate principles and processes of the hydrogel additive manufacturing combined with the introduction of living cells and growth factors.


    中国组织工程研究杂志出版内容重点:生物材料;骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性;组织工程


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    Application situation of degradable coronary stents
    Li Lu-feng, Liu Huan-yun, Zhao Xiao-hui
    2014, 18 (8):  1270-1276.  doi: 10.3969/j.issn.2095-4344.2014.08.020
    Abstract ( 790 )   PDF (805KB) ( 2112 )   Save

    BACKGROUND: The appearance of biodigradable stents brings a new dawn for the fourth coronary intervention revolution. They not only can solve the problem of postoperative acute occlusion of blood vessels, but also can be completely absorbed after a certain time.
    OBJECTIVE: To summarize the application status of biodegradable coronary stents.
    METHODS: PubMed, CBM and embase were searched for articles related to biodegradable intravascular stents.
    RESULTS AND CONCLUSION: Biodegradable polymer stents, biodegradable magnesium stents and biodegradable iron stents are currently the three major research biodegradable stent systems. Numerous clinical trials have demonstrated the long-term safety and reliability of the biological degradation stents, and in the near future they will replace the existing drug-eluting stents as the primary means of percutaneous coronary intervention. Biodegradable stents currently still have their limitations, which are reflected in the relationship between mechanical properties and degradation rate and cannot be applied to complicated coronary patients temporarily. It takes 6-12 months to restore normal physiological function of blood vessels, and it can be considered reasonable that degradation of stents is completed in 12-24 months. Poly(lactic-co-glycolic) produced by polylactic acid and polyglycolic acid is currently widely recognized as the stent’s framework. We can get a more appropriate balance between the mechanical properties of the stent and the degradation rate by adjusting the ratio of polylactic acid and polyglycolic acid. This stent not only has good mechanical properties, but also can be completely biodegradable after the restoration of normal function of blood vessels, which has a broad research space.


    中国组织工程研究杂志出版内容重点:生物材料;骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性;组织工程


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    Clinical bioeffects of buccal multiloop appliance
    Zhang Wan-nan, Hou Lu, Xu Shi-qian
    2014, 18 (8):  1277-1282.  doi: 10.3969/j.issn.2095-4344.2014.08.021
    Abstract ( 522 )   PDF (677KB) ( 434 )   Save

    BACKGROUND: Buccal multiloop appliance in the clinical treatment of Class III malocclusion has good results, but also exhibits some problems and deficiencies in the application process.
    OBJECTIVE: To summarize the clinical problems and corresponding solutions of buccal multiloop appliance.
    METHODS: A computer-based search of Wanfang, CNKI and PubMed databases were performed for articles related to buccal multiloop appliance published from 2000 to 2013. The key words were "buccal multiloop, Class III malocclusion, clinical application" in Chinese and English.
    RESULTS AND CONCLUSION: Compared with other appliances, the buccal multiloop appliance appears to have some unique advantages. The buccal multiloop appliance with the soft elastic role cannot only promote growth and development of the maxillary, but also inhibit the excessive growth of the maxillary and trigger mandibular functional shift, thereby achieving crossbite correction. Therefore, the buccal multiloop appliance can have good achievements in the clinical crossbite correction of the deciduous and permanent teeth. For patients with Class III malocclusion, the buccal multiloop appliance also shows good results, and the corresponding clinical problems and deficiencies appear to be resolved one by one accordingly. So the buccal multiloop appliance is highly efficient for crossbite correction.


    中国组织工程研究杂志出版内容重点:生物材料;骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性;组织工程


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    Meta-analysis of fibrin glue used in thyroid surgery
    Hua Dan, Guo Zi-jun
    2014, 18 (8):  1283-1288.  doi: 10.3969/j.issn.2095-4344.2014.08.022
    Abstract ( 372 )   PDF (711KB) ( 490 )   Save

    BACKGROUND: Fibrin glue can function to close the wound tissue, reduce leakage, stop bleeding, promote wound healing and prevent adhesion.
    OBJECTIVE: To systematically review the application of fibrin glue in thyroid surgery.
    METHODS: We searched China Journal Net database, Wanfang database, VIP database, Chinese Biomedical Database Online to retrieve clinical randomized controlled trials related to fibrin glue applied in thyroid surgery from January 2000 to April 2013. Included studies were analyzed using Rev Man 5.2 statistical software for Meta-analysis.
    RESULTS AND CONCLUSION: A total of six studies including 797 cases were enrolled in result analysis. Meta-analysis showed that the drainage volume at postoperative day 1 and total postoperative drainage volume were higher in the fibrin glue group than the control group (without fibrin glue) [odds ratio=-27.36, 95% confidence interval (-33.86, -20.87), P < 0.00001; weighted mean difference=-38.73, 95% confidence interval (-44.78, -32.67), P < 0.000 01]. The suture removal time was shorter in the fibrin glue group than the control group [odds ratio=-2.00, 95% confidence interval (-2.17, -1.83), P < 0.000 01]. No significant difference was found in the postoperative 3-day fever incidence [odds ratio=1.53, 95% confidence interval (0.59,3.96), P=0.38], wound infection [odds ratio=0.86, 95% confidence interval (0.12, 6.15), P=0.88], and postoperative hematoma [odds ratio=0.86, 95% confidence interval (0.21, 3.48), P=0.83] between the two groups. These findings indicate that fibrin glue used in thyroid surgery can significantly reduce drainage by non-increasing postoperative 
    complications.


    中国组织工程研究杂志出版内容重点:生物材料;骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性;组织工程


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    Replantation of inactivated tumor segment boiled in 70 ℃ distilled water for 15 minutes in the treatment of proximal fibular tumors
    Bao Yi-min, Zhang Guo-liang, Du Pei-jin, Wang Yue-wen
    2014, 18 (8):  1289-1294.  doi: 10.3969/j.issn.2095-4344.2014.08.023
    Abstract ( 395 )   PDF (704KB) ( 835 )   Save

    BACKGROUND: Apart from a complete resection of tumor tissues, the therapeutic goal for bone tumors is to maximize the repair and restoration of tissue defects and damaged functions. Autologous inactivated bone graft is of practical value for transplantation and reconstruction.
    OBJECTIVE: To investigate the effect of improved Malawer method combined with replantation of inactivated tumor segment in the treatment of proximal fibular tumors.
    METHODS: Since 2007, five patients with proximal fibular tumors (six sides) were admitted in the Affiliated Hospital of Inner Mongolia Medical University. These five patients were subjected to distal femoral amputation, intralesional curettage and bone grafting, upper fibular osteotomy + tumor segment heating (boiling in 70 ℃ distilled water for 15 minutes) + inactivated bone replantation. Therapeutic effects were followed.
    RESULTS AND CONCLUSION: One case of amputation had no recurrence and systemic metastasis during 4 years of follow-up; one case of intralesional curettage and bone grafting relapsed 1 year after operation; three cases of upper fibular osteotomy + tumor segment heating + inactivated bone replantation followed for 5 months recovered walking function and exhibited good healing of the outer structure of the knee, showing non-healing of inactivated tumor segment, non-remarkable callus formation, no local tumor recurrence, no loosening of internal fixation, and inactivated bone without fracture and absorption. These results confirmed that the tumor curettage and amputation both have obvious limitations; based on Malawer method, the replantation of inactive tumor segment can better ensure the structure integrity of the lower leg and in suit reconstruct the lateral biceps femoris tendon and the lateral collateral ligament, but genetically the revascularization and healing of inactivated bones will be difficult and slow with temperature increases, and therefore the period of postoperative brace protection should be relatively extended, allowing time delay of strenuous exercise and avoiding complications such as fractures.


    中国组织工程研究杂志出版内容重点:生物材料;骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性;组织工程


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    Vacuum-sealing drainages based on biological versus synthetic dressings in wound healing of skin grafting
    Liu Xiao-zhou, Xu Hai-dong, Zhao Jian-ning
    2014, 18 (8):  1295-1300.  doi: 10.3969/j.issn.2095-4344.2014.08.024
    Abstract ( 618 )   PDF (789KB) ( 586 )   Save

    BACKGROUND: Vacuum-sealing drainage in wound skin grafting can close wound, reduce the leakage, promote granulation hyperplasia and improve skin new speed.
    OBJECTIVE: To compare the clinical effects of two kinds of wound surface dressings used in wound skin grafting.
    METHODS: Eighty patients who needed skin grafting for trauma were retrospectively studied and chosen from September 2010 to March 2012. They were divided into two groups: biomaterial group (dressings mainly made of luffa fibers) and synthetics group (seaweed polyvinyl alcohol foam). The vacuum-sealing drainage device and wound dressing were removed after 1 week treatment. The skin grafting coverage and wound healing time were recorded.
    RESULTS AND CONCLUSION: All patients of two groups got effective skin grafting coverage rate, and no patient appeared with infection and nonunion. The skin grafting coverage and wound healing time of the biomaterial group significantly differed from the synthetics group (P < 0.05). These findings indicate that compared to the synthetic material group, the biomaterial group can be better for effective drainage, good circulation, bacteria inhibition and fast repair.


    中国组织工程研究杂志出版内容重点:生物材料;骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性;组织工程


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    Subcutaneous effusion after digital shaping titanium mesh cranioplasty
    Zhou Jun-ge, Qiu Yong, Cen Bo, Jiang Yong
    2014, 18 (8):  1301-1306.  doi: 10.3969/j.issn.2095-4344.2014.08.025
    Abstract ( 1460 )   PDF (791KB) ( 854 )   Save

    BACKGROUND: Subcutaneous effusion often occurs after digital shaping titanium mesh cranioplasty, and affects therapeutic effects. 
    OBJECTIVE: To explore the causes and corresponding prevention measures of subcutaneous effusion after digital shaping titanium mesh cranioplasty. 
    METHODS: We retrospectively analyzed the clinical data and treatment methods of 19 cases of subcutaneous effusion after digital shaping titanium mesh cranioplasty, summarized the postoperative complications and explored the effective methods for prevention and treatment of subcutaneous effusion.
    RESULTS AND CONCLUSION: After active treatment, five cases of dural breakage, four cases of foreign body stimulation, three cases of getting out of bed early, three cases of early extubation, three cases of long-time operation repair, and one case of excessive use of electric knife were all cured. Dural breakage and foreign body stimulation is considered as the main causes of postoperative effusion. Patients with subcutaneous effusion were given releasing elastic bandage, aspiration, and strict bed rest. After these active treatments, patients were all cured. Subcutaneous effusion may result from single or mixed factors. The above-mentioned causes are only a part. Non-central suspension, incomplete hemostasis, and preoperative excessive collapse of the bone window are all reported to be the reasons for the occurrence of subcutaneous effusion.


    中国组织工程研究杂志出版内容重点:生物材料;骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性;组织工程


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    Application of the collagen sponge artificial dura in severe traumatic brain injury
    Qin Guo-qiang, Wang Guan, Yan Cheng-fen, Peng Cai-zu, Shi Bo, Huang Wei-min
    2014, 18 (8):  1307-1312.  doi: 10.3969/j.issn.2095-4344.2014.08.026
    Abstract ( 520 )   PDF (657KB) ( 747 )   Save

    BACKGROUND: Craniotomy for severe traumatic brain injury is required to maintain the integrity of the dura mater. The artificial dura mater is now a common dural repair material, and looking for the ideal artificial dura mater is the exploring direction of neurosurgery.
    OBJECTIVE: To explore the application of collagen sponge artificial dura in severe traumatic brain injury
    METHODS: A retrospective analysis of 96 patients with severe head injury was performed, including 32 cases of the artificial dura with tightly suturing as the control group, and 64 cases of the artificial dura of collagen sponge without suturing as the experimental group. Operating time for hematoma clearance, blood loss, postoperative mechanical ventilation time, ICU monitoring time, the total number of hospitalized days as well as time interval from hematoma clearance to cranioplasty, operative time for cranioplasty, blood loss, and Glasgow Coma Scale scores after dural damage and 6 months postoperatively in the two groups were measured.
    RESULTS AND CONCLUSION: The same purpose was achieved in the two groups. The amount of bleeding during hematoma clearance, postoperative mechanical ventilation time, monitoring time in ICU, the total number of hospitalized days and Glasgow Coma Scale score of 6 months postoperatively showed no significant difference between the two groups (P > 0.05). But operative time for hematoma clearance and cranioplasty as well as blood loss in the second operation were statistically significant between two groups (P < 0.05). The collagen sponge artificial dura in severe traumatic brain injury can fully play a good role in reducing intracranial pressure, keeping brain functions, shortening operative time, and improving outcomes of patients, which has similar effects to tightly suturing the dura and creates favorable conditions for the following cranioplasty.


    中国组织工程研究杂志出版内容重点:生物材料;骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性;组织工程


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