Effects of fish scale collagen membrane on adhesion, proliferation and osteogenic differentiation of rat bone marrow mesenchymal stem cells

doi:10.3969/j.issn.2095-4344.3094

Abstract

Abstract: BACKGROUND: In recent years, fish derived collagen has been regarded as a potential substitute for mammalian collagen, and its research in biomedical materials has gradually become a hot spot. Fish scale collagen materials with preserved fish scale shape have excellent mechanical properties, and there is no research to explore whether this material has the potential of bone tissue regeneration.
OBJECTIVE: To study the effect of fish scale collagen membrane on the adhesion, proliferation and osteogenic differentiation of rat bone marrow mesenchymal stem cells.
METHODS: The collagen membrane was prepared from Ctenopharyngodon idellus scales. The structure of collagen membrane was observed by naked eye and scanning electron microscope. The type of collagen was identified by Fourier transform infrared spectroscopy. The denaturation temperature of collagen membrane was determined by differential scanning calorimetry. Rat bone marrow mesenchymal stem cells at passage 3 were cultured in vitro on collagen membrane as experimental group; and those received conventional cell adherent culture were used as control group. The adhesion of cells on the collagen membrane of fish scale was observed by scanning electron microscope. The proliferation activity of cells in each group was detected by CCK-8, and the osteogenic differentiation ability of cells in each group was detected by alkaline phosphatase staining.
RESULTS AND CONCLUSION: (1) The fish scale collagen membrane was square and round, the light transmittance was high, and the “annual ring” pattern could be seen on the surface. Scanning electron microscope showed that the fish scale collagen membrane was multi-lamellar structure, which was composed of type I collagen and hydroxyapatite, and the denaturation temperature was 50.55 ℃. (2) The adhesion of bone marrow mesenchymal stem cells on the surface of fish scale collagen membrane was good, and the cell proliferation was better than that of the control group. The alkaline phosphatase activity of the surface cells of fish scale collagen membrane was higher than that of the control group, and the difference was statistically significant. (3) Results suggested that bone marrow mesenchymal stem cells on the surface of fish scale collagen membrane have good adhesion, which can promote cell proliferation and osteoblast differentiation, and has good biocompatibility and the potential of bone tissue regeneration.

Key words: stem cells, bone marrow mesenchymal stem cells, Ctenopharyngodon idellus, fish scale, collagen, proliferation, osteogenic differentiation

CLC Number:

Chen Liang, Meng Shu, Cheng Guoping, Ding Yi . Effects of fish scale collagen membrane on adhesion, proliferation and osteogenic differentiation of rat bone marrow mesenchymal stem cells[J]. Chinese Journal of Tissue Engineering Research, 2021, 25(16): 2494-2499.

Figures/Tables 4

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