Insulin-like growth factor-1/chitosan/collagen composite scaffold and the proliferation of human periodontal ligament cells

doi:10.3969/j.issn.2095-4344.2014.08.014

Abstract

Abstract:

BACKGROUND: Insulin-like growth factor-1 can promote mitosis of fibroblasts as well as periodontal cell growth, differentiation and synthesis of extracellular matrix.
OBJECTIVE: To observe the effects of chitosan/collagen composite scaffold carrying insulin-like growth factor-1 on proliferation of human periodontal ligament cells.
METHODS: The human periodontal ligament cells were seeded on chitosan/collagen composite scaffold carrying insulin-like growth factor-1 and ordinary collagen scaffold. The release of recombinant human transforming growth factor-β1 was detected at 1, 24 hours and 1 week after culture; cell adhesion and proliferation were detected at days 1, 7 and 28.
RESULTS AND CONCLUSION: The release rate of recombinant human transforming growth factor-β1 in the composite scaffold was significantly lower than that in the ordinary collagen scaffold at 1, 24 hours and 1 week after cell seeding (P < 0.01). The cell adhesion and proliferation showed no difference between two groups at day 1 after cell seeding, but became significantly higher in the composite scaffold than that in the ordinary collagen scaffold at days 7 and 28 (P < 0.01). These findings indicate that chitosan/collagen composite scaffold carrying insulin-like growth factor-1 can significantly promote the proliferation of the human periodontal ligament cells.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

全文链接：

Key words: biocompatible materials, insulin-like growth factor I, chitosan, collagen

CLC Number:

R318

Zhao Bo, Wang Yong-lan. Insulin-like growth factor-1/chitosan/collagen composite scaffold and the proliferation of human periodontal ligament cells[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(8): 1231-1236.

Figures/Tables 2

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